雷公藤甲素对肿瘤坏死因子-α介导的HCM心肌细胞生长损伤保护作用研究
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摘要
目的探讨雷公藤甲素对肿瘤坏死因子-α(TNF-α)介导的HCM心肌细胞生长损伤的保护作用。方法采用四唑盐(MTT)比色法检测TNF-α抑制HCM细胞生长的半数抑制浓度(IC50)及雷公藤甲素干预对HCM细胞生长抑制率的影响;采用流式细胞术检测TNF-α联合雷公藤甲素对HCM细胞凋亡率的影响;使用相应试剂盒检测细胞上清液中肌酸激酶(CK)、乳酸脱氢酶(LDH)水平。结果 TNF-α对HCM细胞具有明显的生长抑制和凋亡诱导作用,且血清CK和LDH表达明显升高;低质量浓度雷公藤甲素干预后,TNF-α对HCM细胞的生长抑制和诱导凋亡作用均减弱(P <0. 01),血清CK和LDH表达呈下降趋势。结论低质量浓度雷公藤甲素可减弱TNF-α对HCM细胞的生长抑制和凋亡诱导作用。
Objective To investigate the protective effect of triptolide on TNF-α-mediated damage in the growth of HCM cardiomyocytes.Methods MTT assay was used to detect the IC50 concentration of TNF-α inhibiting the growth of HCM cells, and the effect oftriptolide on inhibition rate of HCM cell growth,the flow cytometry was used to detect the effect of TNF-α combined with triptolide onthe apoptotic rate of HCM cells,and the corresponding kits were used to detect the levels of creatine kinase( CK) and lactate dehydro-genase( LDH) in cell supernatants. Results TNF-α had significant inhibition effect on the growth of HCM cells and significant induc-tion effect on the apoptosis of HCM cells,and the expression of serum CK and LDH was significantly increased. After intervention withlow concentrations of triptolide, the effect of TNF-α on the growth inhibition and apoptosis induction of HCM cells were bothweakened( P < 0. 01),the expression of serum CK and LDH showed a decreasing trend. Conclusion Low concentrations of triptolidecan attenuate the effect of TNF-α on inhibition of growth and apoptosis induction in HCM cells.
Objective To investigate the protective effect of triptolide on TNF-α-mediated damage in the growth of HCM cardiomyocytes.Methods MTT assay was used to detect the IC50 concentration of TNF-α inhibiting the growth of HCM cells, and the effect oftriptolide on inhibition rate of HCM cell growth,the flow cytometry was used to detect the effect of TNF-α combined with triptolide onthe apoptotic rate of HCM cells,and the corresponding kits were used to detect the levels of creatine kinase( CK) and lactate dehydro-genase( LDH) in cell supernatants. Results TNF-α had significant inhibition effect on the growth of HCM cells and significant induc-tion effect on the apoptosis of HCM cells,and the expression of serum CK and LDH was significantly increased. After intervention withlow concentrations of triptolide, the effect of TNF-α on the growth inhibition and apoptosis induction of HCM cells were bothweakened( P < 0. 01),the expression of serum CK and LDH showed a decreasing trend. Conclusion Low concentrations of triptolidecan attenuate the effect of TNF-α on inhibition of growth and apoptosis induction in HCM cells.
引文
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[14]王贝,江振洲,张陆勇.雷公藤甲素毒性及减毒的研究进展[J].药物评价研究,2012,35(3):211-215.
[2] TIAN M,YUAN YC,LI JY,et al. Tumor necrosis factor-αand itsrole as a mediator in myocardial infarction:a brief review[J].Chronic Diseases and Translational Medicine,2015(1):18-26.
[3]王伟,王坤.雷公藤甲素通过ERK通路诱导肺癌A549细胞自噬[J].中国病理生理杂志,2016,32(9):1551-1555.
[4]崔进,陈晓,苏佳灿.雷公藤甲素药理作用研究新进展[J].中国中药杂志,2017,42(14):2655-2658.
[5]韩琳,王洪新,鲁美丽.黄芪多糖通过抑制NF-κB和JNK信号通路减轻LPS诱导的小鼠心肌细胞凋亡[J].中国药理学通报,2018,34(2):243-249.
[6] XU H,YAO Y,SU Z,et al. Endogenous HMGB1 contributes toischemia-reperfusion-induced myocardial apoptosis by potenti-ating the effect of TNF-α/JNK[J]. Am J Physiol Heart CircPhysiol,2011,300(3):H913.
[7]冯惠平,冯惠清,尹博英,等.醛固酮对鼠心肌成纤维细胞诱导型一氧化氮合酶/一氧化氮的调控[J].中华高血压杂志,2010,18(8):769-773.
[8]王玲,王瑞英,张彦莉,等.慢性心力衰竭患者CA125和TNF-α水平的变化及临床意义[J].中国心血管病研究,2014,12(8):724-727.
[9]吕勇刚,徐昕,军,等. TNF-α通过调节神经酰胺水平调控人乳腺癌细胞的增殖[J].细胞与分子免疫学杂志,2017,33(10):1303-1309.
[10]陈将华,郑维威,姜旭东,等.雷公藤甲素通过抑制逆转录病毒HERV-K Np9基因转录诱导人急性T淋巴细胞白血病Jurkat细胞凋亡[J].南方医科大学学报,2015,35(5):702-706.
[11]林君容,林兵,宋洪涛.雷公藤甲素与雷公藤红素的体内药动学研究进展[J].中草药,2016,47(3):528-532.
[12]张海港,童洋飞.雷公藤甲素对心肌细胞CDKN1a表达及心肌肥大的影响[J].中国药理学通报,2015,31(Suppl):195.
[13]童洋飞,郭奉洁,潘夕春,等.雷公藤甲素对大鼠心肌H9c2细胞肥大的抑制作用[J].中国药房,2015,26(7):878-881.
[14]王贝,江振洲,张陆勇.雷公藤甲素毒性及减毒的研究进展[J].药物评价研究,2012,35(3):211-215.