铈掺量对羟基磷灰石结构及吸附性能的影响
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摘要
羟基磷灰石(HAp)由于其良好的生物相容性和生物活性,而在生物医学方面具有广泛的应用.为了寻找更好的生物载体材料,采用湿化学沉淀法制备出了不同剂量(0mol%、2mol%、4mol%、6 mol%、8 mol%、10 mol%)铈掺杂的羟基磷灰石材料(CeHAp),并通过XRD、FTIR、HRTEM、XPS等对样品进行结构和化学分析.同时,还研究了CeHAp对牛血清蛋白(BSA)的吸附性能.研究结果表明,随着铈掺量的增加,样品的结晶度降低、晶粒尺寸减小;当Ce~(3+)和Ce~(4+)被掺入HAp中时,BSA吸附性随着铈掺量的增加而增加.
Hydroxyapatite is widely used in biomedicine because of its good biocompatibility and biological activity.In order to find better biomaterials,different doping amounts(0 mol%,2 mol%,4 mol%,6 mol%,8 mol%,10 mol%)of cerium(Ce)were doped into HAp by wet chemical method.The structure and chemical analysis of the samples were characterized by XRD,FTIR,HRTEM,XPS and so on.Moreover,the bovine serum albumin(BSA)adsorption of the samples was evaluated.The results indicated that the crystallinity and crystallite size of the samples decreased with the increase doping amount of cerium.Ce~(3+) and Ce~(4+) have been incorporated into the HAp lattice,and the BSA adsorption properties of CeHAp particles were found to increase with increasing cerium content.
Hydroxyapatite is widely used in biomedicine because of its good biocompatibility and biological activity.In order to find better biomaterials,different doping amounts(0 mol%,2 mol%,4 mol%,6 mol%,8 mol%,10 mol%)of cerium(Ce)were doped into HAp by wet chemical method.The structure and chemical analysis of the samples were characterized by XRD,FTIR,HRTEM,XPS and so on.Moreover,the bovine serum albumin(BSA)adsorption of the samples was evaluated.The results indicated that the crystallinity and crystallite size of the samples decreased with the increase doping amount of cerium.Ce~(3+) and Ce~(4+) have been incorporated into the HAp lattice,and the BSA adsorption properties of CeHAp particles were found to increase with increasing cerium content.
引文
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[3]程瑶,王铭,王星星,等.仿骨成分多元掺杂羟基磷灰石体外成骨性能研究[J].无机材料学报,2016,31(12):1 341-1 346.
[4]Ruphuy G,Saralegi A,Lopes J C,et al.Spray drying as a viable process to produce nano-hydroxyapatite/chitosan(n-HAp/CS)hybrid microparticles mimicking bone composition[J].Advanced Powder Technology,2016,27(2):575-583.
[5]You B,Li Q,Dong H,et al.Bilayered HA/CS/PEGDA hydrogel with good biocompatibility and self-healing property for potential application in osteochondral defect repair[J].Journal of Materials Science&Technology,2018,34(6):1 016-1 025.
[6]Fihri A,Len C,Varma R S,et al.Hydroxyapatite:A review of syntheses,structure and applications in heterogeneous catalysis[J].Coordination Chemistry Reviews,2017,347:48-76.
[7]Fakharzadeh A,Ebrahimi Kahrizsangi R,Nasiri Tabrizi B,et al.Effect of dopant loading on the structural features of silver-doped hydroxyapatite obtained by mechanochemical method[J].Ceramics International,2017,43(15):12 588-12 598.
[8]A.Bootchanont,W.Sailuam,S.Sutikulsombat,et al.Synchrotron X-ray absorption spectroscopy study of local structure in strontium-doped hydroxyapatite[J].Ceramics International,2017,43(14):11 023-11 027.
[9]Stipniece L,Stepanova V,Narkevica I,et al.Comparative study of surface properties of Mg-substituted hydroxyapatite bioceramic microspheres[J].Journal of the European Ceramic Society,2017,38(2):761-768.
[10]Alili L,Sack M,Karakoti A S,et al.Combined cytotoxic and anti-invasive properties of redox-active nanoparticles in tumor-stroma interactions[J].Biomaterials,2011,32(11):2 918-2 929.
[11]Yuan Q,Qin C,Wu J,et al.Synthesis and characterization of Cerium-doped hydroxyapatite/polylactic acid composite coatings on metal substrates[J].Materials Chemistry&Physics,2016,182:365-371.
[12]Y.Lin,Z.Yang,J.Cheng.Preparation,characterization and antibacterial property of cerium substituted hydroxyapatite nanoparticles[J].Journal of Rare Earths,2007,25(4):452-456.
[13]Kanchana P,Navaneethan M,Sekar C.Fabrication of Ce doped hydroxyapatite nanoparticles based non-enzymatic electrochemical sensor for the simultaneous determination of norepinephrine,uric acid and tyrosine[J].Materials Science&Engineering:B,2017,226:132-140.
[14]黄勇,王迎军,宁成云,等.微弧氧化法制备含铈羟基磷灰石生物活性膜层[J].材料科学与工程学报,2008,26(1):80-82.
[15]Priyadarshini.B,Anjaneyulu.U,Vijayalakshmi.U.Preparation and characterization of sol-gel derived Ce4+doped hydroxyapatite and its in vitro biological evaluations for orthopedic applications[J].Materials&Design,2017,119:446-455.
[16]Gopi D,Ramya S,Rajeswari D,et al.Corrosion protection performance of porous strontium hydroxyapatite coating on polypyrrole coated 316Lstainless steel[J].Colloids&Surfaces B Biointerfaces,2013,107(4):130-136.
[17]Baradaran S,Nasiri Tabrizi B,Shirazi F S,et al.Wet chemistry approach to the preparation of tantalumdoped hydroxyapatite:Dopant content effects[J].Ceramics International,2017,44(3):2 768-2 781.
[18]Bêche E,Charvin P,Perarnau D,et al.Ce 3dXPS investigation of cerium oxides and mixed cerium oxide(CexTiyOz)[J].Surface&Interface Analysis,2008,40(3-4):264-267.
[19]Ciobanu G,Bargan A M,Luca C.New cerium(IV)-substituted hydroxyapatite nanoparticles:Preparation and characterization[J].Ceramics International,2015,41(9):12 192-12 201.
[20]Z.Chen,Y.Liu,L.Mao,et al.Effect of cation doping on the structure of hydroxyapatite and the mechanism of defluoridation[J].Ceramics International,2018,44(6):6 002-6 009.
[21]Dasgupta S,Banerjee S S,Bandyopadhyay A,et al.Znand Mg-doped hydroxyapatite nanoparticles for controlled release of protein[J].Langmuir the Acs Journal of Surfaces&Colloids,2010,26(7):4 958-4 964.
[22]Xu Y,An L,Chen L,et al.Controlled hydrothermal synthesis of strontium-substituted hydroxyapatite nanorods and their application as a drug carrier for proteins[J].Advanced Powder Technology,2018,29(4):1 042-1 048.