AG1478抑制剂对白鲫体色发育的影响
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摘要
鱼类的体色由基本色素细胞相互配合而成,色素细胞主要有4种类型:黑色素细胞、红色素细胞、黄色素细胞和虹彩细胞。AG1478是针对酪氨酸激酶(Tyr)的一种新型抑制剂。Tyr是黑色素生成过程中的关键酶,在黑色素细胞中呈特异性表达。本文主要阐述了白鲫四种色素细胞以及两种不同形态黑色素细胞的超微结构观察,探讨了AG1478抑制剂对白鲫早期体色发育的影响,实验结果表明抑制剂对白鲫早期体色发育没有明显影响。
There are four types of pigment cells in fish,including melanocytes,erythrocytes,xanthophores,and iridocytes. AG1478 is a novel inhibitor against Tyrosinase( Tyr). Tyr is a key enzyme in melanin production and it is specifically expressed in melanocytes. In the paper,we studied the ultrastructural observation of four pigment cells and two different shapes of melanocytes in white crucian. We also investigated the effect of AG1478 inhibitors on the early body color development of white crucian. Our data indicate that the AG1478 inhibitor had few effect on the early body color development of white crucian.
There are four types of pigment cells in fish,including melanocytes,erythrocytes,xanthophores,and iridocytes. AG1478 is a novel inhibitor against Tyrosinase( Tyr). Tyr is a key enzyme in melanin production and it is specifically expressed in melanocytes. In the paper,we studied the ultrastructural observation of four pigment cells and two different shapes of melanocytes in white crucian. We also investigated the effect of AG1478 inhibitors on the early body color development of white crucian. Our data indicate that the AG1478 inhibitor had few effect on the early body color development of white crucian.
引文
[1]付小全.同体色萍乡红鲫皮肤组织学、黑色素浓集激素受体及细胞色素b基因的研究[D].南昌:南昌大学,2008.FU Xiaoquan.The study on skin histology,melanin-concentrating hormone receptor and cytochrome b gene in different bodycolor of Carssius auratus Vra.Pingxiangnensis[D].Nanchang:Nanchang University,2008.
[2]陈桢.金鱼家化史与品种形成的因素[J].动物学报,1954,6(2):89-116.CHEN Zhen.A history of the domestication and the factors of the varietal formation of the common goldfish,Carassius auratus[J].Acta Zoologica Sinica,1954,6(2):89-116.
[3]FUJII R.The regulation of motile activity in fish ehromatophore[J].Pigment Cell Research,2000,13(5):300-319.
[4]李小兵,郑曙明,吴青.曼龙鱼色素细胞的显微观察[J].四川动物,2012,31(4):538-541.LI Xiaobing,ZHENG Shuming,WU Qing.Microscopic observation on chromatophores of trichogaster trichopterus[J].Sichuan Journal of Zoology,2012,31(4):538-541.
[5]黄永政.鱼类体色研究进展[J].水产学杂志,2008,21(1):89-94.HUANG Yongzheng.Advances in the study of fish body color[J].Chinese Journal of Fisheries,2008,21(1):89-94.
[6]OSHIMA N,NAKAMARU N,ARAKI S,et al.Comparative analyses of the pigment-aggregating and dispersing actions of MCH on fish chromatophores[J].Comparative Biehemistry and PhysioIogy Part C,2001,129(2):75-84.
[7]KELSH R N.Genetics and evolution of pigment patterns in fish[J].Pigment Cell&Melanoma Research,2004,17(4):326-336.
[8]LIU J H,ZHANG Y Q,GUI S Y,et al.Observation and regression models on body colour inheritance and development in crucian carp and carp[J].Aquaculture International,2016,24(4):1191-1199.
[9]LIU J H,WEN S,LUO C,et al.Involvement of the MITFAgene in the development of pigment cell in Japanese ornamental(Koi)carp(Cyprinus carpio L)[J].Genetics and Molecular Research(GMR),2015,14(1):2775-2784.
[10]ZHANG Y Q,LIU J H,PENG L Y,et al.Comparative transcriptome analysis of molecular mechanism underlying gray-tored body color formation in red crucian carp(Carassius auratus,red var.)[J].Fish Physiology and Biochemistry,2017,43(5):1387-1398.
[11]ZHANG Y Q,LIU J H,F W,et al.Comparative transcriptome and DNA methylation analyses of the molecular mechanisms underlying skin color variations in Crucian carp(Carassius carassius L)[J].BMC Genetics,2017,18(1):95-107.
[12]POLIER S,SAMANT R S,CLARKE P A,et al.ATP competitive inhibitors block protein kinase recruitment to the Hsp90Cdc37 system[J].Nature Chemical Biology,2013,9(5):307-312
[13]YU Y,FAN S M,YE Y C,et al.The tyrphostin AG1478 augments oridonin induced A431 cell apoptosis by blockage of JNKMAPK and enhancement of oxidative stress[J].Free Radic Research,2012,46(11):1393-1405.
[14]马聪玲,殷一萍,毛婷婷.EGFR抑制剂AG1478对宫颈癌HeLa细胞凋亡的影响[J].实用医学杂志,2017,33(2):202-205.MA Congling,YIN Yiping,MAO Tingting.Effect of epidermal growth factor receptor inhibitor AG1478 induces cell death in He La cells[J].The Journal of Practical Medicine,2017,33(2):202-205.
[15]WITKOP C J,WHITE J G,NANCE W E,et al.Mutations in the melanin pigment system in man resulting in features of oculocutaneous albinism[J].Appleton Century Crofts,1972,22(1):359-377.
[16]YOKOYAMA T,SILVERSIDES D,WAYMIRE K,et al.Conserved cysteine to serine mutation in tyrosinase is responsible for the classical albino mutation in laboratory mice[J].Nucleic Acids Research,1990,18(24):7293-7298.
[17]KOGA A,HORI H.Albinism due to transposable element insertion in fish[J].Pigment Cell Research,1997,10(6):377-381.
[18]KOGA A,WAKAMATSU Y,KUROSAWA J,et al.Oculocutaneous albinism in the i6 mutant of the medaka fish is associated with a deletion in the Tyrosinase gene[J].Pigment Cell Research,1999,12(4):252-258.
[19]KOGA A,INAGAKi H,BESSHO Y,et al.Insertion of a novel transposable element in the tyrosinase gene is responsible for an albino mutation in the medaka fish,Oryzias latipes[J].Molecular and General Genetics,1995,249(4):400-405.
[20]张艳苹.红鳍笛鲷皮肤转录组分析及体色相关基因克隆、表达与进化研究[D].长沙:湖南师范大学博士论文,2016.ZHANG Yanping.Study on skin transcriptome of Crimson snapper(Lutjanus erythropterus),and their pigment genes related cloning expression and evolution analysis[D].Changsha:Doctoral Dissertation of Hunan Normal University,2016.
[21]文胜.锦鲤体色形成观察及mitf基因在锦鲤体色发育中的表达分析[D].长沙:湖南师范大学硕士论文,2014.WEN Sheng.Observation of koi body color formation and analysis of the expression of mitf gene in koi body color development[D].Changsha:Master’s Thesis of Hunan Normal University,2014.
[22]于道德,刘洪军,关健,等.黑棘鲷早期色素细胞发育与体色变化[J].渔业科学进展,2012,33(5):1-7.YU Daode,LIU Hongjun,GUAN Jian,et al.Early ontogeny of chromatophores and body color changes of Acanthopagrus schlegelii[J].Progress in Fishery Sciences,2012,33(5):1-7.
[23]YOUKO I.The erythrophore in the larval and adult dorsal skin of the brown frog,Rana ornatiuentris:its differentiation,migration,and pigmentary organelle formation[J].Pigment Cell Research,1998,11:345-354.
[24]HULTMAN KA,BLLDI E H,TEASLEY D C,et al.Defects in ErbB-Dependent establishment of adult melanocyte stem cells reveal independent origins for embryonic and regeneration melanocytes[J].Plos Genetics,2009,5(7):e1000544.
[2]陈桢.金鱼家化史与品种形成的因素[J].动物学报,1954,6(2):89-116.CHEN Zhen.A history of the domestication and the factors of the varietal formation of the common goldfish,Carassius auratus[J].Acta Zoologica Sinica,1954,6(2):89-116.
[3]FUJII R.The regulation of motile activity in fish ehromatophore[J].Pigment Cell Research,2000,13(5):300-319.
[4]李小兵,郑曙明,吴青.曼龙鱼色素细胞的显微观察[J].四川动物,2012,31(4):538-541.LI Xiaobing,ZHENG Shuming,WU Qing.Microscopic observation on chromatophores of trichogaster trichopterus[J].Sichuan Journal of Zoology,2012,31(4):538-541.
[5]黄永政.鱼类体色研究进展[J].水产学杂志,2008,21(1):89-94.HUANG Yongzheng.Advances in the study of fish body color[J].Chinese Journal of Fisheries,2008,21(1):89-94.
[6]OSHIMA N,NAKAMARU N,ARAKI S,et al.Comparative analyses of the pigment-aggregating and dispersing actions of MCH on fish chromatophores[J].Comparative Biehemistry and PhysioIogy Part C,2001,129(2):75-84.
[7]KELSH R N.Genetics and evolution of pigment patterns in fish[J].Pigment Cell&Melanoma Research,2004,17(4):326-336.
[8]LIU J H,ZHANG Y Q,GUI S Y,et al.Observation and regression models on body colour inheritance and development in crucian carp and carp[J].Aquaculture International,2016,24(4):1191-1199.
[9]LIU J H,WEN S,LUO C,et al.Involvement of the MITFAgene in the development of pigment cell in Japanese ornamental(Koi)carp(Cyprinus carpio L)[J].Genetics and Molecular Research(GMR),2015,14(1):2775-2784.
[10]ZHANG Y Q,LIU J H,PENG L Y,et al.Comparative transcriptome analysis of molecular mechanism underlying gray-tored body color formation in red crucian carp(Carassius auratus,red var.)[J].Fish Physiology and Biochemistry,2017,43(5):1387-1398.
[11]ZHANG Y Q,LIU J H,F W,et al.Comparative transcriptome and DNA methylation analyses of the molecular mechanisms underlying skin color variations in Crucian carp(Carassius carassius L)[J].BMC Genetics,2017,18(1):95-107.
[12]POLIER S,SAMANT R S,CLARKE P A,et al.ATP competitive inhibitors block protein kinase recruitment to the Hsp90Cdc37 system[J].Nature Chemical Biology,2013,9(5):307-312
[13]YU Y,FAN S M,YE Y C,et al.The tyrphostin AG1478 augments oridonin induced A431 cell apoptosis by blockage of JNKMAPK and enhancement of oxidative stress[J].Free Radic Research,2012,46(11):1393-1405.
[14]马聪玲,殷一萍,毛婷婷.EGFR抑制剂AG1478对宫颈癌HeLa细胞凋亡的影响[J].实用医学杂志,2017,33(2):202-205.MA Congling,YIN Yiping,MAO Tingting.Effect of epidermal growth factor receptor inhibitor AG1478 induces cell death in He La cells[J].The Journal of Practical Medicine,2017,33(2):202-205.
[15]WITKOP C J,WHITE J G,NANCE W E,et al.Mutations in the melanin pigment system in man resulting in features of oculocutaneous albinism[J].Appleton Century Crofts,1972,22(1):359-377.
[16]YOKOYAMA T,SILVERSIDES D,WAYMIRE K,et al.Conserved cysteine to serine mutation in tyrosinase is responsible for the classical albino mutation in laboratory mice[J].Nucleic Acids Research,1990,18(24):7293-7298.
[17]KOGA A,HORI H.Albinism due to transposable element insertion in fish[J].Pigment Cell Research,1997,10(6):377-381.
[18]KOGA A,WAKAMATSU Y,KUROSAWA J,et al.Oculocutaneous albinism in the i6 mutant of the medaka fish is associated with a deletion in the Tyrosinase gene[J].Pigment Cell Research,1999,12(4):252-258.
[19]KOGA A,INAGAKi H,BESSHO Y,et al.Insertion of a novel transposable element in the tyrosinase gene is responsible for an albino mutation in the medaka fish,Oryzias latipes[J].Molecular and General Genetics,1995,249(4):400-405.
[20]张艳苹.红鳍笛鲷皮肤转录组分析及体色相关基因克隆、表达与进化研究[D].长沙:湖南师范大学博士论文,2016.ZHANG Yanping.Study on skin transcriptome of Crimson snapper(Lutjanus erythropterus),and their pigment genes related cloning expression and evolution analysis[D].Changsha:Doctoral Dissertation of Hunan Normal University,2016.
[21]文胜.锦鲤体色形成观察及mitf基因在锦鲤体色发育中的表达分析[D].长沙:湖南师范大学硕士论文,2014.WEN Sheng.Observation of koi body color formation and analysis of the expression of mitf gene in koi body color development[D].Changsha:Master’s Thesis of Hunan Normal University,2014.
[22]于道德,刘洪军,关健,等.黑棘鲷早期色素细胞发育与体色变化[J].渔业科学进展,2012,33(5):1-7.YU Daode,LIU Hongjun,GUAN Jian,et al.Early ontogeny of chromatophores and body color changes of Acanthopagrus schlegelii[J].Progress in Fishery Sciences,2012,33(5):1-7.
[23]YOUKO I.The erythrophore in the larval and adult dorsal skin of the brown frog,Rana ornatiuentris:its differentiation,migration,and pigmentary organelle formation[J].Pigment Cell Research,1998,11:345-354.
[24]HULTMAN KA,BLLDI E H,TEASLEY D C,et al.Defects in ErbB-Dependent establishment of adult melanocyte stem cells reveal independent origins for embryonic and regeneration melanocytes[J].Plos Genetics,2009,5(7):e1000544.