池塘工程化循环水养殖模式下养殖密度对大口黑鲈生长性能和生理指标的影响
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摘要
为研究池塘工程化循环水养殖模式下养殖密度对大口黑鲈(Micropterus salmoides)生长性能、血清生化指标的影响,设置15 000和25 000尾/槽2个养殖密度,经过120 d的养殖实验。结果显示:养殖密度对大口黑鲈生长性能、血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶、碱性磷酸酶、皮质醇和溶菌酶含量无显著影响;高养殖密度组血清总蛋白、胆固醇、甘油三酯和葡萄糖含量显著低于低养殖密度组。在低、高密度养殖条件下,体重与体长之间呈幂函数关系,生长方程分别为y=0.031 6x~(2.903 8)(R~2=0.980 1)和y=0.032 1x~(2.898 5)(R~2=0.982 4)。结果表明,池塘工程化循环水养殖模式下,高密度养殖而不影响大口黑鲈生长。
An 120-day feeding experiment was performed to asses the effect of stocking densities on growth performance and serum biochemical parameters of largemouth bass(Micropterus salmoides) reared in an in-pond raceway system(IPRS). Fish were randomly allotted to four raceways stocked at two stocking densities(15 000 and 25 000 tails/raceway, respectively). No significant difference was observed in growth, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, superoxide dismutase, cortisol and lysozyme in serum between fish reared at two stocking groups. Fish reared at high stocking density had significant lower total protein, cholesterol, triglyceride and glucose content in serum than those reared at low density. Under the low and high density condition, body weight and body length had power function correlation. The growth equations were y = 0.0316x~(2.9038 )(R~2=0.9801), y=0.0321x~(2.8985 )(R~2=0.9824), respectively. The present results indicated that the largemouth bass could be reared at high stocking density without negatively affecting growth in IPRS under these experimental conditions.
An 120-day feeding experiment was performed to asses the effect of stocking densities on growth performance and serum biochemical parameters of largemouth bass(Micropterus salmoides) reared in an in-pond raceway system(IPRS). Fish were randomly allotted to four raceways stocked at two stocking densities(15 000 and 25 000 tails/raceway, respectively). No significant difference was observed in growth, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, superoxide dismutase, cortisol and lysozyme in serum between fish reared at two stocking groups. Fish reared at high stocking density had significant lower total protein, cholesterol, triglyceride and glucose content in serum than those reared at low density. Under the low and high density condition, body weight and body length had power function correlation. The growth equations were y = 0.0316x~(2.9038 )(R~2=0.9801), y=0.0321x~(2.8985 )(R~2=0.9824), respectively. The present results indicated that the largemouth bass could be reared at high stocking density without negatively affecting growth in IPRS under these experimental conditions.
引文
[1]宋超,孟顺龙,范立民,等.中国淡水池塘养殖面临的环境问题及对策[J].中国农学通报,2012,28(26) :89-92.
[2]范立民,徐跑,吴伟,等.淡水养殖池塘微生态环境调控研究综述[J].生态学杂志,2013,32(11) :3094-3100.
[3]Brown T W,Chappell J A,Boyd C E.A commercial-scale,in-pond raceway system for Ictalurid catfish production [J].Aquacult Eng,2011,44(3) :72-79.
[4]Naderi M,Keyvanshokooh S,Salati A P,et al.Combined or individual effects of dietary vitamin E and selenium nanoparticles on humoral immune status and serum parameters of rainbow trout(Oncorhynchus mykiss) under high stocking density[J].Aquaculture,2017,474 :40-47.
[5]Andrade T,Afonso A,Pérez-Jiménez A,et al.Evaluation of different stocking densities in a Senegalese sole(Solea senegalensis) farm:Implications for growth,humoral immune parameters and oxidative status [J].Aquaculture,2015,438:6-11.
[6] Di Marco P,Priori A,Finoia M G,et al.Physiological responses of European sea bass Dicentrarchus labrax to different stocking densities and acute stress challenge [J].Aquaculture,2008,275 :319-328.
[7]Ellis T,North B,Scott A P.Therelationships between stocking density and welfare in farmed rainbow trout [J].J Fish Biol,2002,61(3) :493-531.
[8] Millán-Cubillo A F,Martos-Sitcha J A,Ruiz-Jarabo I,et al.Low stocking density negatively affects growth,metabolism and stress pathways in juvenile specimens of meagre(Argyrosomus regius,Asso 1801) [J].Aquaculture,2016,451 :87-92.
[9]Pirozzi I,Booth M,Pankhurst P.The effect of stocking density and repeated handling on the growth of juvenile mulloway,Argyrosomus japonicus(Temminck & Schlegel 1843)[J].Aquac Int,2009,17(2):199-205.
[10]Liu B L,Jia R,Han C,et al.Effects of stocking density on antioxidant status,metabolism and immune response in juvenile turbot(Scophthalmus maximus) [J].Comp Biochem Phys C,2016,190 :1-8.
[11]Montero D,Izquierdo M S,Tort L,et al.High stocking density produces crowding stress altering some physiological and biochemical parameters in gilthead seabream,Sparus aurata,juveniles [J].Fish Physiol Biochem,1999,20(1) :53-60.
[12] Anderson D,Saoud I P,Davis D A.The effects of stocking density on survival,growth,condition,and feed efficiency of Bluegill juveniles [J].N Am J Aquac,2002,64(4) :297-300
[13]王炳谦,王芳,谷伟,等.不同养殖密度对褐鳟稚鱼生长性能的影响[J].东北农业大学学报,2014,45(12):18-23.
[14]Telli G S,Ranzani-Paiva M J T,Dias D C,et al.Dietary administration of Bacillus subtilis on hematology and non-specific immunity of Nile tilapia raised at different stocking densities [J].Fish Shellfish Immun,2014,39(2) :305-311.
[15]Garcia F,Romera D M,Gozi K S,et al.Stocking density of Nile tilapia in cages placed in a hydroelectric reservoir [J].Aquaculture,2013,410:51-56.
[16] Lupatsch I,Santos G A,Schrama J W,et al.Effect of stocking density and feeding level on energy expenditure and stress responsiveness in European sea bass Dicentrarchus labrax [J].Aquaculture,2010,298:245-250.
[17] Costas B,Aragao C,Mancera J M,et al.High stocking density induces crowding stress and affects amino acid metabolism in Senegalese sole Solea senegalensis(Kaup 1858) juveniles [J].Aquacult Res,2008,39(1) :1-9.
[18] Yarahmadi P,Miandare H K,Fayaz S,et al.Increased stocking density causes changes in expression of selected stress-and immune-related genes,humoral innate immune parameters and stress responses of rainbow trout(Oncorhynchus mykiss) [J].Fish Shellfish Immunol,2016,48 :43-53.
[19] Procarione L S,Barry T P,Malison J A.Effects of high rearing densities and loading rates on the growth and stress responses of juvenile rainbow trout [J].N Am J Aquac,1999,61(2) :91-96.
[20] Ramsay J M,Feist G W,Varga Z M,et al.Whole-body cortisol is an indicator of crowding stress in adult zebrafish,Danio rerio [J].Aquaculture,2006,258 :565-574.
[21] 朱立新,侯刚,梁振林.基于贝叶斯方法的黄海北部鳀鱼体长与体重关系式参数估计[J].中国水产科学,2015,22(4) :757-769.
[22] Yoon H S.Length-weight relationships for 11 fishes caught by fish pots in the costal water off Baekya Island [J].Korean J Environ Biol,2013,31(2):154-157.
[2]范立民,徐跑,吴伟,等.淡水养殖池塘微生态环境调控研究综述[J].生态学杂志,2013,32(11) :3094-3100.
[3]Brown T W,Chappell J A,Boyd C E.A commercial-scale,in-pond raceway system for Ictalurid catfish production [J].Aquacult Eng,2011,44(3) :72-79.
[4]Naderi M,Keyvanshokooh S,Salati A P,et al.Combined or individual effects of dietary vitamin E and selenium nanoparticles on humoral immune status and serum parameters of rainbow trout(Oncorhynchus mykiss) under high stocking density[J].Aquaculture,2017,474 :40-47.
[5]Andrade T,Afonso A,Pérez-Jiménez A,et al.Evaluation of different stocking densities in a Senegalese sole(Solea senegalensis) farm:Implications for growth,humoral immune parameters and oxidative status [J].Aquaculture,2015,438:6-11.
[6] Di Marco P,Priori A,Finoia M G,et al.Physiological responses of European sea bass Dicentrarchus labrax to different stocking densities and acute stress challenge [J].Aquaculture,2008,275 :319-328.
[7]Ellis T,North B,Scott A P.Therelationships between stocking density and welfare in farmed rainbow trout [J].J Fish Biol,2002,61(3) :493-531.
[8] Millán-Cubillo A F,Martos-Sitcha J A,Ruiz-Jarabo I,et al.Low stocking density negatively affects growth,metabolism and stress pathways in juvenile specimens of meagre(Argyrosomus regius,Asso 1801) [J].Aquaculture,2016,451 :87-92.
[9]Pirozzi I,Booth M,Pankhurst P.The effect of stocking density and repeated handling on the growth of juvenile mulloway,Argyrosomus japonicus(Temminck & Schlegel 1843)[J].Aquac Int,2009,17(2):199-205.
[10]Liu B L,Jia R,Han C,et al.Effects of stocking density on antioxidant status,metabolism and immune response in juvenile turbot(Scophthalmus maximus) [J].Comp Biochem Phys C,2016,190 :1-8.
[11]Montero D,Izquierdo M S,Tort L,et al.High stocking density produces crowding stress altering some physiological and biochemical parameters in gilthead seabream,Sparus aurata,juveniles [J].Fish Physiol Biochem,1999,20(1) :53-60.
[12] Anderson D,Saoud I P,Davis D A.The effects of stocking density on survival,growth,condition,and feed efficiency of Bluegill juveniles [J].N Am J Aquac,2002,64(4) :297-300
[13]王炳谦,王芳,谷伟,等.不同养殖密度对褐鳟稚鱼生长性能的影响[J].东北农业大学学报,2014,45(12):18-23.
[14]Telli G S,Ranzani-Paiva M J T,Dias D C,et al.Dietary administration of Bacillus subtilis on hematology and non-specific immunity of Nile tilapia raised at different stocking densities [J].Fish Shellfish Immun,2014,39(2) :305-311.
[15]Garcia F,Romera D M,Gozi K S,et al.Stocking density of Nile tilapia in cages placed in a hydroelectric reservoir [J].Aquaculture,2013,410:51-56.
[16] Lupatsch I,Santos G A,Schrama J W,et al.Effect of stocking density and feeding level on energy expenditure and stress responsiveness in European sea bass Dicentrarchus labrax [J].Aquaculture,2010,298:245-250.
[17] Costas B,Aragao C,Mancera J M,et al.High stocking density induces crowding stress and affects amino acid metabolism in Senegalese sole Solea senegalensis(Kaup 1858) juveniles [J].Aquacult Res,2008,39(1) :1-9.
[18] Yarahmadi P,Miandare H K,Fayaz S,et al.Increased stocking density causes changes in expression of selected stress-and immune-related genes,humoral innate immune parameters and stress responses of rainbow trout(Oncorhynchus mykiss) [J].Fish Shellfish Immunol,2016,48 :43-53.
[19] Procarione L S,Barry T P,Malison J A.Effects of high rearing densities and loading rates on the growth and stress responses of juvenile rainbow trout [J].N Am J Aquac,1999,61(2) :91-96.
[20] Ramsay J M,Feist G W,Varga Z M,et al.Whole-body cortisol is an indicator of crowding stress in adult zebrafish,Danio rerio [J].Aquaculture,2006,258 :565-574.
[21] 朱立新,侯刚,梁振林.基于贝叶斯方法的黄海北部鳀鱼体长与体重关系式参数估计[J].中国水产科学,2015,22(4) :757-769.
[22] Yoon H S.Length-weight relationships for 11 fishes caught by fish pots in the costal water off Baekya Island [J].Korean J Environ Biol,2013,31(2):154-157.