半夏3种不同炮制品中β-谷甾醇含量的比较
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摘要
目的:建立半夏中β-谷甾醇的含量测定方法,比较半夏3种不同炮制品清半夏、法半夏、姜半夏中β-谷甾醇的含量差异。方法:利用紫外可见分光光度法建立,优选测定波长、显色试剂用量、显色时间及显色温度,建立β-谷甾醇的含量测定方法;测定半夏3种不同炮制品中β-谷甾醇的含量。结果:确定了β-谷甾醇显色的条件为检测波长为420 nm,加浓H_2SO_41.0 mL,并于50℃水浴显色4 min,建立β-谷甾醇的含量测定方法,其线性回归方程为y=0.010 1x+0.026 1,R~2=0.999 3,β-谷甾醇在10~70μg/mL浓度范围内与吸光度呈良好线性关系。清半夏、法半夏、姜半夏3种半夏炮制品中β-谷甾醇的含量分别为1.780、1.172、0.844 mg/g。结论:半夏3种炮制品中β-谷甾醇含量清半夏最高,法半夏其次,姜半夏最低。结果可为半夏炮制品的深入研究,尤其是临床上选用半夏组方提供了较好的实验参考依据。
Objective: To establish a method for the determination of β-Sitosterol and compare the content of β-Sitosterol in three kinds of different processed products of Pinellia ternata. Methods: By using ultraviolet-visible spectrophotometry,we established a method for the determination of β-Sitosterol and optimized the measuring wavelength,eagent amount,color time and temperature. Then we determinated the content of β-Sitosterol in Qing Banxia,Fa Banxia and Jiang Banxia. Results:The method for the determination of β-Sitosterol: wavelength 420 nm,mixed H_2SO_4 1. 0 mL,color 4 minutes in 50 ℃ water bath; standard curve regression equation: y = 0. 010 1 x + 0. 026 1,R~2= 0. 999 3. There was a good linear relationship in 10 ~ 70 μg/mL. The contents of β-Sitosterol were 1. 780 mg/g,1. 172 mg/g,0. 844 mg/g respectively in Qing Banxia,Fa Banxia and Jiang Banxia. Conclusion: The content of β-Sitosterol in Qing Banxia is the highest,in Fa Banxia is the second,and that in Jiang Banxia is the minimum. The results could be helpful for the further research of the processed products of Pinellia ternata,providing good experimental references for clinical selection of prescription which containing Pinellia ternata.
Objective: To establish a method for the determination of β-Sitosterol and compare the content of β-Sitosterol in three kinds of different processed products of Pinellia ternata. Methods: By using ultraviolet-visible spectrophotometry,we established a method for the determination of β-Sitosterol and optimized the measuring wavelength,eagent amount,color time and temperature. Then we determinated the content of β-Sitosterol in Qing Banxia,Fa Banxia and Jiang Banxia. Results:The method for the determination of β-Sitosterol: wavelength 420 nm,mixed H_2SO_4 1. 0 mL,color 4 minutes in 50 ℃ water bath; standard curve regression equation: y = 0. 010 1 x + 0. 026 1,R~2= 0. 999 3. There was a good linear relationship in 10 ~ 70 μg/mL. The contents of β-Sitosterol were 1. 780 mg/g,1. 172 mg/g,0. 844 mg/g respectively in Qing Banxia,Fa Banxia and Jiang Banxia. Conclusion: The content of β-Sitosterol in Qing Banxia is the highest,in Fa Banxia is the second,and that in Jiang Banxia is the minimum. The results could be helpful for the further research of the processed products of Pinellia ternata,providing good experimental references for clinical selection of prescription which containing Pinellia ternata.
引文
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[11]陈少芳,黄彦平,万石川.半夏泻心汤含药血清对Hp感染人胃黏膜上皮细胞增殖的影响[J].辽宁中医杂志,2017,44(10):2150-2152.
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[13]刘慧琼,郭书好,沈英森,等.半夏中β-谷甾醇的抗氧化作用研究[J].广东药学院学报,2004,20(3):281-283.
[14]刘倩,陈钧,仰榴青.南沙参中β-谷甾醇的分光光度法测定[J].中国医药工业杂志,2004,35(12):748-750.
[15]陈贞月.论张仲景妙用半夏[J].亚太传统医药,2016,12(3):72-73.
[16]李鹏英,俞年军,李勇.半夏在神经系统疾病中应用的研究进展[J].中国现代中药,2016,18(3):390-395.
[17]刘亚平,邢素芳.半夏的药理作用研究[J].基层医学论坛,2014,32(18):4446-4447.
[18]周华春,周仲霞.半夏炮制前后高效液相图谱变化[J].亚太传统医学,2015,11(8):24-25.
[19]袁海建,贾晓斌,印文静,等.炮制对半夏毒性成分影响及解毒机制研究报道分析[J].中国中药杂志,2016,41(23):4462-4468.
[20]祝之友.半夏的炮制品种与临床性效差异[J].中国中医药现代远程教育,2016,14(15):16.
[21]修艳芹.中药半夏的炮制方法及临床应用[J].世界最新医学信息文摘,2015,83(15):258-259.
[22]周霞,南艳平,万军.半夏中β-谷甾醇的含量测定[J].安徽农业科学,2012,40(3):1424-1425.
[23]Alappat L,Valerio M,Awad A B.Effect of vitamin D andβ-Sitosterol on immune function of macrophages[J].Int Immunopharmacol,2010,10(11):1390-1396.
[2]高景莘,张绿明,卢先明.野生与栽培半夏的镇咳祛痰作用对比研究[J].湖南中医药大学学报,2010,30(7):25-27.
[3]赵永娟,吉中强,张向农,等.生半夏、姜半夏对水貂呕吐作用的影响研究[J].中国中药杂志,2005,34(5):277-279.
[4]徐宁,王莉,牛争平,等.半夏、钩藤乙醇提取物4:1配伍对青霉素诱发惊厥大鼠痫性放电的影响[J].中西医结合心脑血管病杂志,2010,8(3):322-324.
[5]赵江丽,赵婷,张敏,等.半夏不同溶剂提取物镇静催眠活性比较[J].安徽农业科学,2011,39,(35):21627-21628.
[6]曾颂,李书渊,吴志坚,等.半夏镇咳祛痰的成分-效应关系研究[J].中国现代中药,2013(6):452-455.
[7]卢红阳,蔡菊芬,杜灵彬,等.半夏泻心汤对伊立替康致小细胞肺癌荷瘤鼠胃损伤的改善作用[J].中华中医药学刊,2012,30(6):1289-1290.
[8]王震,姜月华,吴赛,等.半夏白术天麻汤改善肥胖性高血压大鼠肾脏损害的机制研究[J].中华中医药学刊,2016,34(9):2130-2133.
[9]吴宏华,周先富.H型高血压患者采用半夏白术天麻汤加减综合治疗对改善血压及血浆Hcy的影响[J].中华中医药学刊,2016,34(9):2295-2297.
[10]倪瑶,吕文良,李娟梅,等.半夏泻心汤治疗消化系统疾病作用机制研究进展[J].辽宁中医药大学学报,2017,19(9):89.
[11]陈少芳,黄彦平,万石川.半夏泻心汤含药血清对Hp感染人胃黏膜上皮细胞增殖的影响[J].辽宁中医杂志,2017,44(10):2150-2152.
[12]张之昊,戴忠,胡晓茹,等.半夏化学成分的分离与鉴定[J].中药材,2013,36(10):1620-1622.
[13]刘慧琼,郭书好,沈英森,等.半夏中β-谷甾醇的抗氧化作用研究[J].广东药学院学报,2004,20(3):281-283.
[14]刘倩,陈钧,仰榴青.南沙参中β-谷甾醇的分光光度法测定[J].中国医药工业杂志,2004,35(12):748-750.
[15]陈贞月.论张仲景妙用半夏[J].亚太传统医药,2016,12(3):72-73.
[16]李鹏英,俞年军,李勇.半夏在神经系统疾病中应用的研究进展[J].中国现代中药,2016,18(3):390-395.
[17]刘亚平,邢素芳.半夏的药理作用研究[J].基层医学论坛,2014,32(18):4446-4447.
[18]周华春,周仲霞.半夏炮制前后高效液相图谱变化[J].亚太传统医学,2015,11(8):24-25.
[19]袁海建,贾晓斌,印文静,等.炮制对半夏毒性成分影响及解毒机制研究报道分析[J].中国中药杂志,2016,41(23):4462-4468.
[20]祝之友.半夏的炮制品种与临床性效差异[J].中国中医药现代远程教育,2016,14(15):16.
[21]修艳芹.中药半夏的炮制方法及临床应用[J].世界最新医学信息文摘,2015,83(15):258-259.
[22]周霞,南艳平,万军.半夏中β-谷甾醇的含量测定[J].安徽农业科学,2012,40(3):1424-1425.
[23]Alappat L,Valerio M,Awad A B.Effect of vitamin D andβ-Sitosterol on immune function of macrophages[J].Int Immunopharmacol,2010,10(11):1390-1396.