五味子含药血清中木脂素类成分的UPLC-MS/MS分析
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摘要
目的分析五味子含药血清中的木脂素类成分。方法 UPLC-MS/MS检测,经与五味子提取物、空白血清总离子流色谱图比较,发现含药血清中的药源性成分;经与对照品的保留时间和质谱数据相比对,对部分原形成分进行鉴定;经与提取物相同保留时间色谱峰质谱数据比较并参考相关文献,对部分原形成分进行推测。结果大鼠灌胃给予五味子提取物后,含药血清中产生了药源性成分,包括代谢产物和原形成分。鉴定了其中4个原形成分:五味子醇甲、五味子醇乙、五味子甲素和五味子乙素;推测了另外10个原形成分:戈米辛D、巴豆酰戈米辛H、当归酰戈米辛H、当归酰戈米辛P、五味子酯乙、五味子酚、戈米辛O、戈米辛N、6-O-苯甲酰戈米辛O和五味子丙素,所有14个成分均为联苯环辛烯类木脂素。结论五味子含药血清中的药源性成分主要为联苯环辛烯类木脂素。
Objective To analyze lignans in serum containing drug(SCD) of Schisandra chinensis.Methods Samples was detected by UPLC-MS/MS.By comparing the total ion current(TIC) chromatogram of SCD with the ones of extract of Schisandra chinensis and blank serum,drug-induced constituents were found.Some original compounds were identified by comparing the retention time and mass data with reference compounds and some other ones were suspected by comparing the mass data of SCD with the ones of extract of Schisandra chinensis and consultting literatures.Results Drug-induced constituents were found in rat's serum after oral administration of Schisandra chinensis,including original compounds contained in materia medica and their metabolites.Four original compounds in SCD were identified as schisandrin,schisandrol B,deoxyschisandrin,schisandrin B,and ten other ones were suspected as gomisin D,tigloylgomisin H,angeloylgomisin H,angeloylgomisin P,schisantherin B,schisanhenol,gomisin O,gomisin N,6-O-benzoylgomisin O,and schisandrin C.All fourteen ones were dibenzocyclooctadiene lignans.Conclusion Main drug-induced constituents in SCD of Schisandra chinensis were dibenzocyclooctadiene lignans.
Objective To analyze lignans in serum containing drug(SCD) of Schisandra chinensis.Methods Samples was detected by UPLC-MS/MS.By comparing the total ion current(TIC) chromatogram of SCD with the ones of extract of Schisandra chinensis and blank serum,drug-induced constituents were found.Some original compounds were identified by comparing the retention time and mass data with reference compounds and some other ones were suspected by comparing the mass data of SCD with the ones of extract of Schisandra chinensis and consultting literatures.Results Drug-induced constituents were found in rat's serum after oral administration of Schisandra chinensis,including original compounds contained in materia medica and their metabolites.Four original compounds in SCD were identified as schisandrin,schisandrol B,deoxyschisandrin,schisandrin B,and ten other ones were suspected as gomisin D,tigloylgomisin H,angeloylgomisin H,angeloylgomisin P,schisantherin B,schisanhenol,gomisin O,gomisin N,6-O-benzoylgomisin O,and schisandrin C.All fourteen ones were dibenzocyclooctadiene lignans.Conclusion Main drug-induced constituents in SCD of Schisandra chinensis were dibenzocyclooctadiene lignans.
引文
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[2]窦志华,丁安伟,王陆军,等.复方五仁醇胶囊血清药化学研究[J].中草药,2006;37:1137-1140.
[3]丁安伟,窦志华,罗琳,等.复方五仁醇胶囊血清药化学研究(Ⅱ)[J].中草药,2008;39:1285-1288.
[4]刘海涛,齐耀东,许利嘉,等.中国五味子科植物传统药物学调查[J].中国中药杂志,2012;37:1353-1359.
[5]皮子凤,侯广月,艾军,等.化学计量学方法研究北五味子中木脂素含量与抗氧化活性的相关性[J].中国中药杂志,2012;37:1133-1139.
[6]史琳,何晓霞,潘英,等.五味子藤茎化学成分的研究[J].中草药,2009;40:1707-1710.
[7]窦志华,罗琳,丁安伟,等.复方五仁醇胶囊含药血清指纹图谱与保肝作用的谱效关系[J].第四军医大学学报,2008;29:116-118.
[8]薛雪,张红芬,白小红,等.超分子有序聚集凝固液相微萃取及其对五味子中木脂素类化合物的测定及质量评价[J].高等学校化学学报,2012;33:942-947.
[9]吴陈军,窦志华,李伟红,等.正交试验法优选复方五仁醇胶囊中间体五仁醇浸膏提取工艺研究[J].齐鲁药事,2007;26:430-431.
[10]史培颖.中药四类成分质谱裂解规律及芪参益气方药代动力学研究[D].杭州:浙江大学,2010.
[11]王宝莲.中药五味子成分的药代动力学及代谢研究[D].北京:中国协和医科大学,2008.
[12]沈振铎.五味子质量控制方法研究[D].沈阳:沈阳药科大学,2008.
[13]黄鑫,宋凤瑞,刘志强,等.五味子中木脂素类成分的高效液相色谱-电喷雾质谱研究[J].化学学报,2008;66:1059-1066.
[14]王薇丹,叶正良,宋丽丽,等.五味子液相色谱指纹图谱的研究[J].中国中药杂志,2010;35:2871-2873.