葱白提取物对非酒精性脂肪肝模型大鼠ATPase6、ATPase8表达的影响
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摘要
[目的]观察葱白提取物对非酒精性脂肪肝(NAFLD)模型大鼠ATP合酶Fo亚基6(ATPase6)、ATP合酶Fo亚基8(ATPase8)表达的影响,并探讨其作用机制。[方法]将50只雄性SD大鼠随机分为正常组、模型组、葱白低剂量组、葱白中剂量组、葱白高剂量组,每组10只。采用高脂饮食喂养复制NAFLD模型大鼠,造模10周后,给予葱白提取物连续干预4周后,测定血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)和甘油三酯(TG)、胆固醇(TC)的含量;苏木精-伊红(HE)染色法观察肝脏组织结构变化;Real time PCR检测大鼠肝脏组织中ATPase6、ATPase8 mRNA的表达。[结果]与正常组比较,模型组出现明显肝脂肪变性,血清ALT、AST、TC、TG水平明显升高(P<0.05),ATPase6、ATPase8 mRNA的表达明显降低(P<0.05);相较于模型组,葱白低、中、高剂量组肝脂肪变性均不同程度减轻;血清ALT、AST、TC、TG水平均不同程度降低(P<0.05),ATPase6、ATPase8 m RNA表达不同程度升高(P<0.05)。与葱白低剂量组比较,葱白中、高剂量组肝脂肪变性减轻明显,ALT、AST、TC、TG水平明显降低(P<0.05),ATPase6、ATPase8 mRNA水平明显升高(P<0.05),中、高剂量葱白组之间差异无统计学意义。[结论]葱白提取物能够改善肝脂肪变性,可能与提高ATPase6、ATPase8的表达,增加肝线粒体ATP合成有关。
[Objective] To observing the expression of ATPase6 and ATPase8 and explore the probable mechanism of fistular onion stalk extract in treating nonalcoholic fatty liver disease model in rats.[Methods] The 50 male SD rats were randomly divided into normal group,model group,low-dose fistular onion stalk group,medium dose fistular onion stalk group,high-dose fistular onion stalk group,and10 in each group.The rats were fed with a high-fat diet to replicate the NAFLD model.After 10 weeks of modeling,the extract of the onion was given for 4 weeks.Serum levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST) and triglyceride(TC) and cholesterol(TG) were measured.The changes of liver tissue structure wereexpression of ATPase6 and ATPase8 mRNA in liver tissues of rats.[Results] Compared with the normal group,the model group showed obvious hepatic steatosis.The level of serum ALT,AST,TC and TG were higher than normal group(P <0.05).The expression of ATPase6 and ATPase8 mRNA was significantly decreased(P <0.05).Compared with the model group,the lipid degeneration in the liver was alleviated,the level of serum ALT,AST,TC and TG were all decreased,the expression of ATPase6 and ATPase8 mRNA were significantly increased in low-dose fistular onion stalk group,medium dose fistular onion stalk group and high-dose fistular onion stalk group(P<0.05).[Conclusion] Fistular onion stalk extract can improve hepatic steatosis,which may be associated with improved expression of ATPase6 and ATPase8,and increased mitochondrial ATP synthesis.
[Objective] To observing the expression of ATPase6 and ATPase8 and explore the probable mechanism of fistular onion stalk extract in treating nonalcoholic fatty liver disease model in rats.[Methods] The 50 male SD rats were randomly divided into normal group,model group,low-dose fistular onion stalk group,medium dose fistular onion stalk group,high-dose fistular onion stalk group,and10 in each group.The rats were fed with a high-fat diet to replicate the NAFLD model.After 10 weeks of modeling,the extract of the onion was given for 4 weeks.Serum levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST) and triglyceride(TC) and cholesterol(TG) were measured.The changes of liver tissue structure wereexpression of ATPase6 and ATPase8 mRNA in liver tissues of rats.[Results] Compared with the normal group,the model group showed obvious hepatic steatosis.The level of serum ALT,AST,TC and TG were higher than normal group(P <0.05).The expression of ATPase6 and ATPase8 mRNA was significantly decreased(P <0.05).Compared with the model group,the lipid degeneration in the liver was alleviated,the level of serum ALT,AST,TC and TG were all decreased,the expression of ATPase6 and ATPase8 mRNA were significantly increased in low-dose fistular onion stalk group,medium dose fistular onion stalk group and high-dose fistular onion stalk group(P<0.05).[Conclusion] Fistular onion stalk extract can improve hepatic steatosis,which may be associated with improved expression of ATPase6 and ATPase8,and increased mitochondrial ATP synthesis.
引文
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[4]丁晓东,范建高,徐正婕,等.高脂饮食性脂肪性肝炎大鼠肝脏线粒体损伤和ATP储备改变[J].世界华人消化杂志, 2005, 13(13):1524-1528.
[5]张荣,李晓波,徐大志,等.黄皮叶对非酒精性脂肪肝大鼠肝组织ATP和ADP含量的影响[J].中药药理与临床, 2012(2):95-98.
[6]时昭红,张介眉,王湘宁,等.葱白提取物对非酒精性脂肪肝大鼠的治疗作用[C]//世界中医药学会联合会消化病专业委员会首届消化病国际学术大会,2010:2863-2868.
[7]许思思,时昭红,郭洁.葱白提取物调节非酒精性脂肪肝脂质水平的机制研究[J].医学信息,2016,29(36):44-46.
[8]时昭红,张介眉,郑娅琼,等.鲜葱白提取物对实验性非酒精性脂肪肝的药效作用[J].现代消化及介入诊疗,2009,14(3):137-140.
[9]于淼,季宇彬.中药活性成分对非酒精性脂肪肝的作用及机制[J].哈尔滨商业大学学报(自然科学版), 2017, 33(4):403-405.
[10]范建高,曾民德.脂肪性肝病[M].第2版.北京:人民卫生出版社,2013:562.
[11] Day CP. Pathogenesis of steatohepatitis[J]. Best Practice&Research Clinical Gastroenterology,2002,16(5):663-678.
[12] Choi YS, Kim S, Kyu LH, et al. In vitro methylation of nuclear respiratory factor-1 binding site suppresses the promoter activity of mitochondrial transcription factor A[J]. Biochemical&Biophysical Research Communications,2004,314(1):118-122.
[13] Wu Z, Boss O. Targeting PGC-1αto control energy homeostasis[J].Expert Opinion on Therapeutic Targets,2007,11(10):1329-1338.
[14] Koch-Koerfges A, Kabus A, Ochrombel I, et al. Physiology and global gene expression of a Corynebacterium glutamicumΔF(1)F(O)-ATP synthase mutant devoid of oxidative phosphorylation[J].Biochimica Et Biophysica Acta, 2012, 1817(2):370-380.
[15]郝建军,张介眉,时昭红,等.张介眉教授运用“通阳法”异病同治的临床和实验研究[C],2012.
[16]户菲菲,张介眉,郝建军.痰瘀交阻与通阳[J].天津中医药,2010,27(4):303-304.
[17]户菲菲,张介眉,朱旭,等.通阳药葱白提取物对肝窦毛细血管化大鼠肝组织Ⅳ型胶原的影响[J].中西医结合肝病杂志,2010,20(1):32-34.
[18]户菲菲,张介眉,朱旭,等.通阳药葱白提取物对肝窦毛细血管化大鼠肝组织MMP-2,TIMP-2蛋白及mRNA表达影响的研究[J].成都中医药大学学报,2010,33(4):55-58.
[2] Forbes S, Taylorrobinson SD, Patel N, et al. Increased prevalence of non-alcoholic fatty liver disease in European women with a history of gestational diabetes[J]. Diabetologia,2011,54(3):641-647.
[3]樊丽琳,陈东风.肝脏线粒体呼吸功能损伤在大鼠非酒精性脂肪性肝病中的作用[J].重庆医学, 2007, 36(15):1503-1505.
[4]丁晓东,范建高,徐正婕,等.高脂饮食性脂肪性肝炎大鼠肝脏线粒体损伤和ATP储备改变[J].世界华人消化杂志, 2005, 13(13):1524-1528.
[5]张荣,李晓波,徐大志,等.黄皮叶对非酒精性脂肪肝大鼠肝组织ATP和ADP含量的影响[J].中药药理与临床, 2012(2):95-98.
[6]时昭红,张介眉,王湘宁,等.葱白提取物对非酒精性脂肪肝大鼠的治疗作用[C]//世界中医药学会联合会消化病专业委员会首届消化病国际学术大会,2010:2863-2868.
[7]许思思,时昭红,郭洁.葱白提取物调节非酒精性脂肪肝脂质水平的机制研究[J].医学信息,2016,29(36):44-46.
[8]时昭红,张介眉,郑娅琼,等.鲜葱白提取物对实验性非酒精性脂肪肝的药效作用[J].现代消化及介入诊疗,2009,14(3):137-140.
[9]于淼,季宇彬.中药活性成分对非酒精性脂肪肝的作用及机制[J].哈尔滨商业大学学报(自然科学版), 2017, 33(4):403-405.
[10]范建高,曾民德.脂肪性肝病[M].第2版.北京:人民卫生出版社,2013:562.
[11] Day CP. Pathogenesis of steatohepatitis[J]. Best Practice&Research Clinical Gastroenterology,2002,16(5):663-678.
[12] Choi YS, Kim S, Kyu LH, et al. In vitro methylation of nuclear respiratory factor-1 binding site suppresses the promoter activity of mitochondrial transcription factor A[J]. Biochemical&Biophysical Research Communications,2004,314(1):118-122.
[13] Wu Z, Boss O. Targeting PGC-1αto control energy homeostasis[J].Expert Opinion on Therapeutic Targets,2007,11(10):1329-1338.
[14] Koch-Koerfges A, Kabus A, Ochrombel I, et al. Physiology and global gene expression of a Corynebacterium glutamicumΔF(1)F(O)-ATP synthase mutant devoid of oxidative phosphorylation[J].Biochimica Et Biophysica Acta, 2012, 1817(2):370-380.
[15]郝建军,张介眉,时昭红,等.张介眉教授运用“通阳法”异病同治的临床和实验研究[C],2012.
[16]户菲菲,张介眉,郝建军.痰瘀交阻与通阳[J].天津中医药,2010,27(4):303-304.
[17]户菲菲,张介眉,朱旭,等.通阳药葱白提取物对肝窦毛细血管化大鼠肝组织Ⅳ型胶原的影响[J].中西医结合肝病杂志,2010,20(1):32-34.
[18]户菲菲,张介眉,朱旭,等.通阳药葱白提取物对肝窦毛细血管化大鼠肝组织MMP-2,TIMP-2蛋白及mRNA表达影响的研究[J].成都中医药大学学报,2010,33(4):55-58.