青岛海泊河污水处理厂缺氧池中微生物群落动态变化
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摘要
通过高通量测序考察了不同月份青岛海泊河污水处理厂缺氧池中微生物群落的动态变化。研究结果表明,在8和10月缺氧池中微生物群落丰度较高,1月缺氧池污泥中微生物群落丰度较小。缺氧池微生物群落多样性在12月较高,在6月微生物群落多样性较小。不同月份缺氧池中共有分类操作单元(OTU)中占优势地位的门包括Proteobacteria(42.3%)、Bacteroidetes(21.0%)、Chloroflexi(6.5%)、Firmicutes(6.3%)、Actinobacteria(4.0%)和Planctomycetes(3.8%),大部分微生物一直存在于缺氧池中。污泥样品中微生物群落的主成分分析和主坐标分析表明,8和10月缺氧池中微生物群落比较相似,但与1月污泥样品中的微生物群落之间存在着明显的差异。进水水质和环境温度的变化导致在门、纲和属水平上缺氧池内微生物的种类和相对丰度在不同月份呈现明显的变化。
The dynamic variations of microbial community in the anoxic tank from Haibo river wastewater treatment plant of Qindao was investigated at different months by high-throughput sequencing.The microbial richness of the anoxic tank was higher at August and October and lower at January.The microbial diversity of the anoxic tank was higher at December and lower at June.The predominant phyla of common operational taxonomic units(OTU)in the anoxic tanks at different months mainly consisted of Proteobacteria(42.3%),Bacteroidetes(21.0%),Chloroflexi(6.5%),Firmicutes(6.3%),Actinobacteria(4.0%)and Planctomycetes(3.8%),suggesting that most microorganism always appeared during the operational process.The analysis of principal component and principal coordinate's ordination showed that the microbial community of activated sludge in the anoxic tank at August was similar to that at October,whereas there was obvious difference in the microbial community of activated sludge among August,October and January.The microbial richness and microbial diversity of the anoxic tank had obvious changes at different months at the phyla,class and genus levels due to the variations of the influent temperature and compositions.
The dynamic variations of microbial community in the anoxic tank from Haibo river wastewater treatment plant of Qindao was investigated at different months by high-throughput sequencing.The microbial richness of the anoxic tank was higher at August and October and lower at January.The microbial diversity of the anoxic tank was higher at December and lower at June.The predominant phyla of common operational taxonomic units(OTU)in the anoxic tanks at different months mainly consisted of Proteobacteria(42.3%),Bacteroidetes(21.0%),Chloroflexi(6.5%),Firmicutes(6.3%),Actinobacteria(4.0%)and Planctomycetes(3.8%),suggesting that most microorganism always appeared during the operational process.The analysis of principal component and principal coordinate's ordination showed that the microbial community of activated sludge in the anoxic tank at August was similar to that at October,whereas there was obvious difference in the microbial community of activated sludge among August,October and January.The microbial richness and microbial diversity of the anoxic tank had obvious changes at different months at the phyla,class and genus levels due to the variations of the influent temperature and compositions.
引文
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[2]Shu D T,He Y L,Yue H,et al.Metagenomic and quantitative insights into microbial communities and functional genes of nitrogen and iron cycling in twelve wastewater treatment systems[J].Chemical Engineering Journal,2016,290:21-30.
[3]方芳,秦宇,郭劲松,等.单级自养脱氮反应器效能与微生物群落结构的相关性[J].土木建筑与环境工程,2010,32(3):113-117.Fang F,Qin Y,Guo J S,et al.Relationship between performance and microbial community structure of the SBBR autotrophic nitrogen removal process[J].Journal of Civil,Architecture and Environmental Engineering,2010,32(3):113-117.
[4]徐少娟,蒋涛,殷峻,等.进水氨氮浓度对强化生物除磷(EBPR)系统除磷特性及微生物群落结构的影响[J].环境科学学报,2011,31(4):745-751.Xu S J,Jiang T,Yin J,et al.Effects of influent ammonia concentration on phosphorus removal and the microbial community in an EBPR system[J].Acta Science Circumstantiae,2011,31(4):745-751.
[5]Amann R I,Ludwig W,Schleifer K H.Phylogenetic identification and in situ detection of individual microbial cells without cultivation[J].Microbiology Review,1995,59:143-169.
[6]王浩.高通量测序等分子生物学技术研究填埋场生物反应器脱氮微生物群落变化[D].上海:华东师范大学,2014.Wang Hao.Study on Microbial Community Structure of Nitrogen Removal Process in Land Fill Bioreactor by Using High-Throughput Sequencing and Other Molecular Biological Techniques[D].Shanghai:East China Normal University,2014.
[7]闫媛,黎力,王亚宜,等.采用高通量测序分析全程自养脱氮(CANON)系统不同脱氮效能下的微生物群落结构[J].北京工业大学学报,2015,41(10):1485-1492.Yan Y,Li K,Wang Y Y,et al.Microbial community characteristics of a completely autotrophic nitrogen removal over nitrite(CANON)system based on high-throughput sequencing technology[J].Journal of Beijing University of Technology,2015,41(10):1485-1492.
[8]Ma B R,Wang S,Li Z W,et al.Magnetic Fe3O4nanoparticles induced effects on performance and microbial community of activated sludge from a sequencing batch reactor under long-term exposure[J].Bioresource Technology,2016,225:377-385.
[9]Wang S,Gao M C,She Z L,et al.Long-term effects of ZnO nanoparticles on nitrogen and phosphorus removal,microbial activity and microbial community of a sequencing batch reactor[J].Bioresource Technology,2016,216:428-436.
[10]Gao C Y,Wang A J,Wu W M,et al.Enrichment of anodic biofilm inoculated with anaerobic or aerobic sludge in single chambered air-cathode microbial fuel cells[J].Bioresource Technology,2014,167:124-132.
[11]Liu W R,Yang D H,Chen W J,et al.High-throughput sequencing-based microbial characterization of size fractionated biomass in an anoxic anammox reactor for low-strength wastewater at low temperatures[J].Bioresource Technology,2017,231:45-52.
[12]Ma Q,Qu Y Y,Shen W L,et al.Bacterial community compositions of coking wastewater treatment plants in steel industry revealed by Illumina high-throughput sequencing[J].Bioresource Technology,2015,179:436-443.
[13]Liu Z,Zhang C,Wang L,et al.Effects of furan derivatives on biohydrogen fermentation from wet stream-exploded cornstalk and its microbial community[J].Bioresource Technology,2015,175:152-159.
[14]Li C,Zhang Z,Cao J S,et al.Study on poultry manure wastewater treatment by two-stage aerobic coupled process and its microbial community analysis[J].Biochemical Engineering Journal,2016,110:101-111.
[15]Yoshifumi S,Yasuyoshi S,Hiroshi U,et al.Aerobic and anaerobic toluene degradation by a newly isolated denitrifying bacterium,Thauerasp.strain DNT-1[J].Applied and Environmental Microbiology,2004,70(3):1385-1392.
[16]Scholten E,Auling G R M,Rainey F A,et al.Thauera mechernichensis sp.nov.,an aerobic denitrifier from a leachate treatment plant[J].International Journal of Systematic Bacteriology,1999,49(3):1045-1051.
[17]Horn M A,Julian I,Carola M,et al.Dechloromonas denitrificans sp.nov.,Flavobacterium denitrificans sp.nov.,Paenibacillus anaericanus sp.nov.and Paenibacillus terrae strain MH72,N2O-producing bacteria isolated from the gut of the earthworm Aporrectodea caliginosa[J].International Journal of Systematic and Evolutionary Microbiology,2005,55(3):1255-1265
[18]Watanabe T,Kojima H,Fukui M,et al.Complete genomes of freshwater sulfur oxidizers Sulfuricella denitrificans skB26and Sulfuritalea hydrogenivorans sk43H:Genetic insights into the sulfur oxidation pathway of betaproteo bacteria[J].Systematic and Applied Microbiology,2014,37(6):387-395
[19]Christiane G D,Michael P,Katharina K,et al.Functionally relevant diversity of closely related Nitrospira in activated sludge[J].Journal of Microbial Ecology,2015,9(3):643-655
[20]Kim J M,Lee H J,Lee D S,et al.Characterization of the denitrification-associated phosphorus uptake properties“Candidatus Accumulibacter phosphatis”clades in sludge subjected to enhanced biological phosphorus removal[J].Applied Environmental Microbiology,2013,79(6):1969-1979.