摘要
目的探讨异甘草素(ISL)对人骨肉瘤细胞MG-63增殖及侵袭的影响及可能机制。方法采用不同浓度异甘草素(0,5,10,20μg/ml)作用于MG-63细胞后,四甲基偶氮唑蓝(MTT)比色法观察ISL对MG-63细胞生长的抑制作用。单一浓度的异甘草素(20μg/ml)处理MG-63细胞48h后,Transwell实验检测MG-63细胞侵袭能力变化,Western blot方法检测Bcl-2、Bax及自噬相关蛋白Beclin1、LC3的表达,实时PCR方法检测Bcl-2mRNA与Bax mRNA的表达水平。结果 ISL以浓度依赖性抑制MG-63细胞增殖。经20μg/ml异甘草素处理后,MG-63细胞侵袭能力显著降低,Bcl-2蛋白的表达下调,Bax及自噬相关蛋白Beclin1、LC3蛋白的表达上调,Bcl-2 mRNA的表达水平下调,Bax mRNA的表达水平上调。结论异甘草素在体外能够抑制MG-63细胞增殖与侵袭,与激活线粒体凋亡通路及自噬通路相关。
Objective To investigate the effect of isoliquiritigenin(ISL) on the proliferation and invasion of human osteosarcoma cell line MG-63 and its possible mechanism. Methods The effects of ISL on MG-63 cells proliferation was observed by methyl thiazolyl tetrazolium(MTT) after different concentrations of ISL(0,5,10,20 ug/ml) was treated. 48 later, treatment with a single concentration of isoglycyrrhizin(μg/ml), the invasive ability of MG-63 cells was detected by Transwell assay, the expressions of Bcl-2, Bax, autophagy-related proteins Beclin1 and LC3 were detected by Western blot, and the expressions of Bcl-2 mRNA and Bax mRNA were detected by real-time PCR. Results ISL inhibited the proliferation of MG-63 cells in a concentration dependent manner. After treatment with 20 μg/ml ISL, the invasive ability of MG-63 cells was significantly decreased, and the expression level of Bcl-2 protein and m RNA was downregulated, the expression level of Bax protein and mRNA, and autophagy-related proteins Beclin1 and LC3 were upregulated. Conclusion ISL inhibited the proliferation and invasion of MG-63 cells in vitro, which was related to the activation of mitochondrial apoptosis pathway and autophagy pathway.
引文
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