革兰氏阴性菌TonB-ExbB-ExbD复合物的功能、作用机制、分布及进化
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摘要
革兰氏阴性菌在生长繁殖过程中需要从外界摄取营养物质。一些小分子营养物质可以自由地通过革兰氏阴性菌的细胞膜,而一些大分子营养物质的转运需要特异性的TonB复合物依赖性的外膜受体进行转运。TonB复合物由TonB、ExbB、ExbD构成,是革兰氏阴性菌对外界营养物质主动转运过程的能量提供单位,在革兰氏阴性菌分布广泛。近年来,对TonB-ExbB-ExbD复合物的功能、结构及作用机制取得了重大研究进展,然而此复合物在不同的细菌也存在功能及作用机制上的差异。基于此背景,本文综述了TonB复合物的功能和结构研究进展,并分析了TonB复合物在革兰氏阴性菌中的分布、进化,比较了不同革兰氏阴性菌此复合物的差异,有助于进一步发现和揭示TonB复合物的新功能与作用机制。
Gram-negative bacteria need to take nutrients from outside during growth and proliferation.Some small molecule nutrients can pass freely through the cell membrane of Gram-negative bacteria,while the transport of some macromolecular nutrients require specific TonB complex-dependent outer membrane receptors. The TonB complex are widely distributed in Gram-negative bacteria,which are composed of TonB,ExbB,and ExbD and acts as the energy supplying unit for the active transport of external macromolecular nutrients. In recent years,significant advances have been made in the study of the functions,structures,and mechanisms of the TonB-ExbB-ExbD complex. However,there are some differences in the functions and mechanisms of the TonB-ExbB-ExbD complex in different bacteria. In this paper,we review the function and structure of the TonB complex,and analyze the distribution and evolution of the TonB complex in Gram-negative bacteria,and compare the differences among the complexes of different Gram-negative bacteria. It is helpful to further discover and reveal the newfunctions and mechanisms of the TonB complex.
Gram-negative bacteria need to take nutrients from outside during growth and proliferation.Some small molecule nutrients can pass freely through the cell membrane of Gram-negative bacteria,while the transport of some macromolecular nutrients require specific TonB complex-dependent outer membrane receptors. The TonB complex are widely distributed in Gram-negative bacteria,which are composed of TonB,ExbB,and ExbD and acts as the energy supplying unit for the active transport of external macromolecular nutrients. In recent years,significant advances have been made in the study of the functions,structures,and mechanisms of the TonB-ExbB-ExbD complex. However,there are some differences in the functions and mechanisms of the TonB-ExbB-ExbD complex in different bacteria. In this paper,we review the function and structure of the TonB complex,and analyze the distribution and evolution of the TonB complex in Gram-negative bacteria,and compare the differences among the complexes of different Gram-negative bacteria. It is helpful to further discover and reveal the newfunctions and mechanisms of the TonB complex.
引文
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[2] Ge R, Sun X. Iron acquisition and regulation systems in Streptococcus species[J]. Metallomics,2014,6(5):996-1003
[3] Nikaido H. Molecular basis of bacterial outer membrane permeability revisited[J]. Microbiol Mol Biol Rev,2003,67(4):593-656
[4] Ollis AA,Postle K. ExbD mutants define initial stages in TonB energization[J]. J Mol Biol,2012,415(2):237-247
[5] Schauer K,Rodionov DA,de Reuse H. New substrates for TonB-dependent transport:do we only see the‘tip of the iceberg’?[J]. Trends Biochem Sci,2008,33(7):330-338
[6] Braun V. Energy-coupled transport and signal transduction through the gram-negative outer membrane via TonB-ExbB-ExbDdependent receptor proteins[J]. FEMS Microbiol Rev,1995,16(4):295-307
[7] Cascales E,Lloubès R,Sturgis JN. The Tol Q-TolR proteins energize Tol A and share homologies with the flagellar motor proteins Mot A-MotB[J]. Mol Microbiol,2001,42(3):795-807
[8] Celia H,Noinaj N,Zakharov SD,et al. Structural insight into the role of the Ton complex in energy transduction[J]. Nature,2016,538(7623):60-65
[9] Noinaj N, Guillier M, Barnard TJ, et al. TonB-dependent transporters:regulation,structure,and function[J]. Annu Rev Microbiol,2010,64:43-60
[10] Gresock MG, Kastead KA, Postle K. From homodimer to heterodimer and back:elucidating the TonB energy transduction cycle[J]. J Bacteriol,2015,197(21):3433-3445
[11] Lohmiller S, Hantke K, Patzer SI, et al. TonB-dependent maltose transport by Caulobacter crescentus[J]. Microbiology,2008,154(Pt 6):1748-1754
[12] Wayne R,Neilands JB. Evidence for common binding sites for ferrichrome compounds and bacteriophage phi 80 in the cell envelope of Escherichia coli[J]. J Bacteriol,1975,121(2):497-503
[13] Hantke K. Identification of an iron uptake system specific for coprogen and rhodotorulic acid in Escherichia coli K12[J]. Mol Gen Genet,1983,191(2):301-306
[14] Hollifield WC Jr,Fiss EH,Neilands JB. Modification of a ferric enterobactin receptor protein from the outer membrane of Escherichia coli[J]. Biochem Biophys Res Commun,1978,83(2):739-746
[15] Wookey P, Rosenberg H. Involvement of inner and outer membrane components in the transport of iron and in colicin B action in Escherichia coli[J]. J Bacteriol,1978,133(2):661-666
[16] Nikaido H,Rosenberg EY. Cir and Fiu proteins in the outer membrane of Escherichia coli catalyze transport of monomeric catechols:study with beta-lactam antibiotics containing catechol and analogous groups[J]. J Bacteriol, 1990, 172(3):1361-1367
[17] de Lorenzo V, Bindereif A, Paw BH, et al. Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12[J]. J Bacteriol,1986,165(2):570-578
[18] Wagegg W,Braun V. Ferric citate transport in Escherichia coli requires outer membrane receptor protein fec A[J]. J Bacteriol,1981,145(1):156-163
[19] Porcheron G,Garénaux A,Proulx J,et al. Iron,copper,zinc,and manganese transport and regulation in pathogenic Enterobacteria:correlations between strains,site of infection and the relative importance of the different metal transport systems for virulence[J]. Front Cell Infect Microbiol,2013,3:90
[20] Balhesteros H,Shipelskiy Y,Long NJ,et al. TonB-Dependent Heme/Hemoglobin Utilization by Caulobacter crescentus HutA[J]. J Bacteriol,2017,199(6).pii:e00723-16
[21] Bassford PJ Jr,Kadner RJ. Genetic analysis of components involved in vitamin B12 uptake in Escherichia coli[J]. J Bacteriol,1977,132(3):796-805
[22] Schwiesow L,Mettert E,Wei Y,et al. Control of hmu Heme Uptake Genes in Yersinia pseudotuberculosis in Response to Iron Sources[J]. Front Cell Infect Microbiol,2018,8:47
[23] Schauer K,Gouget B,Carriere M,et al. Novel nickel transport mechanism across the bacterial outer membrane energized by the TonB/ExbB/ExbD machinery[J]. Mol Microbiol, 2007, 63(4):1054-1068
[24] Si M,Zhao C,Burkinshaw B,et al. Manganese scavenging and oxidative stress response mediated by type VI secretion system in Burkholderia thailandensis[J]. Proc Natl Acad Sci U S A,2017,114(11):E2233-E2242
[25] Si M,Wang Y,Zhang B,et al. The Type VI Secretion System Engages a Redox-Regulated Dual-Functional Heme Transporter for Zinc Acquisition[J]. Cell Rep,2017,20(4):949-959
[26] Abdollahi S, Rasooli I, Mousavi Gargari SL. An in silico structural and physicochemical characterization of TonBdependent copper receptor in A. baumannii[J]. Microb Pathog,2018,118:18-31
[27] Abdollahi S,Rasooli I,Mousavi Gargari SL. The role of TonBdependent copper receptor in virulence of Acinetobacter baumannii[J]. Infect Genet Evol,2018,60:181-190
[28] Burrows LL. A new route for polar navigation[J]. Mol Microbiol,2013,90(5):919-922
[29] Alice AF,Naka H,Crosa JH. Global gene expression as a function of the iron status of the bacterial cell:influence of differentially expressed genes in the virulence of the human pathogen Vibrio vulnificus[J]. Infect Immun,2008,76(9):4019-4037
[30] Duong-Nu TM,Jeong K,Hong SH,et al. All Three Ton B Systems Are Required for Vibrio vulnificus CMCP6 Tissue Invasiveness by Controlling Flagellum Expression[J]. Infect Immun,2015,84(1):254-265
[31] Huang B,Ru K,Yuan Z,et al. tonB3 is required for normal twitching motility and extracellular assembly of type IV pili[J]. J Bacteriol,2004,186(13):4387-4389
[32] Rosenberg T,Salam BB,Burdman S. Association between loss of type IV pilus synthesis ability and phenotypic variation in the cucurbit pathogenic bacterium Acidovorax citrulli[J]. Mol Plant Microbe Interact,2018. 31(5):548-559
[33] Koebnik R. TonB-dependent trans-envelope signalling:the exception or the rule?[J]. Trends Microbiol,2005,13(8):343-347
[34] Wang Q,Liu Q,Cao X,et al. Characterization of two TonB systems in marine fish pathogen Vibrio alginolyticus:their roles in iron utilization and virulence[J]. Arch Microbiol,2008,190(5):595-603
[35] Ferguson AD, Amezcua CA, Halabi NM, et al. Signal transduction pathway of TonB-dependent transporters[J]. Proc Natl Acad Sci U S A,2007,104(2):513-518
[36] Braun V,Braun M. Iron transport and signaling in Escherichia coli[J]. FEBS Lett,2002,529(1):78-85
[37] Llamas MA,Imperi F,Visca P,et al. Cell-surface signaling in Pseudomonas:stress responses,iron transport,and pathogenicity[J]. FEMS Microbiol Rev,2014,38(4):569-597
[38] Brickman TJ,Vanderpool CK,Armstrong SK. Heme transport contributes to in vivo fitness of Bordetella pertussis during primary infection in mice[J]. Infect Immun,2006,74(3):1741-1744
[39] Liao H,Cheng X,Zhu D,et al. TonB Energy Transduction Systems of Riemerella anatipestifer Are Required for Iron and Hemin Utilization[J]. PLoS One,2015,10(5):e0127506
[40] Miao S,Xing L,Qi J,et al. Roles of the TonB1 and TonB2proteins in haemin iron acquisition and virulence in Riemerella anatipestifer[J]. Microbiology,2015,161(8):1592-1599
[41] Zimbler DL,Arivett BA,Beckett AC,et al. Functional Features of Ton B Energy Transduction Systems of Acinetobacter baumannii[J]. Infect Immun,2013,81(9):3382-3394
[42] Minandri F,Imperi F,Frangipani E,et al. Role of Iron Uptake Systems in Pseudomonas aeruginosa Virulence and Airway Infection[J]. Infect Immun,2016,84(8):2324-2335
[43] Bosak J,Laiblova P,Smarda J,et al. Novel colicin Fy of Yersinia frederiksenii inhibits pathogenic Yersinia strains via YiuR-mediated reception,TonB import,and cell membrane pore formation[J]. J Bacteriol,2012,194(8):1950-1959
[44] Sverzhinsky A,Chung JW,Deme JC,et al. Membrane Protein Complex ExbB4-ExbD1-TonB1from Escherichia coli Demonstrates Conformational Plasticity[J]. J Bacteriol,2015,197(11):1873-1885
[45] Krewulak KD,Vogel HJ. TonB or not Ton B:is that the question?[J]. Biochem Cell Biol,2011,89(2):87-97
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