摘要
目的基于27-plex SNPs族群推断体系对四川地区汉族、藏族、羌族及彝族四个族群遗传结构及成分进行研究。方法使用27-plex SNPs试剂检验四个族群1054份样本并获取SNPs位点分型,利用主成分分析、聚类分析及系统发育树综合分析族群间遗传关系,使用族群推断软件(DNA Ancestry Analyzer,DAA)推断样本的族群来源并统计推断结果的准确性。结果系统发育树显示四川汉族、藏族、羌族、彝族和其他东亚族群共同聚为一支;主成分分析和聚类分析结果显示四川汉族、藏族、羌族及彝族主要祖先成分均为东亚。除1例样本祖先来源不排除东亚或混合族群外,其余1053份样本均来源于东亚。结论四川地区汉族、藏族、羌族和彝族均为典型的东亚族群,藏族、彝族和羌族的遗传关系较近。使用27-plex SNPs族群推断系统对四川汉族、藏族、羌族和彝族进行遗传推断,结果可靠。
Objective To study the genetic structure of Han, Tibetan, Yi, and Qiang in Sichuan using the27-plex SNP panel. Methods In total, 1054 individuals of four populations from Sichuan province were tested using 27-plex SNPs. Principal component analysis, Structure and phylogenetic tree were used to analyze the genetic relationship. DNA Ancestry Analyzer(DAA) was used to analyze the ancestry components and infer the ancestry of all the test samples. Results The phylogenetic tree showed that Sichuan Han, Tibetan, Yi, Qiang were clustered with other East Asian groups. The STRUCTURE and principal component results validated that the common ancestry source of all samples from the four ethnic groups is East Asian except 1 sample. Conclusions The samples of four ethnic groups were assigned into East Asian, and Tibetan, Yi and Qiang are more genetically close, which is consistent with previous linguistics, history, and biology reports. In conclusion, the ancestry inference results of all the tested individuals from four populations in Sichuan province are reliable.
引文
[1]侯光伟,刘利民,姜先华,等.常染色体21个SNPs多态性分型方法研究[J].中国法医学杂志,2010,25(4):249-252.
[2]Wei Y L,Wei L,Zhao L,et al.A single-tube 27-plex SNPassay for estimating individual ancestry and admixture from three continents.[J].Int J Legal Med,2015,130(1):1-11.
[3]孙启凡,赵蕾,孙敬,等.利用SNP复合扩增技术推断骨骼所属人种1例[J].中国法医学杂志,2015,30(2):194-195.
[4]魏丽,魏以梁,江丽,等.27-plex SNPs复合扩增检测体系构建与应用评价[J].中国法医学杂志,2016,31(1):13-17.
[5]江丽,孙启凡,马泉,等.27-plex SNP种族推断方法的优化及验证[J].遗传,2017,39(2):166-173.
[6]刘海渤,孙启凡,陈健刚,等.27重SNP种族推断体系准确性验证研究[J].中国法医学杂志,2015,30(6):563-566.
[7]刘京,李盛,江丽,等.对于未知来源个体进行族群推断的自动分析系统[J].生命科学研究,2018,22(1):3-5.
[8]Li J,Zeng W,Zhang Y,et al.Ancient DNA reveals genetic connections between early Di-Qiang and Han Chinese[J].BMC Evol Biol.2017,17(1):239.
[9]陈仁彪,叶根跃,庚镇城,等.我国大陆主要少数民族HLA多态性聚类分析和频率分布对中华民族起源的启示[J].遗传学报,1993(5):389-398.
[10]许铭炎,洪坤学,马军,等.四川彝族和新疆维吾尔族HLA-B基因座基因多态性分析[J].遗传,2006,28(8):913-917.
[11]Qian Y,Qian B,Su B,et al.Multiple origins of Tibetan Ychromosomes.[J].Hum Genet,2000,106(4):453-454.
[12]许铭炎,陈耿臻,李军,等.四川彝族的起源初探--来自人类白细胞抗原-B基因的线索[J].汕头大学医学院学报,2005,18(4):234-237.
[13]张敏,柯立,孙上茜.明末清初“湖广填四川”人口迁徙及其影响[J].常熟理工学院学报,2008,22(5):80-82.
[14]郝伟琪,刘京,江丽,等.用于五大洲际人群区分的SNP体系研究[J].南京医科大学学报(自然科学版),2018,38(3):331-337.