纳米氧化铈对48h睡眠剥夺小鼠生精能力的影响
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摘要
为了研究纳米氧化铈(cerium oxide nanoparticles, CeO2NPs)对48 h睡眠剥夺小鼠生精能力的影响,并探讨其作用机制。将36只6周龄雄性健康清洁级ICR小鼠分为6组:空白对照组、溶剂对照组、睡眠剥夺对照组、CeO2NPs低、中、高剂量组。CeO2NPs低、中、高剂量组分别以4 mg/kg、8 mg/kg和16 mg/kg灌胃1 m L的纳米氧化铈溶液,溶剂对照组和睡眠剥夺对照组灌胃等量溶剂,空白对照组灌胃等量蒸馏水,连续30 d。第31天,采用单平台水环境法进行48 h睡眠剥夺。继之,测定小鼠每日精子生成量、睾丸组织内标志酶(G6PDH,LDH和ACP)及抗氧化指标(CAT, MDA和T-AOC)。与溶剂对照组比较,48 h睡眠剥夺使小鼠睾丸每日精子生成量降低,睾丸标志酶G6PDH、ACP和LDH活力下调,睾丸组织CAT活力和T-AOC水平降低,小鼠睾丸MDA含量升高。与睡眠剥夺对照组比较,CeO2NPs低、中、高剂量组均改善了48 h睡眠剥夺小鼠的每日精子生成量,其中中剂量组的改善更为明显,且在调节睡眠剥夺小鼠睾丸标志酶G6DPH、LDH和ACP活力、提高睾丸组织内CAT活力、T-AOC水平及降低MDA含量方面最为显著。纳米氧化铈可以改善睡眠剥夺雄性小鼠的生精能力,这可能与提高了睾丸组织的抗氧化能力,从而改善了氧化应激损伤并调节了能量消耗代谢有关。
To study the effects of cerium oxide nanoparticles(CeO2 NPs) on spermatogenic function in 48 hours of sleep deprived male mice and explore its mechanism. Thirty-six healthy clean ICR male mice(six weeks old) were randomly divided into 6 groups: blank control group, solvent control group, sleep deprivation control group, low,medium and high dose groups of CeO2 NPs. 1 m L CeO2 NPs solvent(4 mg/kg, 8 mg/kg and 16 mg/kg) was administered to mice of low, medium and high dose groups of CeO2 NPs, respectively. Solvent control and sleep deprivation control group were given with equivalent solvent, Blank group was given with equivalent distilled water. Intragastric administration continued for 30 days. On the 31 st day, a single platform water environment method was used for48 hours of sleep deprivation on mice. For every animal, daily sperm production, marker enzymes of testis(G6 PDH,LDH, ACP) and the antioxidant of testis tissue(CAT, MDA, T-AOC) were measured. Compared with the solvent con trol group, 48 h sleep deprivation reduced daily sperm production of mice, the activities of G6 PDH, ACP,LDH, CAT, T-AOC level, but increased the content of MDA in testis tissue. Compared with the sleep deprivation control group, the low, medium and high dose groups of CeO2 NPs improved the daily sperm production of 48 h sleep deprived mice. In three dose groups, the middle dose group had the best effect in curbing the adverse impacts of sleep deprivation on daily sperm production and the activity of G6 DPH, LDH and ACP in testis. At the same time, the middle dose has the most significant effect in improving the level of T-AOC in testis and decreasing the content of MDA. CeO2 NPs can improve the spermatogenic function of sleep deprived male mice, the mechanism of which might be involved in increasing antioxidant function,decreasing the damage of free radicals on testis and adjusting the energy metabolism of testis.
To study the effects of cerium oxide nanoparticles(CeO2 NPs) on spermatogenic function in 48 hours of sleep deprived male mice and explore its mechanism. Thirty-six healthy clean ICR male mice(six weeks old) were randomly divided into 6 groups: blank control group, solvent control group, sleep deprivation control group, low,medium and high dose groups of CeO2 NPs. 1 m L CeO2 NPs solvent(4 mg/kg, 8 mg/kg and 16 mg/kg) was administered to mice of low, medium and high dose groups of CeO2 NPs, respectively. Solvent control and sleep deprivation control group were given with equivalent solvent, Blank group was given with equivalent distilled water. Intragastric administration continued for 30 days. On the 31 st day, a single platform water environment method was used for48 hours of sleep deprivation on mice. For every animal, daily sperm production, marker enzymes of testis(G6 PDH,LDH, ACP) and the antioxidant of testis tissue(CAT, MDA, T-AOC) were measured. Compared with the solvent con trol group, 48 h sleep deprivation reduced daily sperm production of mice, the activities of G6 PDH, ACP,LDH, CAT, T-AOC level, but increased the content of MDA in testis tissue. Compared with the sleep deprivation control group, the low, medium and high dose groups of CeO2 NPs improved the daily sperm production of 48 h sleep deprived mice. In three dose groups, the middle dose group had the best effect in curbing the adverse impacts of sleep deprivation on daily sperm production and the activity of G6 DPH, LDH and ACP in testis. At the same time, the middle dose has the most significant effect in improving the level of T-AOC in testis and decreasing the content of MDA. CeO2 NPs can improve the spermatogenic function of sleep deprived male mice, the mechanism of which might be involved in increasing antioxidant function,decreasing the damage of free radicals on testis and adjusting the energy metabolism of testis.
引文
Choi J.H.,Lee S.H.,Bae J.H.,Shim J.S.,Park H.S.,Kim Y.S.,and Shin C.,2016,Effect of sleep deprivation on the male reproductive system in rats,J.Korean Med.Sci.,31(10):1624-1630
Gao Y.,Huang Z.F.,Yu H.B.,Huang X.F.,Zhou Y.Y.,and Ma C.,2015,The effect of nano ceria particles pretreatment on oxidative damage of rat myocardial cells,Zhonghua Shiyan Waike Zazhi(Chinese Journal of Experimental Surgery),32(5):1093-1096(高勇,法宪恩,黄真锋,余海彬,周玉阳,马超,2015,纳米二氧化铈颗粒预处理对大鼠心肌细胞氧化损伤的影响,中华实验外科杂志,32(5):1093-1096)
Jiang C.,Zhou R.,Xia C.C.,Feng J.,Li F.G.,Huang Z.Y.,and Cui T.,2013,72 h sleep deprivation induced reproductive toxicity in male mice and its mechanism,Xiandai Yufang Yixue(Modern Preventive Medicine),40(7):1325-1326,1329(蒋超,周冉,夏聪聪,冯健,李丰功,黄振遥,崔涛,2013,72 h睡眠剥夺对雄性小鼠的生殖毒性效应及机制,现代预防医学,40(7):1325-1326,1329)
Kheradmand A.,Alirezaei M.,Asadian P.,Alavi E.R.,and Joorabi S.,2009,Antioxidant enzyme activity and MDA level in the rat testis following chronic administration of ghrelin,Andrologia,41(6):335-340
Liu C.L.,Xu X.,Ha L.X.,Pei L.G.,Jing W.H.,and Wu X.,2015,To study the effect of catalase on oxidative damage in testis of mice,Ningxia Yixue Zazhi(Ningxia Medical Journal),37(9):783-784(刘春莲,徐仙,哈灵侠,裴利国,景万红,吴昕,2015,过氧化氢酶对小鼠睾丸氧化损伤的作用研究,宁夏医学杂志,37(9):783-784)
Liu M.M.,Liu L.,Chen L.,Yin X.J.,Liu H.,Zhang Y.H.,Li P.L.,Wang S.,Li X.X.,and Yu C.H.,2017,Sleep deprivation and late bedtime impair sperm health through increasing antisperm antibody production:a prospective study of 981 healthy men,Med.Sci.Monit.,23:1842-1848
Lv G.M.,Wang Y.J.,Liu R.,Sun L.D.,Huang Y.H.,and Yan C.H.,2013,Biological application of nano cerium oxide,Zhongguo Kexue(Chinese Science:Chemistry),43(10):1309-1321(吕广明,王艳杰,刘瑞,孙聆东,黄云辉,严纯华,2013,纳米氧化铈的抗氧化生物应用,中国科学:化学,43(10):1309-1321)
Rzigalinski B.A.,Carfagna C.S.,and Ehrich M.,2017,Cerium oxide nanoparticles in neuroprotection and considerations for efficacy and safety,Wiley Interdiscip.Rev.Nanomed.Nanobiotechnol.,9(4):10.1002
Shen Y.N.,Guo J.L.,and Liu A.Q.,2013,Cerium oxide and life,Xitu(Rare Earth),34(1):77-80(沈岳年,郭金玲,刘阿秋,2013,纳米氧化铈和生命,稀土,34(1):77-80)
Sinha N.,Narayan R.,and Saxena D.K.,1997,Effect of endosulfan on the testis of growing rats,Bull Environ Contam Toxicol,58(1):79-86
Song B.,and Francis C.,2008,The relationship between androgen and spermatogenesis,Linchuang Miniao Waike Zazhi(Journal of Clinical Department of Urology),23(7):553-555(宋博,蔡志明,2008,雄激素与精子发生的关系,临床泌尿外科杂志,23(7):553-555)
Tobaldini E.,Costantino G.,Solbiati M.,Cogliati C.,Kara T.,Nobili L.,and Montano N.,2016,Sleep,sleep deprivation,autonomic nervous system and cardiovascular diseases,Neurosci.and Biobehav.Rev.,74(Pt B):321-329
Touitou Y.,Reinberg A.,and Touitou D.,2017,Association between light at night,melatonin secretion,sleep deprivation,and the internal clock:health impacts and mechanisms of circadian disruption,Life Sci.,173:94-106
Wu J.L.,Wu S.C.,Yang J.G.,Huang C.C.,Chen K.B.,Fang K.H.,and Tsai H.D.,2011,Effects of sleep deprivation on serum testosterone concentrations in the rat,Neurosci.Lett.,494(2):124
Wu T.,Zhang Z.Q.,Ji L.,Yang T.W.,Zhang B.W.,and Wu G.,2017,Effect of nano cerium oxide on oxidative damage of alveolar macrophages induced by hydrogen peroxide,Huanjing Yu Jiankang Zazhi(Journal of Environment and Health),34(1):9-12(吴涛,张志强,纪乐,杨通旺,张博闻,吴刚,2017,纳米氧化铈对过氧化氢所致大鼠肺泡巨噬细胞氧化损伤的影响,环境与健康杂志,34(1):9-12)
Xie J.R.,Cheng L.L.,Zhu Z.Y.,and Ding Z.Z.,2016,Progress in the study of sleep disorder on reproductive effects,Guoji Shengzhi Jiankang,Jihua Shengyu Zazhi(International Journal of Reproductive Health,Family Planning),35(1):78-82(谢金容,程丽霖,朱真仪,丁之德,2016,睡眠障碍对生殖系统影响的研究进展,国际生殖健康,计划生育杂志,35(1):78-82)
Xu F.,Yang N.,Liang Y.H.,Zheng M.W.,Wang Z.H.,He Q.J.,Shen Y.W.,Li H.Z.,Wang X.Q.,and Lin L.X.,2017,Deep slow wave sleep deprivation effects on oxidative stress in rat testis,Zhonghua Nankexue Zazhi(The Journal of Science),23(8):687-691(徐飞,杨娜,梁钰涵,何其瑾,郑美雯,汪子涵,沈奕薇,李海征,王雪倩,林来祥,2017,深慢波睡眠剥夺对大鼠睾丸组织氧化应激反应的影响,中华男科学杂志,23(8):687-691)
Xu L.C.,Wang S.Y.,and Wang X.R.,2000,Enzymatic study of cadmium toxicity to testis in rats,Zhongguo Gonggong Weisheng(Public Health of China),16(2):119-120(徐莉春,王沭沂,王心如,2000,镉对大鼠睾丸毒作用的酶学研究,中国公共卫生,16(2):119-120)
Yu D.L.,and Wang C.L.,2016,Research progress of catalase,Zhongguo Zuzhi Huaxue Yu Xibao Huaxue Zazhi(Chinese Journal of Histochemistry and Cytochemistry),25(2):189-194(于德玲,王昌留,2016,过氧化氢酶的研究进展,中国组织化学与细胞化学杂志,25(2):189-194)
Zamanian Z.,Nikeghbal K.,and Khajehnasiri F.,2016,Influence of sleep on quality of life among hospital nurses,Electron.Physician,8(1):1811-1816
Zhao Y.F.,Ren F.Q.,Hu K.N.,and Chang D.G.,2015,Sleep deprivation may investigate the mechanism of abnormal spermatozoa,Heilongjiang Yixue(Heilongjiang Medical),10:1165-1166(赵一帆,任飞强,胡开妮,常德贵,2015,睡眠剥夺导致精子质量异常的可能机制探讨,黑龙江医学,10:1165-1166)
Zhou D.S.,Zhou J.,Zheng C.,Long D.B.,Zhou Y.J.,Tang H.Y.,Liu C.,Zhang Y.Z.,Wang X.,and Deng X.X.,2014,The effects of sleep deprivation on body weight and sexual behavior of male mice,Heilongjiang Xumu Shouyi(Heilongjiang Animal Husbandry and Veterinary Medicine),23:189-191(周东升,周锦,郑池乐,龙德彪,周云姣,唐红艳,刘畅,张燕芝,王著希,邓小侠,2014,睡眠剥夺对雄性小鼠体重及性行为的影响,黑龙江畜牧兽医,23:189-191)
Gao Y.,Huang Z.F.,Yu H.B.,Huang X.F.,Zhou Y.Y.,and Ma C.,2015,The effect of nano ceria particles pretreatment on oxidative damage of rat myocardial cells,Zhonghua Shiyan Waike Zazhi(Chinese Journal of Experimental Surgery),32(5):1093-1096(高勇,法宪恩,黄真锋,余海彬,周玉阳,马超,2015,纳米二氧化铈颗粒预处理对大鼠心肌细胞氧化损伤的影响,中华实验外科杂志,32(5):1093-1096)
Jiang C.,Zhou R.,Xia C.C.,Feng J.,Li F.G.,Huang Z.Y.,and Cui T.,2013,72 h sleep deprivation induced reproductive toxicity in male mice and its mechanism,Xiandai Yufang Yixue(Modern Preventive Medicine),40(7):1325-1326,1329(蒋超,周冉,夏聪聪,冯健,李丰功,黄振遥,崔涛,2013,72 h睡眠剥夺对雄性小鼠的生殖毒性效应及机制,现代预防医学,40(7):1325-1326,1329)
Kheradmand A.,Alirezaei M.,Asadian P.,Alavi E.R.,and Joorabi S.,2009,Antioxidant enzyme activity and MDA level in the rat testis following chronic administration of ghrelin,Andrologia,41(6):335-340
Liu C.L.,Xu X.,Ha L.X.,Pei L.G.,Jing W.H.,and Wu X.,2015,To study the effect of catalase on oxidative damage in testis of mice,Ningxia Yixue Zazhi(Ningxia Medical Journal),37(9):783-784(刘春莲,徐仙,哈灵侠,裴利国,景万红,吴昕,2015,过氧化氢酶对小鼠睾丸氧化损伤的作用研究,宁夏医学杂志,37(9):783-784)
Liu M.M.,Liu L.,Chen L.,Yin X.J.,Liu H.,Zhang Y.H.,Li P.L.,Wang S.,Li X.X.,and Yu C.H.,2017,Sleep deprivation and late bedtime impair sperm health through increasing antisperm antibody production:a prospective study of 981 healthy men,Med.Sci.Monit.,23:1842-1848
Lv G.M.,Wang Y.J.,Liu R.,Sun L.D.,Huang Y.H.,and Yan C.H.,2013,Biological application of nano cerium oxide,Zhongguo Kexue(Chinese Science:Chemistry),43(10):1309-1321(吕广明,王艳杰,刘瑞,孙聆东,黄云辉,严纯华,2013,纳米氧化铈的抗氧化生物应用,中国科学:化学,43(10):1309-1321)
Rzigalinski B.A.,Carfagna C.S.,and Ehrich M.,2017,Cerium oxide nanoparticles in neuroprotection and considerations for efficacy and safety,Wiley Interdiscip.Rev.Nanomed.Nanobiotechnol.,9(4):10.1002
Shen Y.N.,Guo J.L.,and Liu A.Q.,2013,Cerium oxide and life,Xitu(Rare Earth),34(1):77-80(沈岳年,郭金玲,刘阿秋,2013,纳米氧化铈和生命,稀土,34(1):77-80)
Sinha N.,Narayan R.,and Saxena D.K.,1997,Effect of endosulfan on the testis of growing rats,Bull Environ Contam Toxicol,58(1):79-86
Song B.,and Francis C.,2008,The relationship between androgen and spermatogenesis,Linchuang Miniao Waike Zazhi(Journal of Clinical Department of Urology),23(7):553-555(宋博,蔡志明,2008,雄激素与精子发生的关系,临床泌尿外科杂志,23(7):553-555)
Tobaldini E.,Costantino G.,Solbiati M.,Cogliati C.,Kara T.,Nobili L.,and Montano N.,2016,Sleep,sleep deprivation,autonomic nervous system and cardiovascular diseases,Neurosci.and Biobehav.Rev.,74(Pt B):321-329
Touitou Y.,Reinberg A.,and Touitou D.,2017,Association between light at night,melatonin secretion,sleep deprivation,and the internal clock:health impacts and mechanisms of circadian disruption,Life Sci.,173:94-106
Wu J.L.,Wu S.C.,Yang J.G.,Huang C.C.,Chen K.B.,Fang K.H.,and Tsai H.D.,2011,Effects of sleep deprivation on serum testosterone concentrations in the rat,Neurosci.Lett.,494(2):124
Wu T.,Zhang Z.Q.,Ji L.,Yang T.W.,Zhang B.W.,and Wu G.,2017,Effect of nano cerium oxide on oxidative damage of alveolar macrophages induced by hydrogen peroxide,Huanjing Yu Jiankang Zazhi(Journal of Environment and Health),34(1):9-12(吴涛,张志强,纪乐,杨通旺,张博闻,吴刚,2017,纳米氧化铈对过氧化氢所致大鼠肺泡巨噬细胞氧化损伤的影响,环境与健康杂志,34(1):9-12)
Xie J.R.,Cheng L.L.,Zhu Z.Y.,and Ding Z.Z.,2016,Progress in the study of sleep disorder on reproductive effects,Guoji Shengzhi Jiankang,Jihua Shengyu Zazhi(International Journal of Reproductive Health,Family Planning),35(1):78-82(谢金容,程丽霖,朱真仪,丁之德,2016,睡眠障碍对生殖系统影响的研究进展,国际生殖健康,计划生育杂志,35(1):78-82)
Xu F.,Yang N.,Liang Y.H.,Zheng M.W.,Wang Z.H.,He Q.J.,Shen Y.W.,Li H.Z.,Wang X.Q.,and Lin L.X.,2017,Deep slow wave sleep deprivation effects on oxidative stress in rat testis,Zhonghua Nankexue Zazhi(The Journal of Science),23(8):687-691(徐飞,杨娜,梁钰涵,何其瑾,郑美雯,汪子涵,沈奕薇,李海征,王雪倩,林来祥,2017,深慢波睡眠剥夺对大鼠睾丸组织氧化应激反应的影响,中华男科学杂志,23(8):687-691)
Xu L.C.,Wang S.Y.,and Wang X.R.,2000,Enzymatic study of cadmium toxicity to testis in rats,Zhongguo Gonggong Weisheng(Public Health of China),16(2):119-120(徐莉春,王沭沂,王心如,2000,镉对大鼠睾丸毒作用的酶学研究,中国公共卫生,16(2):119-120)
Yu D.L.,and Wang C.L.,2016,Research progress of catalase,Zhongguo Zuzhi Huaxue Yu Xibao Huaxue Zazhi(Chinese Journal of Histochemistry and Cytochemistry),25(2):189-194(于德玲,王昌留,2016,过氧化氢酶的研究进展,中国组织化学与细胞化学杂志,25(2):189-194)
Zamanian Z.,Nikeghbal K.,and Khajehnasiri F.,2016,Influence of sleep on quality of life among hospital nurses,Electron.Physician,8(1):1811-1816
Zhao Y.F.,Ren F.Q.,Hu K.N.,and Chang D.G.,2015,Sleep deprivation may investigate the mechanism of abnormal spermatozoa,Heilongjiang Yixue(Heilongjiang Medical),10:1165-1166(赵一帆,任飞强,胡开妮,常德贵,2015,睡眠剥夺导致精子质量异常的可能机制探讨,黑龙江医学,10:1165-1166)
Zhou D.S.,Zhou J.,Zheng C.,Long D.B.,Zhou Y.J.,Tang H.Y.,Liu C.,Zhang Y.Z.,Wang X.,and Deng X.X.,2014,The effects of sleep deprivation on body weight and sexual behavior of male mice,Heilongjiang Xumu Shouyi(Heilongjiang Animal Husbandry and Veterinary Medicine),23:189-191(周东升,周锦,郑池乐,龙德彪,周云姣,唐红艳,刘畅,张燕芝,王著希,邓小侠,2014,睡眠剥夺对雄性小鼠体重及性行为的影响,黑龙江畜牧兽医,23:189-191)