基于响应曲面法优化肠内全营养乳剂的初乳剪切工艺
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摘要
全营养组方中选用酪蛋白酸钠和乳清蛋白作为蛋白质的来源,选用紫苏油作为n-3不饱和脂肪酸的来源,制备特殊医学用途配方食品肠内营养乳剂,并对其剪切工艺参数进行研究。使用响应面法对剪切工艺参数进行优化,以D50和跨距为响应值,剪切时间、剪切转速、剪切温度为3个变量因素进行实验设计。经验证实验,得到最佳剪切工艺条件为:剪切转速4000 r/min,剪切时间22.5 min,剪切温度25℃。稳定性实验表明,经剪切工艺优化条件制备得到的肠内营养乳剂具有较好的稀释稳定性和离心稳定性。
Response Surface Method(RSM)is used to optimize the process parameters in preparation of total enteral nutritional emulsion. In the formulation,whey protein and sodium caseinate are used as the source of protein,perilla oil is used as the source of n-3 unsaturated fatty acid. The D50 and span value were used as the response values,and the shear time,shear speed and shear temperature were used as variables to conduct the experiment. The experiment was carried out to obtain the optimized process parameters in preparation of the emulsion. Result shows that when shear speed was at 4000 r/min,shear time at 22.5 min and shear temperature at 25 ℃,the target emulsion was most stable as described by its dilution and centrifugal stability.
Response Surface Method(RSM)is used to optimize the process parameters in preparation of total enteral nutritional emulsion. In the formulation,whey protein and sodium caseinate are used as the source of protein,perilla oil is used as the source of n-3 unsaturated fatty acid. The D50 and span value were used as the response values,and the shear time,shear speed and shear temperature were used as variables to conduct the experiment. The experiment was carried out to obtain the optimized process parameters in preparation of the emulsion. Result shows that when shear speed was at 4000 r/min,shear time at 22.5 min and shear temperature at 25 ℃,the target emulsion was most stable as described by its dilution and centrifugal stability.
引文
[1]韩军花.特殊医学用途配方食品系列标准实施指南[M].北京:中国质检出版社、中国标准出版社,2015.
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[7]Sigalet D L,Mackenzie S L,Hameed S M. Enteral nutrition and mucosal immunity:implications for feeding strategies in surgery and trauma[J]. Canadian Journal of Surgery,2004,47(2):109-116.
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[14]张艳,李永哲.响应面法及其在药学领域中的应用[J].吉林化工学院学报,2012,29(7):20-26.
[15]张泽志,韩春亮,李成未.响应面法在试验设计与优化中的应用[J].河南教育学院学报(自然科学版),2011,20(4):34-37.
[16]Shibata H,Saito H,Yomota C,et al. Pharmaceutical quality evaluation of lipid emulsions containing PGE1:Alteration in the number of large particles in infusion solutions[J]. International Journal of Pharmaceutics,2009,378(1-2):167-176.
[17]陈祝康,陈桂良,李慧义.药物及其制剂粒度测定法探讨[J].中国药品标准,2012,13(6):405-408.
[18]马萍,辛艳茹,杨京燕,等.离心分光光度法测定乳剂的稳定性[J].药学实践杂志,2001,19(1):23-24.
[19]Michels R,Foschum F,Kienle A. Optical properties of fat emulsions[J]. Optics Express,2008,16(8):5907-5925.
[20]Poullain-Termeau,Stéphanie,Crauste-Manciet S,Brossard D,et al. Effect of Oil-in-Water Submicron Emulsion Surface Charge on Oral Absorption of a Poorly Water-Soluble Drug in Rats[J]. Drug Delivery,2008,15(8):503-514.
[21]Dey T K,Ghosh S,Ghosh M,et al. Comparative study of gastrointestinal absorption of EPA&DHA rich fish oil from nano and conventional emulsion formulation in rats[J]. Food Research International,2012,49(1):72-79.
[22]Parlak M,Osturk O,Temel U N,et al. Heat transfer performance of water based nanofluids containing various types of metal oxide nanoparticles in an air-cooled microchannel heat exchanger[C]. Thermal&Thermomechanical Phenomena in Electronic Systems. IEEE,2017.
[2]Philipson TJ,Snider JT,Lakdawalla DN,et al. Impact of oral nutritional supplementation on hospital outcomes[J]. Am J Manag Care. 2013,19(2):121-128.
[3]Laviano A,Norman K,Elia U,et al. A systematic review of the cost and cost effectiveness of using standard oral nutritional supplements in the hospital setting[J]. Clinical Nutrition,2016,35(2):370-380.
[4]A meta analysis of treatment outcomes of early enteral versus early parenteral nutrition in hospitalized patients[J]. Crit Care Med,2005,33(1):213-220.
[5]Okamoto K,Fukatsu K,et al. Tlymphocyte numbers inhuman gutassociated lymphoid tissue are reduced without enteral nutrition[J]. JPEN J Parenter Enteral Nutr,2005,29(1):56-58.
[6]潘炳权,王伟,葛晓军.早期肠内营养支持对胃癌根治术后患者的疗效观察[J].中国普通外科杂志,2011,20(7):778-780.
[7]Sigalet D L,Mackenzie S L,Hameed S M. Enteral nutrition and mucosal immunity:implications for feeding strategies in surgery and trauma[J]. Canadian Journal of Surgery,2004,47(2):109-116.
[8]李晓晖.牛乳中酪蛋白的结构特性及其应用[J].食品工业,2002(1):29-31.
[9]梁琪.酪蛋白酸钠功能性的研究[J].食品科学,2002,23(3):30-33.
[10]孙向东,李硕.蛋白质乳化作用与置换吸附探讨[J].食品工业科技,2012,33(16):405-408.
[11]GB29922-2013,食品安全国家标准特殊医学用途配方食品通则[S].中国:中华人民共和国国家卫生和计划生育委员会,2013.
[12]申孙奇.脂肪乳的制备及质量评价[J].医学信息,2012,25(1):391-391.
[13]王永菲,王成国.响应面法的理论与应用[J].中央民族大学学报(自然科学版),2005,14(3):236-240.
[14]张艳,李永哲.响应面法及其在药学领域中的应用[J].吉林化工学院学报,2012,29(7):20-26.
[15]张泽志,韩春亮,李成未.响应面法在试验设计与优化中的应用[J].河南教育学院学报(自然科学版),2011,20(4):34-37.
[16]Shibata H,Saito H,Yomota C,et al. Pharmaceutical quality evaluation of lipid emulsions containing PGE1:Alteration in the number of large particles in infusion solutions[J]. International Journal of Pharmaceutics,2009,378(1-2):167-176.
[17]陈祝康,陈桂良,李慧义.药物及其制剂粒度测定法探讨[J].中国药品标准,2012,13(6):405-408.
[18]马萍,辛艳茹,杨京燕,等.离心分光光度法测定乳剂的稳定性[J].药学实践杂志,2001,19(1):23-24.
[19]Michels R,Foschum F,Kienle A. Optical properties of fat emulsions[J]. Optics Express,2008,16(8):5907-5925.
[20]Poullain-Termeau,Stéphanie,Crauste-Manciet S,Brossard D,et al. Effect of Oil-in-Water Submicron Emulsion Surface Charge on Oral Absorption of a Poorly Water-Soluble Drug in Rats[J]. Drug Delivery,2008,15(8):503-514.
[21]Dey T K,Ghosh S,Ghosh M,et al. Comparative study of gastrointestinal absorption of EPA&DHA rich fish oil from nano and conventional emulsion formulation in rats[J]. Food Research International,2012,49(1):72-79.
[22]Parlak M,Osturk O,Temel U N,et al. Heat transfer performance of water based nanofluids containing various types of metal oxide nanoparticles in an air-cooled microchannel heat exchanger[C]. Thermal&Thermomechanical Phenomena in Electronic Systems. IEEE,2017.