大蒜分子生物学研究进展
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摘要
综述了大蒜(Allium sativum L.)分子生物学的研究进展,主要包括5个方面:(1)大蒜遗传多样性分析、遗传图谱构建和资源评价中基因组7种分子标记(RAPD、AFLP、ISSR、SSR、SRAP、InDel和SNP)的开发和利用;(2)大蒜基因组学、表观遗传分析、转录组学和蛋白组学的研究;(3)农杆菌介导法和基因枪法的大蒜遗传转化体系构建;(4)大蒜蒜氨酸酶基因、蒜薹和鳞茎发育基因(gaLFY、AsPI、AsFT1、AsFT2和AsFT4)及抗逆相关基因(AsPCSl、AsMT2a、ASAL和AsNF-YC8)的克隆和表达分析;(5)大蒜分子生物学试验技术(RNA提取、RT-qPCR的内参基因筛选和BAC文库构建)优化。最后指出了当前研究的不足,展望了大蒜分子生物学研究的方向。
This paper reviews the following five aspects in garlic(Allium sativum L.)molecular biology:Firstly,the development and utilization of seven types of garlic genome molecular markers including RAPD,AFLP,ISSR,SSR,SRAP,In Del and SNP in genetic diversity analysis,genetic map construction and germplasm resources evaluation;Secondly,the research of garlic genomics,epigenetic analysis, transcriptomics and proteomics;Thirdly,construction of genetic transformation system including Agrobacterium tumefaciens mediated method and gene gun method;Fourthly,cloning and expression analysis of alliinase gene,genes related to flowering and bulb development(gaLFY,AsPI,AsFT1,AsFT2 and AsFT4)and genes related to stress resistance(AsPCSl,AsMT2a,ASAL and AsNF-YC8);Fifthly,experimental technique optimization of molecular biology of garlic involving RNA extraction,reference gene selection for RT-qPCR and BAC library construction. Finally,the current research defects and prospects of garlic molecular biology are summarized.
This paper reviews the following five aspects in garlic(Allium sativum L.)molecular biology:Firstly,the development and utilization of seven types of garlic genome molecular markers including RAPD,AFLP,ISSR,SSR,SRAP,In Del and SNP in genetic diversity analysis,genetic map construction and germplasm resources evaluation;Secondly,the research of garlic genomics,epigenetic analysis, transcriptomics and proteomics;Thirdly,construction of genetic transformation system including Agrobacterium tumefaciens mediated method and gene gun method;Fourthly,cloning and expression analysis of alliinase gene,genes related to flowering and bulb development(gaLFY,AsPI,AsFT1,AsFT2 and AsFT4)and genes related to stress resistance(AsPCSl,AsMT2a,ASAL and AsNF-YC8);Fifthly,experimental technique optimization of molecular biology of garlic involving RNA extraction,reference gene selection for RT-qPCR and BAC library construction. Finally,the current research defects and prospects of garlic molecular biology are summarized.
引文
Al-Zahim M A,Ford-Lloyd B V,Newbury H J.1999.Detection of somaclonal variation in garlic(Allium sativum L.)using RAPD and cytological analysis.Plant Cell Reports,18(6):473-477.
Arumuganathan K,Earle E D.1991.Nuclear DNA content of some important plant species.Plant Molecular Biology Reporter,9(3):208-218.
Bao Jin-ku.2011.Plant lectins:multifunctions and applications.Chinese Bulletin of Life Sciences,23(6):533-540.(in Chinese)鲍锦库.2011.植物凝集素的功能.生命科学,23(6):533-540.
Barandiaran X,Pietro A D,Martín J.1998.Biolistic transfer and expression of a uidA reporter gene in different tissues of Allium sativum L.Plant Cell Reports,17(9):737-741.
Bradley K F,Rieger M A,Collins G G.1996.Classification of Australian garlic cultivars by DNA fingerprinting.Australian Journal of Experimental Agriculture,36(5):613-618.
Cavagnaro P F,Galmarini C R.2007.Vegetables:Garlic.Germany:Springer Berlin Heidelberg Press,349-364.
Chan S W,Henderson I R,Jacobsen S E.2005.Gardening the genome:DNA methylation in Arabidopsis thaliana.Nature Reviews Genetics,6(5):351-360.
Chand S K,Nanda S,Mishra R,Joshi R K.2017.Multiple garlic(Allium sativum L.)microRNAs regulate the immunity against the basal rot fungus Fusarium oxysporum f.sp.Cepae.Plant Science,257:9-21.
Chen De-xi,He Zhong-quan,Xiang Yun-jia,Hu Rong-ping.2016.Cloning of ASAL gene and construction of its plant expression vector.Southwest China Journal of Agricultural Sciences,29(10):2445-2449.(in Chinese)陈德西,何忠全,向运佳,胡荣平.2016.大蒜叶凝集素基因ASAL的克隆与表达载体的构建.西南农业学报,29(10):2445-2449.
Chen S,Chen W,Shen X,Yang Y,Qi F,Liu Y,Meng H.2014.Analysis of the genetic diversity of garlic(Allium sativum L.)by simple sequence repeat and inter simple sequence repeat analysis and agro-morphological traits.Biochemical Systematics and Ecology,55(8):260-267.
Chen S,Zhou J,Chen Q,Chang Y,Du J,Meng H.2013.Analysis of the genetic diversity of garlic(Allium sativum L.)germplasm by SRAP.Biochemical Systematics and Ecology,50(4):139-146.
Chen Xin,Zhou Han-tao,Yang Zhi-wei,Pan Wen,Lin Peng.2005.Assessment of genetic diversity of garlic(Allium sativum L.)by the technique of molecular marker.Journal of Xiameng University(Natural Science Edition),44(s1):144-149.(in Chinese)陈昕,周涵韬,杨志伟,潘文,林鹏.2005.大蒜种质资源遗传多样性的分子标记研究.厦门大学学报(自然科学版),44(s1):144-149.
Cheng Zhi-hui.2010.The theory of vegetable cultivation.Beijing:Science Press,187-193.(in Chinese)程智慧.2010.蔬菜栽培学各论.北京:科学出版社:187-193.
Cunha C P D,Resende F V,Zucchi M I,Pinheiro J B.2014.SSR-based genetic diversity and structure of garlic accessions from Brazil.Genetica,142(5):419-431.
Cunha C P,Hoogerheide E S,Zucchi M I,Monteiro M,Pinheiro J B.2012.New microsatellite markers for garlic,Allium sativum(Alliaceae).American Journal of Botany,99(1):e17-e19.
Dae-Won K,Tae-Sung J,Seong-Hyeuk N,Hyuk-Ryul K,Aeri K,Sung-Hwa C,Sang-Haeng C,Dong-Wook K,Ryong Nam K,Hong-Seog P.2009.Garlic ESTdb:an online database and mining tool for garlic EST sequences.BMC Plant Biology,9:61.
Dufoo-Hurtado M D,Huerta-Ocampo J A,Barrera-Pacheco A,Rosa A P B D L,Mercado-Silva E M.2015.Low temperature conditioning of garlic(Allium sativum L.)“seed”cloves induces alterations in sprouts proteome.Frontiers in Plant Science,6:332.
Dufoo-Hurtado M D,Zavala-Gutiérrez K G,Cao C M,Cisneros-Zevallos L,Guevara-González R G,Torres-Pacheco I,Vázquez-Barrios M E,Rivera-Pastrana D M,Mercado-Silva E M.2013.Low-temperature conditioning of“seed”cloves enhances the expression of phenolic metabolism related genes and anthocyanin content in‘Coreano’garlic(Allium sativum L.)during plant development.Journal of Agricultural and Food Chemistry,61(44):10439-10446.
Eady C,Davis S,Catanach A,Kenel F,Hunger S.2005.Agrobacterium tumefaciens-mediated transformation of leek(Allium porrum)and garlic(Allium sativum L.).Plant Cell Reports,24(4):209-215.
Eady C,Davis S,Farrant J,Reader J,Kenel F.2003.Agrobacterium tumefaciens-mediated transformation and regeneration of herbicide resistant onion(Allium cepa)plants.Annals of Applied Biology,142(2):213-217.
Endo A,Imai Y,Nakamura M,Yanagisawa E,Taguchi T,Torii K,Okumura H,Ichinose K.2014.Distinct intraspecific variations of garlic(Allium sativum L.)revealed by the exon-intron sequences of the alliinase gene.Journal of Natural Medicines,68(2):442-447.
Etoh T,Simon P W.2002.Diversity,fertility and seed production of garlic.New York:CABI Press:101-118.
Evans G.1970.Base composition of nuclear DNA within the genus,Allium.Heredity,25(4):507-512.
FAO.2016.FAOSTAT.Food and Agricultural Organization of United Nations,Economic and Social Department,The Statistical Division:http://www.fao.org/faostat/en/#data/QC.
Figliuolo G,Stefano D D.2007.Is single bulb producing garlic Allium sativum,or Allium ampeloprasum?Scientia Horticulturae,114(4):243-249.
Gao Xing-ying,Li Mei-lan,Wang Ting-ting,Hou Lei-ping.2013.Research progress on genetic transformation of Allium vegetables.Biotechnology Bulletin,(5):1-6.(in Chinese)高行英,李梅兰,王婷婷,侯雷平.2013.葱蒜类蔬菜遗传转化研究进展.生物技术通报,(5):1-6.
García L S,Asprelli P,Burba J L.2012.Genetic analysis of a garlic(Allium sativum L.)germplasm collection from Argentina.Scientia Horticulturae,138(5):183-189.
Garcia L S,Martinez L,Burba J L.2003.Genetic diversity among selected Argentinean garlic clones(Allium sativum L.)using AFLP(amplified fragment length polymorphism).Euphytica,132(1):115-119.
Gimenez M D,Ya?ez-Santos A M,Paz R C,Quiroga M P,Marfil C F,Conci V C,Garcia-Lampasona S C.2016.Assessment of genetic and epigenetic changes in virus-free garlic(Allium sativum L.)plants obtained by meristem culture followed,by in vitro propagation.Plant Cell Reports,35(1):129-141.
He Yan-xia,Wang Zi-cheng,Cao Hong-ping,Zhang Cheng-wan.2007.Initial study of DNA methylation difference of garlic(Allium sativum L.)growing in light and darkness.Plant Physiology Communications,43(1):85-88.(in Chinese)何艳霞,王子成,曹红平,张成婉.2007.光暗条件下大蒜DNA甲基化差异的初步研究.植物生理学通讯,43(1):85-88.
Huggett J,Dheda K,Bustin S,Zumla A.2005.Real-time RT-PCR normalisation;strategies and considerations.Genes and Immunity,6(4):279-284.
Ipek M,Ipek A,Almquist S G,Simon P W.2005.Demonstration of linkage and development of the first low-density genetic map of garlic,based on AFLP markers.Theoretical and Applied Genetics,110(2):228-236.
Ipek M,Ipek A,Simon P W.2003.Comparison of AFLPs,RAPD markers and isozymes for diversity assessment of garlic and detection of putative duplicates in germplasm collections.Journal of the American Society for Horticultural Science,128(2):246-252.
Ipek M,Simon P W.2001.Genetic diversity in garlic as assessed by amplified fragment length polymorphisms and isozymes.Hortscience,36(3):454.
Jabbes N,Geoffriau E,Clerc V L,Dridi B.2011.Inter simple sequence repeat fingerprints for assess genetic diversity of Tunisian garlic populations.Journal of Agricultural Science,3(4):77-85.
Jiang Ying-nan,Feng Bao-min,Zhang Hai-yan,Ma Mi.2005.Improving heave metal tolerance of yeast by transferring a phytochelatin synthase gene from garlic.Acta Phytoecologica Sinica,29(4):659-664.(in Chinese)姜瑛楠,冯保民,张海燕,麻密.2005.大蒜植物络合素合酶基因转化对酵母重金属抗性的提高.植物生态学报,29(4):659-664.
Jin Zhi-yong,Wang Shuo-shuo,Liu Na,Gong Biao,Liu Shi-qi,Shi Qing-hua.2014.Effect of different light supplement time and method on bulbing enlargement and nutritional quality of greenhouse-grown Garlic.Acta Horticulturae Sinica,41(12):2411-2418.(in Chinese)靳志勇,王硕硕,刘娜,巩彪,刘世琦,史庆华.2014.不同补光时间和方式对设施大蒜鳞茎膨大和品质的影响.园艺学报,41(12):2411-2418.
Jones M G,Hughes J,Tregova A,Milne J,Tomsett A B,Collin H A.2004.Biosynthesis of the flavour precursors of onion and garlic.Journal of Experimental Botany,55(404):1903-1918.
Kamenetsky R,Faigenboim A,Mayer E S,Michael T B,Chen G,Kimhi S,Esquira I,Shalom S R,Eshel D,Rabinowitch H D,Sherman A.2015.Integrated transcriptome catalogue and organ-specific profiling of gene expression in fertile garlic(Allium sativum L.).BMC Genomics,16:12.
Kamenetsky R,Shafir I L,Khassanov F,Kik C.2005.Diversity in fertility potential and organo-sulphur compounds among garlics from central Asia.Biodiversity and Conservation,14(2):281-295.
Kamenetsky R,Shafir I L,Zemah H,Barzilay A,Rabinowitch H D.2004.Environmental control of garlic growth and florogenesis.Journal of the American Society for Horticultural Science,129(2):144-151.
Kamenetsky R,Rabinowitch H D.2010.The genus Allium:a developmental and horticultural analysis.New York:John Wiley&Sons,Inc:329-378.
Kenel F,Eady C,Brinch S.2010.Efficient Agrobacterium tumefaciens-mediated transformation and regeneration of garlic(Allium sativum L.)immature leaf tissue.Plant Cell Reports,29(3):223-230.
Klein T M,Fitzpatrick-Mcelligott S.1993.Particle bombardment:a universal approach for gene transfer to cells and tissues.Current Opinion in Biotechnology,4(5):583-590.
Kondo T,Hasegawa H,Suzuki M.2000.Transformation and regeneration of garlic(Allium sativum L.)by Agrobacterium-mediated gene transfer.Plant Cell Reports,19(10):989-993.
Kuhl J C,Cheung F,Yuan Q,Martin W,Zewdie Y,Mccallum J,Catanach A,Rutherford P,Sink K C,Jenderek M,Prince J P,Town C D,Havey M J.2004.A unique set of 11008 onion expressed sequence tags reveals expressed sequence and genomic differences between the monocot orders Asparagales and Poales.Plant Cell,16(1):114-125.
Lai Qing-qin,Yin Cheng-hui,Hu You-jia,Yu Mei-jing,Liu Quan-hai.2010.Cloning of alliinase gene from garlic and its expression in Escherichia coli.Chinese Journal of Pharmaceuticals,41(7):496-500.(in Chinese)来庆勤,殷承慧,胡又佳,于美静,刘全海.2010.大蒜蒜氨酸酶基因的克隆及在大肠杆菌中的表达.中国医药工业杂志,41(7):496-500.
Lee H R,Eom E M,Lim Y P,Bang J W,Lee D H.2003.Construction of a garlic BAC library and chromosomal assignment of BAC clones using the FISH technique.Genome,46(3):514-520.
Lee R,Baldwin S,Kenel F,Mccallum J,Macknight R.2013.FLOWERING LOCUS T genes control onion bulb formation and flowering.Nature Communications,4(1):2884.
Liu M,Wu Z,Jiang F.2015a.Selection and validation of garlic reference genes for quantitative real-time PCR normalization.Plant Cell Tissue and Organ Culture,122(2):435-444.
Liu T,Zeng L,Zhu S,Chen X,Tang Q,Mei S,Tang S.2015b.Large-scale development of expressed sequence tag-derived simple sequence repeat markers by deep transcriptome sequencing in garlic(Allium sativum L.).Molecular Breeding,35(11):1-9.
Luo M,Wang Y H,Frisch D,Joobeur T,Wing R A,Dean R A.2001.Melon bacterial artificial chromosome(BAC)library construction using improved methods and identification of clones linked to the locus conferring resistance to melon Fusarium wilt(Fom-2).Genome,44(2):154-162.
Ma K H,Kwag J G,Zhao W,Dixit A,Lee G A,Kim H H,Chung I M,Kim N S,Lee J S,Ji J J,Kim T S,Park Y J.2009.Isolation and characteristics of eight novel polymorphic microsatellite loci from the genome of garlic(Allium sativum L.).Scientia Horticulturae,122(3):355-361.
Maa?H I,Klaas M.1995.Infra-specific differentiation of garlic(Allium sativum L.)by isozyme and RAPD markers.Theoretical and Applied Genetics,91(1):89-97.
Morales R G,Resende J T,Resende F V,Delatorre C A,Figueiredo A S T,Da-Silva P R.2013.Genetic divergence among Brazilian garlic cultivars based on morphological characters and AFLP markers.Genetics and Molecular Research,12(1):270-281.
Neta R,Davidschwartz R,Peretz Y,Sela I,Rabinowitch H D,Flaishman M,Kamenetsky R.2011.Flower development in garlic:the ups and downs of gaLFY expression.Planta,233(5):1063-1072.
Ohri D,Pistrick K.2010.Phenology and genome size variation in Allium L.-a tight correlation?Plant Biology,3(6):654-660.
OvesnáJ,Ku?era L,Horní?kováJ,SvobodováL,StavělíkováH,Velí?ek J,Milella L.2011.Diversity of S-alk(en)yl cysteine sulphoxide content within a collection of garlic(Allium sativum L.)and its association with the morphological and genetic background assessed by AFLP.Scientia Horticulturae,129(4):541-547.
Panda D,Dehury B,Sahu J,Barooah M,Sen P,Modi M K.2014.Computational identification and characterization of conserved miRNAs and their target genes in garlic(Allium sativum L.)expressed sequence tags.Gene,537(2):333-342.
Park M Y,Yi N R,Lee H Y,Kim S T,Kim M,Park J H,Kim J K,Lee J S,Cheong J J,Choi D Y.2002.Generation of chlorsulfuron-resistant transgenic garlic plants(Allium sativum L.)by particle bombardment.Molecular Breeding,9(3):171-181.
Pooler M R,Simon P W.1994.True seed production in garlic.Plant Reproduction,7(5):282-286.
Rabinkov A,Zhu X Z,Grafi G,Galili G,Mirelman D.1994.Alliin lyase(alliinase)from garlic(Allium sativum L.)-biochemical characterization and cDNA cloning.Applied Biochemistry and Biotechnology,48(3):149-171.
Ricroch A,Yockteng R,Brown S C,Nadot S.2005.Evolution of genome size across some cultivated Allium species.Genome,48(3):511-520.
Robert T F,Stephen G R,Robert B H,Stanton B G.1986.Genetic transformation in higher plants.Critical Reviews in Plant Sciences,4(1):1-46.
Rohkin S S,Gillett D,Zemach H,Kimhi S,Forer I,Zutahy Y,Teper-Bamnolker P,Kamenetsky R,Eshel D.2015.Storage temperature controls the timing of garlic bulb formation via shoot apical meristem termination.Planta,242(4):951-962.
Rotem N,Shemesh E,Peretz Y,Akad F,Edelbaum O,Rabinowitch H D,Sela I,Kamenetsky R.2007.Reproductive development and phenotypic differences in garlic are associated with expression and splicing of LEAFY homologue gaLFY.Journal of Experimental Botany,58(5):1133-1141.
Santiago-Valentín E,Francisco-Ortega J.2008.Plant evolution and biodiversity in the Caribbean islands-perspectives from molecular markers.Botanical Review,74(1):1-4.
Sato H,Mizoi J,Tanaka H,Maruyama K,Qin F,Osakabe Y,Morimoto K,Ohori T,Kusakabe K,Nagata M,Shinozaki K,Yamaguchi-Shinozaki K.2014.Arabidopsis DPB3-1,a DREB2A interactor,specifically enhances heat stress-induced gene expression by forming a heat stress-specific transcriptional complex with NF-Y subunits.Plant Cell,26(12):4954-4973.
Shaaf S,Sharma R,Kilian B,Walther A,Ozkan H,Karami E,Mohammadi B.2014.Genetic structure and eco-geographical adaptation of garlic landraces(Allium sativum L.)in Iran.Genetic Resources and Crop Evolution,61(8):1565-1580.
Shimon L J W,Rabinkov A,Shin I,Miron T,Mirelman D,Wilchek M.2007.Two structures of alliinase from Alliium sativum L.:apo form and ternary complex with aminoacrylate reaction intermediate covalently bound to the PLP cofactor.Journal of Molecular Biology,366(2):611-625.
Son J H,Park K C,Lee S I,Kim J H,Kim N S.2012.Species relationships among Allium species by ISSR analysis.Horticulture Environment and Biotechnology,53(3):256-262.
Sun X,Lian H,Liu X,Zhou S,Liu S.2016.The garlic NF-YC gene,AsNF-YC8,positively regulates non-ionic hyperosmotic stress tolerance in tobacco.Protoplasma,254(3):1-14.
Sun X,Zhou S,Meng F,Liu S.2012.De novo assembly and characterization of the garlic(Allium sativum L.)bud transcriptome by Illumina sequencing.Plant Cell Reports,31(10):1823-1828.
Sun Xiu-dong.2013.Transcriptome sequencing in garlic(Allium sativum L.)and functional analysis of the AsNF-YB3[Ph.D.Dissertation].Tai’an:Shandong Agricultural University.(in Chinese)孙秀东.2013.大蒜转录组测序及核因子YB3的功能研究[博士论文].泰安:山东农业大学.
Tang Qiao-ling.2013.Cloning and expression of a novel alliinase gene from garlic roots and selencing of alliinase genes by RNAi[Ph.D.Dissertation].Beijing:Chinese Academy of Agricultural Sciences.(in Chinese)唐巧玲.2013.大蒜根蒜氨酸酶基因的克隆和表达及RNAi沉默研究[博士论文].北京:中国农业科学院.
Tang Qiao-ling,Wang Xu-jing,Wang Zhi-xing.2014.Establishment of particle bombardment-mediated transformation systemin garlic.Journal of Plant Genetic Resources,15(3):576-581.(in Chinese)唐巧玲,王旭静,王志兴.2014.基因枪介导的大蒜遗传转化体系的建立.植物遗传资源学报,15(3):576-581.
Tang Qiao-ling,Wang Zhi-xing,Wang Xu-jing.2015.An alliinase gene and its application[Patent].Beijing:CN104928273A.(in Chinese)唐巧玲,王志兴,王旭静.2015.一种大蒜蒜氨酸酶及其编码基因与应用[专利].北京:CN104928273A.
Trick H N,Finer J J.1997.SAAT:sonication-assisted agrobacterium-mediated transformation.Transgenic Research,6(5):329-336.
van Damme E J,Smeets K,Torrekens S,Van Leuven F,Peumans W J.1992.Isolation and characterization of alliinase cDNA clones from garlic(Allium sativum L.)and related species.European Journal of Biochemistry,209(2):751-757.
Volk G M,Henk A D,Richards C M.2004.Genetic diversity among U.S.garlic clones as detected using AFLP methods.Journal of the American Society for Horticultural Science,129(4):559-569.
Wagner D,Wellmer F,Dilks K,William D,Smith M R,Kumar P P,Riechmann J L,Greenland A J,Meyerowitz E M.2004.Floral induction in tissue culture:a system for the analysis of LEAFY-dependent gene regulation.Plant Journal,39(2):273-282.
Wang H,Li X,Liu X,Oiu Y,Song J,Zhang X,Havey M.2016.Genetic diversity of garlic(Allium sativum L.)germplasm from China by fluorescent-based AFLP,SSR and InDel markers.Plant Breeding,135(6):743-750.
Wang Hai-ping.2011.Evaluation on genetic diversity of garlic germplasm in China and association analysis between allicin content and alliinase genes[Ph.D.Dissertation].Beijing:Chinese Academy of Agricultural Sciences.(in Chinese)王海平.2011.中国大蒜遗传多样性评价及大蒜辣素含量与蒜氨酸酶基因的关联分析[博士论文].北京:中国农业科学院.
Wang M,Wu C,Cheng Z,Meng H.2015.Growth and physiological changes in continuously cropped eggplant(Solanum melongena L.)upon relay intercropping with garlic(Allium sativum L.).Frontiers in Plant Science,6:262.
Weik R,Francky A,Striedner G,Raspor P,Bayer K,Mattanovich D.1998.Recombinant expression of alliin lyase from garlic(Allium sativum)in bacteria and yeasts.Planta Medica,64(4):387-388.
Weiner L,Shin I,Shimon L J,Miron T,Wilchek M,Mirelman D,Frolow F,Rabinkov A.2010.Thiol-disulfide organization in alliin lyase(alliinase)from garlic(Allium sativum L.).Protein Science,18(1):196-205.
Wu C,Wang M,Cheng Z,Meng H.2016a.Response of garlic(Allium sativum L.)bolting and bulbing to temperature and photoperiod treatments.Biology Open,5(4):507-518.
Wu C,Wang M,Dong Y,Cheng Z,Meng H.2016b.Effect of plant age and vernalization on bolting,plant growth and enzyme activity of garlic(Allium sativum L.).Scientia Horticulturae,201:295-305.
Wu Xiao-li,Xu Jian,Zhang Ting,Jia Ping,Zhou Yun-kai.2010.Cloning and sequence analysis of alliinase gene from garlic and construction of Pichia pastoris expression plasmid.Journal of Jilin Agricultural University,32(3):258-263.(in Chinese)吴晓莉,许健,张婷,贾平,周云凯.2010.大蒜蒜氨酸酶基因克隆与序列分析及毕赤酵母表达质粒的构建.吉林农业大学学报,32(3):258-263.
Wu Xiao-li,Zhang Ting,Bian Jin-duo,Xu Jian.2012.Cloning of alliinase gene from garlic bulb and its expression in Pichia pastoris.Chinese Traditional and Herbal Drugs,43(1):143-147.(in Chinese)吴晓莉,张婷,边金铎,许健.2012.大蒜鳞茎蒜氨酸酶基因克隆及其在毕赤酵母中的表达.中草药,43(1):143-147.
Xu Ling,Qiu Xue-mei,Liu Xin-ping,Liu Ren-rong.2012.Research progress of alliance on gene level.Li-Shi-Zhen Medicine Materia Medica Research,23(4):998-1000.(in Chinese)徐玲,裘雪梅,刘新平,刘仁荣.2012.蒜氨酸酶基因水平研究进展.时珍国医国药,23(4):998-1000.
Xu Pei-wen,Yang Chong-liang,Cui De-cai,Qu Shi-song,Liu Bing-jiang,Zhang Jun-ting,Zhang Chuan-kun.2005.A preliminary study on techniques for sexual reproduction of garlic(Allium sativum L.).Acta Horticulturae Sinica,32(3):503-506.(in Chinese)徐培文,杨崇良,崔德才,曲士松,刘冰江,张君亭,张传坤.2005.大蒜有性生殖技术研究初报.园艺学报,32(3):503-506.
Xu Rong,Ren Hong-jie,Wang Hai-zhu,Lou Hu,Xu Qi-jiang.2017.Cloning and expression analysis of MADS-box gene AsPI in garlic.Acta Horticulturae Sinica,44(1):69-79.(in Chinese)徐熔,任洪杰,王海竹,娄虎,徐启江.2017.大蒜MADS-box基因AsPI的克隆及表达分析.园艺学报,44(1):69-79.
Zewdie Y,Havey M J,Prince J P,Jenderek M M.2005.First genetic linkages among expressed regions of the garlic genome.Journal of the American Society for Horticultural Science,130(4):569-574.
Zhang Ai-min,Zhang Su-qin,Sheng Zhong-fei,Liu Li-qing.2009.Extraction and quality analysis of total RNA in different tissues of garlic.Northern Horticulture,(11):108-109.(in Chinese)张爱民,张素勤,盛中飞,刘利青.2009.大蒜不同组织总RNA提取与质量分析.北方园艺,(11):108-109.
Zhang Fen.2008.Evaluation of garlic germplasms based on morphological characters,induction and regeneration of calluses and culture of protoplasts of garlic[Ph.D.Dissertation].Hangzhou:Zhejiang University.(in Chinese)张芬.2008.大蒜种质资源的形态学评价、愈伤诱导再生和原生质体培养[博士论文].杭州:浙江大学.
Zhang Hai-yan.2005.Cloning and functional characterization of heavy metal-responsive genes from garlic[Ph.D.Dissertation].Beijing:Chinese Academy of Science.(in Chinese)张海燕.2005.大蒜重金属抗性基因的克隆及其功能分析[博士论文].北京:中国科学院.
Zheng S J,Henken B,Ahn Y K,Krens F A,Kik C.2004.The development of a reproducible agrobacterium tumefaciens transformation system for garlic(Allium sativum L.)and the production of transgenic garlic resistant to beet armyworm(Spodoptera exigua Hubner).Molecular Breeding,14(3):293-307.
Zhu S,Tang S,Tan Z,Yu Y,Dai Q,Liu T.2017.Comparative transcriptomics provide insight into the morphogenesis and evolution of fistular leaves in Allium.BMC Genomics,18(1):60.
Arumuganathan K,Earle E D.1991.Nuclear DNA content of some important plant species.Plant Molecular Biology Reporter,9(3):208-218.
Bao Jin-ku.2011.Plant lectins:multifunctions and applications.Chinese Bulletin of Life Sciences,23(6):533-540.(in Chinese)鲍锦库.2011.植物凝集素的功能.生命科学,23(6):533-540.
Barandiaran X,Pietro A D,Martín J.1998.Biolistic transfer and expression of a uidA reporter gene in different tissues of Allium sativum L.Plant Cell Reports,17(9):737-741.
Bradley K F,Rieger M A,Collins G G.1996.Classification of Australian garlic cultivars by DNA fingerprinting.Australian Journal of Experimental Agriculture,36(5):613-618.
Cavagnaro P F,Galmarini C R.2007.Vegetables:Garlic.Germany:Springer Berlin Heidelberg Press,349-364.
Chan S W,Henderson I R,Jacobsen S E.2005.Gardening the genome:DNA methylation in Arabidopsis thaliana.Nature Reviews Genetics,6(5):351-360.
Chand S K,Nanda S,Mishra R,Joshi R K.2017.Multiple garlic(Allium sativum L.)microRNAs regulate the immunity against the basal rot fungus Fusarium oxysporum f.sp.Cepae.Plant Science,257:9-21.
Chen De-xi,He Zhong-quan,Xiang Yun-jia,Hu Rong-ping.2016.Cloning of ASAL gene and construction of its plant expression vector.Southwest China Journal of Agricultural Sciences,29(10):2445-2449.(in Chinese)陈德西,何忠全,向运佳,胡荣平.2016.大蒜叶凝集素基因ASAL的克隆与表达载体的构建.西南农业学报,29(10):2445-2449.
Chen S,Chen W,Shen X,Yang Y,Qi F,Liu Y,Meng H.2014.Analysis of the genetic diversity of garlic(Allium sativum L.)by simple sequence repeat and inter simple sequence repeat analysis and agro-morphological traits.Biochemical Systematics and Ecology,55(8):260-267.
Chen S,Zhou J,Chen Q,Chang Y,Du J,Meng H.2013.Analysis of the genetic diversity of garlic(Allium sativum L.)germplasm by SRAP.Biochemical Systematics and Ecology,50(4):139-146.
Chen Xin,Zhou Han-tao,Yang Zhi-wei,Pan Wen,Lin Peng.2005.Assessment of genetic diversity of garlic(Allium sativum L.)by the technique of molecular marker.Journal of Xiameng University(Natural Science Edition),44(s1):144-149.(in Chinese)陈昕,周涵韬,杨志伟,潘文,林鹏.2005.大蒜种质资源遗传多样性的分子标记研究.厦门大学学报(自然科学版),44(s1):144-149.
Cheng Zhi-hui.2010.The theory of vegetable cultivation.Beijing:Science Press,187-193.(in Chinese)程智慧.2010.蔬菜栽培学各论.北京:科学出版社:187-193.
Cunha C P D,Resende F V,Zucchi M I,Pinheiro J B.2014.SSR-based genetic diversity and structure of garlic accessions from Brazil.Genetica,142(5):419-431.
Cunha C P,Hoogerheide E S,Zucchi M I,Monteiro M,Pinheiro J B.2012.New microsatellite markers for garlic,Allium sativum(Alliaceae).American Journal of Botany,99(1):e17-e19.
Dae-Won K,Tae-Sung J,Seong-Hyeuk N,Hyuk-Ryul K,Aeri K,Sung-Hwa C,Sang-Haeng C,Dong-Wook K,Ryong Nam K,Hong-Seog P.2009.Garlic ESTdb:an online database and mining tool for garlic EST sequences.BMC Plant Biology,9:61.
Dufoo-Hurtado M D,Huerta-Ocampo J A,Barrera-Pacheco A,Rosa A P B D L,Mercado-Silva E M.2015.Low temperature conditioning of garlic(Allium sativum L.)“seed”cloves induces alterations in sprouts proteome.Frontiers in Plant Science,6:332.
Dufoo-Hurtado M D,Zavala-Gutiérrez K G,Cao C M,Cisneros-Zevallos L,Guevara-González R G,Torres-Pacheco I,Vázquez-Barrios M E,Rivera-Pastrana D M,Mercado-Silva E M.2013.Low-temperature conditioning of“seed”cloves enhances the expression of phenolic metabolism related genes and anthocyanin content in‘Coreano’garlic(Allium sativum L.)during plant development.Journal of Agricultural and Food Chemistry,61(44):10439-10446.
Eady C,Davis S,Catanach A,Kenel F,Hunger S.2005.Agrobacterium tumefaciens-mediated transformation of leek(Allium porrum)and garlic(Allium sativum L.).Plant Cell Reports,24(4):209-215.
Eady C,Davis S,Farrant J,Reader J,Kenel F.2003.Agrobacterium tumefaciens-mediated transformation and regeneration of herbicide resistant onion(Allium cepa)plants.Annals of Applied Biology,142(2):213-217.
Endo A,Imai Y,Nakamura M,Yanagisawa E,Taguchi T,Torii K,Okumura H,Ichinose K.2014.Distinct intraspecific variations of garlic(Allium sativum L.)revealed by the exon-intron sequences of the alliinase gene.Journal of Natural Medicines,68(2):442-447.
Etoh T,Simon P W.2002.Diversity,fertility and seed production of garlic.New York:CABI Press:101-118.
Evans G.1970.Base composition of nuclear DNA within the genus,Allium.Heredity,25(4):507-512.
FAO.2016.FAOSTAT.Food and Agricultural Organization of United Nations,Economic and Social Department,The Statistical Division:http://www.fao.org/faostat/en/#data/QC.
Figliuolo G,Stefano D D.2007.Is single bulb producing garlic Allium sativum,or Allium ampeloprasum?Scientia Horticulturae,114(4):243-249.
Gao Xing-ying,Li Mei-lan,Wang Ting-ting,Hou Lei-ping.2013.Research progress on genetic transformation of Allium vegetables.Biotechnology Bulletin,(5):1-6.(in Chinese)高行英,李梅兰,王婷婷,侯雷平.2013.葱蒜类蔬菜遗传转化研究进展.生物技术通报,(5):1-6.
García L S,Asprelli P,Burba J L.2012.Genetic analysis of a garlic(Allium sativum L.)germplasm collection from Argentina.Scientia Horticulturae,138(5):183-189.
Garcia L S,Martinez L,Burba J L.2003.Genetic diversity among selected Argentinean garlic clones(Allium sativum L.)using AFLP(amplified fragment length polymorphism).Euphytica,132(1):115-119.
Gimenez M D,Ya?ez-Santos A M,Paz R C,Quiroga M P,Marfil C F,Conci V C,Garcia-Lampasona S C.2016.Assessment of genetic and epigenetic changes in virus-free garlic(Allium sativum L.)plants obtained by meristem culture followed,by in vitro propagation.Plant Cell Reports,35(1):129-141.
He Yan-xia,Wang Zi-cheng,Cao Hong-ping,Zhang Cheng-wan.2007.Initial study of DNA methylation difference of garlic(Allium sativum L.)growing in light and darkness.Plant Physiology Communications,43(1):85-88.(in Chinese)何艳霞,王子成,曹红平,张成婉.2007.光暗条件下大蒜DNA甲基化差异的初步研究.植物生理学通讯,43(1):85-88.
Huggett J,Dheda K,Bustin S,Zumla A.2005.Real-time RT-PCR normalisation;strategies and considerations.Genes and Immunity,6(4):279-284.
Ipek M,Ipek A,Almquist S G,Simon P W.2005.Demonstration of linkage and development of the first low-density genetic map of garlic,based on AFLP markers.Theoretical and Applied Genetics,110(2):228-236.
Ipek M,Ipek A,Simon P W.2003.Comparison of AFLPs,RAPD markers and isozymes for diversity assessment of garlic and detection of putative duplicates in germplasm collections.Journal of the American Society for Horticultural Science,128(2):246-252.
Ipek M,Simon P W.2001.Genetic diversity in garlic as assessed by amplified fragment length polymorphisms and isozymes.Hortscience,36(3):454.
Jabbes N,Geoffriau E,Clerc V L,Dridi B.2011.Inter simple sequence repeat fingerprints for assess genetic diversity of Tunisian garlic populations.Journal of Agricultural Science,3(4):77-85.
Jiang Ying-nan,Feng Bao-min,Zhang Hai-yan,Ma Mi.2005.Improving heave metal tolerance of yeast by transferring a phytochelatin synthase gene from garlic.Acta Phytoecologica Sinica,29(4):659-664.(in Chinese)姜瑛楠,冯保民,张海燕,麻密.2005.大蒜植物络合素合酶基因转化对酵母重金属抗性的提高.植物生态学报,29(4):659-664.
Jin Zhi-yong,Wang Shuo-shuo,Liu Na,Gong Biao,Liu Shi-qi,Shi Qing-hua.2014.Effect of different light supplement time and method on bulbing enlargement and nutritional quality of greenhouse-grown Garlic.Acta Horticulturae Sinica,41(12):2411-2418.(in Chinese)靳志勇,王硕硕,刘娜,巩彪,刘世琦,史庆华.2014.不同补光时间和方式对设施大蒜鳞茎膨大和品质的影响.园艺学报,41(12):2411-2418.
Jones M G,Hughes J,Tregova A,Milne J,Tomsett A B,Collin H A.2004.Biosynthesis of the flavour precursors of onion and garlic.Journal of Experimental Botany,55(404):1903-1918.
Kamenetsky R,Faigenboim A,Mayer E S,Michael T B,Chen G,Kimhi S,Esquira I,Shalom S R,Eshel D,Rabinowitch H D,Sherman A.2015.Integrated transcriptome catalogue and organ-specific profiling of gene expression in fertile garlic(Allium sativum L.).BMC Genomics,16:12.
Kamenetsky R,Shafir I L,Khassanov F,Kik C.2005.Diversity in fertility potential and organo-sulphur compounds among garlics from central Asia.Biodiversity and Conservation,14(2):281-295.
Kamenetsky R,Shafir I L,Zemah H,Barzilay A,Rabinowitch H D.2004.Environmental control of garlic growth and florogenesis.Journal of the American Society for Horticultural Science,129(2):144-151.
Kamenetsky R,Rabinowitch H D.2010.The genus Allium:a developmental and horticultural analysis.New York:John Wiley&Sons,Inc:329-378.
Kenel F,Eady C,Brinch S.2010.Efficient Agrobacterium tumefaciens-mediated transformation and regeneration of garlic(Allium sativum L.)immature leaf tissue.Plant Cell Reports,29(3):223-230.
Klein T M,Fitzpatrick-Mcelligott S.1993.Particle bombardment:a universal approach for gene transfer to cells and tissues.Current Opinion in Biotechnology,4(5):583-590.
Kondo T,Hasegawa H,Suzuki M.2000.Transformation and regeneration of garlic(Allium sativum L.)by Agrobacterium-mediated gene transfer.Plant Cell Reports,19(10):989-993.
Kuhl J C,Cheung F,Yuan Q,Martin W,Zewdie Y,Mccallum J,Catanach A,Rutherford P,Sink K C,Jenderek M,Prince J P,Town C D,Havey M J.2004.A unique set of 11008 onion expressed sequence tags reveals expressed sequence and genomic differences between the monocot orders Asparagales and Poales.Plant Cell,16(1):114-125.
Lai Qing-qin,Yin Cheng-hui,Hu You-jia,Yu Mei-jing,Liu Quan-hai.2010.Cloning of alliinase gene from garlic and its expression in Escherichia coli.Chinese Journal of Pharmaceuticals,41(7):496-500.(in Chinese)来庆勤,殷承慧,胡又佳,于美静,刘全海.2010.大蒜蒜氨酸酶基因的克隆及在大肠杆菌中的表达.中国医药工业杂志,41(7):496-500.
Lee H R,Eom E M,Lim Y P,Bang J W,Lee D H.2003.Construction of a garlic BAC library and chromosomal assignment of BAC clones using the FISH technique.Genome,46(3):514-520.
Lee R,Baldwin S,Kenel F,Mccallum J,Macknight R.2013.FLOWERING LOCUS T genes control onion bulb formation and flowering.Nature Communications,4(1):2884.
Liu M,Wu Z,Jiang F.2015a.Selection and validation of garlic reference genes for quantitative real-time PCR normalization.Plant Cell Tissue and Organ Culture,122(2):435-444.
Liu T,Zeng L,Zhu S,Chen X,Tang Q,Mei S,Tang S.2015b.Large-scale development of expressed sequence tag-derived simple sequence repeat markers by deep transcriptome sequencing in garlic(Allium sativum L.).Molecular Breeding,35(11):1-9.
Luo M,Wang Y H,Frisch D,Joobeur T,Wing R A,Dean R A.2001.Melon bacterial artificial chromosome(BAC)library construction using improved methods and identification of clones linked to the locus conferring resistance to melon Fusarium wilt(Fom-2).Genome,44(2):154-162.
Ma K H,Kwag J G,Zhao W,Dixit A,Lee G A,Kim H H,Chung I M,Kim N S,Lee J S,Ji J J,Kim T S,Park Y J.2009.Isolation and characteristics of eight novel polymorphic microsatellite loci from the genome of garlic(Allium sativum L.).Scientia Horticulturae,122(3):355-361.
Maa?H I,Klaas M.1995.Infra-specific differentiation of garlic(Allium sativum L.)by isozyme and RAPD markers.Theoretical and Applied Genetics,91(1):89-97.
Morales R G,Resende J T,Resende F V,Delatorre C A,Figueiredo A S T,Da-Silva P R.2013.Genetic divergence among Brazilian garlic cultivars based on morphological characters and AFLP markers.Genetics and Molecular Research,12(1):270-281.
Neta R,Davidschwartz R,Peretz Y,Sela I,Rabinowitch H D,Flaishman M,Kamenetsky R.2011.Flower development in garlic:the ups and downs of gaLFY expression.Planta,233(5):1063-1072.
Ohri D,Pistrick K.2010.Phenology and genome size variation in Allium L.-a tight correlation?Plant Biology,3(6):654-660.
OvesnáJ,Ku?era L,Horní?kováJ,SvobodováL,StavělíkováH,Velí?ek J,Milella L.2011.Diversity of S-alk(en)yl cysteine sulphoxide content within a collection of garlic(Allium sativum L.)and its association with the morphological and genetic background assessed by AFLP.Scientia Horticulturae,129(4):541-547.
Panda D,Dehury B,Sahu J,Barooah M,Sen P,Modi M K.2014.Computational identification and characterization of conserved miRNAs and their target genes in garlic(Allium sativum L.)expressed sequence tags.Gene,537(2):333-342.
Park M Y,Yi N R,Lee H Y,Kim S T,Kim M,Park J H,Kim J K,Lee J S,Cheong J J,Choi D Y.2002.Generation of chlorsulfuron-resistant transgenic garlic plants(Allium sativum L.)by particle bombardment.Molecular Breeding,9(3):171-181.
Pooler M R,Simon P W.1994.True seed production in garlic.Plant Reproduction,7(5):282-286.
Rabinkov A,Zhu X Z,Grafi G,Galili G,Mirelman D.1994.Alliin lyase(alliinase)from garlic(Allium sativum L.)-biochemical characterization and cDNA cloning.Applied Biochemistry and Biotechnology,48(3):149-171.
Ricroch A,Yockteng R,Brown S C,Nadot S.2005.Evolution of genome size across some cultivated Allium species.Genome,48(3):511-520.
Robert T F,Stephen G R,Robert B H,Stanton B G.1986.Genetic transformation in higher plants.Critical Reviews in Plant Sciences,4(1):1-46.
Rohkin S S,Gillett D,Zemach H,Kimhi S,Forer I,Zutahy Y,Teper-Bamnolker P,Kamenetsky R,Eshel D.2015.Storage temperature controls the timing of garlic bulb formation via shoot apical meristem termination.Planta,242(4):951-962.
Rotem N,Shemesh E,Peretz Y,Akad F,Edelbaum O,Rabinowitch H D,Sela I,Kamenetsky R.2007.Reproductive development and phenotypic differences in garlic are associated with expression and splicing of LEAFY homologue gaLFY.Journal of Experimental Botany,58(5):1133-1141.
Santiago-Valentín E,Francisco-Ortega J.2008.Plant evolution and biodiversity in the Caribbean islands-perspectives from molecular markers.Botanical Review,74(1):1-4.
Sato H,Mizoi J,Tanaka H,Maruyama K,Qin F,Osakabe Y,Morimoto K,Ohori T,Kusakabe K,Nagata M,Shinozaki K,Yamaguchi-Shinozaki K.2014.Arabidopsis DPB3-1,a DREB2A interactor,specifically enhances heat stress-induced gene expression by forming a heat stress-specific transcriptional complex with NF-Y subunits.Plant Cell,26(12):4954-4973.
Shaaf S,Sharma R,Kilian B,Walther A,Ozkan H,Karami E,Mohammadi B.2014.Genetic structure and eco-geographical adaptation of garlic landraces(Allium sativum L.)in Iran.Genetic Resources and Crop Evolution,61(8):1565-1580.
Shimon L J W,Rabinkov A,Shin I,Miron T,Mirelman D,Wilchek M.2007.Two structures of alliinase from Alliium sativum L.:apo form and ternary complex with aminoacrylate reaction intermediate covalently bound to the PLP cofactor.Journal of Molecular Biology,366(2):611-625.
Son J H,Park K C,Lee S I,Kim J H,Kim N S.2012.Species relationships among Allium species by ISSR analysis.Horticulture Environment and Biotechnology,53(3):256-262.
Sun X,Lian H,Liu X,Zhou S,Liu S.2016.The garlic NF-YC gene,AsNF-YC8,positively regulates non-ionic hyperosmotic stress tolerance in tobacco.Protoplasma,254(3):1-14.
Sun X,Zhou S,Meng F,Liu S.2012.De novo assembly and characterization of the garlic(Allium sativum L.)bud transcriptome by Illumina sequencing.Plant Cell Reports,31(10):1823-1828.
Sun Xiu-dong.2013.Transcriptome sequencing in garlic(Allium sativum L.)and functional analysis of the AsNF-YB3[Ph.D.Dissertation].Tai’an:Shandong Agricultural University.(in Chinese)孙秀东.2013.大蒜转录组测序及核因子YB3的功能研究[博士论文].泰安:山东农业大学.
Tang Qiao-ling.2013.Cloning and expression of a novel alliinase gene from garlic roots and selencing of alliinase genes by RNAi[Ph.D.Dissertation].Beijing:Chinese Academy of Agricultural Sciences.(in Chinese)唐巧玲.2013.大蒜根蒜氨酸酶基因的克隆和表达及RNAi沉默研究[博士论文].北京:中国农业科学院.
Tang Qiao-ling,Wang Xu-jing,Wang Zhi-xing.2014.Establishment of particle bombardment-mediated transformation systemin garlic.Journal of Plant Genetic Resources,15(3):576-581.(in Chinese)唐巧玲,王旭静,王志兴.2014.基因枪介导的大蒜遗传转化体系的建立.植物遗传资源学报,15(3):576-581.
Tang Qiao-ling,Wang Zhi-xing,Wang Xu-jing.2015.An alliinase gene and its application[Patent].Beijing:CN104928273A.(in Chinese)唐巧玲,王志兴,王旭静.2015.一种大蒜蒜氨酸酶及其编码基因与应用[专利].北京:CN104928273A.
Trick H N,Finer J J.1997.SAAT:sonication-assisted agrobacterium-mediated transformation.Transgenic Research,6(5):329-336.
van Damme E J,Smeets K,Torrekens S,Van Leuven F,Peumans W J.1992.Isolation and characterization of alliinase cDNA clones from garlic(Allium sativum L.)and related species.European Journal of Biochemistry,209(2):751-757.
Volk G M,Henk A D,Richards C M.2004.Genetic diversity among U.S.garlic clones as detected using AFLP methods.Journal of the American Society for Horticultural Science,129(4):559-569.
Wagner D,Wellmer F,Dilks K,William D,Smith M R,Kumar P P,Riechmann J L,Greenland A J,Meyerowitz E M.2004.Floral induction in tissue culture:a system for the analysis of LEAFY-dependent gene regulation.Plant Journal,39(2):273-282.
Wang H,Li X,Liu X,Oiu Y,Song J,Zhang X,Havey M.2016.Genetic diversity of garlic(Allium sativum L.)germplasm from China by fluorescent-based AFLP,SSR and InDel markers.Plant Breeding,135(6):743-750.
Wang Hai-ping.2011.Evaluation on genetic diversity of garlic germplasm in China and association analysis between allicin content and alliinase genes[Ph.D.Dissertation].Beijing:Chinese Academy of Agricultural Sciences.(in Chinese)王海平.2011.中国大蒜遗传多样性评价及大蒜辣素含量与蒜氨酸酶基因的关联分析[博士论文].北京:中国农业科学院.
Wang M,Wu C,Cheng Z,Meng H.2015.Growth and physiological changes in continuously cropped eggplant(Solanum melongena L.)upon relay intercropping with garlic(Allium sativum L.).Frontiers in Plant Science,6:262.
Weik R,Francky A,Striedner G,Raspor P,Bayer K,Mattanovich D.1998.Recombinant expression of alliin lyase from garlic(Allium sativum)in bacteria and yeasts.Planta Medica,64(4):387-388.
Weiner L,Shin I,Shimon L J,Miron T,Wilchek M,Mirelman D,Frolow F,Rabinkov A.2010.Thiol-disulfide organization in alliin lyase(alliinase)from garlic(Allium sativum L.).Protein Science,18(1):196-205.
Wu C,Wang M,Cheng Z,Meng H.2016a.Response of garlic(Allium sativum L.)bolting and bulbing to temperature and photoperiod treatments.Biology Open,5(4):507-518.
Wu C,Wang M,Dong Y,Cheng Z,Meng H.2016b.Effect of plant age and vernalization on bolting,plant growth and enzyme activity of garlic(Allium sativum L.).Scientia Horticulturae,201:295-305.
Wu Xiao-li,Xu Jian,Zhang Ting,Jia Ping,Zhou Yun-kai.2010.Cloning and sequence analysis of alliinase gene from garlic and construction of Pichia pastoris expression plasmid.Journal of Jilin Agricultural University,32(3):258-263.(in Chinese)吴晓莉,许健,张婷,贾平,周云凯.2010.大蒜蒜氨酸酶基因克隆与序列分析及毕赤酵母表达质粒的构建.吉林农业大学学报,32(3):258-263.
Wu Xiao-li,Zhang Ting,Bian Jin-duo,Xu Jian.2012.Cloning of alliinase gene from garlic bulb and its expression in Pichia pastoris.Chinese Traditional and Herbal Drugs,43(1):143-147.(in Chinese)吴晓莉,张婷,边金铎,许健.2012.大蒜鳞茎蒜氨酸酶基因克隆及其在毕赤酵母中的表达.中草药,43(1):143-147.
Xu Ling,Qiu Xue-mei,Liu Xin-ping,Liu Ren-rong.2012.Research progress of alliance on gene level.Li-Shi-Zhen Medicine Materia Medica Research,23(4):998-1000.(in Chinese)徐玲,裘雪梅,刘新平,刘仁荣.2012.蒜氨酸酶基因水平研究进展.时珍国医国药,23(4):998-1000.
Xu Pei-wen,Yang Chong-liang,Cui De-cai,Qu Shi-song,Liu Bing-jiang,Zhang Jun-ting,Zhang Chuan-kun.2005.A preliminary study on techniques for sexual reproduction of garlic(Allium sativum L.).Acta Horticulturae Sinica,32(3):503-506.(in Chinese)徐培文,杨崇良,崔德才,曲士松,刘冰江,张君亭,张传坤.2005.大蒜有性生殖技术研究初报.园艺学报,32(3):503-506.
Xu Rong,Ren Hong-jie,Wang Hai-zhu,Lou Hu,Xu Qi-jiang.2017.Cloning and expression analysis of MADS-box gene AsPI in garlic.Acta Horticulturae Sinica,44(1):69-79.(in Chinese)徐熔,任洪杰,王海竹,娄虎,徐启江.2017.大蒜MADS-box基因AsPI的克隆及表达分析.园艺学报,44(1):69-79.
Zewdie Y,Havey M J,Prince J P,Jenderek M M.2005.First genetic linkages among expressed regions of the garlic genome.Journal of the American Society for Horticultural Science,130(4):569-574.
Zhang Ai-min,Zhang Su-qin,Sheng Zhong-fei,Liu Li-qing.2009.Extraction and quality analysis of total RNA in different tissues of garlic.Northern Horticulture,(11):108-109.(in Chinese)张爱民,张素勤,盛中飞,刘利青.2009.大蒜不同组织总RNA提取与质量分析.北方园艺,(11):108-109.
Zhang Fen.2008.Evaluation of garlic germplasms based on morphological characters,induction and regeneration of calluses and culture of protoplasts of garlic[Ph.D.Dissertation].Hangzhou:Zhejiang University.(in Chinese)张芬.2008.大蒜种质资源的形态学评价、愈伤诱导再生和原生质体培养[博士论文].杭州:浙江大学.
Zhang Hai-yan.2005.Cloning and functional characterization of heavy metal-responsive genes from garlic[Ph.D.Dissertation].Beijing:Chinese Academy of Science.(in Chinese)张海燕.2005.大蒜重金属抗性基因的克隆及其功能分析[博士论文].北京:中国科学院.
Zheng S J,Henken B,Ahn Y K,Krens F A,Kik C.2004.The development of a reproducible agrobacterium tumefaciens transformation system for garlic(Allium sativum L.)and the production of transgenic garlic resistant to beet armyworm(Spodoptera exigua Hubner).Molecular Breeding,14(3):293-307.
Zhu S,Tang S,Tan Z,Yu Y,Dai Q,Liu T.2017.Comparative transcriptomics provide insight into the morphogenesis and evolution of fistular leaves in Allium.BMC Genomics,18(1):60.