广东石豆兰多糖的提取工艺及其抗氧化活性
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摘要
为优化广东石豆兰多糖的提取工艺并评价其抗氧化活性。在单因素试验基础上,采用正交设计和方差分析,研究液料比、提取温度、时间及沉淀多糖的乙醇浓度对石豆兰多糖浸提量的影响并优化了工艺条件,确定最优条件为液料比50∶1 (mL/g),提取温度90℃,时间6 h,醇沉浓度为95%,此条件下多糖提取量为79.060 mg/g,RSD为0.132%。抗氧化结果表明广东石豆兰多糖的总还原力为L-抗坏血酸的3.46%;对DPPH、·OH、·O■自由基半清除浓度(EC_(50))分别为2769.58、594.60、586.94μg/mL,其清除能力分别是L-抗坏血酸的0.68%、47.17%和29.00%;对Fe~(2+)的半螯合浓度(EC_(50))为160.83μg/mL,螯合能力是EDTA的2.12%。本研究结果为石豆兰多糖的提取及进一步开发利用提供参考依据。
In this experiment,optimization of extraction process of polysaccharide from Bublophyllum kwangtungense and its antioxidant activity were studied.On the basis of single factors experiments,orthogonal design and variance analysis were ultilized to investigate the effects of liquid-to-solid,extraction temperature and time,ethanol concentration for precipitation on the content of polysaccharide,and optimize the process.The optimal extraction conditions were determined as follows∶liquid-to-solid,50∶1(mL/g);extraction temperature,90 ℃,and extraction time,6 h;ethanol heavy concentration,95%.Under the optimal conditions,the yield of polysaccharide of 79.060 mg/g was gained,and the RSD value was 0.132%.Polysaccharide displays good antioxidant activity with that total reducing power is 0.035 times of L-ascorbic acid,and the EC_(50) values for DPPH,hydroxyl radicals and superoxide anions are 2769.58,594.60,586.94 μg/mL,and the cleanrance ability are 0.68%,47.17% and 29.00% of L-ascorbic acid respectively.The chelating rate to Fe~(2+) with EC_(50) values of 160.83 μg/mL,and the chelating ability is 2.12% of EDTA.These results provided a reference for the extraction,further development and ultilization of the polysaccharide in B.kwangtungense.
In this experiment,optimization of extraction process of polysaccharide from Bublophyllum kwangtungense and its antioxidant activity were studied.On the basis of single factors experiments,orthogonal design and variance analysis were ultilized to investigate the effects of liquid-to-solid,extraction temperature and time,ethanol concentration for precipitation on the content of polysaccharide,and optimize the process.The optimal extraction conditions were determined as follows∶liquid-to-solid,50∶1(mL/g);extraction temperature,90 ℃,and extraction time,6 h;ethanol heavy concentration,95%.Under the optimal conditions,the yield of polysaccharide of 79.060 mg/g was gained,and the RSD value was 0.132%.Polysaccharide displays good antioxidant activity with that total reducing power is 0.035 times of L-ascorbic acid,and the EC_(50) values for DPPH,hydroxyl radicals and superoxide anions are 2769.58,594.60,586.94 μg/mL,and the cleanrance ability are 0.68%,47.17% and 29.00% of L-ascorbic acid respectively.The chelating rate to Fe~(2+) with EC_(50) values of 160.83 μg/mL,and the chelating ability is 2.12% of EDTA.These results provided a reference for the extraction,further development and ultilization of the polysaccharide in B.kwangtungense.
引文
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2 Ma L,Zhai JW,Chen SP,et al.Bulbophyllum pteroglossum Schltr.a newly recorded species of Orchidaceae from Tibet[J].J Fujian Agric For Univ:Nat Sci(福建农林大学学报:自科版),2017,46:402-403.
3 Wang XL,Wang CW,Li JW,et al.Bulbophyllum hirtum(Sm) Lindl ex Wall —a newly recorded species of Orchidaceae in Tibet[J].For Inven Plan(林业调查规划),2018,45(3):80-82.
4 Wu B,Wu DK,Chen JB.In vitro anti-tumor experiment on extraction of differnt parts of Bulbophyllum kwangtungense[J].J Nangjing Univ Tradit Chin Med(南京中医药大学学报),2004,20:114-115.
5 Liu L,Xiao FH.Research progress of polysaccharides from dendrobium medicinal plants[J].Res Pract Chin Med(现代中药研究与实践),2009,23(1):77-80.
6 Cui SW,Luo SQ,Zhou JQ,et al.Research advances on Dendrobium huoshanense polysaccharide[J].Food Res & Dev(食品研究与开发),2018,39:207-213.
7 Shang J.Progress on extraction of polysaccharide from Bletilla Striata and its application in food[J].Mod Food(现代食品),2018,1:124-127.
8 Xiao XH,Lin CX,Wu XL,et al.Research advance on chemical constituents and biological activities of Anoectochilus roxburghii[J].Mod Food Sci Tec(现代食品科技),2018,34:267-275.
9 Yang HH,Weng HM,Guang YG,et al.Study on extraction and purification of polysaccharides from Pholidota chinensis Lindl[J].Food Sci(食品科学),2009,30:130-133.
10 Wang Q,Xiao Y,Zhang RZ,et al.Research progress on extraction,separation,purification and bioactivity of polysaccharides in Gastrodia elata Bl.[J].J Tongren Univ(铜仁学院学报),2017,6:19-24.
11 Liu RL,Ma DX,Yu YF.A study on the relationship between the Buulbophyllum kwangtunense microscopic structure features and its resistibility to dry eco-habitat[J].J Hebei Normal Univ:Nat Sci(河北师范大学学报:自科版),2008,32:817-820.
12 Wu B.Studies on the chemical constituents of the plant Bulbophyllum kwangtungense[D].Nanjing:Nanjing University of Chinese Medicine(南京中医药大学),2004.
13 Chen YG,Xu JJ,Gao F,Chen YP.Advance on the chemical and pharmacological studies on plants of Bulbophyllum genus[J].J Wuhan Bot Res(武汉植物学研究),2005,23:601-602.
14 Zhao HR,Wang YZ,Xu RL,et al.Extraction of polysaccharide and content determination of Yunnan Orchidaceae plants[J].J Dali Univ(大理大学学报),2016,1(4):26-28.
15 Apati P,Szentmihalyi K,Kristo ST,et al.Herbal remedies of solidago-correlation of phytochemical characteristics and antioxidant properties[J].J Phar and Bio Anal,2003,32:1045-1053.
16 Hatano T,Kagawa H,Yasuhar AT,et al.Two new flavonoids and other constituents in licorice root:their relative astringency and scavenging effects[J].Chem and Phar Bull,1988,36:2090-2097.