当归及其药对当归——白芍中阿魏酸的HPLC-DAD定量分析
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摘要
分析当归及其药对当归—白芍中阿魏酸的含量。采用HPLC-DAD法测定阿魏酸的含量,色谱柱为C18ODS(250 mm×4.6 mm, 5μm);柱温为(35±1)℃;流动相为乙腈—0.085%磷酸溶液;流速为0.9 mL/min;检测波长为322nm;等度洗脱。实验结果表明,当归及其药对当归—白芍检测液中阿魏酸的平均含量分别为25.015、20.975μg/mL,药对中阿魏酸含量下降了16.15%。该方法简便快速,精密度高,重复性好,其结果准确可靠,适用于当归及其药对资源的质量控制。
The content of ferulic acid in Angelica sinensis and its Herbal pair Angelica sinensis-Paeonia lactiflora are analyzed. And the content of ferulic acid is measured by HPLC-DAD method. The column is C18 ODS(250 mm× 4.6 mm, 5 μm)and the column temperature is set at(35 ± 1) ℃; The mobile phase is composed of acetonitrile-0.085% phosphoric acid and the flow rate is set of 0.9 mL/min; the detection wavelength is 322 nm with isocratic elution. The experiment result shows that the average content of ferulic acid about the test solution in Angelica sinensis and its Herbal pair Angelica sinensis-Paeonia lactiflora are 25.015 μg/mL and 20.975 μg/m L respectively. The reduction of content of ferulic acid in Herbal pair is 16.15%. The method is simple and rapid, more precision, reproducibility and reliable results as a recommendable option for qulity control in these resource.
The content of ferulic acid in Angelica sinensis and its Herbal pair Angelica sinensis-Paeonia lactiflora are analyzed. And the content of ferulic acid is measured by HPLC-DAD method. The column is C18 ODS(250 mm× 4.6 mm, 5 μm)and the column temperature is set at(35 ± 1) ℃; The mobile phase is composed of acetonitrile-0.085% phosphoric acid and the flow rate is set of 0.9 mL/min; the detection wavelength is 322 nm with isocratic elution. The experiment result shows that the average content of ferulic acid about the test solution in Angelica sinensis and its Herbal pair Angelica sinensis-Paeonia lactiflora are 25.015 μg/mL and 20.975 μg/m L respectively. The reduction of content of ferulic acid in Herbal pair is 16.15%. The method is simple and rapid, more precision, reproducibility and reliable results as a recommendable option for qulity control in these resource.
引文
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[2]王锦之.方剂学讲稿[M].北京:人民卫生出版社,2014:285-287.
[3]王锦鸿,陈仁寿.临床实用中药辞典[M].北京:金盾出版社,2003:230,292.
[4]王付.经方药对[M].北京:学苑出版社,2006:55-56.
[5]李贵海,涂晓龙.常用中药药对分析应用[M].北京:人民卫生出版社,2009:542-543.
[6]金汝城,李贵文,马素丽,等.当归中阿魏酸提取工艺的优化研究[J].中成药,2008,30(4):516-519.
[7]邢婧,张琳,汪轩,等.HPLC-DAD法同时测定右归丸中6种成分[J].中成药,2016,38(8):1 736-1 740.
[8]李军山,聂丽建.HPLC-DAD测定仙鹤草配方颗粒中3种成分[J].河北工业科技,2018,35(4):288-293.
[9]朱敏,段金廒,刘培,等.四物汤组方配伍主要成分的HPLC/DAD定量分析[J].中华中医药杂志,2013,28(8):2 385-2 389.
[10]唐亮,谭淑珍,华坤鹏,等.RH-HPLC法测定青蒿素中脱氢青蒿素及9-表-青蒿素的含量[J].湖南文理学院学报(自然科学版),2016,28(1):24-28.
[11]陈贞干,唐小梅,陈远道,等.木芙蓉提取物中芸香苷含量的高效液相色谱法测定[J].湖南文理学院学报(自然科学版),2010,22(3):53-56.