玉米组合M9916×D472抗镰孢茎腐病的六世代联合数量遗传研究
|
摘要
以玉米自交系M9916×D472组成的六世代群体为材料,利用六世代数量性状主基因+多基因混合遗传模型多世代联合分析方法,研究玉米茎腐病抗性的遗传机制。结果表明,分离世代的次数频率分布在F2群体中呈近似于抛物线状的正态分布,在B1和B2群体中呈偏正态分布。通过AIC值适合性检验,该性状符合1对加-显主基因+加-显-上位性多基因遗传D模型,主基因加性效应值为5.29,显性效应值为6.79,两者效应值均相对较高,加性与显性效应比率为78%;多基因加性效应值为2.98,明显低于显性效应值6.88,说明显性效应起主导作用。多基因加性互作效应明显,加、显互作上存在一定抑制作用,显性互作效应不明显,加性互作效应在上位性方面起主导作用。回交群体中主基因的遗传率介于39.9%~41.3%,多基因遗传率介于33.8%~36.7%,主基因遗传率相对较高;自交群体中多基因遗传率为53.8%,大于主基因遗传率32.8%,说明在进代过程中主基因遗传率不断下降。在抗茎腐病的育种中,应注重早代抗病材料的选择,对现有重要的感病材料可利用回交方法进行有效改良。
Six generations of maize inbred lines of M9916×D472 were selected as the research materials tostudy the inheritance of maize stalk rot by the mixed major gene and polygene inheritance model. The resultsshowed that, the frequency distribution in F2 population was similar to normal distribution, a skewed normal distribu-tion in B1 and B2 populations. Maize stalk rot resistance trait fitted the model of a pair of additive-dominance majorgene plus additive-dominance-epitasis polygene(D Model) through the group AIC value fit test. The major gene ad-ditive effect value was 5.29, dominant effect value was 6.79, and their rate was 78%. The polygene additive effectwas 2.98, it was lower than the dominant effect value(6.88) who played the important role. The polygene additive ge-netic interaction effect was obviously, the dominant interaction effect was just the opposite, it has certain inhibitoryon additive-dominant interaction effect, and additive interactions effect played a leading role in epitasis. The majorgene heritability was between 39.9% and 41.3%, the polygene heritability was between 33.8% and 36.7% in back-cross populations. The major gene heritability was 32.8%, it was lower than polygene heritability(53.8%) in selfingpopulations, and the major gene heritability was falling on selfing process. It should be pay more attention to selectearly generation of disease-resistant material or put backcross breeding to improve the breeding value.
Six generations of maize inbred lines of M9916×D472 were selected as the research materials tostudy the inheritance of maize stalk rot by the mixed major gene and polygene inheritance model. The resultsshowed that, the frequency distribution in F2 population was similar to normal distribution, a skewed normal distribu-tion in B1 and B2 populations. Maize stalk rot resistance trait fitted the model of a pair of additive-dominance majorgene plus additive-dominance-epitasis polygene(D Model) through the group AIC value fit test. The major gene ad-ditive effect value was 5.29, dominant effect value was 6.79, and their rate was 78%. The polygene additive effectwas 2.98, it was lower than the dominant effect value(6.88) who played the important role. The polygene additive ge-netic interaction effect was obviously, the dominant interaction effect was just the opposite, it has certain inhibitoryon additive-dominant interaction effect, and additive interactions effect played a leading role in epitasis. The majorgene heritability was between 39.9% and 41.3%, the polygene heritability was between 33.8% and 36.7% in back-cross populations. The major gene heritability was 32.8%, it was lower than polygene heritability(53.8%) in selfingpopulations, and the major gene heritability was falling on selfing process. It should be pay more attention to selectearly generation of disease-resistant material or put backcross breeding to improve the breeding value.
引文
[1]吴海燕,孙淑荣,范作伟,等.玉米茎腐病研究现状与防治对策[J].玉米科学,2007,15(4):129-132.Wu H Y,Sun S R,Fan Z W,et al.Research condition and prevention countermeasures of maize stalk rot[J].Journal of Maize Sciences,2007,15(4):129-132.(in Chinese)
[2]段灿星,王晓鸣,武小菲,等.玉米种质和新品种对腐霉茎腐病和镰孢穗腐病的抗性分析[J].植物遗传资源学报,2015,16(5):947-954.Duan C X,Wang X M,Wu X F,et al.Analysis of maize accessions resistance to pythium stalk rot and Fusarium ear rot[J].Journal of Plant Genetic Resources,2015,16(5):947-954.(in Chinese)
[3]范志业,崔小伟,施艳,等.河南省玉米茎基腐病主要病原菌鉴定及主栽玉米品种的抗性分析[J].河南农业科学,2014,,43(12):87-90.Fan Z Y,Cui X W,Shi Y,et al.Identification of pathogens causing corn stalk rot and resistance test of maize cultivars in Henan provincet[J].Journal of Henan Agricultural Sciences,2014,43(12):87-90.(in Chinese)
[4]王良发,徐国举,张守林,等.对25个玉米品种的茎腐病抗性分析和产量损失评估[J].玉米科学,2015,23(6):12-17.Wang L F,Xu G J,Zhang S L,et al.Analysis of the resistance to stalk rot and yield loss evaluation of 25 maize varieties[J].Journal of Maize Sciences,2015,23(6):12-17.(in Chinese)
[5]魏昕,李丽华,王娟,等.玉米丝裂病发生的数量遗传分析[J].中国农业科学,2008,41(8):2235-2240.Wei X,Li L H,Wang J,et al.Quantitative genetic analysis of silk cut in maize(Zea mays L.)[J].Scientia Agricultura Sinica,2008,41(8):2235-2240.(in Chinese)
[6]盖钧镒,章元明,王建康.植物数量性状遗传体系[M].北京:科学出版社,2003.
[7]鞠方成.玉米雌穗一节多穗性状的遗传研究[J].玉米科学,2015,23(6):7-11.Ju F C.Genetics study of maize one internode more ears traits[J].Journal of Maize Sciences,2015,23(6):7-11.(in Chinese)
[8]李红霞,赵仁贵,戴冬青,等.甜玉米含糖量性状的遗传研究[J].吉林农业科学,2014,39(1):30-32,36.Li H X,Zhao R G,Dai D Q,et al.Studies on genetic of sugar content of sweet corn[J].Journal of Jilin Agricultural Sciences,2014,39(1):30-32,36.(in Chinese)
[9]丰光,景希强,李妍妍,等.玉米穗上叶与主茎夹角性状的数量遗传研究[J].玉米科学,2012,20(1):53-56,66.Feng G,Jing X Q,Li Y Y,et al.Quantitative genetic study of the leaf angle uppermost ear traits in maize(Zea mays L.)[J].Journal of Maize Sciences,2012,20(1):53-56,66.(in Chinese)
[10]李妍妍,景希强,丰光,等.玉米茎秆拉力强度相关的倒伏遗传研究[J].玉米科学,2013,21(4):20-23.Li Y Y,Jing X Q,Feng G,et al.Genetics of lodging resistance in tolerance to maize stem pull strength[J].Journal of Maize Sciences,2013,21(4):20-23.(in Chinese)
[11]马飞前,刘小刚,王红武,等.玉米茎秆纤维素含量遗传分析[J].玉米科学,2015,23(1):10-16.Ma F Q,Liu X G,Wang H W,et al.Genetic study on cellulose content of stalk in maize[J].Journal of Maize Sciences,2015,23(1):10-16.(in Chinese)
[12]陈楠,潘晓静,姚远,等.东北地区玉米茎腐病镰孢菌EF-1α基因序列分析鉴定[J].玉米科学,2015,23(4):143-148.Chen N,Pan X J,Yao Y,et al.Sequence analysis of EF-1αgene in Fusarium species causing the maize stalk rot in northeast China[J].Journal of Maize Sciences,2015,23(4):143-148.(in Chinese)
[13]程玉静,陈国清,薛林,等.玉米茎腐病研究进展[J].安徽农业科学,2013,41(20):8557-8559,8569.Cheng Y J,Chen G Q,Xue L,et al.Research advance of maize stalk rot[J].Journal of Anhui Agricultural Sciences,2013,41(20):8557-8559,8569.(in Chinese)
[14]Xu S F,Chen J,Gao Z G,et al.Maize stalk rot and ear rot in China[J].Acta Phytopathologica Sinica,2006,36(3):193-203.
[15]孔晓民,韩成卫,宋春林,等.不同营养元素对玉米茎腐病的调控效果[J].河南农业科学,2016,45(9):69-72.Kong X M,Han C W,Song C L,et al.Control effect of different nutrient elements on maize stalk rot[J].Journal of Henan Agricultural Sciences,2016,45(9):69-72.(in Chinese)
[16]宋燕春,裴二芹,石云素,等.玉米重要自交系的肿囊腐霉茎腐病抗性鉴定与评价[J].植物遗传资源学报,2012,13(5):798-802.Song Y C,Pei E Q,Shi Y S,et al.Identification and evaluation of resistance to stalk rot(Pythium inflatum matthews)in important inbred lines of maize[J].Journal of Plant Genetic Resources,2012,13(5):798-802.(in Chinese)
[17]贾曦,王璐,刘振林,等.玉米杂交种和自交系抗茎腐病鉴定[J].山东农业科学,2017,49(1):114-116.Jia X,Wang L,Liu Z L,et al.Resistance identification of maize hybrids and inbred lines to stalk rot[J].Shandong Agricultural Sciences,2017,49(1):114-116.(in Chinese)
[18]杨双,韩晓日.玉米早衰相关性状的遗传分析[J].玉米科学,2015,23(3):1-7,14.Yang S,Han X R.Genetic analysis of premature senescence related traits in maize[J].Journal of Maize Sciences,2015,23(3):1-7,14.(in Chinese)
[19]郑洪建,孔令杰,许瑞瑞,等.玉米叶片保绿性数量遗传分析[J].玉米科学,2013,21(6):17-20.Zheng H J,Kong L J,Xu R R,et al.Genetic analysis of stay-green traits in maize[J].Journal of Maize Sciences,2013,21(6):17-20.(in Chinese)
[2]段灿星,王晓鸣,武小菲,等.玉米种质和新品种对腐霉茎腐病和镰孢穗腐病的抗性分析[J].植物遗传资源学报,2015,16(5):947-954.Duan C X,Wang X M,Wu X F,et al.Analysis of maize accessions resistance to pythium stalk rot and Fusarium ear rot[J].Journal of Plant Genetic Resources,2015,16(5):947-954.(in Chinese)
[3]范志业,崔小伟,施艳,等.河南省玉米茎基腐病主要病原菌鉴定及主栽玉米品种的抗性分析[J].河南农业科学,2014,,43(12):87-90.Fan Z Y,Cui X W,Shi Y,et al.Identification of pathogens causing corn stalk rot and resistance test of maize cultivars in Henan provincet[J].Journal of Henan Agricultural Sciences,2014,43(12):87-90.(in Chinese)
[4]王良发,徐国举,张守林,等.对25个玉米品种的茎腐病抗性分析和产量损失评估[J].玉米科学,2015,23(6):12-17.Wang L F,Xu G J,Zhang S L,et al.Analysis of the resistance to stalk rot and yield loss evaluation of 25 maize varieties[J].Journal of Maize Sciences,2015,23(6):12-17.(in Chinese)
[5]魏昕,李丽华,王娟,等.玉米丝裂病发生的数量遗传分析[J].中国农业科学,2008,41(8):2235-2240.Wei X,Li L H,Wang J,et al.Quantitative genetic analysis of silk cut in maize(Zea mays L.)[J].Scientia Agricultura Sinica,2008,41(8):2235-2240.(in Chinese)
[6]盖钧镒,章元明,王建康.植物数量性状遗传体系[M].北京:科学出版社,2003.
[7]鞠方成.玉米雌穗一节多穗性状的遗传研究[J].玉米科学,2015,23(6):7-11.Ju F C.Genetics study of maize one internode more ears traits[J].Journal of Maize Sciences,2015,23(6):7-11.(in Chinese)
[8]李红霞,赵仁贵,戴冬青,等.甜玉米含糖量性状的遗传研究[J].吉林农业科学,2014,39(1):30-32,36.Li H X,Zhao R G,Dai D Q,et al.Studies on genetic of sugar content of sweet corn[J].Journal of Jilin Agricultural Sciences,2014,39(1):30-32,36.(in Chinese)
[9]丰光,景希强,李妍妍,等.玉米穗上叶与主茎夹角性状的数量遗传研究[J].玉米科学,2012,20(1):53-56,66.Feng G,Jing X Q,Li Y Y,et al.Quantitative genetic study of the leaf angle uppermost ear traits in maize(Zea mays L.)[J].Journal of Maize Sciences,2012,20(1):53-56,66.(in Chinese)
[10]李妍妍,景希强,丰光,等.玉米茎秆拉力强度相关的倒伏遗传研究[J].玉米科学,2013,21(4):20-23.Li Y Y,Jing X Q,Feng G,et al.Genetics of lodging resistance in tolerance to maize stem pull strength[J].Journal of Maize Sciences,2013,21(4):20-23.(in Chinese)
[11]马飞前,刘小刚,王红武,等.玉米茎秆纤维素含量遗传分析[J].玉米科学,2015,23(1):10-16.Ma F Q,Liu X G,Wang H W,et al.Genetic study on cellulose content of stalk in maize[J].Journal of Maize Sciences,2015,23(1):10-16.(in Chinese)
[12]陈楠,潘晓静,姚远,等.东北地区玉米茎腐病镰孢菌EF-1α基因序列分析鉴定[J].玉米科学,2015,23(4):143-148.Chen N,Pan X J,Yao Y,et al.Sequence analysis of EF-1αgene in Fusarium species causing the maize stalk rot in northeast China[J].Journal of Maize Sciences,2015,23(4):143-148.(in Chinese)
[13]程玉静,陈国清,薛林,等.玉米茎腐病研究进展[J].安徽农业科学,2013,41(20):8557-8559,8569.Cheng Y J,Chen G Q,Xue L,et al.Research advance of maize stalk rot[J].Journal of Anhui Agricultural Sciences,2013,41(20):8557-8559,8569.(in Chinese)
[14]Xu S F,Chen J,Gao Z G,et al.Maize stalk rot and ear rot in China[J].Acta Phytopathologica Sinica,2006,36(3):193-203.
[15]孔晓民,韩成卫,宋春林,等.不同营养元素对玉米茎腐病的调控效果[J].河南农业科学,2016,45(9):69-72.Kong X M,Han C W,Song C L,et al.Control effect of different nutrient elements on maize stalk rot[J].Journal of Henan Agricultural Sciences,2016,45(9):69-72.(in Chinese)
[16]宋燕春,裴二芹,石云素,等.玉米重要自交系的肿囊腐霉茎腐病抗性鉴定与评价[J].植物遗传资源学报,2012,13(5):798-802.Song Y C,Pei E Q,Shi Y S,et al.Identification and evaluation of resistance to stalk rot(Pythium inflatum matthews)in important inbred lines of maize[J].Journal of Plant Genetic Resources,2012,13(5):798-802.(in Chinese)
[17]贾曦,王璐,刘振林,等.玉米杂交种和自交系抗茎腐病鉴定[J].山东农业科学,2017,49(1):114-116.Jia X,Wang L,Liu Z L,et al.Resistance identification of maize hybrids and inbred lines to stalk rot[J].Shandong Agricultural Sciences,2017,49(1):114-116.(in Chinese)
[18]杨双,韩晓日.玉米早衰相关性状的遗传分析[J].玉米科学,2015,23(3):1-7,14.Yang S,Han X R.Genetic analysis of premature senescence related traits in maize[J].Journal of Maize Sciences,2015,23(3):1-7,14.(in Chinese)
[19]郑洪建,孔令杰,许瑞瑞,等.玉米叶片保绿性数量遗传分析[J].玉米科学,2013,21(6):17-20.Zheng H J,Kong L J,Xu R R,et al.Genetic analysis of stay-green traits in maize[J].Journal of Maize Sciences,2013,21(6):17-20.(in Chinese)