摘要
目的探讨尿前列腺癌抗原3(PCA3)基因表达水平及与血清前列腺特异性抗原(PSA)的表达水平,及其与肿瘤分化程度的关系。方法选择81例因血清PSA表达升高和(或)直肠指诊异常行前列腺穿刺活检的患者。采用苏木精-伊红(HE)染色及免疫组化鸡尾酒染色明确病理活检结果,定量-实时逆转录聚合酶链反应(qRT-PCR)检测尿PCA3 m RNA表达水平,并分析其与肿瘤分化程度的关系。前列腺癌患者尿PCA3 mRNA和血清PSA表达水平间的相关性采用线性回归分析。结果 81例患者的前列腺活检组织中,诊断前列腺癌阳性53例,阴性28例。前列腺癌患者尿PCA3 mRNA和血清PSA表达水平,均明显高于非前列腺癌患者(P﹤0.01);前列腺癌患者尿PCA3 m RNA表达水平与血清PSA表达水平无线性相关关系(P﹥0.05)。PCA3 mRNA高表达组患者Gleason评分明显高于低表达组患者(P﹤0.01),尿PCA3高表达倾向低、中度分化(P﹤0.01)。尿PCA3 mRNA高表达组患者NME1、NME3和SPARCL1 mRNA表达水平均明显低于低水平组患者(P﹤0.01);SPOCK1和survivin m RNA表达水平均明显高于低表达组患者(P﹤0.01)。结论前列腺癌患者尿PCA3 mRNA及血清PSA表达水平明显升高,但二者间无线性关系。前列腺癌患者尿PCA3 mRNA高表达时,NME1、NME3和SPARCL1mRNA表达水平及分化程度均降低,SPOCK1和survivin mRNA表达水平升高。
Objective To explore the urine prostate cancer antigen 3(PCA3) gene and serum prostate specific antigen(PSA) expression in human prostate cancer and its molecular mechanisms in tumor differentiation. Method A total of 81 samples of prostate cancer tissues were collected by diagnostic prostate puncture from patients with elevated PSA expression and(or) abnormal rectal examination results. The pathological results were determined by hematoxylin and eosin(HE) staining and immunohistochemical cocktail staining, and then quantitative realtime polymerase chain reaction(qRTPCR) was applied to detect urine PCA3 mRNA expression and analyze its association with tumor differentiation. Besides,the correlation between urine PCA3 m RNA and serum PSA expression was evaluated by linear regression analysis. Result Of the 81 patients with prostate biopsy, 53 patients of positive prostate cancer and 28 negative patients were identified.The level of urine PCA3 m RNA and serum PSA expression were significantly higher in prostate cancer patients than in those with negative lesions(P<0.01), meanwhile, there was no linear correlation between urine PCA3 mRNA and serum PSA expression in prostate cancer patients(P>0.05). Higher PCA3 mRNA was associated with higher Gleason score compared with those with lower expression(P<0.01), higher expression of urine PCA3 mRNA tended to be poorly or moderately differentiated(P<0.01). In addition, expression of urine PCA3 mRNA was negatively correlated with NME1, NME3 and SPARCL1 mRNA expression(P<0.01), and positively correlated with PCA3 mRNA, SPOCK1 and survivin mRNA expression(P<0.01). Conclusion The expression of urine PCA3 and serum PSA were higher in prostate cancer patients,though there was no linear correlation observed in between. urine PCA3 expression in prostate cancer was negatively correlated with NME1, NME3, SPARCL1 m RNA expression and the degree of differentiation, while positively correlated with SPOCK1 and survivin mRNA expression.
引文
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