土壤生物消毒对番茄青枯病的防控、土壤理化特性和微生物群落的影响
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摘要
为探索土传病害番茄青枯病防治的有效途径,通过盆栽实验研究了土壤添加米糠及灌水厌氧处理方法对青枯病的控制效果和土壤理化特性的影响。结果表明:与对照(CK)相比,添加2.0%米糠(B)、添加2.0%米糠+淹水(DB)和添加2.0%米糠+密闭厌氧(ADB)对青枯病的防效分别为82.5%、90%和100%,且ADB处理对番茄株高、茎粗和鲜重分别提高176%、111%和755%;3种处理对土壤理化特性也产生了影响,其中DB和ADB处理显著提高土壤温度、pH和电导率(EC),降低氧化还原电位(Eh),而且ADB还明显提高土壤中琥珀酸、反丁烯二酸和柠檬酸的含量;此外,DB和ADB处理减少土壤细菌、放线菌和真菌数量,但显著增加细菌/真菌、细菌/放线菌的比例,同时还显著降低土壤和植株中青枯菌数量。研究表明,DB和ADB两种厌氧处理均能显著改善土壤理化特性和调控微生物群落结构,抑制青枯菌和减少番茄青枯病的发生。
Rice bran application and anaerobic treatment with irrigation were conducted to explore the effective ways of prevention and control of bacterial wilt. The results showed that the effects of rice bran(B),rice bran + irrigation(DB) and rice bran + anaerobic disinfestation(ADB) on bacterial wilt suppression were 82.5%,90% and 100%,respectively. ADB treatment significantly increased plant height,stem diameter and fresh weight of tomato by 176%,111%and 755%,respectively. In comparison with the control,DB and ADB treatments significantly increased soil temperature,pH and conductivity(EC),but reduced redox potential(Eh); ADB treatment significantly increased the contents of succinic acid,fumaric acid and citric acid in soil.In addition,DB and ADB treatments reduced the amounts of soil bacteria,actinomycetes and fungi,but increased the ratios of bacteria/fungi and bacteria/actinomycetes,and significantly reduced the amount of Ralstonia solanacearum in soil and plant stems. This study indicated that both DB and ADB treatments could significantly improve soil physicochemical properties and regulate soil microbial community structure,thus inhibiting R. solanacearum development and reducing the occurrence of tomato bacterial wilt.
Rice bran application and anaerobic treatment with irrigation were conducted to explore the effective ways of prevention and control of bacterial wilt. The results showed that the effects of rice bran(B),rice bran + irrigation(DB) and rice bran + anaerobic disinfestation(ADB) on bacterial wilt suppression were 82.5%,90% and 100%,respectively. ADB treatment significantly increased plant height,stem diameter and fresh weight of tomato by 176%,111%and 755%,respectively. In comparison with the control,DB and ADB treatments significantly increased soil temperature,pH and conductivity(EC),but reduced redox potential(Eh); ADB treatment significantly increased the contents of succinic acid,fumaric acid and citric acid in soil.In addition,DB and ADB treatments reduced the amounts of soil bacteria,actinomycetes and fungi,but increased the ratios of bacteria/fungi and bacteria/actinomycetes,and significantly reduced the amount of Ralstonia solanacearum in soil and plant stems. This study indicated that both DB and ADB treatments could significantly improve soil physicochemical properties and regulate soil microbial community structure,thus inhibiting R. solanacearum development and reducing the occurrence of tomato bacterial wilt.
引文
陈玉婷,林威鹏,范雪滢,等.2015.硅介导番茄青枯病抗性的土壤定量蛋白质组学研究.土壤学报,51(1):162-173.
戴志刚.2009.秸秆养分释放规律及秸秆还田对作物产量和土壤肥力的影响(硕士学位论文).武汉:华中农业大学.
戴志刚,鲁剑巍,鲁明星,等.2010.油菜秸秆用量对淹水培养土壤表层溶液理化性质的影响.水土保持学报,24(1):197-201.
顾志光,马艳,安霞,等.2014.麦秸淹水处理对连作土壤性状和辣椒疫病田间防控效果的影响.农业环境科学学报,33(9):1762-1769.
黄新琦,温腾,孟磊,等.2014.土壤快速强烈还原对于尖孢镰刀菌的抑制作用.生态学报,34(16):4526-4534.
黄俊丽.2003.有机改良剂对棉花黄萎病的防治效果及其防病机制研究(硕士学位论文).郑州:河南农业大学.
何海斌,王海斌,陈祥旭,等.2007.化感水稻苗期不同器官水浸提液及根系分泌物对稗草的化感作用,中国农业生态学报,15(2):14-17.
胡可,李华兴,卢维盛,等.2010.生物有机肥对土壤微生物活性的影响.中国生态农业学报,18(2):303-306.
金彩霞,朱雯斐,李明亮,等.2013.作物根际土壤有机酸含量动态变化研究.干旱区资源与环境,27(11):86-91.
刘星,张书乐,刘国锋,等.2015.土壤生物消毒对甘肃省中部沿黄灌区马铃薯连作障碍的防控效果.应用生态学报,26(4):1205-1214.
刘肖肖,董元华,李建刚.2013.不同土壤改良剂对番茄青枯病的防治效果.农业环境科学学报,32(7):1368-1374.
乔俊卿,陈志谊,刘邮洲,等.2013.茄科作物青枯病研究进展.植物病理学报,(1):1-10.
单玉华,蔡祖聪,韩勇,等.2006.淹水土壤有机酸积累与秸秆碳氮比及氮供应的关系.土壤学报,43(6):941-947.
单玉华,李昌贵,陈晨,等.2008.施用秸秆对淹水土壤镉、铜溶出的影响.生态学杂志,27(8):1362-1366.
汪国平,袁四清,熊正葵,等.2003.广东省番茄青枯病相关研究概况.广东农业科学,(3):32-34.
王光飞,马艳,常志州,等.2013.淹水改良土壤性状及对辣椒疫病的防效研究.水土保持学报,27(2):209-214.
朱同彬,孟天竹,张金波,等.2013.强还原方法对退化设施蔬菜地土壤的修复.应用生态学报,24(9):2619-2624.
朱同彬,孙盼盼,党琦,等.2014.淹水添加有机物料改良退化设施蔬菜地土壤.土壤学报,52(2):335-341.
周开胜.2015.厌氧还原土壤灭菌法抑制西瓜专化型尖孢镰刀菌.江苏农业学报,31(5):1006-1011.
Butler DM,Kokalis-Burelle N,Muramoto J,et al.2012.Impac of anaerobic soil disinfestation combined with soil solarization on plant-parasitic nematodes and introduced inoculum of soilborne plant pathogens in raised-bed vegetable production.Crop Protection,39:33-40.
Blok WJ,Lamers JG,Termorshuizen AJ,et al.2000.Control o soilborne plant pathogens by incorporating fresh organic amendments followed by tarping.Phytopathology,90:253-259.
Diogo RVC,Wydra K.2007.Silicon-induced basal resistance in tomato against Ralstonia solanacearum is related to modification of pectic cell wall polysaccharide structure.Physiological and Molecular Plant Pathology,70:120-129.
Goud JC,Termorshuizen AJ,Blok WJ,et al.2004.Long-term effect of biological soil disinfestation on Verticillium wilt Plant Disease,88:688-694.
Huang XQ,Wen T,Zhang JB,et al.2015.Toxic organic acids produced in biological soil disinfestation mainly caused the suppression of Fusarium oxysporum f.sp.cubense.Bio Control,60:113-124.
Horita M,Kitamoto HK.2015.Biological soil disinfestation using bioethanol fermentation products:Role of residual organic substances.Journal of General Plant Pathology,81:304-314.
Hewavitharana SS,Ruddell D,Mazzola M.2014.Carbon source-dependent antifungal and nematicidal volatiles derived during anaerobic soil disinfestation.European Journal of Plant Pathology,140:39-52.
Koike ST,Gordon TR.2015.Management of Fusarium wilt of strawberry.Crop Protection,73:67-72.
Lamers JG,Runia WT,Molendijk L,et al.2010.Perspectives of anaerobic soil disinfestation.Acta Horticulturae,883:277-283.
Li JG,Dong YH.2013.Effect of a rock dust amendment on disease severity of tomato bacterial wilt.Antonie Van Leeuwenhoek,103:11-22.
Liu LL,Kong JJ,Cui HL,et al.2016.Relationships of decomposability and C/N ratio in different types of organic matter with suppression of Fusarium oxysporum and microbial communities during reductive soil disinfestation.Biological Control,101:103-113.
Messiha NAS,Diepeningen AD,Wenneker M,et al.2007.Biological Soil Disinfestation(BSD),a new control method for potato brown rot,caused by Ralstonia solanacearum race 3biovar 2.European Journal of Plant Pathology,117:403-415.
Momma N,Kobara Y,Uematsu S,et al.2013.Development of biological soil disinfestations in Japan.Applied Microbiology and Biotechnology,97:3801-3809.
Momma N.2008.Biological soil disinfestation(BSD)of soilborne pathogens and its possible mechanisms.Japan Agricultural Research Quarterly,42:7-12.
Overbeek LV,Runia W,Kastelein P,et al.2014.Anaerobic disinfestation of tare soils contaminated with Ralstonia solanacearum biovar 2 and Globodera pallida.European Journal of Plant Pathology,138:323-330.
Shrestha U,AugéRM,Butler DM.2016.A meta-analysis of the impact of anaerobic soil disinfestation on pest suppression and yield of horticultural crops.Frontiers in Plant Science,7:1254.
Strauss SL,Kluepfel DA.2015.Anaerobic soil disinfestation:Achemical-independent approach to pre-plant control of plant pathogens.Journal of Integrative Agriculture,14:2309-2318.
Tomihama T,Nishi Y,Mori K,et al.2016.Rice bran amendment suppresses potato common scab by increasing antagonistic bacterial community levels in the rhizosphere.Phytopathology,106:719-728.
Zhu TB,Dang Q,Zhang JB,et al.2014.Reductive soil disinfestation(RSD)alters gross N transformation rates and reduces NO and N2O emissions in degraded vegetable soils.Plant and Soil,382:269-280.
戴志刚.2009.秸秆养分释放规律及秸秆还田对作物产量和土壤肥力的影响(硕士学位论文).武汉:华中农业大学.
戴志刚,鲁剑巍,鲁明星,等.2010.油菜秸秆用量对淹水培养土壤表层溶液理化性质的影响.水土保持学报,24(1):197-201.
顾志光,马艳,安霞,等.2014.麦秸淹水处理对连作土壤性状和辣椒疫病田间防控效果的影响.农业环境科学学报,33(9):1762-1769.
黄新琦,温腾,孟磊,等.2014.土壤快速强烈还原对于尖孢镰刀菌的抑制作用.生态学报,34(16):4526-4534.
黄俊丽.2003.有机改良剂对棉花黄萎病的防治效果及其防病机制研究(硕士学位论文).郑州:河南农业大学.
何海斌,王海斌,陈祥旭,等.2007.化感水稻苗期不同器官水浸提液及根系分泌物对稗草的化感作用,中国农业生态学报,15(2):14-17.
胡可,李华兴,卢维盛,等.2010.生物有机肥对土壤微生物活性的影响.中国生态农业学报,18(2):303-306.
金彩霞,朱雯斐,李明亮,等.2013.作物根际土壤有机酸含量动态变化研究.干旱区资源与环境,27(11):86-91.
刘星,张书乐,刘国锋,等.2015.土壤生物消毒对甘肃省中部沿黄灌区马铃薯连作障碍的防控效果.应用生态学报,26(4):1205-1214.
刘肖肖,董元华,李建刚.2013.不同土壤改良剂对番茄青枯病的防治效果.农业环境科学学报,32(7):1368-1374.
乔俊卿,陈志谊,刘邮洲,等.2013.茄科作物青枯病研究进展.植物病理学报,(1):1-10.
单玉华,蔡祖聪,韩勇,等.2006.淹水土壤有机酸积累与秸秆碳氮比及氮供应的关系.土壤学报,43(6):941-947.
单玉华,李昌贵,陈晨,等.2008.施用秸秆对淹水土壤镉、铜溶出的影响.生态学杂志,27(8):1362-1366.
汪国平,袁四清,熊正葵,等.2003.广东省番茄青枯病相关研究概况.广东农业科学,(3):32-34.
王光飞,马艳,常志州,等.2013.淹水改良土壤性状及对辣椒疫病的防效研究.水土保持学报,27(2):209-214.
朱同彬,孟天竹,张金波,等.2013.强还原方法对退化设施蔬菜地土壤的修复.应用生态学报,24(9):2619-2624.
朱同彬,孙盼盼,党琦,等.2014.淹水添加有机物料改良退化设施蔬菜地土壤.土壤学报,52(2):335-341.
周开胜.2015.厌氧还原土壤灭菌法抑制西瓜专化型尖孢镰刀菌.江苏农业学报,31(5):1006-1011.
Butler DM,Kokalis-Burelle N,Muramoto J,et al.2012.Impac of anaerobic soil disinfestation combined with soil solarization on plant-parasitic nematodes and introduced inoculum of soilborne plant pathogens in raised-bed vegetable production.Crop Protection,39:33-40.
Blok WJ,Lamers JG,Termorshuizen AJ,et al.2000.Control o soilborne plant pathogens by incorporating fresh organic amendments followed by tarping.Phytopathology,90:253-259.
Diogo RVC,Wydra K.2007.Silicon-induced basal resistance in tomato against Ralstonia solanacearum is related to modification of pectic cell wall polysaccharide structure.Physiological and Molecular Plant Pathology,70:120-129.
Goud JC,Termorshuizen AJ,Blok WJ,et al.2004.Long-term effect of biological soil disinfestation on Verticillium wilt Plant Disease,88:688-694.
Huang XQ,Wen T,Zhang JB,et al.2015.Toxic organic acids produced in biological soil disinfestation mainly caused the suppression of Fusarium oxysporum f.sp.cubense.Bio Control,60:113-124.
Horita M,Kitamoto HK.2015.Biological soil disinfestation using bioethanol fermentation products:Role of residual organic substances.Journal of General Plant Pathology,81:304-314.
Hewavitharana SS,Ruddell D,Mazzola M.2014.Carbon source-dependent antifungal and nematicidal volatiles derived during anaerobic soil disinfestation.European Journal of Plant Pathology,140:39-52.
Koike ST,Gordon TR.2015.Management of Fusarium wilt of strawberry.Crop Protection,73:67-72.
Lamers JG,Runia WT,Molendijk L,et al.2010.Perspectives of anaerobic soil disinfestation.Acta Horticulturae,883:277-283.
Li JG,Dong YH.2013.Effect of a rock dust amendment on disease severity of tomato bacterial wilt.Antonie Van Leeuwenhoek,103:11-22.
Liu LL,Kong JJ,Cui HL,et al.2016.Relationships of decomposability and C/N ratio in different types of organic matter with suppression of Fusarium oxysporum and microbial communities during reductive soil disinfestation.Biological Control,101:103-113.
Messiha NAS,Diepeningen AD,Wenneker M,et al.2007.Biological Soil Disinfestation(BSD),a new control method for potato brown rot,caused by Ralstonia solanacearum race 3biovar 2.European Journal of Plant Pathology,117:403-415.
Momma N,Kobara Y,Uematsu S,et al.2013.Development of biological soil disinfestations in Japan.Applied Microbiology and Biotechnology,97:3801-3809.
Momma N.2008.Biological soil disinfestation(BSD)of soilborne pathogens and its possible mechanisms.Japan Agricultural Research Quarterly,42:7-12.
Overbeek LV,Runia W,Kastelein P,et al.2014.Anaerobic disinfestation of tare soils contaminated with Ralstonia solanacearum biovar 2 and Globodera pallida.European Journal of Plant Pathology,138:323-330.
Shrestha U,AugéRM,Butler DM.2016.A meta-analysis of the impact of anaerobic soil disinfestation on pest suppression and yield of horticultural crops.Frontiers in Plant Science,7:1254.
Strauss SL,Kluepfel DA.2015.Anaerobic soil disinfestation:Achemical-independent approach to pre-plant control of plant pathogens.Journal of Integrative Agriculture,14:2309-2318.
Tomihama T,Nishi Y,Mori K,et al.2016.Rice bran amendment suppresses potato common scab by increasing antagonistic bacterial community levels in the rhizosphere.Phytopathology,106:719-728.
Zhu TB,Dang Q,Zhang JB,et al.2014.Reductive soil disinfestation(RSD)alters gross N transformation rates and reduces NO and N2O emissions in degraded vegetable soils.Plant and Soil,382:269-280.