哮宁口服液对哮喘小鼠肺组织病理形态学的影响
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摘要
目的观察哮宁口服液对哮喘小鼠肺组织病理形态学变化的影响。方法 60只BALB/C雌性小鼠随机分为空白组、模型组、阳性组、中药组,采用卵清白蛋白与氢氧化铝致敏,并雾化吸入卵清蛋白激发制备哮喘模型。给药治疗7 d后取各组小鼠右肺组织,显微镜下行组织病理形态学观察。结果模型组、阳性组、中药组小鼠均不同程度地出现搔抓皮肤,呼吸加深加快,缩头弓背,毛发竖立等表现。给药治疗7 d后,阳性组、中药组小鼠上述症状和体征基本消失。病理形态学显示:模型组气管壁增厚、分泌物、肺泡破坏减少及炎性浸润最严重,中药组、阳性组炎性细胞浸润、气管分泌物均有所减轻。各组小鼠肺组织病变程度由轻到重依次为空白组<中药组、阳性组<模型组。结论哮宁口服液能改善哮喘小鼠模型的气道炎症,可能是其治疗哮喘的重要机制之一。
Objective To observe the influence on lung pathomorphology of mice with asthma treated with Xiaoning Koufuye.Methods 60 BALB/C female mouse were randomly divided into blank group,model group,positive group and Chinese medicine group. These all be made sensitization by OVA,and made aerosol inhalation of OVA to prepare to product asthma model.After treated for 7 days,the pathomorphological changes of right lung tissue of mouse were observed under microscope. Results Mouse in model,positive and Chinese medicine groups breathed faster with drop-head,hollow back and bristling hairs. After treated for 7 days,the body signs of mouse mentioned above in positive group and Chinese medicine group almost disappeared. In pathomorphology,it was reflected that wall thickened,secretion and pulmonary alveoli damage reduced and the inflammatory cell infiltration was the most serious in model group.But the inflammatory cell infiltration and trachea secretion in positive group and Chinese medicine group were both alleviated. The degree of pathological changes of mouse 'lung tissue in each group from light to heavy were blank group
Objective To observe the influence on lung pathomorphology of mice with asthma treated with Xiaoning Koufuye.Methods 60 BALB/C female mouse were randomly divided into blank group,model group,positive group and Chinese medicine group. These all be made sensitization by OVA,and made aerosol inhalation of OVA to prepare to product asthma model.After treated for 7 days,the pathomorphological changes of right lung tissue of mouse were observed under microscope. Results Mouse in model,positive and Chinese medicine groups breathed faster with drop-head,hollow back and bristling hairs. After treated for 7 days,the body signs of mouse mentioned above in positive group and Chinese medicine group almost disappeared. In pathomorphology,it was reflected that wall thickened,secretion and pulmonary alveoli damage reduced and the inflammatory cell infiltration was the most serious in model group.But the inflammatory cell infiltration and trachea secretion in positive group and Chinese medicine group were both alleviated. The degree of pathological changes of mouse 'lung tissue in each group from light to heavy were blank group
引文
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[10]李美容,王敏.卵清白蛋白复制哮喘动物模型的方法及评价研究进展[J].甘肃中医药大学学报,2016,33(1):71-74.
[2]姚力夫,陈虹,高颖,等.不同致敏方法对小鼠哮喘模型建立的影响[J].武警后勤学院学报(医学版),2016,25(2):97-100.
[3]邓学泉,姚银,郭洁波,等.Balb/c与DO11.10小鼠哮喘模型的建立[J].中国组织工程研究,2015,19(49):7 914-7 919.
[4]张方琪,韩新鹏,张芳,等.动态观察过敏原诱导的哮喘小鼠模型从急性炎症到慢性重塑的发展[J].中国病理生理杂志,2017,33(2):380-384.
[5]刘小凡,郭蓉晓,孟晓露,等.哮宁口服液治疗小儿哮喘的临床与实验研究[J].上海中医药杂志,2001,9(10):24-26.
[6]田金娜.哮宁口服液治疗支气管哮喘的实验研究[D].成都:成都中医药大学,2005.
[7]张舒锦,程媛,李涓,等.哮宁口服液治疗小儿哮喘的临床前研究[J].中药与临床,2011,27(2):126-128.
[8]刘佳,郑健,于涛.小鼠过敏性哮喘模型的研究进展及评价[J].中国比较医学杂志,2011,21(2):65-68.
[9]李美容,王敏.哮喘小鼠模型的建立与评价[J].临床肺科杂志,2011,16(5):664-665.
[10]李美容,王敏.卵清白蛋白复制哮喘动物模型的方法及评价研究进展[J].甘肃中医药大学学报,2016,33(1):71-74.