摘要
植物VQ蛋白作为植物体内一类重要的辅助调控因子,对植物生长发育、基因调控以及响应外界环境变化方面发挥重要作用。探讨激素及非生物胁迫下OsVQ20基因的表达模式,为水稻的品种改良提供理论依据。通过PlantCARE数据对OsVQ20基因启动子元件进行分析,利用水稻激素芯片及荧光定量PCR分析OsVQ20基因的表达模式。结果表明,OsVQ20基因启动子含有干旱(MBS)、低温(LTR)、脱落酸(ABRS)、生长素(AuxRR-core)等多种应答元件;OsVQ20基因的表达受油菜素内酯(BL)、茉莉酸(JA)的诱导,受生长素(IAA)、细胞分裂素(CTK)以及脱落酸(ABA)的抑制作用,在赤霉素(GA)处理下无明显变化。在非生物胁迫下,OsVQ20基因明显受干旱和盐胁迫的诱导,受低温和氧化胁迫的抑制,高温胁迫下无显著变化。该研究为进一步研究OsVQ20基因的生物学功能及水稻抗逆育种提供理论依据。
Plant VQ protein, as an important auxiliary regulator in plants, plays an important role in plant growth and development, gene regulation and response to environmental changes. The expression pattern of OsVQ20 gene was investigated under hormone and abiotic stress, so as to provide a theoretical basis for breed improvement of rice. The promoter elements of OsVQ20 gene were analyzed by PlantCARE data, and the expression pattern of OsVQ20 gene was analyzed by rice hormone chip and fluorescence quantitative PCR. The results showed that the promoter of OsVQ20 gene contained multiple response elements, including low temperature response element(LTR), drought responsive element(MBS), abscisic acid response element(ABRS), and auxin response element(AuxRR-core). The expression of OsVQ20 was induced by brassinolide(BL) and jasmonic acid(JA) and inhibited by IAA, cytokinin(CTK) and abscisic acid(ABA), but there was no significant changes under gibberellin treatment.Under abiotic stress, OsVQ20 gene was induced by drought and salt stress, inhibited by low temperature and oxidative stress, but had no significant change under high temperature stress. This study could lay a foundation for further study of the biological functions of OsVQ20 and breeding of rice with resistance.
引文
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