发光二极管光质对念珠藻葛仙米生长及生理生化特性的影响
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摘要
利用发光二极管(LED)作为光源,以冷百荧光灯光作为对照,研究不同光质[红光637 nm、绿光529 nm、蓝光453 nm、白光(400—700)nm]对念珠藻葛仙米生长和生理生化特性的影响。结果表明:在培养前期,红光促进藻蓝蛋白合成,而藻红蛋白合成受抑制;蓝光和绿光则促进藻蓝蛋白合成。在培养后期,红光处理有利于叶绿素a和类胡萝卜素积累,其含量分别达到干重的1.33%和0.24%;绿光、白光和冷白荧光培养物的相应色素的含量均约占1.0%和0.16%;蓝光培养物的相应色素含量分别仅为0.45%和0.11%。红光培养物的氨基酸含量达干重的23.1%,是对照的1.58倍。除蓝光外其他光质对还原糖的含量影响无显著差异。在培养过程中LED白光和冷白荧光培养物的平均相对生长速率分别约为其他色光培养物的1.3和1.5倍。
The influence of different Light-Emitting Diode(LED) wavelengths(red 637 nm, green 529 nm, blue 453 nm and white light, set cool white fluorescence as control) on the growth and the physiological and biochemical characteristics of Nostoc sphaeroides Kützing were studied. Results showed that in the initial growth stage, red light promoted the synthesis of phycocyanin and inhibited the phycoerythrin, and the former was also increased by blue and green lights. In the late growth stage, the contents of chlorophyll a and carotenoids in the colonies which cultured under red light accounted for 1.33% and 0.24% of the dry weight, while they were about 1.0% and 0.16% respectively in green, white, or cool white fluorescence cultures, and 0.45% and 0.11% respectively in blue light culture. The content of total amino acids in red light culture was 23.1% in dry weight, which was 1.58 times of the control group. There was no significant difference between the contents of reducing sugars in the cultures under different light wavelengths except blue light. The mean specific growth rates of the white light culture and the cool white fluorescence cultures were about 1.3 and 1.5 times of those in other monochromatic light cultures, respectively.
The influence of different Light-Emitting Diode(LED) wavelengths(red 637 nm, green 529 nm, blue 453 nm and white light, set cool white fluorescence as control) on the growth and the physiological and biochemical characteristics of Nostoc sphaeroides Kützing were studied. Results showed that in the initial growth stage, red light promoted the synthesis of phycocyanin and inhibited the phycoerythrin, and the former was also increased by blue and green lights. In the late growth stage, the contents of chlorophyll a and carotenoids in the colonies which cultured under red light accounted for 1.33% and 0.24% of the dry weight, while they were about 1.0% and 0.16% respectively in green, white, or cool white fluorescence cultures, and 0.45% and 0.11% respectively in blue light culture. The content of total amino acids in red light culture was 23.1% in dry weight, which was 1.58 times of the control group. There was no significant difference between the contents of reducing sugars in the cultures under different light wavelengths except blue light. The mean specific growth rates of the white light culture and the cool white fluorescence cultures were about 1.3 and 1.5 times of those in other monochromatic light cultures, respectively.
引文
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[5]Richmond A.Handbook of Microalgal Culture:Biotechnology and Applied Phycology[M].Oxford,UK:Blackwell Science.2004,125—128
[6]Wolf E,Schussler A.Phycobiliprotein fluorescence of Nostoc punctiforme changes during the life cycle and chromatic adaptation:characterization by spectral confocal laser scanning microscopy and spectral unmixing[J].Plant Cell and Environment,2005,28(4):480—491
[7]Rippka R,Deruelles J,Waterbury J B,et al.Generic assignments,strain histories and properties of pure cultures of cyanobacteria[J].Journal of General Microbiology,1979,111:1—61
[8]Wang Z C,Li D H,Li G B,et al.Mechanism of photosynthetic response in Microcystis aeruginosa PCC7806to low inorganic phosphorus[J].Harmful Algae,2010,9(6):613—619
[9]Wu P P,Rao B Q,Hao Z J,et al.Physiological and ultrastructural characteristics of Scytonema javanicum under high temperature[J].Acta Hydrobiologica Sinica,2012,36(4):735—743[吴沛沛,饶本强,郝宗杰,等.高温培养条件下爪哇伪枝藻的生理特性和超微结构特征.水生生物学报,2012,36(4):735—743]
[10]Li D H.Studies on morphogenesis,development and physiology of Nostoc sphaeroides Kützing(cyanobacterium)[D].Thesis for Doctor of Science,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan.2000[李敦海.拟球状念珠藻(葛仙米)形态建成与发育及其生理学研究.中国科学院水生生物研究所博士学位论文,武汉.2000]
[11]Bennett A,Bogorad L.Complementary chromatic adaptation in a filamentous blue-green-alga[J].Journal of Cell Biology,1973,58(2):419—435
[12]Li H S,Sun Q,Zhao S J.The Experiment Principle and Technology of Plant Physiology[M].Beijing:Higher Education Press.2000,134—137[李合生,孙群,赵世杰.植物生理生化实验原理和技术.高等教育出版社.2000,134—137]
[13]Chinese National Standard Method,The determination of amino acids in the food,GB/T 5009.124—2003[中华人民共和国国家标准,食品中氨基酸的测定,Gb/t 5009.124—2003]
[14]Wolk C P.Physiology and cytological chemistry of bluegreen-algae[J].Bacteriological Reviews,1973,37(1):32—101
[15]Kehoe D M,Grossman A R.Similarity of a chromatic adaptation sensor to phytochrome and ethylene receptors[J].Science,1996,273(5280):1409—1412
[16]Demarsac N T.Occurrence and nature of chromatic adaptation in cyanobacteria[J].Journal of Bacteriology,1977,130(1):82—91
[17]Huai S F,Li X,Cui M M.The development survey of new generation lighting white LED[J].Physics Bulletin,2007,(11):3[怀素芳,李旭,崔敏敏.新一代照明光源白光LED的发展概况.物理通报,2007,(11):3]
[18]Yin C A,Zhao C J,Liu X Y,et al.Development of white light emitting diode[J].Chinese Journal of Luminescence,2000,21(4):3[尹长安,赵成久,刘学彦,等.白光LED的最新进展.发光学报,2000,21(4):3]