光谱技术在食源性致病菌检测中的应用
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摘要
如何快速准确地对食源性致病菌进行检测,是控制食品安全问题的关键。传统的生化培养方法操作繁琐、费时费力,无法满足食源性致病菌快速检测的需求。随着光谱技术的发展,已经建立了不少快速、简便、特异、敏感、低耗的食源性致病菌检测新技术。阐述了食源性致病菌光谱分析的最新研究和发展趋势,展望了弹性散射光谱技术在单细胞水平实时、快速、无标记自动识别单个菌体细胞的应用,此平台的搭建将推动食源性致病菌无损检测技术的发展。
Quick and sensitive detection of foodborne pathogenic bacteria is a key to control food safety. Routine detection methods are so cumbersome and time-consuming that it is difficult to meet the needs of foodborne disease prevention and control. With the development of spectroscopic technologies, researchers have established many new techniques to detect bacteria. They are fast,simple, specific, sensitive, and with low-consumption. Latest spectroscopy research and development trends of foodborne pathogens have been summarized in this paper. It is proposed that single-cell level real-time and fast unmarked automatic identification of individual bacterial cells will be widely applied in the field of non-destructive testing of foodborne pathogenic bacteria.
Quick and sensitive detection of foodborne pathogenic bacteria is a key to control food safety. Routine detection methods are so cumbersome and time-consuming that it is difficult to meet the needs of foodborne disease prevention and control. With the development of spectroscopic technologies, researchers have established many new techniques to detect bacteria. They are fast,simple, specific, sensitive, and with low-consumption. Latest spectroscopy research and development trends of foodborne pathogens have been summarized in this paper. It is proposed that single-cell level real-time and fast unmarked automatic identification of individual bacterial cells will be widely applied in the field of non-destructive testing of foodborne pathogenic bacteria.
引文
[1]陆姣,王晓莉,吴林海.国内外食源性疾病防控的研究进展[J].中华疾病控制杂志,2017,21(2):196-199.
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[11]FAN W T,QIN T T,BI R R,et al.Performance of the matrix-assisted laser desorption ionization time-offlight mass spectrometry system for rapid identification of streptococci:a review[J].European Journal of Clinical Microbiology&Infectious Diseases,2017,36(6):1005-1012.
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[14]钟晓红.食品中致病菌快速检测方法的探讨[J].粮食流通技术,2016(24):59-60.
[15]MOKHTARZADEH A,DOLATABADI J E N,ABNOUS K,et al.Nanomaterial-based cocaine aptasensors[J].Biosensors&Bioelectronics,2015,68:95-106.
[16]B?HME K,BARROS-VELáZQUEZ J,CALO-MATA P.Chapter 2-Fingerprinting for detecting contaminants in food[M]//Food Protection and Security.Amsterdam:Elsevier,2017:15-42.
[17]冯明春,徐亮,金岭,等.傅里叶变换红外光谱仪动镜倾斜和动态校准研究[J].光子学报,2016,45(4):163-167.
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[19]WANG Y D,LI X L,LIU Z X,et al.Discrimination of foodborne pathogenic bacteria using synchrotron FTIRmicrospectroscopy[J].Nuclear Science and Techniques,2017,28(4):38-43.
[20]TOLEDO M,GUTIéRREZ M C,SILES J A,et al.Chemometric analysis and NIR spectroscopy to evaluate odorous impact during the composting of different raw materials[J].Journal of Cleaner Production,2017,167:154-162.
[21]BITTNER M,KR?HMER A,SCHENK R,et al.NIRspectroscopy of Actaea racemosa L.rhizome-en route to fast and low-cost quality assessment[J].Planta Medica,2017,83(12/13):1085-1096.
[22]ZHANG J,HOU Y H,CHANG X H,et al.Rapid detection of foodborne Vibrio parahaemolyticus,based on a near-infrared immunochromatographic technique[J].Modern Food Science&Technology,2017,33(5):282-287.
[23]XU Y,KUTSANEDZIE F Y H,SUN H,et al.Rapid Pseudomonas species identification from chicken by integrating colorimetric sensors with near-infrared spectroscopy[J].Food Analytical Methods,2018,11(4):1199-1208.
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[25]FLEISCHMANN M,HENDRA P J,MCQUILLAN AJ.Raman spectra of pyridine adsorbed at a silver electrode[J].Chemical Physics Letters,1974,26(2):163-166.
[26]POLISETTI S,BAIG N F,MORALESSOTO N,et al.Spatial mapping of pyocyanin in Pseudomonas aeruginosa bacterial communities using surface enhanced Raman scattering[J].Applied Spectroscopy,2017,71(2):215-223.
[27]GAO W,LI B,YAO R,et al.Intuitive label-free SERSdetection of bacteria using aptamer-based in situ Ag nanoparticles synthesis[J].Analytical Chemistry,2017,89(18):9836-9842.
[28]PEARSON B,WANG P,PANG S,et al.Innovative sandwich assay with dual optical and SERS sensing mechanisms for bacterial detection[J].AnalyticalMethods,2017,9:4732-4739.
[29]CHEN L,MUNGROO N,DAIKUARA L,et al.Labelfree NIR-SERS discrimination and detection of foodborne bacteria by in situ synthesis of Ag colloids[J].Journal of Nanobiotechnology,2015,13(1):1-9.
[30]MUNGROO N A,OLIVEIRA G,NEETHIRAJAN S.SERS based point-of-care detection of food-borne pathogens[J].Microchimica Acta,2016,183(2):697-707.
[31]MOSIERBOSS P A.Review on SERS of bacteria[J].Biosensors,2017,7(4):51.
[32]WANG T,ZENG L H,LI D L.A review on the methods for correcting the fluorescence inner-filter effect of fluorescence spectrum[J].Applied Spectroscopy Reviews,2017,52(10):883-908.
[33]SILVA A S,QUINTELAS C,FERREIRA E C,et al.Exploiting intrinsic fluorescence spectroscopy to discriminate between Acinetobacter calcoaceticusAcinetobacter baumannii complex species[J].RSCAdvances,2017,7(14):8581-8588.
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[35]蒋艳,徐溢,王人杰,等.新型纳米荧光探针在微流控细菌芯片检测中的应用[J].化学进展,2015,27(9):1240-1250.
[36]YANG D,ZHOU H,HAISCH C,et al.Reproducible E.coli detection based on label-free SERS and mapping[J].Talanta,2016,146:457-463.
[37]KAHRAMAN M,ZAMALEEVA A I,FAKHRULLINR F,et al.Layer-by-layer coating of bacteria with noble metal nanoparticles for surface-enhanced Raman scattering.[J].Analytical&Bioanalytical Chemistry,2009,395(8):2559-2567.
[38]ZHOU H,YANG D,IVLEVA N P,et al.SERSdetection of bacteria in water by in situ coating with Ag nanoparticles[J].Analytical Chemistry,2014,86(3):1525-1533.
[39]CHEN L,MUNGROO N,DAIKUARA L,et al.Labelfree NIR-SERS discrimination and detection of foodborne bacteria by in situ synthesis of Ag colloids[J].Journal of Nanobiotechnology,2015,13(1):1-9.
[40]NEUGEBAUER U,R?SCH P,POPP J.Raman spectroscopy towards clinical application:drug monitoring and pathogen identification[J].InternationalJournal of Antimicrobial Agents,2015,46:S35-S39.
[41]HU J J,CHAI Y.Blocking non-specific binding for phage-based magnetoelastic biosensors[J].Biosensors Journal,2015,4(2):130.
[42]HUANG X,XU Z,MAO Y,et al.Gold nanoparticlebased dynamic light scattering immunoassay for ultrasensitive detection of Listeria monocytogenes in lettuces.[J].Biosensors&Bioelectronics,2015,66:184-190.
[43]FRATAMICO P M,STROBAUGH T P,MEDINA MB,et al.Detection of Escherichia coli O157:H7 using a surface plasmon resonance biosensor[J].Biotechnology Techniques,1998,12(7):571-576.
[44]CHEN S,MOONEY M H,ELLIOTT C T,et al.Advances in surface plasmon resonance biosensor technology towards high-throughput,food-safety analysis[J].Trends in Analytical Chemistry,2010,29(11):1305-1315.
[45]PASHAZADEH P,MOKHTARZADEH A,HASANZADEH M,et al.Nano-materials for use in sensing of salmonella infections:recent advances[J].Biosensors&Bioelectronics,2017,87:1050-1064.
[46]NGUYEN H H,YI S Y,WOUBIT A,et al.A portable surface plasmon resonance biosensor for rapid detection of Salmonella typhimurium[J].Applied Science&Convergence Technology,2016,25(3):61-65.
[47]OH S Y,HEO N S,SHUKLA S,et al.Development of gold nanoparticle-aptamer-based LSPR sensing chips for the rapid detection of Salmonella typhimurium in pork meat[J].Scientific Reports,2017,7(1):10130.
[48]JO Y,JUNG J,KIM M H,et al.Label-free identification of individual bacteria using Fourier transform light scattering[J].Optics Express,2015,23(12):15792-15805.
[49]HUFFMAN D E,SEREBRENNIKOVA Y M,SMITHJ M,et al.Reagentless bacterial identification using a combination of multiwavelength transmission and angular scattering spectroscopy[J].Journal of Spectroscopy,2016,2016:5436821.
[50]周威,胡梁斌,李红波,等.食物中食源性病原菌检测技术研究进展[J].食品研究与开发,2017,38(9):213-216.
[51]WANG C,GUO X,FANG B,et al.Study of backscattering microspectrum for stomach cells at singlecell scale[J].Journal of Biomedical Optics,2010,15(4):040505.
[52]杨静,王成,谢成颖,等.基于主成分分析和反向传播神经网络的肝癌细胞后向散射显微光谱判别[J].生物医学工程学杂志,2017(2):246-252.
[53]蒋艳.基于纳米荧光探针的细菌芯片高效分析方法研究[D].重庆:重庆大学,2016.
[2]MACFADDEN D R,BOGOCH I I,ANDREWS J R.Advances in diagnosis,treatment,and prevention of invasive Salmonella infections[J].Current Opinion in Infectious Diseases,2016,29(5):453.
[3]李凡,许恒毅.不对称PCR技术及其在食源性致病菌检测中应用的研究进展[J].食品工业科技,2017,38(4):379-383.
[4]LIU D,YU Z,HUANG Y,et al.An immuno-magnetic nanobead probe competitive assay for rapid detection of Salmonella choleraesuis[J].Journal of Nanoscience&Nanotechnology,2016,16(3):2291.
[5]ZOU D C,CHANG S Y,RU Z,et al.Optimal conditions for rapid detection of Salmonella spp.via potassium manganese trifluoride nanoprobe-based nuclear magnetic resonance[J].Modern Food Science&Technology,2017,33(6):321-325.
[6]XIA S,YU Z,LIU D,et al.Developing a novelimmunochromatographic test strip with gold magnetic bifunctional nanobeads(GMBN)for efficient detection of Salmonella choleraesuis,in milk[J].Food Control,2016,59(1):507-512.
[7]谢雪钦,杨向莹,高静,等.近红外荧光探针及其在免疫分析中的应用[J].食品与生物技术学报,2015,34(3):225-231.
[8]杨春光,王宏伟,彭心婷,等.食品病原微生物快速检测技术研究进展[J].食品安全质量检测学报,2015,29(1):41-47.
[9]GIRARD V,MAILLER S,CHETRY M,et al.Identification and typing of the emerging pathogen Candida auris by matrix-assisted laser desorption ionisation time of flight mass spectrometry[J].Mycoses,2016,59(8):535-538.
[10]FALL B,LO C I,SAMB-BA B,et al.The ongoing revolution of MALDI-TOF mass spectrometry for microbiology reaches tropical Africa[J].American Journal of Tropical Medicine&Hygiene,2015,92(3):641-647.
[11]FAN W T,QIN T T,BI R R,et al.Performance of the matrix-assisted laser desorption ionization time-offlight mass spectrometry system for rapid identification of streptococci:a review[J].European Journal of Clinical Microbiology&Infectious Diseases,2017,36(6):1005-1012.
[12]NEVILLE S A,LECORDIER A,ZIOCHOS H,et al.Utility of matrix-assisted laser desorption ionizationtime of flight mass spectrometry following introduction for routine laboratory bacterial identification[J].Journal of Clinical Microbiology,2011,49(8):2980-2984.
[13]章钢刚,赖卫华.食源性致病菌免疫学检测方法研究进展[J].食品安全质量检测学报,2015(9):3414-3419.
[14]钟晓红.食品中致病菌快速检测方法的探讨[J].粮食流通技术,2016(24):59-60.
[15]MOKHTARZADEH A,DOLATABADI J E N,ABNOUS K,et al.Nanomaterial-based cocaine aptasensors[J].Biosensors&Bioelectronics,2015,68:95-106.
[16]B?HME K,BARROS-VELáZQUEZ J,CALO-MATA P.Chapter 2-Fingerprinting for detecting contaminants in food[M]//Food Protection and Security.Amsterdam:Elsevier,2017:15-42.
[17]冯明春,徐亮,金岭,等.傅里叶变换红外光谱仪动镜倾斜和动态校准研究[J].光子学报,2016,45(4):163-167.
[18]ZARNOWIEC P,MIZERA A,CHRAPEK M,et al.Chemometric analysis of attenuated total reflectance infrared spectra of Proteus mirabilis strains with defined structures of LPS[J].Innate Immunity,2016,22(5):325-335.
[19]WANG Y D,LI X L,LIU Z X,et al.Discrimination of foodborne pathogenic bacteria using synchrotron FTIRmicrospectroscopy[J].Nuclear Science and Techniques,2017,28(4):38-43.
[20]TOLEDO M,GUTIéRREZ M C,SILES J A,et al.Chemometric analysis and NIR spectroscopy to evaluate odorous impact during the composting of different raw materials[J].Journal of Cleaner Production,2017,167:154-162.
[21]BITTNER M,KR?HMER A,SCHENK R,et al.NIRspectroscopy of Actaea racemosa L.rhizome-en route to fast and low-cost quality assessment[J].Planta Medica,2017,83(12/13):1085-1096.
[22]ZHANG J,HOU Y H,CHANG X H,et al.Rapid detection of foodborne Vibrio parahaemolyticus,based on a near-infrared immunochromatographic technique[J].Modern Food Science&Technology,2017,33(5):282-287.
[23]XU Y,KUTSANEDZIE F Y H,SUN H,et al.Rapid Pseudomonas species identification from chicken by integrating colorimetric sensors with near-infrared spectroscopy[J].Food Analytical Methods,2018,11(4):1199-1208.
[24]穆渴心.基于傅里叶变换近红外光谱结合化学计量学的食源性致病菌快速检测[D].武汉:华中农业大学,2016.
[25]FLEISCHMANN M,HENDRA P J,MCQUILLAN AJ.Raman spectra of pyridine adsorbed at a silver electrode[J].Chemical Physics Letters,1974,26(2):163-166.
[26]POLISETTI S,BAIG N F,MORALESSOTO N,et al.Spatial mapping of pyocyanin in Pseudomonas aeruginosa bacterial communities using surface enhanced Raman scattering[J].Applied Spectroscopy,2017,71(2):215-223.
[27]GAO W,LI B,YAO R,et al.Intuitive label-free SERSdetection of bacteria using aptamer-based in situ Ag nanoparticles synthesis[J].Analytical Chemistry,2017,89(18):9836-9842.
[28]PEARSON B,WANG P,PANG S,et al.Innovative sandwich assay with dual optical and SERS sensing mechanisms for bacterial detection[J].AnalyticalMethods,2017,9:4732-4739.
[29]CHEN L,MUNGROO N,DAIKUARA L,et al.Labelfree NIR-SERS discrimination and detection of foodborne bacteria by in situ synthesis of Ag colloids[J].Journal of Nanobiotechnology,2015,13(1):1-9.
[30]MUNGROO N A,OLIVEIRA G,NEETHIRAJAN S.SERS based point-of-care detection of food-borne pathogens[J].Microchimica Acta,2016,183(2):697-707.
[31]MOSIERBOSS P A.Review on SERS of bacteria[J].Biosensors,2017,7(4):51.
[32]WANG T,ZENG L H,LI D L.A review on the methods for correcting the fluorescence inner-filter effect of fluorescence spectrum[J].Applied Spectroscopy Reviews,2017,52(10):883-908.
[33]SILVA A S,QUINTELAS C,FERREIRA E C,et al.Exploiting intrinsic fluorescence spectroscopy to discriminate between Acinetobacter calcoaceticusAcinetobacter baumannii complex species[J].RSCAdvances,2017,7(14):8581-8588.
[34]白冰,胡耀华,李敏通,等.量子点免疫标记法同时检测2种食源性致病菌[J].中国食品学报,2016,16(1):219-225.
[35]蒋艳,徐溢,王人杰,等.新型纳米荧光探针在微流控细菌芯片检测中的应用[J].化学进展,2015,27(9):1240-1250.
[36]YANG D,ZHOU H,HAISCH C,et al.Reproducible E.coli detection based on label-free SERS and mapping[J].Talanta,2016,146:457-463.
[37]KAHRAMAN M,ZAMALEEVA A I,FAKHRULLINR F,et al.Layer-by-layer coating of bacteria with noble metal nanoparticles for surface-enhanced Raman scattering.[J].Analytical&Bioanalytical Chemistry,2009,395(8):2559-2567.
[38]ZHOU H,YANG D,IVLEVA N P,et al.SERSdetection of bacteria in water by in situ coating with Ag nanoparticles[J].Analytical Chemistry,2014,86(3):1525-1533.
[39]CHEN L,MUNGROO N,DAIKUARA L,et al.Labelfree NIR-SERS discrimination and detection of foodborne bacteria by in situ synthesis of Ag colloids[J].Journal of Nanobiotechnology,2015,13(1):1-9.
[40]NEUGEBAUER U,R?SCH P,POPP J.Raman spectroscopy towards clinical application:drug monitoring and pathogen identification[J].InternationalJournal of Antimicrobial Agents,2015,46:S35-S39.
[41]HU J J,CHAI Y.Blocking non-specific binding for phage-based magnetoelastic biosensors[J].Biosensors Journal,2015,4(2):130.
[42]HUANG X,XU Z,MAO Y,et al.Gold nanoparticlebased dynamic light scattering immunoassay for ultrasensitive detection of Listeria monocytogenes in lettuces.[J].Biosensors&Bioelectronics,2015,66:184-190.
[43]FRATAMICO P M,STROBAUGH T P,MEDINA MB,et al.Detection of Escherichia coli O157:H7 using a surface plasmon resonance biosensor[J].Biotechnology Techniques,1998,12(7):571-576.
[44]CHEN S,MOONEY M H,ELLIOTT C T,et al.Advances in surface plasmon resonance biosensor technology towards high-throughput,food-safety analysis[J].Trends in Analytical Chemistry,2010,29(11):1305-1315.
[45]PASHAZADEH P,MOKHTARZADEH A,HASANZADEH M,et al.Nano-materials for use in sensing of salmonella infections:recent advances[J].Biosensors&Bioelectronics,2017,87:1050-1064.
[46]NGUYEN H H,YI S Y,WOUBIT A,et al.A portable surface plasmon resonance biosensor for rapid detection of Salmonella typhimurium[J].Applied Science&Convergence Technology,2016,25(3):61-65.
[47]OH S Y,HEO N S,SHUKLA S,et al.Development of gold nanoparticle-aptamer-based LSPR sensing chips for the rapid detection of Salmonella typhimurium in pork meat[J].Scientific Reports,2017,7(1):10130.
[48]JO Y,JUNG J,KIM M H,et al.Label-free identification of individual bacteria using Fourier transform light scattering[J].Optics Express,2015,23(12):15792-15805.
[49]HUFFMAN D E,SEREBRENNIKOVA Y M,SMITHJ M,et al.Reagentless bacterial identification using a combination of multiwavelength transmission and angular scattering spectroscopy[J].Journal of Spectroscopy,2016,2016:5436821.
[50]周威,胡梁斌,李红波,等.食物中食源性病原菌检测技术研究进展[J].食品研究与开发,2017,38(9):213-216.
[51]WANG C,GUO X,FANG B,et al.Study of backscattering microspectrum for stomach cells at singlecell scale[J].Journal of Biomedical Optics,2010,15(4):040505.
[52]杨静,王成,谢成颖,等.基于主成分分析和反向传播神经网络的肝癌细胞后向散射显微光谱判别[J].生物医学工程学杂志,2017(2):246-252.
[53]蒋艳.基于纳米荧光探针的细菌芯片高效分析方法研究[D].重庆:重庆大学,2016.