云南葡萄炭疽病菌遗传多样性分析
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摘要
本研究利用β-tub2,ef1-α和his4基因部分序列对60株采自云南省不同葡萄产区的葡萄炭疽病菌菌株进行基因谱系分析。结果表明:引起云南葡萄炭疽病的病原菌种类较为复杂,不仅包括胶孢炭疽菌复合种群中的Colletotrichum gloeosporioides,还有胶孢炭疽菌复合种群中的其他种或炭疽菌属其他种。来源于不同产地不同葡萄品种上的炭疽菌遗传多样性丰富,但分子水平的多样性与菌株的地理来源、寄主种类、形态特征及对红提致病力方面无明显相关性。
The genetic diversity of 60 Colletotrichum spp. isolates collected from main grape-growing areas in Yunnan was characterized with the molecular phylogenetic tree of β-tub2,ef1-α,and his4 partial gene sequence. The results indicated that grape anthracnose was caused not only by Colletotrichum gloeosporioides,but also by other species within the C. gloeosporioides complex,and other species in Colletotrichum. Further analysis demonstrated that there was rich genetic diversity level within the population isolated from different grape specificities coming from different areas in Yunnan. However,the genetic diversity of these isolates had no correlation with those characterizations including geographic origins,host specificities,colony or spore morphologies and pathogenicity to red globe grape.
The genetic diversity of 60 Colletotrichum spp. isolates collected from main grape-growing areas in Yunnan was characterized with the molecular phylogenetic tree of β-tub2,ef1-α,and his4 partial gene sequence. The results indicated that grape anthracnose was caused not only by Colletotrichum gloeosporioides,but also by other species within the C. gloeosporioides complex,and other species in Colletotrichum. Further analysis demonstrated that there was rich genetic diversity level within the population isolated from different grape specificities coming from different areas in Yunnan. However,the genetic diversity of these isolates had no correlation with those characterizations including geographic origins,host specificities,colony or spore morphologies and pathogenicity to red globe grape.
引文
[1]DAMM U,CANNON P F,CROUS P W.Colletotrichum:complex species or species complexes[J].Studies in Mycology,2012,73(1):iii.
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[3]GREER L A,HARPER J D I,SAVOCCHIA S,et al.Ripe rot of south-eastern Australian wine grapes is caused by two species of Colletotrichum:C.acutatum and C.gloeosporioides with differences in infection and fungicide sensitivity[J].Australian Journal of Grape and Wine Research,2011,17(2):123-128.
[4]SHIRAISHI M,KOIDE M,ITAMURA H,et al.Screening for resistance to ripe rot caused by Colletotrichum acutatum in grape germplasm[J].Vitis,2007,46(4):196-200.
[5]WHITELAW-WECKERT M A,CURTIN S J,HUANG R,et al.Phylogenetic relationships and pathogenicity of Colletotrichum acutatum isolates from grape in subtropical Australia[J].Plant Pathology,2007,56(3):448-463.
[6]HYDE K D,CAI Lei,CANNON P F,et al.Colletotrichum names in current use[J].Fungal Diversity,2009,39:147-182.
[7]SACCARDO P A.Sylloge fungorum omnium husque cognitorum[M].Sumptibus Auctoris,1913,22:1199.
[8]PENG Lijuan,SUN Tao,YANG Youlian,et al.Colletotrichum species on grape in Guizhou and Yunnan Provinces,China[J].Mycoscience,2013,54(1):29-41.
[9]邓维萍,杨敏,杜飞,等.云南葡萄产区葡萄炭疽病病原鉴定及致病力分析[J].植物保护学报,2013(1):61-67.
[10]WEIR B S,JOHNSTON P R,DAMM U.The Colletotrichum gloeosporioides species complex[J].Studies in Mycology,2012,73(1):115-180.
[11]CAI Lei,HYSW K D,TAYLOR P W J,et al.A polyphasic approach for studying Colletotrichum[J].Fungal Diversity,2009,39(1):183-204.
[12]HYDE K D,CHOMNUNTI P,CROUS P W.et al.A case for reinventory of Australia's plant pathogens[J].Persoonia,2010,25:50-60.
[13]SCHOCH C L,SEIFERT K A,HUHNDORF S,et al.Nuclear ribosomal internal transcribed spacer(ITS)region as a universal DNA barcode marker for Fungi[J].Proceedings of the National Academy of Sciences,2012,109(16):6241-6246.
[14]MILLS P R,SREENIVASAPRASAD S,BROWN A E.Detection and differentiation of Colletotrichum gloeosporioides isolates using PCR[J].Federation of European Microbiological Societies(FEMS)Microbiology Letters,1992,98(1):137-143.
[15]THON M R,ROYSE D J.Evidence for two independent lineagent of shiitake of the Americas(Lentinula boryana)based on r DNA and ofβ-tubulin genes sequences[J].Molecular Phylogenetics Evolution,1999,13(3):520-524.
[16]SHEN Qing,GEISER D M,ROYSE D J.Molecular phylogenetic analysis of Grifola frondosa(maitake)reveals a species partition separating eastern North American and Asian isolates[J].Mycologia,2002,94(3):472-482.
[17]THAN P P,SHIVAS R G,JEEWON R,et al.Epitypification and phylogeny of Colletotrichum acutatum J.H.Simmonds[J].Fungal Diversity,2008,28:97-108.
[18]YANG Y L,LIU Z Y,CAI L,et al.Colletotrichum anthracnose of Amaryllidaceae[J].Fungal Diversity,2009,39:123.
[19]WEIR B S,JOHNSTON P R.Characterization and neotypification of Gloeosporium kaki Hori as Colletotrichum horii nom.nov.[J].Mycotaxon,2010,111(1):209-219.
[20]SU Yuanying,NOIREUNG P,LIU Fang,et al.Epitypification of Colletotrichum musae,the causative agent of banana anthracnose[J].Mycoscience,2011,52(6):376-382.
[21]ROJAS E I,REHNER S A,SAMUELS G J,et al.Colletotrichum gloeosporioides sl associated with Theobroma cacao and other plants in Panamá:multilocus phylogenies distinguish host-associated pathogens from asymptomatic endophytes[J].Mycologia,2010,102(6):1318-1338.
[22]PHOULIVONG S,CAI Lei,PARINN N,et al.A new species of Colletotrichum from Cordyline fruticosa and Eugenia javanica causing anthracnose disease[J].Mycotaxon,2011,114(1):247-257.
[23]WIKEE S,CAI Lei,PAIRIN N,et al.Colletotrichum species from Jasmine(Jasminum sambac)[J].Fungal Diversity,2011,46(1):171-182.
[24]MCGARVEY P,KAPER J M.A simple and rapid method for screening transgenic plants using the PCR[J].Biotechniques,1991,11(4):428-432.
[25]GLASS N,DONALDSON G.Development of primer sets designed for use with the PCR to amplify conserved genes from Flamentous ascomycetes[J].Applied and Environmental Microbiology,1995,61(4):1323-1330.
[26]O'DONNELL K,KISTLER H C,CIGELNIK E,et al.Multiple evolutionary origin of the fungus causing Panama disease of banana;concordant evidence from nuclear and mitochondrial gene genealogies[J].Proceedings of the National Academy of Sciences of USA,1998,95(5):2044-2049.
[27]VON ARX J A.A revision of the fungi classified as genus Gloeosporium[J].Bibliotheca Mycologica,1970,24:201-203.
[28]刘开启,郭烁.果树胶孢炭疽菌的RAPD分析[J].仲恺农业技术学院学报,2002,15(4):1-7.
[29]FITZPATRICK D A,LOGUE M E,STAJICH J E,et al.A fungal phylogeny based on 42 complete genomes derived from supertree and combined gene analysis[J].BMC Evolutionary Biology,2006,6(1):99.
[30]AGUILETA G,MARTHEY S,CHIAPELLO H,et al.Assessing the Performance of Single-Copy Genes for Recovering Robust Phylogenies[J].Systematic Biology,2008,57(4):613-627.
[31]WEIR B S,JOHNSTON P R.Towards a secondary fungal barcode for Colletotrichum(Sordariomycetes,Ascomycota)[C]//Forth international barcode of life conference,Adelaide Australia,2011.
[32]TALHINHAS P,SREENIVASAPRASAD S,NEVES-MARTINS J,et al.Genetic and morphological characterization of Colletotrichum acutatum causing anthracnose of lupins[J].Phytopathology,2002,92(9),986-996.
[33]李利霞,赵奎华,刘长远.葡萄炭疽病菌SRAP遗传多样性分析[J].中国农学通报,2012,28(12):230-235.
[34]徐红梅,管兰华,韩正敏.用RAPD标记研究中国木本植物胶孢炭疽菌的群体分化[J].东北林业大学学报,2004,32(6):55-57.
[35]LOTTER H C,BERGER D K.Anthracnose of lupins in South Africa is caused by Colletotrichum lupini var.setosum[J].Australasian Plant Pathology,2005,34(3):385-392.
[36]杨致年,冷怀琼.柑橘上胶孢炭疽菌生理分化的研究[J].四川农业大学学报,1991,9(2):215-235.
[37]陈国庆.中国柑橘炭疽病病原种类及种群遗传多样性研究[D].杭州:浙江大学,2010:43-55.
[38]尚晶晶.中国葡萄炭疽病病原菌的鉴定及种群分化的研究[D].呼和浩特:内蒙古农业大学,2010:47-52.
[2]SUNG K H,WAN G K,HAE K Y,et al.Morphological variations,genetic diversity and pathogenicity of Colletotrichum species causing grape ripe rot in Korea[J].Plant Pathology,2008,24(3):269-278.
[3]GREER L A,HARPER J D I,SAVOCCHIA S,et al.Ripe rot of south-eastern Australian wine grapes is caused by two species of Colletotrichum:C.acutatum and C.gloeosporioides with differences in infection and fungicide sensitivity[J].Australian Journal of Grape and Wine Research,2011,17(2):123-128.
[4]SHIRAISHI M,KOIDE M,ITAMURA H,et al.Screening for resistance to ripe rot caused by Colletotrichum acutatum in grape germplasm[J].Vitis,2007,46(4):196-200.
[5]WHITELAW-WECKERT M A,CURTIN S J,HUANG R,et al.Phylogenetic relationships and pathogenicity of Colletotrichum acutatum isolates from grape in subtropical Australia[J].Plant Pathology,2007,56(3):448-463.
[6]HYDE K D,CAI Lei,CANNON P F,et al.Colletotrichum names in current use[J].Fungal Diversity,2009,39:147-182.
[7]SACCARDO P A.Sylloge fungorum omnium husque cognitorum[M].Sumptibus Auctoris,1913,22:1199.
[8]PENG Lijuan,SUN Tao,YANG Youlian,et al.Colletotrichum species on grape in Guizhou and Yunnan Provinces,China[J].Mycoscience,2013,54(1):29-41.
[9]邓维萍,杨敏,杜飞,等.云南葡萄产区葡萄炭疽病病原鉴定及致病力分析[J].植物保护学报,2013(1):61-67.
[10]WEIR B S,JOHNSTON P R,DAMM U.The Colletotrichum gloeosporioides species complex[J].Studies in Mycology,2012,73(1):115-180.
[11]CAI Lei,HYSW K D,TAYLOR P W J,et al.A polyphasic approach for studying Colletotrichum[J].Fungal Diversity,2009,39(1):183-204.
[12]HYDE K D,CHOMNUNTI P,CROUS P W.et al.A case for reinventory of Australia's plant pathogens[J].Persoonia,2010,25:50-60.
[13]SCHOCH C L,SEIFERT K A,HUHNDORF S,et al.Nuclear ribosomal internal transcribed spacer(ITS)region as a universal DNA barcode marker for Fungi[J].Proceedings of the National Academy of Sciences,2012,109(16):6241-6246.
[14]MILLS P R,SREENIVASAPRASAD S,BROWN A E.Detection and differentiation of Colletotrichum gloeosporioides isolates using PCR[J].Federation of European Microbiological Societies(FEMS)Microbiology Letters,1992,98(1):137-143.
[15]THON M R,ROYSE D J.Evidence for two independent lineagent of shiitake of the Americas(Lentinula boryana)based on r DNA and ofβ-tubulin genes sequences[J].Molecular Phylogenetics Evolution,1999,13(3):520-524.
[16]SHEN Qing,GEISER D M,ROYSE D J.Molecular phylogenetic analysis of Grifola frondosa(maitake)reveals a species partition separating eastern North American and Asian isolates[J].Mycologia,2002,94(3):472-482.
[17]THAN P P,SHIVAS R G,JEEWON R,et al.Epitypification and phylogeny of Colletotrichum acutatum J.H.Simmonds[J].Fungal Diversity,2008,28:97-108.
[18]YANG Y L,LIU Z Y,CAI L,et al.Colletotrichum anthracnose of Amaryllidaceae[J].Fungal Diversity,2009,39:123.
[19]WEIR B S,JOHNSTON P R.Characterization and neotypification of Gloeosporium kaki Hori as Colletotrichum horii nom.nov.[J].Mycotaxon,2010,111(1):209-219.
[20]SU Yuanying,NOIREUNG P,LIU Fang,et al.Epitypification of Colletotrichum musae,the causative agent of banana anthracnose[J].Mycoscience,2011,52(6):376-382.
[21]ROJAS E I,REHNER S A,SAMUELS G J,et al.Colletotrichum gloeosporioides sl associated with Theobroma cacao and other plants in Panamá:multilocus phylogenies distinguish host-associated pathogens from asymptomatic endophytes[J].Mycologia,2010,102(6):1318-1338.
[22]PHOULIVONG S,CAI Lei,PARINN N,et al.A new species of Colletotrichum from Cordyline fruticosa and Eugenia javanica causing anthracnose disease[J].Mycotaxon,2011,114(1):247-257.
[23]WIKEE S,CAI Lei,PAIRIN N,et al.Colletotrichum species from Jasmine(Jasminum sambac)[J].Fungal Diversity,2011,46(1):171-182.
[24]MCGARVEY P,KAPER J M.A simple and rapid method for screening transgenic plants using the PCR[J].Biotechniques,1991,11(4):428-432.
[25]GLASS N,DONALDSON G.Development of primer sets designed for use with the PCR to amplify conserved genes from Flamentous ascomycetes[J].Applied and Environmental Microbiology,1995,61(4):1323-1330.
[26]O'DONNELL K,KISTLER H C,CIGELNIK E,et al.Multiple evolutionary origin of the fungus causing Panama disease of banana;concordant evidence from nuclear and mitochondrial gene genealogies[J].Proceedings of the National Academy of Sciences of USA,1998,95(5):2044-2049.
[27]VON ARX J A.A revision of the fungi classified as genus Gloeosporium[J].Bibliotheca Mycologica,1970,24:201-203.
[28]刘开启,郭烁.果树胶孢炭疽菌的RAPD分析[J].仲恺农业技术学院学报,2002,15(4):1-7.
[29]FITZPATRICK D A,LOGUE M E,STAJICH J E,et al.A fungal phylogeny based on 42 complete genomes derived from supertree and combined gene analysis[J].BMC Evolutionary Biology,2006,6(1):99.
[30]AGUILETA G,MARTHEY S,CHIAPELLO H,et al.Assessing the Performance of Single-Copy Genes for Recovering Robust Phylogenies[J].Systematic Biology,2008,57(4):613-627.
[31]WEIR B S,JOHNSTON P R.Towards a secondary fungal barcode for Colletotrichum(Sordariomycetes,Ascomycota)[C]//Forth international barcode of life conference,Adelaide Australia,2011.
[32]TALHINHAS P,SREENIVASAPRASAD S,NEVES-MARTINS J,et al.Genetic and morphological characterization of Colletotrichum acutatum causing anthracnose of lupins[J].Phytopathology,2002,92(9),986-996.
[33]李利霞,赵奎华,刘长远.葡萄炭疽病菌SRAP遗传多样性分析[J].中国农学通报,2012,28(12):230-235.
[34]徐红梅,管兰华,韩正敏.用RAPD标记研究中国木本植物胶孢炭疽菌的群体分化[J].东北林业大学学报,2004,32(6):55-57.
[35]LOTTER H C,BERGER D K.Anthracnose of lupins in South Africa is caused by Colletotrichum lupini var.setosum[J].Australasian Plant Pathology,2005,34(3):385-392.
[36]杨致年,冷怀琼.柑橘上胶孢炭疽菌生理分化的研究[J].四川农业大学学报,1991,9(2):215-235.
[37]陈国庆.中国柑橘炭疽病病原种类及种群遗传多样性研究[D].杭州:浙江大学,2010:43-55.
[38]尚晶晶.中国葡萄炭疽病病原菌的鉴定及种群分化的研究[D].呼和浩特:内蒙古农业大学,2010:47-52.