三丁酸甘油酯对乙酸刺激仔猪肠道屏障功能的影响
|
摘要
本试验旨在研究三丁酸甘油酯(TB)对乙酸(ACA)刺激仔猪小肠黏膜生长和肠道屏障功能的影响。试验选用18头体重接近、健康的28日龄断奶仔猪(杜×长×大),随机分为3个处理:对照组、乙酸组和TB组,每个处理6个重复,每个重复1头猪。试验期为21d。试验期内对照组和乙酸组饲喂基础日粮,TB组饲喂基础日粮+0.1%TB。于试验第15天清晨,乙酸组和TB组仔猪直肠灌注10mL 10%乙酸,对照组直肠灌注相应剂量的灭菌生理盐水。于试验第18、21天清晨前腔静脉采血,测定血浆二胺氧化酶(DAO)活力;第21天屠宰取样,取空肠、回肠等组织样品,测定仔猪肠道形态结构、肠道黏膜损伤相关基因mRNA水平等指标。结果表明:(1)未用乙酸刺激前,与对照组相比,日粮中添加0.1%TB对仔猪平均日增重有一定程度提高(P=0.089),且显著降低了仔猪料重比(P<0.05);(2)与对照组相比,乙酸组显著降低了空肠黏膜绒毛高度、回肠黏膜绒毛高度与隐窝深度比值及空肠绒毛表面积(P<0.05);(3)与乙酸组相比,TB组显著降低了血浆DAO活力(P<0.05),极显著降低了回肠AREG基因的相对表达量(P<0.01)。综合上述结果,日粮中添加0.1%TB能缓解乙酸诱导的仔猪肠黏膜生长及肠道屏障功能的损伤。
This study was conducted to study the effect of tributyrin(TB)on small intestine mucosal growth and gut barrier function of acetic acid(ACA)-treated piglets.Eighteen crossbred healthy piglets(Ducoc×Landrace×Yorkshire,28 days old)were randomly allocated into three treatment groups(control,ACA and TB).The pigs in control and ACA groups were fed basal diet,and TB group was fed the basal diet supplemented with 0.1% TB.On day 15 of the trial,ACA and TB groups were intracolonically administrated with 10 mL of 10% ACA solution,control group were given sterile saline at the same volume.On days 18 and 21,blood samples were collected from jugular vein for the determination of DAO activity.On day 21 of the trial,piglets were killed and the samples of small intestinal mucosa were collected for the measuring of intestinal morphological structure and mucosal injury correlated genes mRNA levels.The results showed that:(1)Before ACA administration,comparing to control group,dietary supplementation with TBcould increase ADG,but the difference was not significant(P=0.089),while reduced F/G significantly(P<0.05);(2)Compared with control group,ACA administration significantly decreased the villus height and surface area of villus in the jejunum,and the ratio of villus height to crypt depth in the ileum(P<0.05);(3)Compared with ACA group,TB supplementation significantly decreased the plasma DAO activity(P<0.05),and decreased the expression of AREGgene in the ileum mucosa extreme significantly(P<0.01).Collectively,dietary supplementation with 0.1%TB could alleviate ACA-induced damages on mucosal growth and barrier function in the small intestine of piglets.
This study was conducted to study the effect of tributyrin(TB)on small intestine mucosal growth and gut barrier function of acetic acid(ACA)-treated piglets.Eighteen crossbred healthy piglets(Ducoc×Landrace×Yorkshire,28 days old)were randomly allocated into three treatment groups(control,ACA and TB).The pigs in control and ACA groups were fed basal diet,and TB group was fed the basal diet supplemented with 0.1% TB.On day 15 of the trial,ACA and TB groups were intracolonically administrated with 10 mL of 10% ACA solution,control group were given sterile saline at the same volume.On days 18 and 21,blood samples were collected from jugular vein for the determination of DAO activity.On day 21 of the trial,piglets were killed and the samples of small intestinal mucosa were collected for the measuring of intestinal morphological structure and mucosal injury correlated genes mRNA levels.The results showed that:(1)Before ACA administration,comparing to control group,dietary supplementation with TBcould increase ADG,but the difference was not significant(P=0.089),while reduced F/G significantly(P<0.05);(2)Compared with control group,ACA administration significantly decreased the villus height and surface area of villus in the jejunum,and the ratio of villus height to crypt depth in the ileum(P<0.05);(3)Compared with ACA group,TB supplementation significantly decreased the plasma DAO activity(P<0.05),and decreased the expression of AREGgene in the ileum mucosa extreme significantly(P<0.01).Collectively,dietary supplementation with 0.1%TB could alleviate ACA-induced damages on mucosal growth and barrier function in the small intestine of piglets.
引文
[1]张文明,沈俊.溃疡性结肠炎的临床和内镜特点[J].中国临床医学,2010,13(4):214-215.ZHANG W M,SHEN J.Clinical analysis on the application of endoscopy in ulcerative colitis[J].Chinese Journal of Clinical Medicine,2010,13(4):214-215.(in Chinese)
[2]KORNBLUTH A,SACHAR D B.Ulcerative colitis practice guidelines in adults(update):American college of gastroenterology,practice parameters committee[J].The American Journal of Gastroenterology,2004,99:1371-1385.
[3]CETINKAYA A,BULBULOGLU E,KURUTAS E B,et al.Beneficial effects of N-acetylcysteine on acetic acid-induced colitis in rats[J].Tohoku,2005,206:131-139.
[4]WANG L,HOU Y Q,DAN Y,et al.Protective effects of N-acetylcysteine on acetic acid-induced colitis in a porcine model[J].BMC Gastroenterology,2013,13(1):1-11.
[5]SU J,ZHANG N N,HO P C.Determination of tributyrin and its metabolite butyrate in Wistar rat plasma samples by gas chromatography/mass spectrometry[J].Rapid Communications in Mass Spectrometry,2004,18(19):2217-2222.
[6]廖和菁,郑为完,易路遥,等.丁酸甘油酯的合成及其在油脂微囊化中的应用[J].食品科学,2009,30(16):203-205.LIAO H J,ZHENG W W,YI L Y,et al.Synthesis of glyceryl butyrate and its application in microencapsulation of soybean salad oil[J].Food Science,2009,30(16):203-205.(in Chinese)
[7]何进田,董丽,白凯文,等.三丁酸甘油脂对宫内发育迟缓哺乳仔猪血液生理生化指标和胰腺的影响[J].食品科学,2016,37(5):149-153.HE J T,DONG L,BAI K W,et al.Effect of tributyrin on blood physiobiochemical indices and pancrea of sucking piglets with intra-uterine growth retardation[J].Food Science,2016,37(5):149-153.(in Chinese)
[8]HOU Y Q,LIU Y L,HU J,et al.Effects of lactitol and tributyrin on growth performance,small intestinal morphology and enzyme activity in weaned pigs[J].Asian-Australasian Journal of Animal Sciences,2006,19:1470-1477.
[9]HOU Y Q,WANG L,YI D,et al.Dietary supplementation with tributyrin alleviates intestinal injury in piglets challenged with intrarectal administration of acetic acid[J].British Journal of Nutrition,2014,111(10):1748-1758.
[10]LEONEL A J,TEIXEIRA L G,OLIVEIRA R P,et al.Antioxidative and immunomodulatory effects of tributyrin supplementation on experimental colitis[J].British Journal of Nutrition,2013,109:1396-1407.
[11]PIVA A,PRANDINI A,FIORENTINI L,et al.Tributyrin and lactitol synergistically enhanced the trophic status of the intestinal mucosa and reduced histamine levels in the gut of nursery pigs[J].Journal of Animal Science,2002,80:670-680.
[12]SALMINEN S,BOULEY C,BOUTRON-RUAULT M C,et al.Functional food science and gastrointestinal physiology and function[J].British Journal of Nutrition,1998,80:147-171.
[13]HUDAUT S,GUIGNOT J,SERVIN A L.Escherichia coli strains colonising the gastrointestinal tract protect germfree mice against Salmonella Typhimurium infection[J].Gut,2001,49(1):47-55.
[14]JOHNSON L R,CHANDLER A M.RNA,DNA of gastric and duodenal mucosa in antractomized and gastrin-treated rats[J].American Journal of Physiology,1973,224(4):937-940.
[15]FRANKEL W L,ZHANG W,AFONSO J,et al.Glutamine enhancement of structure and function in transplanted small intestine in the rat[J].Journal of The American College of Nutrition,1993,17(1):47-55.
[16]YI D,HOU Y Q,WANG L,et al.Gene expression profiles in the intestine of Lipopolysaccharide challenged piglets[J].Frontiers in Bioscience-Landmark,2016,21(3):487-501.
[17]FU W J,STROMBERG A J,VIELE K,et al.Statistics and bioinformatics in nutritional sciences:analysis of complex date in the era of systems biology[J].Journal of Nutritional Biochemistry,2010,21(7):561-572.
[18]HANS U B.Methods of Enzymatic Analysis(Third Edition)[M].Florida:Basel,1983.
[19]刘玉兰,范伟,盂国权,等.罗格列酮对断奶仔猪肠黏膜结构和抗氧化能力的影响[J].中国畜牧杂志,2010,46(3):15-19.LIU Y L,FAN W,YU G Q,et al.Effect of rosiglitazone on weaned piglets intestinal morphology and antioxidant ability[J].Chinese Journal of Animal Science,2010,46(3):15-19.(in Chinese)
[20]芦娜,王恬,钟翔.不同的丁酸产品在猪禽生产中的应用研究进展[J].动物营养学报,2015,27(2):359-368.LU N,WANG T,ZHONG X.Research progress on the application of different butyric acid products in pig poultry production[J].Chinese Journal of Animal Science,2015,27(2):359-368.(in Chinese)
[21]PILLAI S B,HINMAN C E,LUQUETTE M H,et al.Heparin-binding epidermal growth factor-like growth factor protects rat intestine from ischemia/reperfusion injury[J].Journal of Surgical Research,1999,87(2):225-231.
[22]LIU F,YIN J,DU M,et al.Heat-stress-induced damage to porcine small intestinal epithelium associated with downregulation of epithelial growth factor signaling[J].Journal of Animal Science,2009,87:1941-1949.
[23]HELMRATH M A,SHIN C E,ERWIN C R.Intestinal adaptation is enhanced by epidermal growth factor independent of increased ileal epidermal growth factor receptor expression[J].Journal of Pediatric Surgrey,1998,33:980-985.
[24]NAIR R R,WARNER B B,WARNER B W.Role of epidermal growth factor and other growth factors in the prevention of necrotizing enterocolitis[J].Semin Perinatol,2008,32:107-113.
[25]SHOYAB M,PLOWMAN G D,MCDONALD V L,et al.Structure and function of human amphiregulin:A member of the epidermal growth factor family[J].Science,1989,243(4894):1074-1076.
[2]KORNBLUTH A,SACHAR D B.Ulcerative colitis practice guidelines in adults(update):American college of gastroenterology,practice parameters committee[J].The American Journal of Gastroenterology,2004,99:1371-1385.
[3]CETINKAYA A,BULBULOGLU E,KURUTAS E B,et al.Beneficial effects of N-acetylcysteine on acetic acid-induced colitis in rats[J].Tohoku,2005,206:131-139.
[4]WANG L,HOU Y Q,DAN Y,et al.Protective effects of N-acetylcysteine on acetic acid-induced colitis in a porcine model[J].BMC Gastroenterology,2013,13(1):1-11.
[5]SU J,ZHANG N N,HO P C.Determination of tributyrin and its metabolite butyrate in Wistar rat plasma samples by gas chromatography/mass spectrometry[J].Rapid Communications in Mass Spectrometry,2004,18(19):2217-2222.
[6]廖和菁,郑为完,易路遥,等.丁酸甘油酯的合成及其在油脂微囊化中的应用[J].食品科学,2009,30(16):203-205.LIAO H J,ZHENG W W,YI L Y,et al.Synthesis of glyceryl butyrate and its application in microencapsulation of soybean salad oil[J].Food Science,2009,30(16):203-205.(in Chinese)
[7]何进田,董丽,白凯文,等.三丁酸甘油脂对宫内发育迟缓哺乳仔猪血液生理生化指标和胰腺的影响[J].食品科学,2016,37(5):149-153.HE J T,DONG L,BAI K W,et al.Effect of tributyrin on blood physiobiochemical indices and pancrea of sucking piglets with intra-uterine growth retardation[J].Food Science,2016,37(5):149-153.(in Chinese)
[8]HOU Y Q,LIU Y L,HU J,et al.Effects of lactitol and tributyrin on growth performance,small intestinal morphology and enzyme activity in weaned pigs[J].Asian-Australasian Journal of Animal Sciences,2006,19:1470-1477.
[9]HOU Y Q,WANG L,YI D,et al.Dietary supplementation with tributyrin alleviates intestinal injury in piglets challenged with intrarectal administration of acetic acid[J].British Journal of Nutrition,2014,111(10):1748-1758.
[10]LEONEL A J,TEIXEIRA L G,OLIVEIRA R P,et al.Antioxidative and immunomodulatory effects of tributyrin supplementation on experimental colitis[J].British Journal of Nutrition,2013,109:1396-1407.
[11]PIVA A,PRANDINI A,FIORENTINI L,et al.Tributyrin and lactitol synergistically enhanced the trophic status of the intestinal mucosa and reduced histamine levels in the gut of nursery pigs[J].Journal of Animal Science,2002,80:670-680.
[12]SALMINEN S,BOULEY C,BOUTRON-RUAULT M C,et al.Functional food science and gastrointestinal physiology and function[J].British Journal of Nutrition,1998,80:147-171.
[13]HUDAUT S,GUIGNOT J,SERVIN A L.Escherichia coli strains colonising the gastrointestinal tract protect germfree mice against Salmonella Typhimurium infection[J].Gut,2001,49(1):47-55.
[14]JOHNSON L R,CHANDLER A M.RNA,DNA of gastric and duodenal mucosa in antractomized and gastrin-treated rats[J].American Journal of Physiology,1973,224(4):937-940.
[15]FRANKEL W L,ZHANG W,AFONSO J,et al.Glutamine enhancement of structure and function in transplanted small intestine in the rat[J].Journal of The American College of Nutrition,1993,17(1):47-55.
[16]YI D,HOU Y Q,WANG L,et al.Gene expression profiles in the intestine of Lipopolysaccharide challenged piglets[J].Frontiers in Bioscience-Landmark,2016,21(3):487-501.
[17]FU W J,STROMBERG A J,VIELE K,et al.Statistics and bioinformatics in nutritional sciences:analysis of complex date in the era of systems biology[J].Journal of Nutritional Biochemistry,2010,21(7):561-572.
[18]HANS U B.Methods of Enzymatic Analysis(Third Edition)[M].Florida:Basel,1983.
[19]刘玉兰,范伟,盂国权,等.罗格列酮对断奶仔猪肠黏膜结构和抗氧化能力的影响[J].中国畜牧杂志,2010,46(3):15-19.LIU Y L,FAN W,YU G Q,et al.Effect of rosiglitazone on weaned piglets intestinal morphology and antioxidant ability[J].Chinese Journal of Animal Science,2010,46(3):15-19.(in Chinese)
[20]芦娜,王恬,钟翔.不同的丁酸产品在猪禽生产中的应用研究进展[J].动物营养学报,2015,27(2):359-368.LU N,WANG T,ZHONG X.Research progress on the application of different butyric acid products in pig poultry production[J].Chinese Journal of Animal Science,2015,27(2):359-368.(in Chinese)
[21]PILLAI S B,HINMAN C E,LUQUETTE M H,et al.Heparin-binding epidermal growth factor-like growth factor protects rat intestine from ischemia/reperfusion injury[J].Journal of Surgical Research,1999,87(2):225-231.
[22]LIU F,YIN J,DU M,et al.Heat-stress-induced damage to porcine small intestinal epithelium associated with downregulation of epithelial growth factor signaling[J].Journal of Animal Science,2009,87:1941-1949.
[23]HELMRATH M A,SHIN C E,ERWIN C R.Intestinal adaptation is enhanced by epidermal growth factor independent of increased ileal epidermal growth factor receptor expression[J].Journal of Pediatric Surgrey,1998,33:980-985.
[24]NAIR R R,WARNER B B,WARNER B W.Role of epidermal growth factor and other growth factors in the prevention of necrotizing enterocolitis[J].Semin Perinatol,2008,32:107-113.
[25]SHOYAB M,PLOWMAN G D,MCDONALD V L,et al.Structure and function of human amphiregulin:A member of the epidermal growth factor family[J].Science,1989,243(4894):1074-1076.