外施GA_3调控植物激素含量诱导设施甜樱桃的单性结实
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摘要
以设施甜樱桃‘美早’(Prunus avium L.‘Tieton’)为试材,研究外施10、30、60、120、300 mg·L~(-1) GA_3处理对日光温室内甜樱桃坐果率、单性结实率、果实生长发育及果实植物激素含量的影响,为外施GA_3诱导设施‘美早’甜樱桃单性结实进而提高坐果率提供基础。结果表明,外施GA_3促进了甜樱桃单花重量增加和花柄的伸长,但对花瓣长度无显著影响。外施GA_3处理显著提高了设施甜樱桃的坐果率,外施GA_3处理的设施甜樱桃果实单性结实率均为100%。外施GA_3处理促进了设施甜樱桃果实生长,果实纵径和横径变化符合双"S"生长曲线。外施GA_3改变了设施甜樱桃果实GA、IAA、CTK和ABA的含量,诱导了单性结实。外施120 mg·L~(-1) GA_3处理的综合效果最佳,具有较高的应用价值。
Tieton'sweet cherry(Prunus avium L.‘Tieton’)in greenhouse was used as experimental materials,exploring the effects of spraying GA_3 of different concentrations on fruit setting rate,parthenocarpy rate,fruit growth and development,and fruit phytohormonal content.The aim is to provide theoretical basis for improving the fruit setting rate of greenhouse sweet cherry under exogenously applied GA_3.The results showed that exogenous GA_3 promoted the increase of single flower weight and pedicel elongation,but it had no significant effect on petal elongation.Exogenously applied GA_3 could significantly improve the fruit setting rate.The parthenocarpy rate of treatments were 100%.Exogenously applied GA_3 could promote fruit growth.The variation of the vertical and horizontal diameters of the fruit conformed to the double"S"growth curve.Exogenous GA_3 altered GA,IAA,CTK and ABA content,and induced parthenocarpy.The optimum concentration of exogenous GA_3 was 120 mg·L~(-1).Therefore,spraying 120 mg·L~(-1) GA_3 significantly improved the fruit setting rate of sweet cherry in greenhouse,which can be applied and promoted in production.
Tieton'sweet cherry(Prunus avium L.‘Tieton’)in greenhouse was used as experimental materials,exploring the effects of spraying GA_3 of different concentrations on fruit setting rate,parthenocarpy rate,fruit growth and development,and fruit phytohormonal content.The aim is to provide theoretical basis for improving the fruit setting rate of greenhouse sweet cherry under exogenously applied GA_3.The results showed that exogenous GA_3 promoted the increase of single flower weight and pedicel elongation,but it had no significant effect on petal elongation.Exogenously applied GA_3 could significantly improve the fruit setting rate.The parthenocarpy rate of treatments were 100%.Exogenously applied GA_3 could promote fruit growth.The variation of the vertical and horizontal diameters of the fruit conformed to the double"S"growth curve.Exogenous GA_3 altered GA,IAA,CTK and ABA content,and induced parthenocarpy.The optimum concentration of exogenous GA_3 was 120 mg·L~(-1).Therefore,spraying 120 mg·L~(-1) GA_3 significantly improved the fruit setting rate of sweet cherry in greenhouse,which can be applied and promoted in production.
引文
Arias M,Carbonell J,AgustíM(2005).Endogenous free polyamines and their role in fruit set of low and high parthenocarpic ability citrus cultivars.J Plant Physiol,162:845-853
Aslmoshtaghi E,Shahsavar AR(2013).Study on the induction of seedless loquat.Thai J Agric Sci,46:53-57
Beech MG(1983).The induction of parthenocarpy in the strawberry cultivar redgauntlet by growth regulators.JHortic Sci Biotech,58:541-545
Chen JW,Feng JJ,Qing QP,et al(2006).Characteristics of sugar metabolism and accumulation in GA3 induced parthenocarpic while flesh loquat‘Ninghai Bai’fruit.Acta Hortic Sin,33:471-476(in Chinese with English abstract)[陈俊伟,冯健君,秦巧平等(2006).GA3诱导的单性结实‘宁海白’白沙枇杷糖代谢的研究.园艺学报,33:471-476]
Crane JC(1964).Growth substances in fruit setting and development.Annu Rev Plant Physiol,15:303-326
de Jong M,Woltersarts M,Garcíamartínez JL,et al(2011).The solanum lycopersicum AUXIN RESPONSE FAC-TOR 7(SlARF7)mediates cross-talk between auxin and gibberellin signalling during tomato fruit set and development.J Exp Bot,62:617-626
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dos Santos RC,Pereira MCT,Mendes DS,et al(2016).Gibberellic acid induces parthenocarpy and increases fruit size in the‘Gefner’custard apple(Annona cherimola×Annona squamosa).Aust J Crop Sci,10:314-321
Fan WG,An HM,Liu GQ,et al(2004).Changes of endogenous hormones contents in fruit,seeds and their effects on the fruit development of Rosa roxbunghii.Sci Agric Sin,37:728-733(in Chinese with English abstract)[樊卫国,安华明,刘国琴等(2004).刺梨果实与种子内源激素含量变化及其与果实发育的关系.中国农业科学,37:728-733]
Fos M,Nuez F,Garcia-Martinez JL(2000).The gene pat-2,which induces natural parthenocarpy,alters the gibberellin content in unpollinated tomato ovaries.Plant Physiol,122:471-479
Fos M,Proa?o K,Nuez F,et al(2001).Role of gibberellins in parthenocarpic fruit development induced by the genetic system pat-3/pat-4 in tomato.Physiol Plantarum,111:545-550
Gillaspy G,Ben-David H,Gruissem W(1993).Fruits:A developmental perspective.Plant Cell,5:1439-1451
Jung CJ,Hur YY,Jung SM,et al(2014).Transcriptional changes of gibberellin oxidase genes in grapevines with or without gibberellin application during inflorescence development.J Plant Res,127:359-371
Kong X(1987).Fruit Science Pomological of China.Beijing:China Agricultural Press,558-559(in Chinese)[孔旭(1987).中国果树栽培学.北京:农业出版社,558-559]
Li B,Li DD,Zhang J,et al(2011).Modification of resin semithin section staining method in plant tissues.Plant Physiol J,47:1207-1212(in Chinese with English abstract)[李兵,李登弟,张杰等(2011).植物树脂半薄切片染色方法的改进.植物生理学报,47:1207-1212]
Li XJ,Gao ZK,Wang M,et al(2010).Effects on PSII light absorption and utilization of CTK on the surface of tomato ovary and fruit.Acta Hortic Sin,37:749-756(in Chinese with English abstract)[李晓晶,高志奎,王梅等(2010).细胞分裂素对番茄子房和果实表面光系统Ⅱ光能吸收利用的影响.园艺学报,37:749-756]
Lu L,Liang J,Chang X,et al(2017).Enhanced vacuolar invertase activity and capability for carbohydrate import in GA-treated inflorescence correlate with increased fruit set in grapevine.Tree Genet Genomes,13:21
Lu L,Liang J,Zhu X,et al(2016).Auxin-and cytokinin-induced berries set in grapevine partly rely on enhanced gibberellin biosynthesis.Tree Genet Genomes,12:41
Mesejo C,Yuste R,Reig C,et al(2016).Gibberellin reactivates and maintains ovary-wall cell division causing fruit set in parthenocarpic Citrus species.Plant Sci,247:13-14
Ozga JA,Brenner ML,Reinecke DM(1992).Seed effects on gibberellin metabolism in peapericarp.Plant Physiol,100:88-94
Ozga JA,Yu J,Reinecke DM(2003).Pollination-,development-,and auxin-specific regulation of gibberellin 3β-hydroxylase gene expression in pea fruit and seeds.Plant Physiol,131:1137-1146
Serrani JC,Carrera E,Ruiz-Rivero O,et al(2010).Inhibition of auxin transport from the ovary or from the apical shoot induces parthenocarpic fruit-set in tomato mediated by gibberellins.Plant Physiol,153:851-862
Serrani JC,Fos M,Atarés A,et al(2007).Effect of gibberellin and auxin on parthenocarpic fruit growth induction in the cv Micro-Tom of tomato.J Plant Growth Regul,26:211-221
Serrani JC,Ruizrivero O,Fos M,et al(2008).Auxin-induced fruit-set in tomato is mediated in part by gibberellins.Plant J,56:922-934
Wang Z,Zhao F,Zhao X,et al(2012).Proteomic analysis of berry-sizing effect of GA3 on seedless Vitis vinifera L.Proteomics,12:86-94
Yamaguchi S(2008).Gibberellin metabolism and its regulation.Annu Rev Plant Biol,59:225-251
Zhang C,Lee U,Tanabe K(2008).Hormonal regulation of fruit set,parthenogenesis induction and fruit expansion in Japanese pear.Plant Growth Regul,55:231-240
Zhang XY,Song CN,Fang JG,et al(2011).Research advances in gibberellins biosynthesis and its application on grape cultivation.J Zhejiang Agric Sci,(5):1015-1018(in Chinese with English abstract)[张晓莹,宋长年,房经贵等(2011).赤霉素的生物合成及其在葡萄栽培上的应用.浙江农业科学,(5):1015-1018]
Aslmoshtaghi E,Shahsavar AR(2013).Study on the induction of seedless loquat.Thai J Agric Sci,46:53-57
Beech MG(1983).The induction of parthenocarpy in the strawberry cultivar redgauntlet by growth regulators.JHortic Sci Biotech,58:541-545
Chen JW,Feng JJ,Qing QP,et al(2006).Characteristics of sugar metabolism and accumulation in GA3 induced parthenocarpic while flesh loquat‘Ninghai Bai’fruit.Acta Hortic Sin,33:471-476(in Chinese with English abstract)[陈俊伟,冯健君,秦巧平等(2006).GA3诱导的单性结实‘宁海白’白沙枇杷糖代谢的研究.园艺学报,33:471-476]
Crane JC(1964).Growth substances in fruit setting and development.Annu Rev Plant Physiol,15:303-326
de Jong M,Woltersarts M,Garcíamartínez JL,et al(2011).The solanum lycopersicum AUXIN RESPONSE FAC-TOR 7(SlARF7)mediates cross-talk between auxin and gibberellin signalling during tomato fruit set and development.J Exp Bot,62:617-626
Ding CK,Zhang HZ(1988).The effects of plant hormone on growth and development of loquat fruit.Acta Hortic Sin,15:148-154(in Chinese)[丁长奎,章恢志(1988).植物激素对枇杷果实生长发育的影响.园艺学报,15:148-154]
Dong QQ,Gong GZ,Peng ZC,et al(2018).Analysis on the relationship between pre-harvest fruit drops and content of endogenous hormone in different parts of fruit in citrus.Plant Physiol J,54:1569-1575(in Chinese with English abstract)[董倩倩,龚桂芝,彭祝春等(2018).柑橘采前落果与果实不同部位内源激素含量关系分析.植物生理学报,54:1569-1575]
dos Santos RC,Pereira MCT,Mendes DS,et al(2016).Gibberellic acid induces parthenocarpy and increases fruit size in the‘Gefner’custard apple(Annona cherimola×Annona squamosa).Aust J Crop Sci,10:314-321
Fan WG,An HM,Liu GQ,et al(2004).Changes of endogenous hormones contents in fruit,seeds and their effects on the fruit development of Rosa roxbunghii.Sci Agric Sin,37:728-733(in Chinese with English abstract)[樊卫国,安华明,刘国琴等(2004).刺梨果实与种子内源激素含量变化及其与果实发育的关系.中国农业科学,37:728-733]
Fos M,Nuez F,Garcia-Martinez JL(2000).The gene pat-2,which induces natural parthenocarpy,alters the gibberellin content in unpollinated tomato ovaries.Plant Physiol,122:471-479
Fos M,Proa?o K,Nuez F,et al(2001).Role of gibberellins in parthenocarpic fruit development induced by the genetic system pat-3/pat-4 in tomato.Physiol Plantarum,111:545-550
Gillaspy G,Ben-David H,Gruissem W(1993).Fruits:A developmental perspective.Plant Cell,5:1439-1451
Jung CJ,Hur YY,Jung SM,et al(2014).Transcriptional changes of gibberellin oxidase genes in grapevines with or without gibberellin application during inflorescence development.J Plant Res,127:359-371
Kong X(1987).Fruit Science Pomological of China.Beijing:China Agricultural Press,558-559(in Chinese)[孔旭(1987).中国果树栽培学.北京:农业出版社,558-559]
Li B,Li DD,Zhang J,et al(2011).Modification of resin semithin section staining method in plant tissues.Plant Physiol J,47:1207-1212(in Chinese with English abstract)[李兵,李登弟,张杰等(2011).植物树脂半薄切片染色方法的改进.植物生理学报,47:1207-1212]
Li XJ,Gao ZK,Wang M,et al(2010).Effects on PSII light absorption and utilization of CTK on the surface of tomato ovary and fruit.Acta Hortic Sin,37:749-756(in Chinese with English abstract)[李晓晶,高志奎,王梅等(2010).细胞分裂素对番茄子房和果实表面光系统Ⅱ光能吸收利用的影响.园艺学报,37:749-756]
Lu L,Liang J,Chang X,et al(2017).Enhanced vacuolar invertase activity and capability for carbohydrate import in GA-treated inflorescence correlate with increased fruit set in grapevine.Tree Genet Genomes,13:21
Lu L,Liang J,Zhu X,et al(2016).Auxin-and cytokinin-induced berries set in grapevine partly rely on enhanced gibberellin biosynthesis.Tree Genet Genomes,12:41
Mesejo C,Yuste R,Reig C,et al(2016).Gibberellin reactivates and maintains ovary-wall cell division causing fruit set in parthenocarpic Citrus species.Plant Sci,247:13-14
Ozga JA,Brenner ML,Reinecke DM(1992).Seed effects on gibberellin metabolism in peapericarp.Plant Physiol,100:88-94
Ozga JA,Yu J,Reinecke DM(2003).Pollination-,development-,and auxin-specific regulation of gibberellin 3β-hydroxylase gene expression in pea fruit and seeds.Plant Physiol,131:1137-1146
Serrani JC,Carrera E,Ruiz-Rivero O,et al(2010).Inhibition of auxin transport from the ovary or from the apical shoot induces parthenocarpic fruit-set in tomato mediated by gibberellins.Plant Physiol,153:851-862
Serrani JC,Fos M,Atarés A,et al(2007).Effect of gibberellin and auxin on parthenocarpic fruit growth induction in the cv Micro-Tom of tomato.J Plant Growth Regul,26:211-221
Serrani JC,Ruizrivero O,Fos M,et al(2008).Auxin-induced fruit-set in tomato is mediated in part by gibberellins.Plant J,56:922-934
Wang Z,Zhao F,Zhao X,et al(2012).Proteomic analysis of berry-sizing effect of GA3 on seedless Vitis vinifera L.Proteomics,12:86-94
Yamaguchi S(2008).Gibberellin metabolism and its regulation.Annu Rev Plant Biol,59:225-251
Zhang C,Lee U,Tanabe K(2008).Hormonal regulation of fruit set,parthenogenesis induction and fruit expansion in Japanese pear.Plant Growth Regul,55:231-240
Zhang XY,Song CN,Fang JG,et al(2011).Research advances in gibberellins biosynthesis and its application on grape cultivation.J Zhejiang Agric Sci,(5):1015-1018(in Chinese with English abstract)[张晓莹,宋长年,房经贵等(2011).赤霉素的生物合成及其在葡萄栽培上的应用.浙江农业科学,(5):1015-1018]