干旱胁迫对光叶珙桐幼苗抗氧化系统的影响
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摘要
通过研究干旱胁迫对光叶珙桐幼苗抗氧化酶系统相关物质的影响,以探究光叶珙桐幼苗对干旱胁迫的适应能力。设置对照组、5%、10%、15%、20%PEG-6000梯度浓度对3年生光叶珙桐幼苗进行干旱胁迫处理,提取叶片中RWC、SOD、POD和CAT和MDA进行比较研究。结果表明:(1)随着光叶珙桐幼苗遭受干旱胁迫程度加剧与胁迫时间的延长,叶片中RWC急剧减少,SOD、POD与CAT酶活均先上升后下降,MDA含量先下降后上升;(2)SOD变化不显著,在光叶珙桐抗旱机制中发挥的作用最小;而POD活性和CAT活性均变化明显,二者协同作用,在不同程度的胁迫下分别起到清除自由基的作用,以降低自由基对植株造成伤害;(3)POD活性在24h轻度胁迫处理下高出CK组409.14%,CAT在重度胁迫处理下高出CK组191.49%,二者在严重胁迫(20%PEG)处理下又降至接近CK组水平,说明POD在轻度胁迫时POD发挥其保护作用,随着干旱胁迫程度的加剧,CAT发挥着保护膜脂的主要作用,在严重胁迫下二者活性均降低;(4)MDA含量先下降后上升,说明在干旱胁迫初期,在保护酶协同作用下减少了MDA的生成,在严重胁迫处理时含量积累升高到CK组含量的29.86%,表明随胁迫程度的加剧保护酶活性受到明显抑制。光叶珙桐对干旱胁迫较为敏感,水因子成为光叶珙桐生长的主要限制条件。
For understanding the adaptability and countermeasures of Davidia involucrata var.vilmoriniana(Dode)Wange seedlings to drought stress,the effects of drought stress on the related substances of antioxidant enzymes in its seedlings were studied.Drought stress treatment were made for 3-year-old Davidia involucrata var.vilmoriniana(Dode)Wange seedlings with different concentration of control group,and 5%,10%,15% and 20% PEG-6000 gradients,and a comparative study was conducted after extraction of CAT,CAT and MDA from its leaves.The results showed that:(1)With the severity of drought stress and the prolongation of stress time,RWC decreased rapidly,the enzyme activity of SOD,POD and CAT increased at the beginning,and then descended,MDA contents decreased at the beginning and then increased;(2) There was no significance change on SOD,and SOD played a minimal role in the drought resistance mechanism.POD activity and CAT activity changed significantly,and they worked synergistically as scavenging free radicals under different stress;(3) POD activity was 409.14% higher than CK under slight stress with 24 h,CAT activity was 191.49% higher than CK under severe stress.Both POD and CAT decreased and close to CK under very severe st tress(20%PEG).This indicated that with the increase of drought stress,CAT played a major role in protecting membrane lipids,and their activities decreased under very severe stress;(4) The content of MD decreased at the beginning and then increased,which indicated that in the early stage of drought stress,the production of MDA was reduced under the synergistic action of protective enzymes.The accumulation of protective enzymes under very severe stress increased to 29.86% of CK,which indicated that the activity of protective enzymes was significantly inhibited with the increase of drought stress.The study showed that Davidia involucrata var.vilmoriniana(Dode)Wange is sensitive to drought stress and water factor is the critical element for its growth.
For understanding the adaptability and countermeasures of Davidia involucrata var.vilmoriniana(Dode)Wange seedlings to drought stress,the effects of drought stress on the related substances of antioxidant enzymes in its seedlings were studied.Drought stress treatment were made for 3-year-old Davidia involucrata var.vilmoriniana(Dode)Wange seedlings with different concentration of control group,and 5%,10%,15% and 20% PEG-6000 gradients,and a comparative study was conducted after extraction of CAT,CAT and MDA from its leaves.The results showed that:(1)With the severity of drought stress and the prolongation of stress time,RWC decreased rapidly,the enzyme activity of SOD,POD and CAT increased at the beginning,and then descended,MDA contents decreased at the beginning and then increased;(2) There was no significance change on SOD,and SOD played a minimal role in the drought resistance mechanism.POD activity and CAT activity changed significantly,and they worked synergistically as scavenging free radicals under different stress;(3) POD activity was 409.14% higher than CK under slight stress with 24 h,CAT activity was 191.49% higher than CK under severe stress.Both POD and CAT decreased and close to CK under very severe st tress(20%PEG).This indicated that with the increase of drought stress,CAT played a major role in protecting membrane lipids,and their activities decreased under very severe stress;(4) The content of MD decreased at the beginning and then increased,which indicated that in the early stage of drought stress,the production of MDA was reduced under the synergistic action of protective enzymes.The accumulation of protective enzymes under very severe stress increased to 29.86% of CK,which indicated that the activity of protective enzymes was significantly inhibited with the increase of drought stress.The study showed that Davidia involucrata var.vilmoriniana(Dode)Wange is sensitive to drought stress and water factor is the critical element for its growth.
引文
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[18]韩蕊莲,梁宗锁.干旱胁迫下沙棘叶片细胞膜透性与渗透调节物质研究[J].西北植物学报,2003,23(1):23-27.
[19]郝敬虹,易旸,尚庆茂,等.干旱胁迫下外源水杨酸对黄瓜幼苗膜脂过氧化和光合特性的影响[J].应用生态学报,2012,23(3):717-723.
[20]桑子阳,马履一,陈发菊.干旱胁迫对红花玉兰幼苗生长和生理特性的影响[J].西北植物学报,2011,31(1):109-115.
[21]范苏鲁,苑兆和,冯立娟,等.干旱胁迫对大丽花生理生化指标的影响[J].应用生态学报,2011,22(3):651-657.
[22]阎秀峰,祖元刚.干旱胁迫对红松幼苗保护酶活性及脂质过氧化作用的影响[J].生态学报,1999,19(6):850-854.
[23]孙景宽,夏江宝,田家怡,等.干旱胁迫对沙枣幼苗根茎叶保护酶系统的影响[J].江西农业大学学报,2009,31(5):879-884.
[24]孙国荣,彭永臻,阎秀峰,等.干旱胁迫对白桦实生苗保护酶活性及脂质过氧化作用的影响[J].林业科学,2003,39(1):165-167.
[25]贾文杰,王祥宁,马璐琳,等.干旱胁迫对东方百合根叶生理功能及花粉育性的影响[J].西北植物学报,2017,37(11):2194-2203.
[26]李瑞姣,陈献志,岳春雷,等.干旱胁迫对日本荚蒾幼苗光合生理特性的影响[J].生态学报,2018,38(6):2041-2047.
[27]谢志玉,张文辉,刘新成.干旱胁迫对文冠果幼苗生长和生理生化特征的影响[J].西北植物学报,2010,30(5):948-954.
[28]王春春,谢利娟,韩蕾,等.干旱处理对不同品种簕杜鹃光合特性的影响[J].西南林业大学学报,2018,38(4):70-75.
[2]常乐,夏宜平.中国珙桐在世界上的分布与应用现状.[EB/OL].http://www.doc88.com/p-156262165981.html
[3]王磊,代勋,黄良江,等.三江口自然保护区的珙桐种群分布状态的研究[J].西部林业科学,2009,38(3):72-75.
[4]王磊,杨顺强,代勋,等.云南大关三江口自然保护区光叶珙桐资源保护现状及对策[J].绿色科技,2010(8):10-13.
[5]王宁宁,胡增辉,沈应柏.珙桐苗木叶片光合特性对土壤干旱胁迫的响应[J].西北植物学报,2011,31(1):101-108.
[6]王宁宁.珙桐苗木光合特性对干旱、光照强度和二氧化碳浓度的响应[D].北京:北京林业大学,2010.
[7]吴庆贵,杨敬天,邹利娟,等.珙桐幼苗生理生态特性对土壤干旱胁迫的响应[J].江苏农业科学,2014,42(2):119-122.
[8]姜瑞芳,刘艳红.土壤基质和水分对珙桐幼苗生长的影响[J].西北林学院学报,2016,31(4):134-139,164.
[9]张云贵,谢永红.PEG在模拟植物干旱胁迫和组织培养中的应用(综述)[J].亚热带植物通讯,1994(2):61-64.
[10]戴必胜,杨敏,陈秀虎.霍格兰溶液培养对水仙生长发育的影响[J].武汉植物学研究,2006(5):485-488.
[11]高俊凤.植物生理学实验技术[M].西安:世界图书出版公司西安公司,2000:9-21.
[12]赵世杰,刘华山,董新纯.植物生理实验指导[M].北京:中国农业出版社,1997:152-154,161-163.
[13]张志良,瞿伟菁.植物生理学实验指导(4版)[M].北京:高等教育出版社,2009:100-101,125-126.
[14]董晶晶,齐淑艳,郭婷婷.PEG模拟干旱胁迫对入侵植物牛膝菊(Galinsoga parviflora)抗氧化物酶系统的影响[J].沈阳大学学报(自然科学版),2014,26(3):184-189.
[15]颜淑云,周志宇,邹丽娜,等.干旱胁迫对紫穗槐幼苗生理生化特性的影响[J].干旱区研究,2011,28(1):139-145.
[16]贾慧,其力木格,李特日根,等.外源SNP对干旱胁迫下不同马铃薯品种叶片抗氧化酶活性的影响[J].西北植物学报,2016,36(3):551-557.
[17]齐淑艳,段继鹏,郭婷婷,等.入侵植物牛膝菊种子萌发对PEG模拟干旱胁迫的响应[J].生态学杂志,2014,33(5):1190-1194.
[18]韩蕊莲,梁宗锁.干旱胁迫下沙棘叶片细胞膜透性与渗透调节物质研究[J].西北植物学报,2003,23(1):23-27.
[19]郝敬虹,易旸,尚庆茂,等.干旱胁迫下外源水杨酸对黄瓜幼苗膜脂过氧化和光合特性的影响[J].应用生态学报,2012,23(3):717-723.
[20]桑子阳,马履一,陈发菊.干旱胁迫对红花玉兰幼苗生长和生理特性的影响[J].西北植物学报,2011,31(1):109-115.
[21]范苏鲁,苑兆和,冯立娟,等.干旱胁迫对大丽花生理生化指标的影响[J].应用生态学报,2011,22(3):651-657.
[22]阎秀峰,祖元刚.干旱胁迫对红松幼苗保护酶活性及脂质过氧化作用的影响[J].生态学报,1999,19(6):850-854.
[23]孙景宽,夏江宝,田家怡,等.干旱胁迫对沙枣幼苗根茎叶保护酶系统的影响[J].江西农业大学学报,2009,31(5):879-884.
[24]孙国荣,彭永臻,阎秀峰,等.干旱胁迫对白桦实生苗保护酶活性及脂质过氧化作用的影响[J].林业科学,2003,39(1):165-167.
[25]贾文杰,王祥宁,马璐琳,等.干旱胁迫对东方百合根叶生理功能及花粉育性的影响[J].西北植物学报,2017,37(11):2194-2203.
[26]李瑞姣,陈献志,岳春雷,等.干旱胁迫对日本荚蒾幼苗光合生理特性的影响[J].生态学报,2018,38(6):2041-2047.
[27]谢志玉,张文辉,刘新成.干旱胁迫对文冠果幼苗生长和生理生化特征的影响[J].西北植物学报,2010,30(5):948-954.
[28]王春春,谢利娟,韩蕾,等.干旱处理对不同品种簕杜鹃光合特性的影响[J].西南林业大学学报,2018,38(4):70-75.