珠子参抗肝损伤药效的物质基础研究
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摘要
目的探索珠子参抗肝损伤药效的物质基础。方法利用溶剂提取法、大孔树脂法及各种色谱技术,进行珠子参有效部位和化学成分的提取分离;采用CCl4所致小鼠化学性肝损伤模型,进行珠子参药效部位及有效成分筛选。将小鼠平均分为对照组、模型组、阳性对照组、珠子参不同剂量组,共9组。每天灌胃1次,连续7d。检测血清中AST和ALT含量的变化。结果珠子参总皂苷高剂量、中剂量可以降低ALT和AST含量,珠子参多糖低剂量组与模型组比较也可以降低ALT和AST的含量,而总皂苷高剂量有显著作用;竹节参皂苷Ⅳa各剂量组、人参皂苷Ro中、低剂量组均能降低ALT和AST的含量,与模型组对比,差异有统计学意义,竹节参皂苷Ⅳa低剂量效果显著。结论珠子参总皂苷为珠子参抗肝损伤的有效部位,主要化学成分竹节参皂苷Ⅳa为其抗肝损伤的主要有效成分。
Objective To study the therapeutic material basis of Panax japonicus in anti-hepatic injury.Methods Chemical compositions of Panax japonicus were extracted and separated by macroporous resin column chromatography.The active ingredients were screened by using the mice model of acute liver injury.The mice were divided into nine groups,normal control,model group,positive control,the total saponins and polysaccharide of Panax japonicus group with high,medium and low-dose.The activities of ALT,AST were examined after the administration of drugs orally for 7days.Results Compared with the model control group,the total saponins with high and medium dose and polysaccharide of Panax japonicus group with low-dose could significantly decrease the level of ALT,AST;chikusetsusaponinⅣa all-dose group and ginsenoside Ro with medium and low dose group could significantly decrease the concentration of ALT,AST.Conclusion The total saponins and chikusetsusaponinⅣ a were effective parts and active ingredients for anti-hepatic injury.
Objective To study the therapeutic material basis of Panax japonicus in anti-hepatic injury.Methods Chemical compositions of Panax japonicus were extracted and separated by macroporous resin column chromatography.The active ingredients were screened by using the mice model of acute liver injury.The mice were divided into nine groups,normal control,model group,positive control,the total saponins and polysaccharide of Panax japonicus group with high,medium and low-dose.The activities of ALT,AST were examined after the administration of drugs orally for 7days.Results Compared with the model control group,the total saponins with high and medium dose and polysaccharide of Panax japonicus group with low-dose could significantly decrease the level of ALT,AST;chikusetsusaponinⅣa all-dose group and ginsenoside Ro with medium and low dose group could significantly decrease the concentration of ALT,AST.Conclusion The total saponins and chikusetsusaponinⅣ a were effective parts and active ingredients for anti-hepatic injury.
引文
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[2]国家药典委员会.中国药典2010年版[S].一部.北京:中国医药科技出版社,2010:254-255.
[3]宋小妹,刘越,蔡宝昌.珠子参的化学成分[J].沈阳药科大学学报,2010,27(8):626-630.
[4]考玉萍,姜祎,宋小妹.珠子参叶总皂苷抗疲劳抗应激作用的实验研究[J].陕西中医,2008,29(8):1092-1093.
[5]姜祎,考玉萍,宋小妹.珠子参叶总皂苷抗炎镇痛作用的实验研究[J].陕西中医,2008,29(6):732-733.
[6]高培红,曹军毅,宋小妹.正交实验法优选珠子参总皂苷的提取工艺[J].陕西中医学院学报,2006,29(2):66-67.
[7]王薇,高培红,康亚国,等.大孔吸附树脂富集纯化珠子参皂苷的工艺研究[J].辽宁中医杂志,2010,37(4):713-715.
[8]袁菊丽,房方,张旋,等.珠子参多糖提取纯化工艺研究[J].河南中医,2007,27(12):73-74.
[9]宋小妹,李伟东,房方,等.珠子参药材质量标准研究[J].南京中医药大学学报,2010,26(2):143-145.
[10]王虹,刘红梅,王磊.迷迭香酸对四氯化碳致小鼠急性肝损伤的保护作用[J].中成药,2009,31(3):354-358.
[11]赵军,刘涛,马龙,等.类叶升麻苷对大鼠原代肝细胞免疫性肝损伤的保护作用[J].西北药学杂志,2013,28(3):269-271.
[12]郭冬艳,覃鸿恩,李瑾,等.太白楤木抗肝损伤作用药效物质基础的初步研究[J].医学研究杂志,2012,41(10):69-71.
[13]姜辉,高家荣,张家富,等.三七总皂苷对肝纤维化大鼠的保护作用及机制[J].中药药理与临床,2013,29(3):71-74.