维生素E和裂壶藻对斑节对虾精荚再生的协同作用
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摘要
文章探究了维生素E(Vitamin E,V_E)和裂壶藻(Schizochytrium sp.,Sch)对斑节对虾(Penaeus monodon)雄虾精荚再生的作用。以基础饲料配制了4种不同配合饲料(0添加组;V_E 200组;1%Sch组;V_E 200+1%Sch组),饲喂被挤压排出精荚的成熟雄性斑节对虾40 d,评估斑节对虾雄虾精荚再生后的精子数量和质量。结果表明,添加量为200 mg·kg~(-1) V_E饲料组的活精子数百分比显著高于对照组(P<0.05);单一添加1%裂壶藻饲料组的活精子数与对照组间差异不显著(P>0.05);共同添加200 mg·kg~(-1) V_E和1%裂壶藻的饲料组的精子总数显著高于对照组(P<0.05)。综合精荚质量、精子数量及活精子数百分比3个指标得出,饲料中共同添加1%裂壶藻与200 mg·kg~(-1) V_E对斑节对虾雄虾精荚的再生和提高精子数量具有协同促进作用。
To investigate the effects of Vitamin E and Schizochytrium sp. on the spermatophore regeneration in Penaeus monodon,we designed four different contents of Vitamin E and Schizochytrium sp. in the basal diet(control group; V_E 200; 1% Schizochytrium sp.; V_E 200+1% Schizochytrium sp.) to feed mature male P.monodon which had been extruded spermatophore for 40 d. The results show that the percentage of viable spermatozoa added in 200 mg·kg~(-1) Vitamin E diet group was significantly higher than that in the control group(P<0.05). The sperm quantity of single added in 1% Schizochytrium sp. diet group was not significantly different with control group(P>0.05). The total number of spermatozoa fed with 200 mg·kg~(-1) of Vitamin E and 1% Schizochytrium sp. was significantly higher than that of the control group(P<0.05). According to the spermatophore quality, sperm quantity and living sperm percentage, adding 200 mg·kg~(-1) of Vitamin E and 1% Schizochytrium sp. in feed can promote the spermatophore regeneration in the male P.monodon and improve the sperm quantity effectively.
To investigate the effects of Vitamin E and Schizochytrium sp. on the spermatophore regeneration in Penaeus monodon,we designed four different contents of Vitamin E and Schizochytrium sp. in the basal diet(control group; V_E 200; 1% Schizochytrium sp.; V_E 200+1% Schizochytrium sp.) to feed mature male P.monodon which had been extruded spermatophore for 40 d. The results show that the percentage of viable spermatozoa added in 200 mg·kg~(-1) Vitamin E diet group was significantly higher than that in the control group(P<0.05). The sperm quantity of single added in 1% Schizochytrium sp. diet group was not significantly different with control group(P>0.05). The total number of spermatozoa fed with 200 mg·kg~(-1) of Vitamin E and 1% Schizochytrium sp. was significantly higher than that of the control group(P<0.05). According to the spermatophore quality, sperm quantity and living sperm percentage, adding 200 mg·kg~(-1) of Vitamin E and 1% Schizochytrium sp. in feed can promote the spermatophore regeneration in the male P.monodon and improve the sperm quantity effectively.
引文
[1]江世贵,杨丛海,周发林,等.斑节对虾种虾繁育技术[M].北京:海洋出版社,2013:5-109.
[2]杨丽诗,黄建华,孙苗苗,等.3种来源的野生斑节对虾携带病毒情况调查[J].上海海洋大学学报,2011,20(4):546-552.
[3]江世贵,何建国,吕玲,等.白斑综合症病毒对斑节对虾亲虾的感染及垂直传播的初步研究[J].中山大学学报(自然科学版),2000,39(z1):164-171.
[4]黄建华,马之明,周发林,等.南海北部野生斑节对虾卵巢解剖结构及组织学的研究[J].南方水产,2005,1(3):49-53.
[5]黄建华,杨其彬,马之明,等.池塘养殖斑节对虾的生长、发育与性成熟[J].水产学报,2013,37(3):397-406.
[6]黄建华,马之明,周发林,等.池塘养殖斑节对虾的生长特性[J].海洋水产研究,2006,27(1):14-20.
[7]洪水根,李祺福,郭永刚,等.斑节对虾精子发生的超微结构[J].动物学报,1998,44(1):1-4.
[8]杜少波,胡超群,沈琪.亲虾营养需求研究进展[J].热带海洋学报,2002,21(4):80-91.
[9]WOUTERS R,LAVENS P,NIETO J,et al.Penaeid shrimp broodstock nutrition:an updated review on research and development[J].Aquaculture,2001,202(1/2):1-21.
[10]LYTLE J S,LYTLE T F,OGLE J T.Polyunsaturated fatty acid profiles as a comparative tool in assessing maturation diets of Penaeus vannamei[J].Aquaculture,1990,89(3/4):287-299.
[11]周发林,马之明,黄建华,等.4种斑节对虾亲虾饵料蛋白质的营养价值评价[J].广东海洋大学学报,2005,25(4):9-13.
[12]LEE M H,SHIAU S Y.Vitamin E requirements of juvenile grass shrimp,Penaeus monodon,and effects on non-specific immune responses[J].Fish Shellfish Immunol,2004,16(4):475-485.
[13]CAHU C L,CUZON G,QUAZUGUEL P.Effect of highly unsaturated fatty acids,α-tocopherol and ascorbic acid in broodstock diet on egg composition and development of Penaeus indicus[J].Comp Biochem Physiol A,1995,112(3/4):417-424.
[14]胡文琴,孟庆利,王恬.维生素E对畜禽繁殖性能的影响[J].当代畜禽养殖业,2004(5):15-16.
[15]WOUTERS R,GOMEZ L,LAVENS P,et al.Feeding enriched Artemia biomass to Penaeus vannamei broodstock:its effect on reproductive performance and larval quality[J].J Shellfish Res,1999,18(2):651-656.
[16]李浩洋,班甲,陈骏佳,等.日粮添加裂壶藻对鸡蛋DHA含量、品质及蛋鸡生产性能的影响[J].中国饲料,2015(11):37-39,42.
[17]陈家鑫.裂壶藻及其制品在水产苗种培育中的应用[J].科学养鱼,2002(6):53.
[18]黄建华,周发林,林黑着,等.池塘养殖斑节对虾卵巢发育过程中的脂肪酸组成及变化[J].上海水产大学学报,2007,16(4):341-346.
[19]洪心.人工移植精荚技术在斑节对虾育苗中的应用[J].福建水产,2012,34(4):331-334.
[20]LEROY J L,van HOECK V,CLEMENTE M,et al.The effect of nutritionally induced hyperlipidaemia on in vitro bovine embryo quality[J].Hum Reprod,2010,25(3):768-778.
[21]赵连翠,蔡生力.对虾精荚和精子的研究进展[J].海洋科学,2005,29(2):73-77.
[22]李振.维生素E在水产养殖中的应用[J].渔业现代化,2006(5):35-36.
[23]张国辉,何瑞国,张世萍,等.维生素E对黄鳝繁殖性能的影响[J].华中农业大学学报,2006,25(2):177-181.
[24]冯伟.维生素C、E和裂壶藻对中国对虾非特异性免疫功能影响的研究[D].上海:上海海洋大学,2011:45-68.
[25]李美玉,李健,陈萍,等.维生素E和裂壶藻对中国对虾生长及TLR/NF-κB表达水平的影响[J].中国渔业质量与标准,2012,2(2):37-44.
[26]王桂芹,牛小天,闫先春,等.饲料中添加维生素E和二十二碳六烯酸对鲤鱼生长和抗病力的影响[J].上海交通大学学报(农业科学版),2010,28(2):124-128.
[27]LEIBOVITZ B E,HU M L,TAPPEL A L.Lipid peroxidation in rat tissue slices:effect of dietary vitamin E,corn oil-lard and menhaden oil[J].Lipids,1990,25(3):125-129.
[28]HU M L,FRANKEL E N,LEIBOVITZ B E,et al.Effect of dietary lipids and vitamin E on in vitro lipid peroxidation in rat liver and kidney homogenates[J].J Nutr,1989,119(11):1574-1582.
[29]BART A N,CHOOSUK S,THAKUR D P.Spermatophore cryopreservation and artificial insemination of black tiger shrimp,Penaeus monodon(Fabricius)[J].Aquacult Res,2006,37(5):523-528.
[30]GUPTA P S P,RAO L H.Spermatophore regeneration time in Penaeus monodon[J].Indian J Anim Sci,2000,70(7):780-781.
[31]SAMPATH K,RAMACHANDRAN T.Spermatophore collection in Penaeus monodon by electrical stimulation[J].J Aquacult Trop,1999(14):193-199.
[32]林莉,姚东明,林琼武,等.日本囊对虾精荚再生能力的研究[J].厦门大学学报(自然科学版),2014,53(2):283-288.
[33]袁路,蔡生力.温度、盐度对凡纳滨对虾精荚再生和精子质量的影响[J].水产学报,2006,30(1):63-68.
[34]JIANG S G,HUANG J H,ZHOU F L,et al.Observations of reproductive development and maturation of male Penaeus monodon reared in tidal and earthen ponds[J].Aquaculture,2009,292(1/2):121-128.
[2]杨丽诗,黄建华,孙苗苗,等.3种来源的野生斑节对虾携带病毒情况调查[J].上海海洋大学学报,2011,20(4):546-552.
[3]江世贵,何建国,吕玲,等.白斑综合症病毒对斑节对虾亲虾的感染及垂直传播的初步研究[J].中山大学学报(自然科学版),2000,39(z1):164-171.
[4]黄建华,马之明,周发林,等.南海北部野生斑节对虾卵巢解剖结构及组织学的研究[J].南方水产,2005,1(3):49-53.
[5]黄建华,杨其彬,马之明,等.池塘养殖斑节对虾的生长、发育与性成熟[J].水产学报,2013,37(3):397-406.
[6]黄建华,马之明,周发林,等.池塘养殖斑节对虾的生长特性[J].海洋水产研究,2006,27(1):14-20.
[7]洪水根,李祺福,郭永刚,等.斑节对虾精子发生的超微结构[J].动物学报,1998,44(1):1-4.
[8]杜少波,胡超群,沈琪.亲虾营养需求研究进展[J].热带海洋学报,2002,21(4):80-91.
[9]WOUTERS R,LAVENS P,NIETO J,et al.Penaeid shrimp broodstock nutrition:an updated review on research and development[J].Aquaculture,2001,202(1/2):1-21.
[10]LYTLE J S,LYTLE T F,OGLE J T.Polyunsaturated fatty acid profiles as a comparative tool in assessing maturation diets of Penaeus vannamei[J].Aquaculture,1990,89(3/4):287-299.
[11]周发林,马之明,黄建华,等.4种斑节对虾亲虾饵料蛋白质的营养价值评价[J].广东海洋大学学报,2005,25(4):9-13.
[12]LEE M H,SHIAU S Y.Vitamin E requirements of juvenile grass shrimp,Penaeus monodon,and effects on non-specific immune responses[J].Fish Shellfish Immunol,2004,16(4):475-485.
[13]CAHU C L,CUZON G,QUAZUGUEL P.Effect of highly unsaturated fatty acids,α-tocopherol and ascorbic acid in broodstock diet on egg composition and development of Penaeus indicus[J].Comp Biochem Physiol A,1995,112(3/4):417-424.
[14]胡文琴,孟庆利,王恬.维生素E对畜禽繁殖性能的影响[J].当代畜禽养殖业,2004(5):15-16.
[15]WOUTERS R,GOMEZ L,LAVENS P,et al.Feeding enriched Artemia biomass to Penaeus vannamei broodstock:its effect on reproductive performance and larval quality[J].J Shellfish Res,1999,18(2):651-656.
[16]李浩洋,班甲,陈骏佳,等.日粮添加裂壶藻对鸡蛋DHA含量、品质及蛋鸡生产性能的影响[J].中国饲料,2015(11):37-39,42.
[17]陈家鑫.裂壶藻及其制品在水产苗种培育中的应用[J].科学养鱼,2002(6):53.
[18]黄建华,周发林,林黑着,等.池塘养殖斑节对虾卵巢发育过程中的脂肪酸组成及变化[J].上海水产大学学报,2007,16(4):341-346.
[19]洪心.人工移植精荚技术在斑节对虾育苗中的应用[J].福建水产,2012,34(4):331-334.
[20]LEROY J L,van HOECK V,CLEMENTE M,et al.The effect of nutritionally induced hyperlipidaemia on in vitro bovine embryo quality[J].Hum Reprod,2010,25(3):768-778.
[21]赵连翠,蔡生力.对虾精荚和精子的研究进展[J].海洋科学,2005,29(2):73-77.
[22]李振.维生素E在水产养殖中的应用[J].渔业现代化,2006(5):35-36.
[23]张国辉,何瑞国,张世萍,等.维生素E对黄鳝繁殖性能的影响[J].华中农业大学学报,2006,25(2):177-181.
[24]冯伟.维生素C、E和裂壶藻对中国对虾非特异性免疫功能影响的研究[D].上海:上海海洋大学,2011:45-68.
[25]李美玉,李健,陈萍,等.维生素E和裂壶藻对中国对虾生长及TLR/NF-κB表达水平的影响[J].中国渔业质量与标准,2012,2(2):37-44.
[26]王桂芹,牛小天,闫先春,等.饲料中添加维生素E和二十二碳六烯酸对鲤鱼生长和抗病力的影响[J].上海交通大学学报(农业科学版),2010,28(2):124-128.
[27]LEIBOVITZ B E,HU M L,TAPPEL A L.Lipid peroxidation in rat tissue slices:effect of dietary vitamin E,corn oil-lard and menhaden oil[J].Lipids,1990,25(3):125-129.
[28]HU M L,FRANKEL E N,LEIBOVITZ B E,et al.Effect of dietary lipids and vitamin E on in vitro lipid peroxidation in rat liver and kidney homogenates[J].J Nutr,1989,119(11):1574-1582.
[29]BART A N,CHOOSUK S,THAKUR D P.Spermatophore cryopreservation and artificial insemination of black tiger shrimp,Penaeus monodon(Fabricius)[J].Aquacult Res,2006,37(5):523-528.
[30]GUPTA P S P,RAO L H.Spermatophore regeneration time in Penaeus monodon[J].Indian J Anim Sci,2000,70(7):780-781.
[31]SAMPATH K,RAMACHANDRAN T.Spermatophore collection in Penaeus monodon by electrical stimulation[J].J Aquacult Trop,1999(14):193-199.
[32]林莉,姚东明,林琼武,等.日本囊对虾精荚再生能力的研究[J].厦门大学学报(自然科学版),2014,53(2):283-288.
[33]袁路,蔡生力.温度、盐度对凡纳滨对虾精荚再生和精子质量的影响[J].水产学报,2006,30(1):63-68.
[34]JIANG S G,HUANG J H,ZHOU F L,et al.Observations of reproductive development and maturation of male Penaeus monodon reared in tidal and earthen ponds[J].Aquaculture,2009,292(1/2):121-128.