引发对老化大麦籽粒吸水量及活力的影响
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摘要
为探讨聚乙二醇(PEG)和氯化钙(CaCl2)溶液对大麦老化种子活力的影响,并筛选出最适宜的大麦种子引发剂,以经过高温(40℃)、高湿(相对湿度80%)人工老化处理0,3.5,6.5d和9.5d的2个不同品种大麦种子为材料,在25℃条件下,用20%PEG溶液和1%CaCl2溶液引发处理24h,分析其吸水量、发芽率、发芽势、发芽指数、活力指数、相对电导率和丙二醛(MDA)含量的变化。结果表明:2种引发剂中,PEG溶液显著优于CaCl2溶液。2个大麦品种老化种子的吸水量、发芽率、发芽势、发芽指数和活力指数均显著提高,电导率和MDA含量显著降低。种子引发不仅能够提高其发芽特性,还能够预防种子的吸胀损伤进而修复其老化损伤。
To investigate the effects of polyethylene glycol(PEG)and calcium chloride(CaCl2)solutions on the vigor of barley aged seeds,and to screen out the most suitable barley seed initiators,in this study,two different varieties of barley seeds,which were treated with high temperature 40℃,high humidity(relative humidity 80%),artificial aging for 0d,3.5d,6.5dand 9.5d,were used as materials under the condition of 25℃.The seeds were initiated with 20% PEG solution and 1% CaCl2 solution for 24 h.The changes of water absorption,germination rate,germination potential,germination index,vigor index,relative electrical conductivity and malondialdehyde(MDA)content were analyzed.The results showed that the PEG solution was better than the CaCl2 solution in the two initiators.The water absorption,germination rate,germination potential,germination index and vigor index of aged seeds of two barley varieties increased significantly,and their electrical conductivity and MDA content decreased significantly.The seed priming could not only improve the germination characteristics of seeds,but also prevent the seed from absorbing damage and repair its aging damage.
To investigate the effects of polyethylene glycol(PEG)and calcium chloride(CaCl2)solutions on the vigor of barley aged seeds,and to screen out the most suitable barley seed initiators,in this study,two different varieties of barley seeds,which were treated with high temperature 40℃,high humidity(relative humidity 80%),artificial aging for 0d,3.5d,6.5dand 9.5d,were used as materials under the condition of 25℃.The seeds were initiated with 20% PEG solution and 1% CaCl2 solution for 24 h.The changes of water absorption,germination rate,germination potential,germination index,vigor index,relative electrical conductivity and malondialdehyde(MDA)content were analyzed.The results showed that the PEG solution was better than the CaCl2 solution in the two initiators.The water absorption,germination rate,germination potential,germination index and vigor index of aged seeds of two barley varieties increased significantly,and their electrical conductivity and MDA content decreased significantly.The seed priming could not only improve the germination characteristics of seeds,but also prevent the seed from absorbing damage and repair its aging damage.
引文
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[23]陈秉初,吴美仙,汪久康.PEG预处理对早稻种子活力和抗寒能力的影响[J].植物生理学报,1992(3):198-201.
[24]亢敏,别之龙,黄远.PEG引发对葫芦种子活力和抗氧化酶活性的影响[J].长江蔬菜,2010(8):39-41.
[25]张玲丽,郭月霞,宋喜悦.不同类型小麦品种人工老化处理后种子活力特性的研究[J].种子,2008,27(10):52-55.
[26]InayaturRahman,Ali N,Rab A,et al.Role of pre storage seed priming in controlling seed deterioration during storage.[J].Sarhad Journal of Agriculture,2013,29(3):380-386.
[27]Pandey D.Priming induced repair in French bean seeds[J].Seed Science&Technology,1988,16(2):527-532.
[28]孙艳,崔鸿文,王飞,等.PEG渗调处理对黄瓜种子活力及耐低温能力的影响[J].北方园艺,1994(6):1-3.
[29]Bailly C,Benamar A A,Corbineau B F,et al.Free radical scavenging as affected by accelerated ageing and subsequent priming in sunflower seeds[J].Physiologia Plantarum,2010,104(4):646-652.
[30]Jisha K C,Vijayakumari K,Puthur J T.Seed priming for abiotic stress tolerance:an overview[J].Acta Physiologiae Plantarum,2013,35(5):1 381-1 396.
[31]许耀照,曾秀存,王勤礼,等.PEG模拟干旱胁迫对不同黄瓜品种种子萌发的影响[J].中国蔬菜,2010,1(14):54-59.
[32]徐金金,蒋芳玲,薄丽萍,等.不同化学引发剂对不结球白菜种子引发效果的研究[J].种子,2011,30(2):8-12.
[33]Afzal I,Basra S M A,Lodhi T E,et al.Improving germination and seedling vigour in wheat by halopriming under saline conditions[J].Pakistan Journal of Agricultural Sciences,2007,44(1):41-49.
[2]Srivastava A K,Lokhande V H,Patade V Y,et al.Comparative evaluation of hydro-,chemo-,and hormonal-priming methods for imparting salt and PEG stress tolerance in Indian mustard(Brassica juncea L.)[J].Acta Physiologiae Plantarum,2010,32(6):1 135-1 144.
[3]Chen K,Arora R.Dynamics of the antioxidant system during seed osmopriming,post-priming germination,and seedling establishment in Spinach(Spinacia oleracea)[J].Plant Science An International Journal of Experimental Plant Biology,2011,180(2):212-220.
[4]张自阳,姜小苓,卢春瑞,等.PEG引发处理对人工老化小麦种子活力及幼苗生长的影响[J].河南科技学院学报(自然科学版),2013,41(1):1-5.
[5]王炜,史雨刚,王曙光.PEG引发对老化大豆种子发芽及活力的影响[J].山西农业科学,2011,39(7):650-654.
[6]徐芬芬,叶利民,王海勤,等.CaCl2浸种对水稻幼苗抗盐性的影响[J].河南农业科学,2009,38(12):44-45.
[7]王鹏,祝丽香,陈香香,等.种子引发对盐胁迫条件下桔梗种子萌发及幼苗生长的影响[J].山东农业科学,2017,49(9):71-76.
[8]杨文杰,巢思琴.不同化学药剂对黄秋葵种子的引发效果试验[J].天津农业科学,2016,22(11):115-119.
[9]马文广,崔华威,李永平,等.不同药剂引发处理对干旱胁迫下烟草种子发芽和幼苗生长的影响[J].浙江农业学报,2012,24(6):949-956.
[10]陈娟.三种药用植物种子的引发物筛选研究[D].西北农林科技大学,2010:7.
[11]胡晋.种子引发及其效应[J].种子,1998(2):33-35.
[12]Hu X W,Zhang R,Min D D,et al.Factors restricting seed germination and seedling recruitment of Ammopiptanthus mongolicus:an evergreen shrub endemic to cold deserts in China[J].Seed Science and Technology,2016,44(2):253-266.
[13]廖乐,齐军仓,华淼源,等.人工老化对大麦种子萌发过程中发芽特性及部分酶活性的影响[J].麦类作物学报,2015,35(1):71-79.
[14]Association I S T.International rules for seed testing.Rules1999.[J].Seed Science&Technology,1999,13(1):23-28.
[15]颜启传.种子学[M].中国农业出版社,2001.
[16]Farooq M,Irfan M,Aziz T,et al.Seed Priming with Ascorbic Acid Improves Drought Resistance of Wheat[J].Journal of Agronomy&Crop Science,2013,199(1):12-22.
[17]Paparella S,Araújo S S,Rossi G,et al.Seed priming:state of the art and new perspectives[J].Plant Cell Reports,2015,34(8):1 281-1 293.
[18]Manuela N,Jan K,Sibylle P,et al.Barley Seed Aging:Genetics behind the Dry Elevated Pressure of Oxygen Aging and Moist Controlled Deterioration:[J].Frontiers in Plant Science,2016,7:1-11.
[19]王彦荣,张建全,刘慧霞,等.PEG引发紫花苜蓿和沙打旺种子的生理生态效应[J].生态学报,2004,24(3):402-408.
[20]王文军.大豆种子冷害和PEG引发的蛋白质组学分析[D].内蒙古农业大学,2008:5.
[21]张海旺,芦翠乔,吴丁,等.聚乙二醇(PEG)渗透处理对老化油菜种子过氧化及细胞膜透性的影响[J].华北农学报,1989,4(2):56-62.
[22]Barreto L C,Garcia Q S.Accelerated ageing and subsequent imbibition affect seed viability and the efficiency of antioxidant system in macaw palm seeds[J].Acta Physiologiae Plantarum,2017,39(3):72.
[23]陈秉初,吴美仙,汪久康.PEG预处理对早稻种子活力和抗寒能力的影响[J].植物生理学报,1992(3):198-201.
[24]亢敏,别之龙,黄远.PEG引发对葫芦种子活力和抗氧化酶活性的影响[J].长江蔬菜,2010(8):39-41.
[25]张玲丽,郭月霞,宋喜悦.不同类型小麦品种人工老化处理后种子活力特性的研究[J].种子,2008,27(10):52-55.
[26]InayaturRahman,Ali N,Rab A,et al.Role of pre storage seed priming in controlling seed deterioration during storage.[J].Sarhad Journal of Agriculture,2013,29(3):380-386.
[27]Pandey D.Priming induced repair in French bean seeds[J].Seed Science&Technology,1988,16(2):527-532.
[28]孙艳,崔鸿文,王飞,等.PEG渗调处理对黄瓜种子活力及耐低温能力的影响[J].北方园艺,1994(6):1-3.
[29]Bailly C,Benamar A A,Corbineau B F,et al.Free radical scavenging as affected by accelerated ageing and subsequent priming in sunflower seeds[J].Physiologia Plantarum,2010,104(4):646-652.
[30]Jisha K C,Vijayakumari K,Puthur J T.Seed priming for abiotic stress tolerance:an overview[J].Acta Physiologiae Plantarum,2013,35(5):1 381-1 396.
[31]许耀照,曾秀存,王勤礼,等.PEG模拟干旱胁迫对不同黄瓜品种种子萌发的影响[J].中国蔬菜,2010,1(14):54-59.
[32]徐金金,蒋芳玲,薄丽萍,等.不同化学引发剂对不结球白菜种子引发效果的研究[J].种子,2011,30(2):8-12.
[33]Afzal I,Basra S M A,Lodhi T E,et al.Improving germination and seedling vigour in wheat by halopriming under saline conditions[J].Pakistan Journal of Agricultural Sciences,2007,44(1):41-49.