貂源肺炎克雷伯氏菌的分离及毒力和耐药性的分析
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摘要
为探讨采自山东省部分地区的10株貂源肺炎克雷伯氏菌(Kp)的毒力和耐药性,本研究从疑似肺炎发病水貂的肺中分离出10株革兰阴性菌,经PCR及Vitek全自动细菌鉴定仪鉴定为肺炎克雷伯氏菌。对10株肺炎克雷伯氏菌进行人工感染及毒力试验、药敏试验、耐药基因检测及接合转移试验。结果显示,10株肺炎克雷伯氏菌攻毒后小鼠全部死亡,肺、肝、脾和肾均有不同程度的出血。分离株中大部分肺炎克雷伯氏菌对临床常用药物表现出多重耐药,其中对氨基糖苷类、青霉素类、三代头孢菌素类、喹诺酮类及磺胺类抗生素的耐药性较高,但对阿莫西林/克拉维酸、亚胺培南及多黏菌素等抗生素敏感。耐药基因TEM、SHV和CTX-M的检出率分别为100%、70%和80%,以TEM基因检出率最高。
The purpose of this study was to investigate the virulence and drug resistance of Klebsiella pneumoniae from 10 mink sources in some areas of Shandong Province,In this study,10 strains of Gram-negative bacteria were isolated from mink lungs suspected of suffering from pneumonia.It was belonged to Klebsiella pneumoniae by the determination of PCR and Vitek Automated Bacteria Identification instrument.10 strains of Klebsiella pneumoniae isolates from mink were carried out on artificial infection,virulence test,drug sensitivity test,detection and transferation of drug resistance gene.In result,the infected mice died 100%,the lung,liver,spleen and kidney showed hemorrhages to different degree.Most Klebsiella pneumoniae isolates have multiple drug resistance.They had higher resistance to ampicillin,chloramphenicol,kanamycin,tetracycline and sulfamethoxazole/trimetho-prim,and sensitive to antibiotics such as amoxicillin/clavic acid,imipenem and polymyxin.The detection rates of TEM,SHV and CTX-M were 100%,70% and 80%,respectively.The highest detection rate of TEM gene.
The purpose of this study was to investigate the virulence and drug resistance of Klebsiella pneumoniae from 10 mink sources in some areas of Shandong Province,In this study,10 strains of Gram-negative bacteria were isolated from mink lungs suspected of suffering from pneumonia.It was belonged to Klebsiella pneumoniae by the determination of PCR and Vitek Automated Bacteria Identification instrument.10 strains of Klebsiella pneumoniae isolates from mink were carried out on artificial infection,virulence test,drug sensitivity test,detection and transferation of drug resistance gene.In result,the infected mice died 100%,the lung,liver,spleen and kidney showed hemorrhages to different degree.Most Klebsiella pneumoniae isolates have multiple drug resistance.They had higher resistance to ampicillin,chloramphenicol,kanamycin,tetracycline and sulfamethoxazole/trimetho-prim,and sensitive to antibiotics such as amoxicillin/clavic acid,imipenem and polymyxin.The detection rates of TEM,SHV and CTX-M were 100%,70% and 80%,respectively.The highest detection rate of TEM gene.
引文
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[14]卓超,苏丹虹,倪语星,等.2010年CHINET克雷伯氏菌属细菌耐药性监测[J].中国感染与化疗杂志,2012,12(3):174-179.ZHUO Chao,SU Dan-hong,NI Yu-xing,et al.CHINETsurveillance of antimicrobial resistance in Klebsiella spp.during 2010[J].Chinese Journal of Infection and Chemotherapy,2012,12(3):174-179.(in Chinese)
[2]CRAVEN D E.What is healthcare-associated pneumonia,and how should it be treated?[J].Current Opinionin Infectious Diseases,2006,19(2):153-160.
[3]ROSENBLUM R,KHAN E,GONZALEZ G,et al.Genetic regulation of the ram A locus and its expression in clinical isolates of Klebsiella pneumoniae[J].International Journal of Antimicrobial Agents,2011,38(1):39-45.
[4]ASLAN V,MADEN M,ERGANIS O,et al.Clinical efficacy of florfenicol in the treatment of calf respiratory tract infections[J].Veterinary Quarterly,2002,24(1):35-39.
[5]唐曙明,方红辉,杨自华.产ESBLs肺炎克雷伯菌的耐药型与基因分型[J].中国现代医学杂志,2009,19(11):1653-1657.TANG Shu-ming,FANG Hong-hui,YANG Zi-hua.Relationship between the drug-resistance phenotype and its genotype of the extended spectrumβ-lactamase Klebsiella pneumoniae[J].China Journal of Modern Medicine,2009,19(11):1653-1657.(in Chinese)
[6]KOREN J,VACULIKOVA A.Development ofβ-lactamase resistance in enterobacteria[J].Klinicka Mikrobiologie a Infekcni Lekarstvi,2006,12(3):103-107.
[7]杨艳玲,陈峙峰,王成福,等.水貂肺炎克雷伯氏菌的分离鉴定[J].特产研究,2012,34(4):10-12.YANG Yan-ling,CHEN Zhi-feng,WANG Cheng-fu,et al.Isolation and identification on Klebsiella peneumoniae in minks[J].Special Wild Economic Animal and Plant Research,2012,34(4):10-12.(in Chinese)
[8]NEUBERGER A,OREN I,SPRECHER H.Clinical impact of a PCR assay for rapid identification of Klebsiella pneumoniae in blood cultures[J].Journal of Clinical Microbiology,2008,46(1):377-379.
[9]NEWIRE E A,AHMED S F,HOUSE B,et al.Detection of new SHV-12,SHV-5 and SHV-2a variants of extended spectrumβ-lactamase in Klebsiella pneumonia in Egypt[J].Annals of Clinical Microbiology and Antimicrobials,2013,12(1):1-12.
[10]DU P C,ZHANG Y Y,CHEN C.Emergence of carbapenemresistant hypervirulent Klebsiella pneumoniae[J].Lancet Infectious Diseases,2018,18(1):23-24.
[11]FERTAS-AISSANI R E,MESSAI Y,ALOUACHE S,et al.Virulence profiles and antibiotic susceptibility patterns of Klebsiella pneumoniae strains isolated from different clinical specimens[J].Pathologie Biologie,2013,61(5):209-216.
[12]PIPERAKI E T,SYROGIANNOPOULOS G A,TZOUVELEKIS LS,et al.Klebsiella pneumoniae:virulence,biofilm and antimicrobial resistance[J].The Pediatric Infectious Disease Journal,2017,36(10):1-14.
[13]MANSOUR W,HAENNI M,SARAS E,et al.Outbreak of colistin-resistant carbapenemase-producing Klebsiella pneumoniae in Tunisia[J].Journal of Global Antimicrobial Resistance,2017,10(16):88-94.
[14]卓超,苏丹虹,倪语星,等.2010年CHINET克雷伯氏菌属细菌耐药性监测[J].中国感染与化疗杂志,2012,12(3):174-179.ZHUO Chao,SU Dan-hong,NI Yu-xing,et al.CHINETsurveillance of antimicrobial resistance in Klebsiella spp.during 2010[J].Chinese Journal of Infection and Chemotherapy,2012,12(3):174-179.(in Chinese)