引种杭白菊有效成分含量的分析比较
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摘要
以杭白菊中总糖、黄酮类物质、绿原酸、木犀草苷4种有效成分含量为指标,比较江苏引种杭白菊与原产地浙江桐乡杭白菊的品质差异。采用苯酚硫酸法测定总糖含量,以紫外可见分光光度法测定黄酮类物质的含量,并通过高效液相色谱法测定绿原酸和木犀草苷的含量。结果表明,桐乡杭白菊引种江苏后,4种有效成分的含量均有所增高;与江苏本地杭白菊相比,除总糖含量外,黄酮类物质、绿原酸、木犀草苷的含量较高。由此可知,杭白菊引种江苏有益于杭白菊品质的提高。
Taking the total sugar,flavonoid,chlorogenic acid,luteolin-7-O-glucoside in Chrysanthemum morifolium Ramat as index to compare the quality of species from original habitats Zhejiang Tongxiang. Total sugar content in Chrysanthemum morifolium Ramat is measured by phehol-vitril. The content of flavonoids is analyzed by UV-Vis spectrophotometry. Chlorogenic acid and luteolin-7-O-glucoside is measured by high performance liquid chromatography(HPLC). The results indicate that the content of four effective components increased after Chrysanthemum morifolium Ramat in Tongxiang introduced to Jiangsu. The content of flavonoids,chlorogenic acid and luteolin-7-O-glucoside are higher in Chrysanthemum morifolium Ramat introduced Jiangsu than Jiangsu native,but the content of total sugar is lower. Therefore,The introduction of Chrysanthemum morifolium Ramat is beneficial to the quality improvement.
Taking the total sugar,flavonoid,chlorogenic acid,luteolin-7-O-glucoside in Chrysanthemum morifolium Ramat as index to compare the quality of species from original habitats Zhejiang Tongxiang. Total sugar content in Chrysanthemum morifolium Ramat is measured by phehol-vitril. The content of flavonoids is analyzed by UV-Vis spectrophotometry. Chlorogenic acid and luteolin-7-O-glucoside is measured by high performance liquid chromatography(HPLC). The results indicate that the content of four effective components increased after Chrysanthemum morifolium Ramat in Tongxiang introduced to Jiangsu. The content of flavonoids,chlorogenic acid and luteolin-7-O-glucoside are higher in Chrysanthemum morifolium Ramat introduced Jiangsu than Jiangsu native,but the content of total sugar is lower. Therefore,The introduction of Chrysanthemum morifolium Ramat is beneficial to the quality improvement.
引文
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[13]付炳华,李荣琼,魏世杰,等.杭白菊在昆明的引种栽培表现[J].中国园艺文摘,2013(4):25-27.
[14]张红瑞,李贺敏,张燕.河南引种杭白菊主要有效成分的比较[J].信阳农林学院学报,2016,26(3):89-98.
[15]胡碧波,吴祖帅,蔡君,等.杭白菊中主要黄酮苷的测定及影响因素考察[J].中国中药杂志,2006,31(21):1 772-1 775.
[16]顾瑶华,秦民坚.不同药用菊花品种绿原酸含量的RPHPLC分析[J].植物资源与环境学报,2005,14(2):56-57.
[2]康文艺,黄嬛,廉婷婷,等.杭白菊保肝作用研究[J].天然产物研究与开发,2012,24(11):1 634-1 636.
[3]Wang Tao,Guo Qiaosheng,Mao Pengfei.Flavonoid accumulation during florescence in three Chrysanthemum morifolium Ramat cv.“Hangju”genotypes[J].Biochemical Systematics and Ecology,2014(5):79-83.
[4]周丽,张维,王斌.杭白菊的有效成分及深加工研究进展[J].食品工业,2014,35(6):199-202.
[5]Liang F J,Hu Changfeng,H E Zhengchun,et al.An arabinogalactan from flowers of Chrysanthemum morifolium:structural and bioactivity studies[J].Carbohydrate Research,2014(8):37-41.
[6]Yang Jinfeng,Kim J S,Jeong H J,et al.Determination of antioxidant andα-glucosidase inhibitory activities and luteolin contents of Chrysanthemum morifolium Ramat extracts[J].African Journal of Biotechnology,2011(10):19 197-19 202.
[7]林佑.菊花活性物质及其作用机制研究[J].植物学研究,2012(1):47-53.
[8]Nugroho A,Lim S C,Choi J,et al.Identification and quantification of the sedative and anticonvulsant flavone glycoside from Chrysanthemum boreale[J].Arch.Pharm.Res,2013(4):51-60.
[9]彭钰芳,邹文韬,许传莲.杭白菊中黄酮类化合物及其抑制人源肿瘤细胞增殖活性的研究[J].中国药学杂志,2010,45(19):1 454-1 459.
[10]Lii C K,Lei Y P,Yao H T,et al.Chrysanthemum morifolium Ramat.reduces the oxidized LDL-induced expression of intercellular adhesion molecule-1 and E-selectin in human umbilical vein endothelial cells[J].Journal of Ethnopharmacology,2010(5):213-220.
[11]Feng R,Lu Y J,Qian Y,et al.Inhibition of activator protein-1,NF-B,and MAPKs and induction of phase 2detoxifying enzyme activity by chlorogenic acid*[J].The Journal of Biological Chemistry,2005(30):27 888-27 895.
[12]夏胜平,陈振钦.4个桐乡杭白菊品种的中性表现[J].浙江农业科学,2014,14(3):504-508.
[13]付炳华,李荣琼,魏世杰,等.杭白菊在昆明的引种栽培表现[J].中国园艺文摘,2013(4):25-27.
[14]张红瑞,李贺敏,张燕.河南引种杭白菊主要有效成分的比较[J].信阳农林学院学报,2016,26(3):89-98.
[15]胡碧波,吴祖帅,蔡君,等.杭白菊中主要黄酮苷的测定及影响因素考察[J].中国中药杂志,2006,31(21):1 772-1 775.
[16]顾瑶华,秦民坚.不同药用菊花品种绿原酸含量的RPHPLC分析[J].植物资源与环境学报,2005,14(2):56-57.