遮阴对少叶花楸幼苗光合和叶绿素荧光参数的影响
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摘要
以1年生少叶花楸幼苗为试验材料,采用遮阳网遮光方式设置遮阴度为0、25%、36%、50%、76%、92%6种处理,测定少叶花楸幼苗光合及叶绿素荧光参数,探讨少叶花楸对光照强度的适应。结果表明:在6种处理下,净光合速率和气孔导度日变化均呈单峰曲线,未出现光合"午休现象"。遮阴使少叶花楸幼苗净光合速率、蒸腾速率、气孔导度减小,胞间CO_2摩尔分数增大;叶绿素a质量分数、叶绿素b质量分数和叶绿素总质量分数均随着遮阴度的增加而增大,分别比全光照增加了62.16%、131.37%和83.95%,叶绿素a质量分数/叶绿素b质量分数和类胡萝卜素质量分数随着遮阴度的增加而减小,分别比全光照减小了30.45%和28.57%;遮阴使叶片光能转换效率、有效量子效率、PSⅡ实际光化学效率和光化学淬灭系数增大,最大值分别比全光照增加了6.85%、22%、17.14%和24.59%,非光化学淬灭系数比全光照减小了60.54%。综合表明,适度遮阴诱发了少叶花楸幼苗的光合和生理适应机制。因此,在少叶花楸的引种和栽培过程中,适当遮阴有利于提高其苗木质量。
An experiment was conducted to study the photosynthetic characteristic and chlorophyll fluorescence parameters of one-year Sorbus hupehensis var. paucijuga seedlings under six shade levels( 0,25%,36%,50%,76% and 92%). The diurnal variation of leaf net photosynthetic rate and stomatal conductance of seedling under different shading treatments could be described with a unimodal curve. The net photosynthetic rate,transpiration rate and stomatal conductance were decreased with the decreasing light intensity. With a decrease in light intensity,chlorophyll a,chlorophyll b and total chlorophyll content were increased,which were increased by 62.16%,131.37% and 83.95% when compared to CK,respectively; but chlorophyll a/b and carotenoid contents were decreased,which were reduced by 30.45% and 28.57% compared to CK,respectively; light energy conversion efficiency,effective quantum efficiency,the actual photochemical efficiency of PSⅡ and photochemical quenching were increased with the increase of shading intensity,increased by 6. 85%,22%,17.14% and 24.59% compared to CK,respectively,and non-photochemical quenching coefficient decreased by 60.54%.The results showed that shading stimulated the photosynthetic and physiological adaptation mechanisms in S. hupehensis var. paucijuga seedling,and moderate shading could improve plant quality during the nursery period.
An experiment was conducted to study the photosynthetic characteristic and chlorophyll fluorescence parameters of one-year Sorbus hupehensis var. paucijuga seedlings under six shade levels( 0,25%,36%,50%,76% and 92%). The diurnal variation of leaf net photosynthetic rate and stomatal conductance of seedling under different shading treatments could be described with a unimodal curve. The net photosynthetic rate,transpiration rate and stomatal conductance were decreased with the decreasing light intensity. With a decrease in light intensity,chlorophyll a,chlorophyll b and total chlorophyll content were increased,which were increased by 62.16%,131.37% and 83.95% when compared to CK,respectively; but chlorophyll a/b and carotenoid contents were decreased,which were reduced by 30.45% and 28.57% compared to CK,respectively; light energy conversion efficiency,effective quantum efficiency,the actual photochemical efficiency of PSⅡ and photochemical quenching were increased with the increase of shading intensity,increased by 6. 85%,22%,17.14% and 24.59% compared to CK,respectively,and non-photochemical quenching coefficient decreased by 60.54%.The results showed that shading stimulated the photosynthetic and physiological adaptation mechanisms in S. hupehensis var. paucijuga seedling,and moderate shading could improve plant quality during the nursery period.
引文
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[19] LIU K,TANG C F,ZHOU S B,et al. Comparison of the photosynthetic characteristics of four Lycoris species with leaf appearing in autumn under field conditions[J]. Photosynthetica,2012,50(4):570-576.
[2] WANG Q,HOU F,DONG S,et al. Effects of shading on the photosynthetic capacity,endogenous hormones and root yield in purple-fleshed sweetpotato(Ipomoea batatas(L.)Lam)[J]. Plant Growth Regulation,2014,72(2):113-122.
[3]付忠,谢世清,徐文果,等.不同光照强度下谢君魔芋的光合作用及能量分配特征[J].应用生态学报,2016,27(4):1177-1188.
[4] JUNG J Y,PI J H,PARK J G,et al. Effect of shading levels and soil type on the growth characteristics in potted cultivation of deutzia Paniculata nakai as an endemic plant species in Korea[J].Journal of Agriculture&Life Science,2017,51(2):199-211.
[5]张勇,程怡,王清明,等.遮荫对月季光合特性及生长发育的影响[J].西北植物学报,2014,34(1):162-168.
[6]蔡建国,韦孟琪,章毅,等.遮阴对绣球光合特性和叶绿素荧光参数的影响[J].植物生态学报,2017,41(5):570-576.
[7] YE Z P,SUGGETT D J,ROBAKOWSKI P,et al. A mechanistic model for the photosynthesis-light response based on the photosynthetic electron transport of photosystem II in C3 and C4 species[J]. New Phytologist,2013,199(1):110-120.
[8] YE Z P,ROBAKOWSKI P,SUGGETT D J. A mechanistic model for the light response of photosynthetic electron transport rate based on light harvesting properties of photosynthetic pigment molecules[J]. Planta,2013,237(3):837-847.
[9]张宪政.植物叶绿素含量测定:丙酮乙醇混合液法[J].辽宁农业科学,1986(3):26-28.
[10]陈昕,张红星,张振英.黄山花楸幼苗对遮荫的形态、解剖和光合生理响应[J].东北林业大学学报,2012,40(10):24-27.
[11]罗燕,张永刚,杨利民,等.光照强度对朝鲜淫羊藿生长、光合和叶绿素荧光参数特征的影响[J].东北林业大学学报,2018,46(7):44-48.
[12]赵顺,黄秋娴,李玉灵,等.遮荫处理对臭柏幼苗光合特性的影响[J].生态学报,2014,34(8):1994-2002.
[13]刘柿良,马明东,潘远智,等.不同光环境对桤木幼苗生长和光合特性的影响[J].应用生态学报,2013,24(2):351-358.
[14] SHAO Q S,WANG H Z,GUO H P,et al. Effects of shade treatments on photosynthetic characteristics, chloroplast ultrastructure,and physiology of Anoectochilus roxburghii[J]. PLoS One,2014,9(2).doi:10.1371/journal.pone.0085996.
[15]孙小玲,许岳飞,马鲁沂,等.植株叶片的光合色素构成对遮阴的响应[J].植物生态学报,2010,34(8):989-999.
[16]陈志成,刘晓静,刘畅,等.锐齿栎幼苗生长、光合作用和非结构性碳对遮阴和模拟光斑的响应[J].生态学杂志,2017,36(4):935-943.
[17] ZHOU Y,HUANG L,WEI X,et al. Physiological,morphological,and anatomical changes in Rhododendron agastum in response to shading[J]. Plant Growth Regulation,2017,81(1):23-30.
[18] BAKER N R. Chlorophyll fluorescence:a probe of photosynthesis in vivo[J]. Annual Review of Plant Biology,2008,59(1):89-113.
[19] LIU K,TANG C F,ZHOU S B,et al. Comparison of the photosynthetic characteristics of four Lycoris species with leaf appearing in autumn under field conditions[J]. Photosynthetica,2012,50(4):570-576.