大鼠挤压综合征模型下姜黄素的干预效果研究
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摘要
目的:探讨在大鼠挤压综合征模型下,姜黄素的干预效果研究。方法:利用人工制备的大鼠挤压模型,测量在不同的挤压时间下(6、12、24 h)以及在不同剂量[普通剂量组(100 mg/kg)组及高剂量组(200 mg/kg)]姜黄素干预大鼠挤压综合征模型的情况下,尿素氮、肌酐、钾离子的水平,观察挤压综合征的两大特点:肾功能损伤及高钾血症相应情况。结果:对照组、挤压组、姜黄素普通剂量组(100 mg/kg)和姜黄素高剂量组(200 mg/kg)的数据几乎都出现了一个统一的现象,在尿素氮、肌酐、钾离子3个项目上,均是在时间属性上的数据出现了统计学差异,并且任意两个数据之间都出现统计学差异(P<0.05);在组别的属性上,4个组别也出现了统计学差异,且任意两个数据之间也都出现统计学差异(P<0.05),在尿素氮、肌酐、血钾3个项目上,以姜黄素用量和不同时间点的对照组、挤压组、姜黄素普通剂量组(100 mg/kg)和姜黄素高剂量组(200 mg/kg)的数据的spearman秩相关分析,可以看出在3个项目的每个时间点的纵向数据中,除出现了P<0.05的情况,并且r值均小于零。结论:姜黄素在大鼠挤压综合征模型中,可以有效的缓解肾功能的损伤,作为一种常见而且廉价的中药制剂,在挤压综合征的治疗过程中,值得向更大的范围推广。
Objective: To investigate the effect of curcumin on rat model of crush syndrome. Methods: The crush syndrome model was set up. Measure the levels of blood urea nitrogen,creatinine and potassium ion in rats with crush syndrome model under different doses( 200 mg/kg and 100 mg/kg) in different extrusion time( 6 h,12 h,24 h). Two characteristics of crush syndrome were observed: renal function injury and corresponding hyperkalemia. Result: The data of control group,extrusion group,curcumin general dose group( 100 mg/kg) and curcumin high dose group( 200 mg/kg) almost all showed a unified phenomenon in urea nitrogen,creatinine and potassium ion three projects. There were statistically significant differences in the data of the time attributes and there was statistical difference between any two data( P<0. 05). The four groups also appeared significance in statistics( P<0. 05). In the three items of urea nitrogen,creatinine and serum potassium,the curcumin dosage and different time points of the control group,the extrusion group,the curcumin group and the control group were significantly different( P<0. 05). Spearman rank correlation analysis of the data of the curcumin general dose group( 100 mg/kg) and the high dose group of curcumin( 200 mg/kg) showed that in the longitudinal data of each time point of the three items was significant,P<0. 05 and the r value was less than zero. Conclusion: Curcumin in the rat model of crush syndrome can effectively alleviate the damage of renal function. As a common and cheap traditional Chinese medicine in the treatment of crush syndrome,it is worth to a wider range of promotion.
Objective: To investigate the effect of curcumin on rat model of crush syndrome. Methods: The crush syndrome model was set up. Measure the levels of blood urea nitrogen,creatinine and potassium ion in rats with crush syndrome model under different doses( 200 mg/kg and 100 mg/kg) in different extrusion time( 6 h,12 h,24 h). Two characteristics of crush syndrome were observed: renal function injury and corresponding hyperkalemia. Result: The data of control group,extrusion group,curcumin general dose group( 100 mg/kg) and curcumin high dose group( 200 mg/kg) almost all showed a unified phenomenon in urea nitrogen,creatinine and potassium ion three projects. There were statistically significant differences in the data of the time attributes and there was statistical difference between any two data( P<0. 05). The four groups also appeared significance in statistics( P<0. 05). In the three items of urea nitrogen,creatinine and serum potassium,the curcumin dosage and different time points of the control group,the extrusion group,the curcumin group and the control group were significantly different( P<0. 05). Spearman rank correlation analysis of the data of the curcumin general dose group( 100 mg/kg) and the high dose group of curcumin( 200 mg/kg) showed that in the longitudinal data of each time point of the three items was significant,P<0. 05 and the r value was less than zero. Conclusion: Curcumin in the rat model of crush syndrome can effectively alleviate the damage of renal function. As a common and cheap traditional Chinese medicine in the treatment of crush syndrome,it is worth to a wider range of promotion.
引文
[1]黄真真,曾瑜,贾贞超,等.姜黄素及其衍生物的抗氧化作用研究进展[J].预防医学情报杂志,2016,32(11):1237-1240.
[2]李鸿鹏,徐兆发,郭美欣,等.姜黄素对汞致大鼠肾损伤保护作用的实验研究[J].毒理学杂志,2016,30(3):233-236.
[3]於四军,严政,路靖,等.早期大剂量地塞米松对挤压综合征大鼠模型心肌特异性损伤的疗效[J].武警医学,2016,27(3):281-288.
[4]唐怡,张凌,杨莹莹,等.连续性静脉-静脉血液滤过治疗山羊挤压综合征模型的建立[J].四川大学学报(医学版),2016,47(1):28-32.
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[6]李爽,陶艳艳,刘成海.肝硬化急性肾损伤诊断标准研究进展[J].中国实用内科杂志,2015,35(12):1044-1046.
[7]刘涵,苟亚军,彭东,等.PE联合CVVH治疗挤压综合征患者临床疗效研究[J].临床急诊杂志,2015,16(8):585-587.
[8]刘同存,许硕葵,乔变莲,等.CRRT技术在创伤性挤压综合征中的应用与护理[J].西北国防医学杂志,2015,36(7):482-484.
[9]谢光亮,朱琳,张燕敏,等.铁代谢在大鼠缺血再灌注急性肾损伤后的变化[J].肾脏病与透析肾移植杂志,2016,25(5):444-449.
[10]郭美欣,徐兆发,李鸿鹏,等.莱菔硫烷对汞致大鼠肾Nrf2、HO-1、γ-GCS、Gpx-1的mRNA和蛋白表达影响[J].中国工业医学杂志,2016,29(5):365-379.
[11]张静,张艳,孙明慧,等.Ig A大鼠肾组织中TGF-β1的表达与肾损害关联性研究[J].中国中西医结合肾病杂志,2016,17(10):854-857.
[12]冯金环,王孟兰,张俊英,等.支气管哮喘儿童血清s Fsa、s Fas L的临床意义[J].中国儿童保健杂志,2016,24(8):887-891.
[13]肖瑛,刘瑞霞,石明隽,等.PKCα参与高糖调节大鼠肾小管上皮细胞骨形态发生蛋白-7的表达[J].基础医学与临床,2016,17(8):800-804.
[14]魏升,蔡旭东,钟光辉.复方黄芪当归汤对MS大鼠肾损害的干预作用及其机制研究[J].中华中医药学刊,2016,17(8):811-814.
[15]胡爽,王美.糖肾安对高糖环境下大鼠肾脏及肾系膜细胞炎性因子ICAM-1表达的影响[J].现代中西医结合杂志,2016,13(6):992-996.
[2]李鸿鹏,徐兆发,郭美欣,等.姜黄素对汞致大鼠肾损伤保护作用的实验研究[J].毒理学杂志,2016,30(3):233-236.
[3]於四军,严政,路靖,等.早期大剂量地塞米松对挤压综合征大鼠模型心肌特异性损伤的疗效[J].武警医学,2016,27(3):281-288.
[4]唐怡,张凌,杨莹莹,等.连续性静脉-静脉血液滤过治疗山羊挤压综合征模型的建立[J].四川大学学报(医学版),2016,47(1):28-32.
[5]陈怀祥,田海平,刘学军.51例挤压伤院前急救分析[J].华西医学,2016,31(1):53-56.
[6]李爽,陶艳艳,刘成海.肝硬化急性肾损伤诊断标准研究进展[J].中国实用内科杂志,2015,35(12):1044-1046.
[7]刘涵,苟亚军,彭东,等.PE联合CVVH治疗挤压综合征患者临床疗效研究[J].临床急诊杂志,2015,16(8):585-587.
[8]刘同存,许硕葵,乔变莲,等.CRRT技术在创伤性挤压综合征中的应用与护理[J].西北国防医学杂志,2015,36(7):482-484.
[9]谢光亮,朱琳,张燕敏,等.铁代谢在大鼠缺血再灌注急性肾损伤后的变化[J].肾脏病与透析肾移植杂志,2016,25(5):444-449.
[10]郭美欣,徐兆发,李鸿鹏,等.莱菔硫烷对汞致大鼠肾Nrf2、HO-1、γ-GCS、Gpx-1的mRNA和蛋白表达影响[J].中国工业医学杂志,2016,29(5):365-379.
[11]张静,张艳,孙明慧,等.Ig A大鼠肾组织中TGF-β1的表达与肾损害关联性研究[J].中国中西医结合肾病杂志,2016,17(10):854-857.
[12]冯金环,王孟兰,张俊英,等.支气管哮喘儿童血清s Fsa、s Fas L的临床意义[J].中国儿童保健杂志,2016,24(8):887-891.
[13]肖瑛,刘瑞霞,石明隽,等.PKCα参与高糖调节大鼠肾小管上皮细胞骨形态发生蛋白-7的表达[J].基础医学与临床,2016,17(8):800-804.
[14]魏升,蔡旭东,钟光辉.复方黄芪当归汤对MS大鼠肾损害的干预作用及其机制研究[J].中华中医药学刊,2016,17(8):811-814.
[15]胡爽,王美.糖肾安对高糖环境下大鼠肾脏及肾系膜细胞炎性因子ICAM-1表达的影响[J].现代中西医结合杂志,2016,13(6):992-996.