海藻酸盐共混泊洛沙姆的可注射水凝胶的制备及表征
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摘要
以海藻酸钠(SA)共混泊洛沙姆(P407和P188)制备机械性能较高的可注射原位凝胶;以SA和CaCO_3交联形成凝胶,共混泊洛沙姆制备可注射凝胶。通过对混合凝胶的微观结构、机械强度及生物学进行了表征。结果表明,通过化学与物理双重交联,混合凝胶形成了多孔均匀的微观结构,混合凝胶的抗压缩性能及弹性有所提高;流变学显示其弹性成分大于黏性成分;通过XRD实验,表明混合凝胶的稳定性提高;同时MTT实验证明,细胞增殖率为95%,表明混合凝胶对机体无毒性。通过此方法制备的混合凝胶既提高了机械性能,同时又具备温度响应性和可注射性。
The injectable in-situ hydrogel was fabricated based on sodium alginate as the basic material combined with Poloxamers(P407 and P188).The microstructure,stability,mechanical strength and biocompatibility of the mixed hydrogel were characterized.The results showed that composite gel mixed with SA,P407 and P188 has a more porous and homogeneous microstructure,good compressive resistance to external force based on the chemical-physical crosslinking.The stability of the gel was enhanced by Ca-SA combined with Poloxamer through TG analysis.The MTT experiment proves nontoxic to the body with cell viability 95%.Taken together,the composite hydrogel not only enhanced the mechanical strength,but has characters of temperature responsiveness and injectable.
The injectable in-situ hydrogel was fabricated based on sodium alginate as the basic material combined with Poloxamers(P407 and P188).The microstructure,stability,mechanical strength and biocompatibility of the mixed hydrogel were characterized.The results showed that composite gel mixed with SA,P407 and P188 has a more porous and homogeneous microstructure,good compressive resistance to external force based on the chemical-physical crosslinking.The stability of the gel was enhanced by Ca-SA combined with Poloxamer through TG analysis.The MTT experiment proves nontoxic to the body with cell viability 95%.Taken together,the composite hydrogel not only enhanced the mechanical strength,but has characters of temperature responsiveness and injectable.
引文
[1] Allen O Osaheni,Eric B Finkelstein,Patrick T Mather,et al.Synthesis and characterization of a zwitterionic hydrogel blend with low coefficient of friction[J].Acta Biomaterials,2016,46(11):245-255.
[2] Muhammad Rizwan,Gary S L Peh,Heng-Pei Ang,et al.Sequentially-crosslinked bioactive hydrogel as nano-patterned substrate with customizable stiffness and degradation for corneal tissue engineering application[J].Biomaterials,2017,120(3):139-154.
[3] 王津,陈莉敏,蒋智清,等.壳聚糖-明胶体系的温敏凝胶及其药物缓释性能[J].功能材料,2013,44(9):1294-1297.
[4] 王波,刁路明.胶原/聚乙烯醇凝胶的制备及生物相容性评价[J].郧阳医学院学报,2010,29(4):335-338.
[5] Friedrich K,Karger-Kocsis J.On the sliding wear performance of polyethernitrile composites[J].Wear,1992,158(1/2):157-170.
[6] Michelle M Blum,Timothy C Ovaert.Investigation of friction and surface degradation of innovative boundary lubricant functionalized hydrogel material for use as artificial articular cartilage[J].Wear,2013,301(1/2):201-209.
[7] 魏宏亮,王连才,张爱英,等.可注射水凝胶的制备与应用[J].化学进展,2004,16(6):1009-1016.
[8] Alessandra C S Akkari,Juliana Z B Papini,Gabriella K Garcia,et al.Poloxamer 407/188 binary thermosensitive hydrogel as delivery system for infiltrative local anesthesia:Physico-chemical characterization and pharmacological evaluation[J].Materials Science and Engineering C,2016,68(1):299-307.
[9] 王丽娟,朱照静.泊洛沙姆407温度敏感型原位凝胶的研究进展[J].中国药学杂志,2009,44(4):245-248.
[10] 李学峰,孙媛,胡波,等.高强度水凝胶的摩擦性能研究进展[J].材料导报A,2011,25(1):94-98.
[11] 赵明艳,李立华,周长忍,等.可注射海藻酸钙水凝胶的制备研究[J].功能材料,2010,8(41):1353-1356.
[12] Baysal K,Arouguz A Z,Adiguzel Z,et al.Chitosan/alginate crosslinked hydrogels:preparation,characterization and application for cell growth purposes[J].International Journal of Biological Macromolecules,2013,59(4):342-348.
[13] Loebsack A,Greene K,Wyatt S,et al.In vivo characterization of a porous hydrogel material for use as a tissue bulking agent[J].Journal of Biomedical Materials Research,2001,57(4):575-581.
[14] 李达恒,余学飞,胡云睿,等.壳聚糖-泊洛沙姆为凝胶基质的纳米银抗菌凝胶的制备与表征[J].生物医学工程,2016,33(6):1124-1132.
[15] 胡洁,陈大为,全东琴.泊洛沙姆407水溶液的流变学性质[J].药学学报,2011,46(2):227-231.
[16] 刘让同,李亮.海藻酸钠溶液流变性的浓度依赖性[J].科学技术与工程,2013,13(21):6317-6322.
[2] Muhammad Rizwan,Gary S L Peh,Heng-Pei Ang,et al.Sequentially-crosslinked bioactive hydrogel as nano-patterned substrate with customizable stiffness and degradation for corneal tissue engineering application[J].Biomaterials,2017,120(3):139-154.
[3] 王津,陈莉敏,蒋智清,等.壳聚糖-明胶体系的温敏凝胶及其药物缓释性能[J].功能材料,2013,44(9):1294-1297.
[4] 王波,刁路明.胶原/聚乙烯醇凝胶的制备及生物相容性评价[J].郧阳医学院学报,2010,29(4):335-338.
[5] Friedrich K,Karger-Kocsis J.On the sliding wear performance of polyethernitrile composites[J].Wear,1992,158(1/2):157-170.
[6] Michelle M Blum,Timothy C Ovaert.Investigation of friction and surface degradation of innovative boundary lubricant functionalized hydrogel material for use as artificial articular cartilage[J].Wear,2013,301(1/2):201-209.
[7] 魏宏亮,王连才,张爱英,等.可注射水凝胶的制备与应用[J].化学进展,2004,16(6):1009-1016.
[8] Alessandra C S Akkari,Juliana Z B Papini,Gabriella K Garcia,et al.Poloxamer 407/188 binary thermosensitive hydrogel as delivery system for infiltrative local anesthesia:Physico-chemical characterization and pharmacological evaluation[J].Materials Science and Engineering C,2016,68(1):299-307.
[9] 王丽娟,朱照静.泊洛沙姆407温度敏感型原位凝胶的研究进展[J].中国药学杂志,2009,44(4):245-248.
[10] 李学峰,孙媛,胡波,等.高强度水凝胶的摩擦性能研究进展[J].材料导报A,2011,25(1):94-98.
[11] 赵明艳,李立华,周长忍,等.可注射海藻酸钙水凝胶的制备研究[J].功能材料,2010,8(41):1353-1356.
[12] Baysal K,Arouguz A Z,Adiguzel Z,et al.Chitosan/alginate crosslinked hydrogels:preparation,characterization and application for cell growth purposes[J].International Journal of Biological Macromolecules,2013,59(4):342-348.
[13] Loebsack A,Greene K,Wyatt S,et al.In vivo characterization of a porous hydrogel material for use as a tissue bulking agent[J].Journal of Biomedical Materials Research,2001,57(4):575-581.
[14] 李达恒,余学飞,胡云睿,等.壳聚糖-泊洛沙姆为凝胶基质的纳米银抗菌凝胶的制备与表征[J].生物医学工程,2016,33(6):1124-1132.
[15] 胡洁,陈大为,全东琴.泊洛沙姆407水溶液的流变学性质[J].药学学报,2011,46(2):227-231.
[16] 刘让同,李亮.海藻酸钠溶液流变性的浓度依赖性[J].科学技术与工程,2013,13(21):6317-6322.