前列腺癌细胞系及组织中α-1,6岩藻糖基转移酶的表达及意义
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摘要
目的探讨α-1,6岩藻糖基转移酶(α-1,6fucocyltransferase,FUT8)在不同前列腺癌细胞系、癌及癌旁组织中的表达差异及其意义。方法培养前列腺正常上皮细胞(RWPE-1)、4种前列腺癌细胞(22RV1、LNCap、PC-3、DU145)及2种其他类型泌尿系统肿瘤细胞(T-24、786-O),收集15对前列腺癌及癌旁组织,提取总RNA,采用RT-PCR检测不同细胞系、癌及癌旁组织中FUT8mRNA的表达,并进行差异比较。结果 FUT8 mRNA在前列腺癌细胞系中的表达高于前列腺正常上皮细胞系(RWPE-1)、膀胱癌细胞系(T-24)以及肾癌细胞系(786-O)。雄激素非依赖性前列腺癌细胞系(PC-3、DU145)中FUT8 mRNA的表达高于雄激素依赖性前列腺癌细胞系(LNCap);前列腺癌转移灶细胞系(PC-3、DU145)中FUT8mRNA的表达高于前列腺癌原发灶细胞系(22RV1)。同时还发现,FUT8mRNA在前列腺癌组织中的表达高于癌旁组织。以上差异均有统计学意义(P<0.01)。结论 FUT8在不同前列腺癌细胞系、癌及癌旁组织中存在差异表达,可能参与前列腺癌的雄激素非依赖性转化及进展转移等过程。
Objective To investigate the differential expression and significance ofα-1,6 fucocyltransferase(FUT8)in prostate cancer cell lines with different biological characteristics and tissues. Methods The normal epithelial cells of prostate(RWPE-1),four types of prostate cancer cells(22 RV1,LNCap,PC-3,DU145)and the other two types of urological cancer cells(T-24,786-O)were cultured.Fifteen pairs of prostatic cancerous and paracancerous tissues were collected.The expression levels of FUT8 mRNA in the different cell lines and tissues were detected and evaluated by reverse transcription-polymerase chain reaction(RT-PCR). Results The expression levels of FUT8 mRNA in prostate cancer cell lines were significantly higher than that in normal epithelial cell line of prostate(RWPE-1),bladder cancer cell line(T-24)and renal cell carcinoma cell line(786-O).FUT8 mRNA was detected with higher levels of expression in PC-3 and DU145 cell lines which were both known as AI-PCa cell lines than that in LNCap cell line which was known as AD-PCa cell line.The expression levels of FUT8 mRNA in PC-3 and DU-145 cell lines which were both from metastases were apparently higher than that in 22 RV1 cell line which was from primary tumor.It was also found that FUT8 mRNA was up-regulated in prostatic cancerous tissues than in paracancerous tissues.The differences were all significant(P<0.01). Conclusions There was remarkable difference in the expression of FUT8 in prostate cancer cell lines with different biological characteristics and tissues,indicating that FUT8 may be involved in the conversion process from AD-PCa to AI-PCa and influence the progression and metastasis of prostate cancer.
Objective To investigate the differential expression and significance ofα-1,6 fucocyltransferase(FUT8)in prostate cancer cell lines with different biological characteristics and tissues. Methods The normal epithelial cells of prostate(RWPE-1),four types of prostate cancer cells(22 RV1,LNCap,PC-3,DU145)and the other two types of urological cancer cells(T-24,786-O)were cultured.Fifteen pairs of prostatic cancerous and paracancerous tissues were collected.The expression levels of FUT8 mRNA in the different cell lines and tissues were detected and evaluated by reverse transcription-polymerase chain reaction(RT-PCR). Results The expression levels of FUT8 mRNA in prostate cancer cell lines were significantly higher than that in normal epithelial cell line of prostate(RWPE-1),bladder cancer cell line(T-24)and renal cell carcinoma cell line(786-O).FUT8 mRNA was detected with higher levels of expression in PC-3 and DU145 cell lines which were both known as AI-PCa cell lines than that in LNCap cell line which was known as AD-PCa cell line.The expression levels of FUT8 mRNA in PC-3 and DU-145 cell lines which were both from metastases were apparently higher than that in 22 RV1 cell line which was from primary tumor.It was also found that FUT8 mRNA was up-regulated in prostatic cancerous tissues than in paracancerous tissues.The differences were all significant(P<0.01). Conclusions There was remarkable difference in the expression of FUT8 in prostate cancer cell lines with different biological characteristics and tissues,indicating that FUT8 may be involved in the conversion process from AD-PCa to AI-PCa and influence the progression and metastasis of prostate cancer.
引文
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[2]Siegel R,Naishadham D,Jemal A.Cancer statistics,2013[J].CA Cancer J Clin,2013,63(1):11-30.
[3]Chen W,Zheng R,Baade PD,et al.Cancer statistics in China,2015[J].CA Cancer J Clin,2016,66(2):115-132.
[4]中华医学会泌尿外科学分会前列腺癌联盟.中国前列腺癌早期诊断专家共识[J].中华泌尿外科杂志,2015,36(8):561-564.
[5]韩苏军,张思维,陈万青,等.中国前列腺癌发病现状和流行趋势分析[J].临床肿瘤学杂志,2013,18(4):330-334.
[6]韩苏军,张思维,陈万青,等.中国前列腺癌死亡现状及流行趋势分析[J].中华泌尿外科杂志,2012,33(11):836-839.
[7]Sato Y,Nakata K,Kato Y,et al.Early recognition of hepatocellular carcinoma based on altered profiles of alpha-fetoprotein[J].N Engl J Med,1993,328(25):1802-1806.
[8]Takeda Y,Shinzaki S,Okudo K,et al.Fucosylated haptoglobin is a novel type of cancer biomarker linked to the prognosis after an operation in colorectal cancer[J].Cancer,2012,118(12):3036-3043.
[9]Chen CY,Jan YH,Juan YH,et al.Fucosyltransferase 8as a functional regulator of nonsmall cell lung cancer[J].Proc Natl Acad Sci USA,2013,110(2):630-635.
[10]Ju L,Wang Y,Xie Q,et al.Elevated level of serum glycoprotein bifucosylation and prognostic value in Chinese breast cancer[J].Glycobiology,2016,26(5):460-471.
[11]Miyoshi E,Kamada Y.Application of glycoscience to the early detection of pancreatic cancer[J].Cancer Sci,2016,107(10):1357-1362.
[12]Taniguchi N,Kizuka Y.Glycans and cancer:role of N-glycans in cancer biomarker,progression and metastasis,and therapeutics[J].Adv Cancer Res,2015,126:11-51.
[13]HSIEH Jer-Tsong,吴开杰,杨林,等.雄激素非依赖性前列腺癌发生的机制[J].现代泌尿外科杂志,2010,15(6):401-407.