活血潜阳方对高血压大鼠内皮祖细胞的影响
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摘要
目的:观察活血潜阳方对高血压大鼠内皮祖细胞的影响。方法:正常血压WKY大鼠10只,自发性高血压大鼠(SHR)30只(随机分为模型组、活血潜阳方组、代文组),WKY组、模型组以生理盐水2 ml/d灌胃,活血潜阳方组予活血潜阳方(0.96 g·kg-1·d-1)、代文组予代文溶液(20 mg·kg-1·d-1)灌胃,共干预8周。在实验前及灌胃4、8周时用尾动脉容积法测定血压,HE染色观察胸主动脉内皮细胞层连续性,并测定循环内皮祖细胞数量及其功能。结果:活血潜阳方组、代文组血压与模型组比较差异有统计学意义(P<0.01),代文组降压效果优于活血潜阳方组(P<0.01)。模型组大鼠胸主动脉内皮层受损最为显著,而活血潜阳方组和代文组大鼠相同部位血管内皮均获一定程度修复,且其循环内皮祖细胞数量及增殖、黏附和迁移能力显著高于模型组(P<0.01,P<0.05)。结论:SHR大鼠胸主动脉内皮细胞完整性严重破坏,循环内皮祖细胞数量减少、功能下降;活血潜阳方可提高循环内皮祖细胞数量和功能。
Objective: To observe the effect of"Huoxue Qianyang Formula"on endothelial progenitor cells in rats with high blood pressure. Methods: Ten rats( WKY) with normal arterial pressure and 30 spontaneously hypertensive rats were randomly divided into model group,"Huoxue Qianyang Formula"group and Diovan group. Rats in the WKY group and model group were gavaged with 2 ml normal saline per day; rats in the"Huoxue Qianyang Formula"group were treated with 0. 96 g·kg- 1·d- 1"Huoxue Qianyang Formula"; Diovan group was treated with 20 mg·kg- 1·d- 1Diovan solution,gavaged for 8 weeks. Blood pressure was detected by tail artery the capacity measure before experiment,4 weeks and 8 weeks gavaged. The continuity of continuity in thoracic aorta was observed by HE-staining. The quantity and function of endothelial progenitor cells were detected. Results: There were significant differences in the blood pressure between "Huoxue Qianyang Formula " group,Diovan group and the model group( P < 0. 01),the efficacy in lower blood pressure of the Diovan group was superior to the"Huoxue Qianyang Formula"group( P < 0. 01). The damage of thoracic aorta endodermis in the model group was significant,and the blood vessel endothelium at the same area was repaired in the"Huoxue Qianyang Formula"group and Diovan group,and the quantity,proliferation,adhesiveness and transfer ability of the endothelial progenitor cells were higher than those of the model group( P < 0. 01,P < 0. 05). Conclusion: The aortic endothelial cells integrity in thoracic aorta of SHR rats were damaged,the quantity of circulating endothelial progenitor cells were reduced and its function was declined; "Huoxue Qianyang Formula"can improve the quantity and function of circulating endothelial progenitor cells.
Objective: To observe the effect of"Huoxue Qianyang Formula"on endothelial progenitor cells in rats with high blood pressure. Methods: Ten rats( WKY) with normal arterial pressure and 30 spontaneously hypertensive rats were randomly divided into model group,"Huoxue Qianyang Formula"group and Diovan group. Rats in the WKY group and model group were gavaged with 2 ml normal saline per day; rats in the"Huoxue Qianyang Formula"group were treated with 0. 96 g·kg- 1·d- 1"Huoxue Qianyang Formula"; Diovan group was treated with 20 mg·kg- 1·d- 1Diovan solution,gavaged for 8 weeks. Blood pressure was detected by tail artery the capacity measure before experiment,4 weeks and 8 weeks gavaged. The continuity of continuity in thoracic aorta was observed by HE-staining. The quantity and function of endothelial progenitor cells were detected. Results: There were significant differences in the blood pressure between "Huoxue Qianyang Formula " group,Diovan group and the model group( P < 0. 01),the efficacy in lower blood pressure of the Diovan group was superior to the"Huoxue Qianyang Formula"group( P < 0. 01). The damage of thoracic aorta endodermis in the model group was significant,and the blood vessel endothelium at the same area was repaired in the"Huoxue Qianyang Formula"group and Diovan group,and the quantity,proliferation,adhesiveness and transfer ability of the endothelial progenitor cells were higher than those of the model group( P < 0. 01,P < 0. 05). Conclusion: The aortic endothelial cells integrity in thoracic aorta of SHR rats were damaged,the quantity of circulating endothelial progenitor cells were reduced and its function was declined; "Huoxue Qianyang Formula"can improve the quantity and function of circulating endothelial progenitor cells.
引文
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[8]符德玉,罗海明,黄蔚,等.活血潜阳方治疗血瘀、阳亢证高血压病患者左心室肥厚的临床研究[J].上海中医药杂志,2003,37(12):6-9.
[9]肖梅芳,赵国定,周端,等.活血潜阳方降压作用及其对高血压病血栓前状态影响的临床研究[J].上海中医药杂志,2013,47(12):46-49.
[10]徐邦杰,符德玉,周训杰,等.活血潜阳方对血瘀阳亢痰浊型SHR大鼠胰岛素抵抗及胰岛素信号通路的影响[J].上海中医药大学学报,2014,28(3):57-62.
[11]周训杰,谭圆圆,符德玉,等.活血潜阳方对高脂饮食加附子灌胃SHR血清AngⅡ含量、IR及脂代谢的影响[J].中西医结合心脑血管病杂志,2012,10(5):577-579.
[2]Imanishi T,Moriwaki C,Hano T,et al.Endothelial progenitor cell senescence is accelerated in both experimental hypertensive rats and patients with essential hypertension[J].J Hypertens,2005,23(10):183l-1837.
[3]符德玉,周端,顾仁樾.活血潜镇胶囊对高血压病(血瘀阳亢证)血管内皮细胞功能影响[J].上海中医药大学学报,2000,14(2):18-20.
[4]Asahara T,Murohara T,Sullivan A,et al.Isolation of putative progenitor endothelial cells for angiogenesis[J].Science,1997,275(5302):964-967.
[5]Fadini GP,Coracina A,Baesso I,et al.Peripheral blood CD34+KDR+endothelial progenitor cells are determinants of subclinical atherosclerosis in a middle-aged general population[J].Stroke,2006,37(9):2277-2282.
[6]Liu P,Zhou B,Gu D,et al.Endothelial progenitor cell therapy in atherosclerosis:a double-edged sword?[J].Ageing Res Rev,2009,8(2):83-93.
[7]胡剑平,谈世进.高血压与内皮祖细胞[J].心血管病学进展,2008,29(1):133-135.
[8]符德玉,罗海明,黄蔚,等.活血潜阳方治疗血瘀、阳亢证高血压病患者左心室肥厚的临床研究[J].上海中医药杂志,2003,37(12):6-9.
[9]肖梅芳,赵国定,周端,等.活血潜阳方降压作用及其对高血压病血栓前状态影响的临床研究[J].上海中医药杂志,2013,47(12):46-49.
[10]徐邦杰,符德玉,周训杰,等.活血潜阳方对血瘀阳亢痰浊型SHR大鼠胰岛素抵抗及胰岛素信号通路的影响[J].上海中医药大学学报,2014,28(3):57-62.
[11]周训杰,谭圆圆,符德玉,等.活血潜阳方对高脂饮食加附子灌胃SHR血清AngⅡ含量、IR及脂代谢的影响[J].中西医结合心脑血管病杂志,2012,10(5):577-579.