新疆和田玫瑰花瓣的质量标准初步研究
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摘要
目的建立和田玫瑰花瓣药材质量标准,为控制该药材的质量提供实验依据。方法参照2015年版《中国药典》通则相关方法,对该药材总灰分、酸不溶性灰分和浸出物进行测定;采用薄层色谱法,以槲皮素为对照,对该药材进行定性鉴别;采用RT-HPLC法,以甲醇-0.1%磷酸(56∶44)为流动相,对玫瑰花瓣中的槲皮素含量进行测定。结果该药材薄层鉴别方法具有专属性,总灰分平均为4.9%,酸不溶性灰分平均为0.19%,浸出物为45.8%。槲皮素的含量平均值为0.21%。结论根据实验结果初步建立了和田玫瑰花药材的质量标准,所建立的质量标准可用于该产地玫瑰花瓣的质量控制。
Objective To establish a quality standard for rose petals of Hetian,and provide experimental basis for the quality control of the medicinal materials.Methods According to the general principles of Chinese pharmacopoeia in2015 the total ash content,acid insoluble ash and extract were determined.TLC method was used to identify the crude drug with quercetin as the reference.The content of quercetin in rose petals was determined by RT-HPLC with methanol-0.1% phosphoric acid(56∶44) as the mobile phase.Results The method of TLC had specificity,the total ash was 4.9% in average,the acid insoluble ash was 0.19% in average,and the extract was 45.8% in average.The average content of quercetin was 0.21%.Conclusion According to the experimental results,a quality standard for rose petals is established preliminarily,and the quality standard of the medicinal plants can be used for the quality control of rose petals of Hetian.
Objective To establish a quality standard for rose petals of Hetian,and provide experimental basis for the quality control of the medicinal materials.Methods According to the general principles of Chinese pharmacopoeia in2015 the total ash content,acid insoluble ash and extract were determined.TLC method was used to identify the crude drug with quercetin as the reference.The content of quercetin in rose petals was determined by RT-HPLC with methanol-0.1% phosphoric acid(56∶44) as the mobile phase.Results The method of TLC had specificity,the total ash was 4.9% in average,the acid insoluble ash was 0.19% in average,and the extract was 45.8% in average.The average content of quercetin was 0.21%.Conclusion According to the experimental results,a quality standard for rose petals is established preliminarily,and the quality standard of the medicinal plants can be used for the quality control of rose petals of Hetian.
引文
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[11]王斌利,王新娣,石晓峰,等.HPLC同时测定紫斑牡丹花粉中槲皮素、木犀草素、异鼠李素的含量[J].中国现代应用药学,2017,34(2):262-265.
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[18]李彩丽,平欲晖.HPLC测定芒萁中槲皮素和山柰素的含量[J].中国实验方剂学杂志,2015,21(19):64-66.
[19]刘桂花,邢建国,王云飞,等.HPLC法同时测定复方必清颗粒中秋水仙碱、槲皮素及薯蓣皂苷元[J].中成药,2015,37(7):1487-1491.
[20]俞静静,李风华,陈素红,等.HPLC同时测定不同产地马尾松和不同种属松叶中槲皮素、山柰酚的含量[J].药物分析杂志,2014,34(11):1969-1974.
[21]艾合买提·买买提,买合布白·阿不都热依木,艾克白尔·买买提,等.HPLC法测定玫瑰花瓣中槲皮素含量[J].药学进展,2010,34(5):228-230.
[22]苏思恩,阮焕群.金缕梅中槲皮素的提取工艺优化[J].广东化工,2017,44(8):84-85,107.
[23]闫利华,金艳,冯学峰,等.扶芳藤药材质量标准研究[J].中国中药杂志,2015,40(10):1877-1886.
[24]Mikanagi Y,Saito N,Yokoi M,et al.Anthocyanins in flowers of genus Rosa,sections Cinnamomeae(=Rosa),Chinenses,Gallicanae and some modern garden roses[J].Biochem Syst Ecol,2000,28(9):887-902.
[25]Ng TB,He JS,Niu SM,et al.A gallic acid derivative and polysaccharides with antioxidative activity from rose(Rosa rugosa)flowers[J].J Pharm Pharmacol,2004,56(4):537-545.
[2]贾佼佼,苗明三.玫瑰花的化学药理及应用分析[J].中医学报,2014,29(9):1337-1338,1350.
[3]马昌豪.玫瑰花化学成分与质量标准研究[D].济南:山东中医药大学,2012.
[4]张璐,季巧遇,吕红.玫瑰花概述及研究进展[J].江西中医药,2012,43(1):64-66.
[5]包满节,乌仁图雅,包苏布道.蒙药文冠木中槲皮素的质量标准研究[J].内蒙古医科大学学报,2010,32(6):554-557.
[6]郑筱萸.中药新药临床研究指导原则(试用版)[M].北京:中国医药科技出版社,2002:124.
[7]国家中医药管理局.中华本草[M].上海:上海科学技术出版社,1999:238-241.
[8]国家药典委员会.中国药典[M].北京:中国医药科技出版社,2015:204.
[9]王建平,汪铜芳,陈晓峰,等.HPLC法同时测定护肝颗粒中的槲皮素、山柰素和异鼠李素的含量[J].世界中医药,2017,12(4):903-905,908.
[10]曹晓华,沈旭斌,程先骄,等.HPLC双波长法测定豌豆尖中槲皮素和山奈酚的含量[J].食品科技,2017,42(4):270-276.
[11]王斌利,王新娣,石晓峰,等.HPLC同时测定紫斑牡丹花粉中槲皮素、木犀草素、异鼠李素的含量[J].中国现代应用药学,2017,34(2):262-265.
[12]张訸,刘云,罗旭璐,等.云南不同产地食用玫瑰花多酚含量及抗氧化活性[J].贵州农业科学,2017,45(2):150-153.
[13]陈吓俤,谢淑芳,郑小珍,等.金线莲中槲皮素的超声提取及HPLC法测定[J].湖北农业科学,2016,55(20):5324-5326.
[14]孙迪,付本宁,张志国.玫瑰花生物活性物质的研究进展[J].食品工业,2016,37(7):243-245.
[15]龚晓猛,张美,吴珊珊,等.山枝仁炒制前后槲皮素的含量测定及质量标准研究[J].中药与临床,2016,7(3):19-21.
[16]张慧月,耿晖,董琼,等.HPLC法同时测定山楂中芦丁和槲皮素含量[J].辽宁中医药大学学报,2016,18(1):64-66.
[17]常安,王维宁,杨燕云,等.HPLC同时测定辽宁省不同来源和产地透骨草中槲皮素和山柰酚的含量[J].中药材,2015,38(10):2053-2055.
[18]李彩丽,平欲晖.HPLC测定芒萁中槲皮素和山柰素的含量[J].中国实验方剂学杂志,2015,21(19):64-66.
[19]刘桂花,邢建国,王云飞,等.HPLC法同时测定复方必清颗粒中秋水仙碱、槲皮素及薯蓣皂苷元[J].中成药,2015,37(7):1487-1491.
[20]俞静静,李风华,陈素红,等.HPLC同时测定不同产地马尾松和不同种属松叶中槲皮素、山柰酚的含量[J].药物分析杂志,2014,34(11):1969-1974.
[21]艾合买提·买买提,买合布白·阿不都热依木,艾克白尔·买买提,等.HPLC法测定玫瑰花瓣中槲皮素含量[J].药学进展,2010,34(5):228-230.
[22]苏思恩,阮焕群.金缕梅中槲皮素的提取工艺优化[J].广东化工,2017,44(8):84-85,107.
[23]闫利华,金艳,冯学峰,等.扶芳藤药材质量标准研究[J].中国中药杂志,2015,40(10):1877-1886.
[24]Mikanagi Y,Saito N,Yokoi M,et al.Anthocyanins in flowers of genus Rosa,sections Cinnamomeae(=Rosa),Chinenses,Gallicanae and some modern garden roses[J].Biochem Syst Ecol,2000,28(9):887-902.
[25]Ng TB,He JS,Niu SM,et al.A gallic acid derivative and polysaccharides with antioxidative activity from rose(Rosa rugosa)flowers[J].J Pharm Pharmacol,2004,56(4):537-545.