mPEG-PDLLA/nHAP/TTCP多孔骨支架的制备及生物相容性研究
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摘要
【目的】组织工程中种子细胞需依赖于细胞外基质的存在才能发挥功能,因此支架材料的选择具有重大意义。【方法】为获得具有类似人骨的渐变孔结构、力学性能良好的仿生骨材料采用mPEG与PDLLA共聚制备改性PDLLA,并与nHAP/TTCP复合,通过控制造孔剂的粒径和用量得到不同孔径、孔隙率的mPEG-PDLLA/nHAP/TTCP有机无机复合骨支架。FT-IR考察共聚物结构,液体置换法测定支架孔隙率和密度,接触角测定法考察材料亲水性,与成骨细胞联合培养考察支架生物相容性。【结果】mPEG与PDLLA通过酯键共聚;mPEG改性PDLLA增加聚乳酸亲水性;支架的孔隙率在70%~90%,与成骨细胞相容性良好。【结论】mPEG-PDLLA/nHAP/TTCP可作为组织工程支架提供成骨细胞黏附与增殖
【Objective】Seed cells are dependent on the extracellular matrix in tissue engineering to carry out their functions, so choosing scaffold materials is of great significance.【Methods】In order to obtain tapered porous structure similar to human bone and bionic bone materials good at mechanical properties, mPEG and PDLLA were copolymerized to prepare modified PDLLA, then combined with nHAP/TTCP and obtained organic and inorganic composite bone scaffold of mPEG-PDLLA/nHAP/TTCP in different porosity and aperture by controlling the pore size and amount. FT-IR was used to determine the copolymer structure, while porosity rate and density were determined by liquid displacement method. Contact angle measurement method was used to investigate the hydrophilicity of the materials. Combined culture of scaffold and osteoblasts was used to determine the biocompatibility.【Results】Ester bond copolymerized mPEG and PDLLA; mPEG-modified PDLLA increased the hydrophilicity of poly lactic acid; the porosity rates of the scaffolds were between 70%-90%, and the compatibility with osteogenic cell was good.【Conclusion】mPEG-PDLLA/nHAP/TTCP can be used as scaffolds for tissue engineering and provide the adhesion and proliferation for osteoblasts.
【Objective】Seed cells are dependent on the extracellular matrix in tissue engineering to carry out their functions, so choosing scaffold materials is of great significance.【Methods】In order to obtain tapered porous structure similar to human bone and bionic bone materials good at mechanical properties, mPEG and PDLLA were copolymerized to prepare modified PDLLA, then combined with nHAP/TTCP and obtained organic and inorganic composite bone scaffold of mPEG-PDLLA/nHAP/TTCP in different porosity and aperture by controlling the pore size and amount. FT-IR was used to determine the copolymer structure, while porosity rate and density were determined by liquid displacement method. Contact angle measurement method was used to investigate the hydrophilicity of the materials. Combined culture of scaffold and osteoblasts was used to determine the biocompatibility.【Results】Ester bond copolymerized mPEG and PDLLA; mPEG-modified PDLLA increased the hydrophilicity of poly lactic acid; the porosity rates of the scaffolds were between 70%-90%, and the compatibility with osteogenic cell was good.【Conclusion】mPEG-PDLLA/nHAP/TTCP can be used as scaffolds for tissue engineering and provide the adhesion and proliferation for osteoblasts.
引文
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[16]祁洁,张姝江,刘宗智.整合素α5β1在成骨细胞与生物衍生材料黏附过程中的表达[J].中国组织工程研究与临床康复,2011,15(47):8762-8764.
[2]Hutmacher DW.Scaffolds in tissue engineering bone and eartilage[J].J Biomaterials,2000,21(24):2529-2543.
[3]Hing KA,Best SM,Bonfield W.Characterization of porous hydroxyapatite[J].J Mater Sci:Mater Med,1999,10(3):135-145.
[4]Chu TM,Orton DG,Hollister SJ,et al.Mechanical and in vivo performance of hydroxyapatite implants with controlled architectures[J].Biomaterials,2002,23(5):1283-1293.
[5]Hu Q,Li B,Wang M,et al.Preparation and characterization of biodegradable chitosan/hydroxyapatitenanocomposite rods via in situhybridization:a potential material as internal fixation of bone fracture[J].Biomaterials,2004,25(5):779-785.
[6]罗磊.医用胶原蛋白的临床应用[J].国外医学.口腔医学分册,1996,23(4):41.
[7]Cao W,Hench L L.Bioactive materials[J].Ceramics International,1996,22(6):493-507.
[8]陈有应,董海涛,夏亚一.羟基磷灰石吸附性能的研究进展[J].中国科技信息,2011(11):41,27.
[9]刘金标,陈建庭.磷酸三钙人工骨的研究进展[J].现代康复,2001,5(8):68-69.
[10]黄中.PLA的合成及改性研究进展[J].合成树脂及塑料,2010,6:1324-1328.
[11]顾立祥,江圣勇,何伟芳,等.PGA指数和透明质酸在诊断慢性乙肝肝纤维化中的价值[J].中国临床医学,2002,9(5):496-498.
[12]吴春波,宋学明,陈志强.透明质酸修饰的壳聚糖复合支架用于神经组织工程可行性研究[J].生物医学工程与临床,2010,14(6):477-480,封2.
[13]张华林,陈治清,李咏梅,等.电纺PLGA/HA复合支架的细胞相容性研究[J].哈尔滨医药,2011,31(5):321-322.
[14]刘文斌,陈鑫,杜梦圆,等.人工硬组织材料磷酸钙骨水泥制备方法研究[J].广东化工,2015,42(23):14-15.
[16]祁洁,张姝江,刘宗智.整合素α5β1在成骨细胞与生物衍生材料黏附过程中的表达[J].中国组织工程研究与临床康复,2011,15(47):8762-8764.