基于界面活化的脂肪酶固定化方法研究进展
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摘要
界面活化作用可以显著提高脂肪酶的催化活性,对脂肪酶在所有领域的应用均有重要影响。因此,在脂肪酶固定化方法的设计过程中,应该要考虑到界面活化作用的重要性。本文综述了国内外基于界面活化的脂肪酶固定化方法研究进展,并结合绿色高效型生物催化剂的发展要求对新型脂肪酶固定化方法的研究方向及发展前景提出了展望。
Since interfacial activation can cause a dramatic increase in catalytic activity,it is of profound importance for all applications of lipases,and should always be considered when developing lipase immobilization methods.In this thesis,the recent developments of potential lipase immobilization based on interfacial activation were discussed,and the outlook of lipase immobilization was also prospected.
Since interfacial activation can cause a dramatic increase in catalytic activity,it is of profound importance for all applications of lipases,and should always be considered when developing lipase immobilization methods.In this thesis,the recent developments of potential lipase immobilization based on interfacial activation were discussed,and the outlook of lipase immobilization was also prospected.
引文
[1]DiCosimo R,McAuliffe J,Poulose,AJ,et al.Industrial use of immobilized enzymes[J].Chem Soc Rev,2013,42,6 437-6 474.
[2]Popp S,Packschies L,Radzwill N,et al.Structural dynamics of the DnaK-peptide complex[J].J Mol Biol,2005,347(5):1 039-1 052.
[3]Gutsche I,Essen LO,Baumeister W.Group II chaperonins:new TriC(k)s and turns of a protein folding machine[J].J Mol Biol,1999,293(2):295-312.
[4]Rehm S,Trodler P,Pleiss J.Solvent-induced lid opening in lipases:a molecular dynamics study[J].Protein Sci,2010,19:2 122-2 130.
[5]Fernandez LG,Cabrera Z,Godoy C,et al.Interfacially activated lipases against hydrophobic supports:effect of the support nature on the biocatalytic properties[J].Process Biochem,2008,43(10):1 061-1 067.
[6]Jones GA and Wu Y.Effect of limited proteolysis on phospholipase C-1kinetics[J].Arch Biochem Biophys,2000,375(2):229-239.
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[8]Verger R.Interfacial activation of lipases:facts and artifacts[J].Trends Biotechnol,1997,15:32-38.
[9]Berg O G,Cajal Y,Butterfoss GL,et al.Interfacial activation of triglyceride lipase from Thermomyces(Humicola)lanuginosa:kinetic parameters and a basis for control of the lid[J].Biochemistry,1998,37:6 615-6 627.
[10]Maraite A,Hoyos P,Carballeira JD,et al.Lipase from Pseudomonas stutzeri:purification,homology modelling and rational explanation of the substrate binding mode[J].J Mol Catal B:Enzym,2013,87:88-98.
[11]Hanefeld U,Gardossi L,Magner E.Understanding enzyme immobilisation[J].Chem Soc Rev,2009,38:453-468.
[12]史文婷,黄岩,汪秀丽.碳酸钙固定化猪胰脂肪酶的稳定性及催化PDO开环聚合研究[J].化学研究与应用,2014,7:1 126-1 129.
[13]Zheng MM,Lu Y,Dong L,et al.Immobilization of candida rugosa lipase on hydrophobic/strong cationexchange functional silica particles for biocatalytic synthesis of phytosterol esters[J].Bioresour Technol,2012,115:141-146.
[14]Bastida A,Sabuquillo P,Armisen P,et al.A single step purification,immobilization,and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports[J].Biotechnol Bioeng,1998,58:486-493.
[15]Wan Y Zhao DY.On the controllable soft-templating approach to mesoporous silicates[J].Chem Rev,2007,107:2 821-2 860.
[16]Jin QR,Jia GQ,Zhang YM,et al.Hydrophobic surface induced activation of Pseudomonas cepacia lipase immobilized into mesoporous silica[J].Langmuir,2011,27:12 016-12 024.
[17]Lee DG,Ponvel KM,Kim M,et al.Immobilization of lipase on hydrophobic nano-sized magnetite particles[J].J Mol Catal B:Enzym,2009,57:62-66.
[18]Filice M,Marciello M,Betancor L,et al.Hydrolysis of fish oil by hyperactivated Rhizomucor miehei lipase immobilized by multipoint anion exchange[J].Biotechnol Prog,2011,27:961-968.
[19]Tacias-Pascacio VG,Virgen-Ortíz JJ,Jiménez-Pérez M,et al.Evaluation of different lipase biocatalysts in the production of biodiesel from used cooking oil:critical role of the immobilization support[J].Fuel,2017,200:1-10
[20]Laszlo JA,Jackson M,Blanco RM.Active-site titration analysis of surface influences on immobilized Candida antarctica lipase B activity[J].J Mol Catal B:Enzym,2011,69:60-65.
[21]Weiser D,Boros Z,Hornyanszky G,et al.Disubstituted dialkoxysilane precursors in binary and ternary sol-gel systems for lipase immobilization[J].Process Biochem,2012,47:428-434.
[22]Dech S,Wruk V,Fik CP,et al.Amphiphilic polymer conetworks derived from aqueous solutions for biocatalysis in organic solvents[J].Polymer,2012,53:701-707.
[23]Gawlitza K,Wu CZ,Georgieva R,et al.Immobilization of lipase B within micron-sized poly-N-isopropylacrylamide hydrogel particles by solvent exchange[J].Phys Chem Chem Phys,2012,14:9 594-9 600.
[24]Khalaf N,Govardhan CP,Lalonde JL,et al.Cross-linked enzyme crystals as highly active catalysts in organic solvents[J].J Am Chem Soc,1996,118:5 494-5 495.
[25]Sheldon,R.A.Characteristic features and biotechnological applications of cross-linked enzyme aggregates(CLEAs)[J].Appl Microbiol Biotechnol,2011,92:467-477.
[26]Zhang WW,Yang XL,Jia JQ,et al.Surfactant-activated magnetic cross-linked enzyme aggregates(magnetic CLEAs)of Thermomyces lanugunosus lipase for biodiesel production[J].J Mol Catal B:Enzym,2015,115:83-89
[27]Zoumpanioti M,Stamatis H,Xenakis A.Microemulsion-based organogels as matrices for lipase immobilization[J].Biotechnology Advances,2010,28:395-406.
[28]Nagayama K,Imai M.Enhanced activity of Mucor javanicus lipase in polyoxyethylene sorbitan trioleate containing microemulsion-based organogels[J].J Mol Catal B:Enzym,2005,34:44-50.
[29]Zoumpanioti M,Parmaklis P,Dominguez de Maria P,et al.Esterification reactions catalyzed by lipases immobilized in organogels.Effect of temperature and substrate diffusion[J].Biotechnol Lett,2008,30:1627-1 631.
[30]Itabaiana I,Gon?alves KM,Zoumpanioti M,et al.Microemulsion-based organogels as an efficient support for lipase-catalyzed reactions under continuous-flow conditions[J].Org Process Res Dev,2014,18:1 372-1376.
[31]Zhang WW,Wang N,Zhou YJ,et al.Enhancement of activity and stability of lipase by microemulsion-based organogels(MBGs)immobilization and application for synthesis of arylethyl acetate[J].J Mol Catal B:Enzym,2012,78:65-71.
[32]Zhang WW,Wang N,Zhang L,et al.Effects of additives on lipase immobilization in microemulsion-based organogels[J].Appl Biochem Biotechnol,2014,172:3128-3 140
[33]Zhang WW,Jia JQ,Wang N,et al.Improved activity of lipase immobilized in microemulsion-based organogels for(R,S)-ketoprofen ester resolution:long-term stability and reusability[J].Biotechnology Reports,2015,7:1-8
[2]Popp S,Packschies L,Radzwill N,et al.Structural dynamics of the DnaK-peptide complex[J].J Mol Biol,2005,347(5):1 039-1 052.
[3]Gutsche I,Essen LO,Baumeister W.Group II chaperonins:new TriC(k)s and turns of a protein folding machine[J].J Mol Biol,1999,293(2):295-312.
[4]Rehm S,Trodler P,Pleiss J.Solvent-induced lid opening in lipases:a molecular dynamics study[J].Protein Sci,2010,19:2 122-2 130.
[5]Fernandez LG,Cabrera Z,Godoy C,et al.Interfacially activated lipases against hydrophobic supports:effect of the support nature on the biocatalytic properties[J].Process Biochem,2008,43(10):1 061-1 067.
[6]Jones GA and Wu Y.Effect of limited proteolysis on phospholipase C-1kinetics[J].Arch Biochem Biophys,2000,375(2):229-239.
[7]Miled N,Bussetta C,De caro A,et al.Importance of the lid and cap domains for the catalytic activity of gastric lipases[J].Comp Biochem Phys B,2003,136(1):131-138.
[8]Verger R.Interfacial activation of lipases:facts and artifacts[J].Trends Biotechnol,1997,15:32-38.
[9]Berg O G,Cajal Y,Butterfoss GL,et al.Interfacial activation of triglyceride lipase from Thermomyces(Humicola)lanuginosa:kinetic parameters and a basis for control of the lid[J].Biochemistry,1998,37:6 615-6 627.
[10]Maraite A,Hoyos P,Carballeira JD,et al.Lipase from Pseudomonas stutzeri:purification,homology modelling and rational explanation of the substrate binding mode[J].J Mol Catal B:Enzym,2013,87:88-98.
[11]Hanefeld U,Gardossi L,Magner E.Understanding enzyme immobilisation[J].Chem Soc Rev,2009,38:453-468.
[12]史文婷,黄岩,汪秀丽.碳酸钙固定化猪胰脂肪酶的稳定性及催化PDO开环聚合研究[J].化学研究与应用,2014,7:1 126-1 129.
[13]Zheng MM,Lu Y,Dong L,et al.Immobilization of candida rugosa lipase on hydrophobic/strong cationexchange functional silica particles for biocatalytic synthesis of phytosterol esters[J].Bioresour Technol,2012,115:141-146.
[14]Bastida A,Sabuquillo P,Armisen P,et al.A single step purification,immobilization,and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports[J].Biotechnol Bioeng,1998,58:486-493.
[15]Wan Y Zhao DY.On the controllable soft-templating approach to mesoporous silicates[J].Chem Rev,2007,107:2 821-2 860.
[16]Jin QR,Jia GQ,Zhang YM,et al.Hydrophobic surface induced activation of Pseudomonas cepacia lipase immobilized into mesoporous silica[J].Langmuir,2011,27:12 016-12 024.
[17]Lee DG,Ponvel KM,Kim M,et al.Immobilization of lipase on hydrophobic nano-sized magnetite particles[J].J Mol Catal B:Enzym,2009,57:62-66.
[18]Filice M,Marciello M,Betancor L,et al.Hydrolysis of fish oil by hyperactivated Rhizomucor miehei lipase immobilized by multipoint anion exchange[J].Biotechnol Prog,2011,27:961-968.
[19]Tacias-Pascacio VG,Virgen-Ortíz JJ,Jiménez-Pérez M,et al.Evaluation of different lipase biocatalysts in the production of biodiesel from used cooking oil:critical role of the immobilization support[J].Fuel,2017,200:1-10
[20]Laszlo JA,Jackson M,Blanco RM.Active-site titration analysis of surface influences on immobilized Candida antarctica lipase B activity[J].J Mol Catal B:Enzym,2011,69:60-65.
[21]Weiser D,Boros Z,Hornyanszky G,et al.Disubstituted dialkoxysilane precursors in binary and ternary sol-gel systems for lipase immobilization[J].Process Biochem,2012,47:428-434.
[22]Dech S,Wruk V,Fik CP,et al.Amphiphilic polymer conetworks derived from aqueous solutions for biocatalysis in organic solvents[J].Polymer,2012,53:701-707.
[23]Gawlitza K,Wu CZ,Georgieva R,et al.Immobilization of lipase B within micron-sized poly-N-isopropylacrylamide hydrogel particles by solvent exchange[J].Phys Chem Chem Phys,2012,14:9 594-9 600.
[24]Khalaf N,Govardhan CP,Lalonde JL,et al.Cross-linked enzyme crystals as highly active catalysts in organic solvents[J].J Am Chem Soc,1996,118:5 494-5 495.
[25]Sheldon,R.A.Characteristic features and biotechnological applications of cross-linked enzyme aggregates(CLEAs)[J].Appl Microbiol Biotechnol,2011,92:467-477.
[26]Zhang WW,Yang XL,Jia JQ,et al.Surfactant-activated magnetic cross-linked enzyme aggregates(magnetic CLEAs)of Thermomyces lanugunosus lipase for biodiesel production[J].J Mol Catal B:Enzym,2015,115:83-89
[27]Zoumpanioti M,Stamatis H,Xenakis A.Microemulsion-based organogels as matrices for lipase immobilization[J].Biotechnology Advances,2010,28:395-406.
[28]Nagayama K,Imai M.Enhanced activity of Mucor javanicus lipase in polyoxyethylene sorbitan trioleate containing microemulsion-based organogels[J].J Mol Catal B:Enzym,2005,34:44-50.
[29]Zoumpanioti M,Parmaklis P,Dominguez de Maria P,et al.Esterification reactions catalyzed by lipases immobilized in organogels.Effect of temperature and substrate diffusion[J].Biotechnol Lett,2008,30:1627-1 631.
[30]Itabaiana I,Gon?alves KM,Zoumpanioti M,et al.Microemulsion-based organogels as an efficient support for lipase-catalyzed reactions under continuous-flow conditions[J].Org Process Res Dev,2014,18:1 372-1376.
[31]Zhang WW,Wang N,Zhou YJ,et al.Enhancement of activity and stability of lipase by microemulsion-based organogels(MBGs)immobilization and application for synthesis of arylethyl acetate[J].J Mol Catal B:Enzym,2012,78:65-71.
[32]Zhang WW,Wang N,Zhang L,et al.Effects of additives on lipase immobilization in microemulsion-based organogels[J].Appl Biochem Biotechnol,2014,172:3128-3 140
[33]Zhang WW,Jia JQ,Wang N,et al.Improved activity of lipase immobilized in microemulsion-based organogels for(R,S)-ketoprofen ester resolution:long-term stability and reusability[J].Biotechnology Reports,2015,7:1-8