低浓度饮水型砷暴露人群皮肤损伤的影响因素分析
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摘要
目的探讨低浓度饮水型砷暴露人群皮肤损伤的影响因素。方法在山西省大同市,选择85名地方性砷中毒(简称地砷病)病区(水砷含量为14.41~90.34μg/L)某村饮水年限20年以上本地居民,无传染性、遗传性等疾病,无放射线和理化致病因素接触史者为调查对象。按照皮肤损伤程度将调查对象分为无皮肤损伤组(即正常组)、轻度皮肤损伤组(简称轻度皮损组)、中度皮肤损伤组(简称中度皮损组)和重度皮肤损伤组(简称重度皮损组)。原子荧光光谱法测定研究对象体内尿砷水平,ELISA试剂盒测定其血清8-OHdG的含量。结果不同皮损组的尿砷和血清8-OHdG分布差异均有统计学意义(P<0.05)。重度皮损组的尿砷含量高于正常组和轻度皮损组(P<0.05);轻度、中度和重度皮损组的血清8-OHdG含量均高于正常组(P<0.05),且重度皮损组的血清8-OHdG含量高于轻度和中度皮损组(P<0.05)。Logistic回归分析显示,年龄和血清8-OHdG是砷暴露人群皮肤损伤的主要危险因素(OR=9.87,P<0.05;OR=6.08,P<0.05)。结论砷暴露所致的皮肤损伤可能与暴露时间和氧化损伤关系密切。
Objective To explore the influencing factors of skin lesion in people exposed to low concentration arsenic in drinking water. Methods A total of 85 people were selected from arsenic exposure area in Datong city,Shanxi province(the arsenic content in drinking water was 14.41-90.34μg/L)as the research subjects(the people living in the arsenic exposure area and drinking local water for more than twenty years consecutively).All of the candidates had neither infectious nor genetic diseases nor contacted radiation or physicochemic factors which may cause disease.The study subjects were divided into four groups:none skin lesion group(normal group),mild skin lesion group,moderate skin lesion group)and severe skin lesion group,based on the degree of skin lesion.Urine arsenic concentration was detected by Hydride Generation Atomic Absorption Spectroscopy.Serum 8-hydroxy-2'-deoxyguanosine(8-OHdG)level was analyzed by the Enzyme-linked Immunosorbent Assay(ELISA)kit. Results There were significant differences in urine arsenic and serum8-OHdG levels among groups(P <0.05).The concentration of urine arsenic in severe skin lesion group was significantly higher than that in normal and mild skin lesion groups(P <0.05).The content of serum 8-OHdG in mild,moderate and severe skin lesion groups were obviously higher than that in normal group(P <0.05),and the content of serum 8-OHdG in severe skin lesion group was markedly higher than that in mild and moderate skin lesion groups(P <0.05).Logistic regression analysis showed that age and serum 8-OHdG were major risk factors for skin lesion that caused by arsenic exposure.(OR was 9.87,P <0.05 and OR was 6.08,P <0.05). Conclusion Skin lesion caused by arsenic exposure may has a close correlation with exposure time and oxidative damage.
Objective To explore the influencing factors of skin lesion in people exposed to low concentration arsenic in drinking water. Methods A total of 85 people were selected from arsenic exposure area in Datong city,Shanxi province(the arsenic content in drinking water was 14.41-90.34μg/L)as the research subjects(the people living in the arsenic exposure area and drinking local water for more than twenty years consecutively).All of the candidates had neither infectious nor genetic diseases nor contacted radiation or physicochemic factors which may cause disease.The study subjects were divided into four groups:none skin lesion group(normal group),mild skin lesion group,moderate skin lesion group)and severe skin lesion group,based on the degree of skin lesion.Urine arsenic concentration was detected by Hydride Generation Atomic Absorption Spectroscopy.Serum 8-hydroxy-2'-deoxyguanosine(8-OHdG)level was analyzed by the Enzyme-linked Immunosorbent Assay(ELISA)kit. Results There were significant differences in urine arsenic and serum8-OHdG levels among groups(P <0.05).The concentration of urine arsenic in severe skin lesion group was significantly higher than that in normal and mild skin lesion groups(P <0.05).The content of serum 8-OHdG in mild,moderate and severe skin lesion groups were obviously higher than that in normal group(P <0.05),and the content of serum 8-OHdG in severe skin lesion group was markedly higher than that in mild and moderate skin lesion groups(P <0.05).Logistic regression analysis showed that age and serum 8-OHdG were major risk factors for skin lesion that caused by arsenic exposure.(OR was 9.87,P <0.05 and OR was 6.08,P <0.05). Conclusion Skin lesion caused by arsenic exposure may has a close correlation with exposure time and oxidative damage.
引文
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[13]李昕,李冰,郑全美,等.燃煤型砷中毒患者症状分级和尿砷水平与尿中8-羟基脱氧鸟嘌呤核苷水平的相关性研究[J].环境与健康杂志,2010,27(l2):1 038-1 039.
[14]WS/T 211-2001地方性砷中毒诊断标准[S].北京:中华人民共和国卫生部,2001.
[15]Cooper KL,King BS,Sandoval MM,et al.Reduction of arseniteenhanced ultraviolet radiation-induced DNA damage by supplemental zinc[J].Toxicol Appl Pharmacol,2013,269(2):81-88.
[16]徐苑苑,王毅,李昕,等.慢性饮水型砷暴露对人体DNA氧化损伤的影响[J].中国地方病学杂志,2008,27(4):382-384.
[2]郑晓南,李瑞,王智勇,等.2011~2012年大连市市售食品中铅砷铝含量调查[J].预防医学论坛,2015,21(4):244-245.
[3]Pei Q,Ma N,Zhang J,et al.Oxidative DNA damage of peripheral blood polymorphonuclear leukocytes,selectively induced by chronic arsenic exposure,is associated with extent of arsenic-related skin lesions[J].Toxicol Appl Pharmacol,2013,266(1):143-149.
[4]Guo X,Liu Z,Huang C,et al.Levels of arsenic in drinking-water and cutaneous lesions in Inner Mongolia[J].J Health Popu1Nutr,2006,24(2):214-220.
[5]Guo X,Fujino Y,Ye X,et al.Association between multi-level inorganic arsenic exposure from drinking water and skin lesions in China[J].Int J Environ Res Public Health,2006,3(3):262-267.
[6]赵灿方,康立,李文嘉,等.原子荧光光谱法同时测定水产品中砷和硒[J].预防医学论坛,2015,21(2):123-125,129.
[7]杨克敌.环境卫生学[M].第6版.北京:人民卫生出版社,2007:244-253.
[8]毛广运,杨佐鹏,任春生,等.环境砷中毒人群皮肤损害与DNA氧化损伤的关联性[J].中国环境科学,2010,30(1):99-103.
[9]Wu LL,Chiou CC,Chang PY,et al.Urinary 8-OHdG:a marker of oxidative stress to DNA and a risk factor for cancer,atherosclerosis anddiabetics[J].Clin Chim Acta,2004,339(1-2):1-9.
[10]Hinhumpatch P,Navasumrit P,Chaisatra K,et al.Oxidative DNA damage and repair in children exposed to low levels of arsenic in utero and during early childhood:application of salivary and urinary biomarkers[J].Toxicol Appl Pharmacol,2013,273(3):569-579.
[11]马艳,郑玉建,吴顺华.砷对神经系统损伤的研究进展[J].中国地方病学杂志,2007,26(1):111-113.
[12]徐文超,李勇,李云云,等.低浓度砷暴露者皮肤损害及DNA氧化损伤[J].中国公共卫生,2013,29(4):573-575.
[13]李昕,李冰,郑全美,等.燃煤型砷中毒患者症状分级和尿砷水平与尿中8-羟基脱氧鸟嘌呤核苷水平的相关性研究[J].环境与健康杂志,2010,27(l2):1 038-1 039.
[14]WS/T 211-2001地方性砷中毒诊断标准[S].北京:中华人民共和国卫生部,2001.
[15]Cooper KL,King BS,Sandoval MM,et al.Reduction of arseniteenhanced ultraviolet radiation-induced DNA damage by supplemental zinc[J].Toxicol Appl Pharmacol,2013,269(2):81-88.
[16]徐苑苑,王毅,李昕,等.慢性饮水型砷暴露对人体DNA氧化损伤的影响[J].中国地方病学杂志,2008,27(4):382-384.