摘要
为了探索红花耐旱机制及为选育耐旱品种提供理论依据,笔者研究了干旱胁迫对红花种质萌发及生长状况的影响。采用聚乙二醇(PEG-6000)模拟干旱胁迫处理,对干旱胁迫下10个红花种质萌发率及幼苗生长指标进行测量,并对各测量指标进行模糊隶属函数分析。结果表明,低浓度的PEG对红花种子萌发有一定程度的促进作用;当浓度为15%时,对各指标的抑制作用明显,因此选用15%的PEG浓度鉴定红花种质的抗旱性。在干旱胁迫下红花发芽势、发芽率、下胚轴重、子叶重等降低,胚芽生长受到抑制,干旱对不同种质的根长和根重的影响不一致。通过种子萌发抗旱指数及隶属函数值对红花种质萌发期抗旱性进行综合评价,其中BXY1465为萌发期抗旱性强的材料,BXY512、BXY426、BXY1466以及BXY1859属于萌发期抗旱性中等的材料,BXY1805、BXY2527、BXY2110、BXY750以及WH0086属于萌发期抗性弱的材料。
To explore the drought tolerance mechanism of safflower and provide a basis for breeding drought tolerant varieties, we studied the effect of drought stress on the germination and growth of safflower germplasm.We measured the germination rate and seedling growth index of 10 safflower germplasm under drought stress which simulated by PEG-6000 and analyzed each measurement index by fuzzy subordinate function. The results showed that: low concentration(5%) of PEG had a positive effect on germination of safflower seeds,while 15% of PEG had a significant inhibitory effect on the germination. Therefor, 15% PEG concentration was used to identify the drought resistance of safflower germplasm. Under drought stress, the seed germination energy, the seed germination rate, hypocotyl weight and cotyledon weight of the 10 safflower germplasm all decreased, and the growth of embryos was inhibited; the effects of drought on the length and weight of root of different germplasm were inconsistent. Grain drought resistant index(GDRI) and subordinate function value are used to evaluate the comprehensive drought resistance at germination stage of 10 safflower germplasms,BXY1465 is the material with high drought resistance at germination stage, BXY512, BXY426, BXY1466 and BXY1859 are the materials with moderate drought resistance at germination stage, BXY1805, BXY2527,BXY2110, BXY750 and WH0086 are the materials with low drought resistance at germination stage.
引文
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