摘要
人类线粒体N-乙酰氨基葡萄糖转移酶(mitochondrial O-GlcNAc transferse,m OGT)通过其N端基质导向序列(matrix targeting sequence,MTS)定位于线粒体内膜上,m OGT的过表达将导致细胞的凋亡。蛋白质的O-Glc NAc修饰存在于绝大多数真核生物中,而酿酒酵母唯独缺乏该修饰途径。对人体m OGT过表达引起酿酒酵母生长缺陷的机制进行分析,期望利用该模型研究m OGT导致的细胞凋亡调控机制。在酿酒酵母细胞中表达人体m OGT及其截短序列发现:m OGT对酿酒酵母的生长抑制及细胞中的定位依赖于N端全长的MTS序列; MTS序列在酿酒酵母中共表达导致线粒体融合。MTS序列过表达的酿酒酵母菌可以作为研究m OGT引起细胞凋亡的模式细胞。
Human mitochondrial O-GlcNAc transferase (mOGT) localized in mitochondria inner membrane through its N-terminal martrix targeting sequence (MTS),mOGT overexpression caused cell spoptosis. O-GlcNAc modification existed in most eukaryote cells except Saccharomyces cerevisiae. In order to analyze mOGT induced mammalian cell apoptosis,the mechanism of mOGT caused yeast cell growth defect was investigated. Herein,mOGT was overexpressed in Saccharomyces cerevisiae. Both yeast growth defect and mOGT localization were largely depends on MTS sequence. Furthermore,MTS expression caused yeast mitochondria fussion. Therefore,MTS overexpressed yeast cells might be applied to analyze mOGT caused cell apoptosis.
引文
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