蠲哮汤对哮喘大鼠CysLTs和IL-4、IFN-γ的影响及相关性研究
|
摘要
目的:探讨蠲哮汤对哮喘大鼠半胱氨酸白三烯(CysLTs)和白细胞介素4(IL-4)、干扰素γ(IFN-γ)的影响及相关性。方法:将189只SPF级雌性SD大鼠随机分为正常组(A组),模型组(B组),孟鲁司特钠组(C组),地塞米松组(D组),蠲哮汤低、中、高剂量组(E、F、G组),卵蛋白氢氧化铝溶液致敏,并长期雾化吸入卵蛋白激发哮喘,A组予0.9%氯化钠溶液雾化代替,分别持续1、2、4周,各周结束时,处死每组大鼠9只,检测肺泡灌洗液(BALF)中CysLTs及IFN-γ、IL-4含量,并作相关性分析。结果:CysLTs:与A组同期比较,第1、4周各药物干预组降低,第2周各组升高(P﹤0.05);与B组同期比较,A组、蠲哮汤各剂量组均显著降低(P﹤0.05),各浓度组组间比较无显著性差异;D组低于其他药物干预组(P﹤0.05)。IL-4:与A组同期比较,第1、4周各药物干预组降低,第2周各组升高(P﹤0.05);与B组同期比较,A组、G组、D组均显著降低(P﹤0.05),各浓度组组间比较无显著性差异。CysLTs与IL-4呈正相关性、与IFN-γ呈负相关性。结论:IL-4、IFN-γ与CysLT通路相关,蠲哮汤可能是通过影响CysLTs干预哮喘炎症白三烯通道及通过影响IFN-γ、IL-4干预Th1/Th2失衡而达到治疗哮喘的目的。
Objective: To investigate the effects and correlation of Juanxiao Decoction on CysLTs and IL-4, IFN-γ in asthmatic rats. Methods: A total of 189 SPF female SD rats were randomly divided into normal group(A group), model group(group B), montelukast group(group C), dexamethasone group(D group), low dose of Juanxiao Decoction group(group E), medium dose of Juanziao Decoction(F group) and high dose of Juanziao Decoction(G group), asthma model was established by ovalbumin sensitized aluminum hydroxide solution, and long-term inhalation of ovalbumin, Group A received 0.9% sodium chloride solution atomization respectively for 1, 2 and 4 weeks, at the end of each week, 9 rats in each group were sacrificed and the broncho alveolar lavage fluid(BALF) CysLTs and IFN-gamma, IL-4 content were detected as well as correlation analysis. Results: CysLTs: Compared with group A at the same time, the drug intervention group decreased at the 1 st and 4 th week, and increased at the 2 nd week(P<0.05). Compared with group B at the same time, group A and JuanXiao Tablets dosage groups were significantly lower(P<0.05), there was no significant difference between the concentration groups; group D was lower than other drug intervention groups(P<0.05). IL-4: Compared with group A at the same time, the levels of each drug intervention group decreased at the 1 st and 4 th week, and increased at the 2 nd week(P<0.05). Compared with group B at the same time, the levels of group A, group G and group D decreased significantly(P<0.05), and there was no significant difference among the groups with different concentrations. CysLTs was positively correlated with IL-4 and negatively correlated with IFN-γ. Conclusion: IL-4, IFN-γ and CysLTs channel may be interrelated, Juanxiao Decoction may through the intervention of CysLTs channel and leukotriene inflammation of asthma through the influence of IFN-γ, IL-4 and Th1/Th2 imbalance to treat asthma.
Objective: To investigate the effects and correlation of Juanxiao Decoction on CysLTs and IL-4, IFN-γ in asthmatic rats. Methods: A total of 189 SPF female SD rats were randomly divided into normal group(A group), model group(group B), montelukast group(group C), dexamethasone group(D group), low dose of Juanxiao Decoction group(group E), medium dose of Juanziao Decoction(F group) and high dose of Juanziao Decoction(G group), asthma model was established by ovalbumin sensitized aluminum hydroxide solution, and long-term inhalation of ovalbumin, Group A received 0.9% sodium chloride solution atomization respectively for 1, 2 and 4 weeks, at the end of each week, 9 rats in each group were sacrificed and the broncho alveolar lavage fluid(BALF) CysLTs and IFN-gamma, IL-4 content were detected as well as correlation analysis. Results: CysLTs: Compared with group A at the same time, the drug intervention group decreased at the 1 st and 4 th week, and increased at the 2 nd week(P<0.05). Compared with group B at the same time, group A and JuanXiao Tablets dosage groups were significantly lower(P<0.05), there was no significant difference between the concentration groups; group D was lower than other drug intervention groups(P<0.05). IL-4: Compared with group A at the same time, the levels of each drug intervention group decreased at the 1 st and 4 th week, and increased at the 2 nd week(P<0.05). Compared with group B at the same time, the levels of group A, group G and group D decreased significantly(P<0.05), and there was no significant difference among the groups with different concentrations. CysLTs was positively correlated with IL-4 and negatively correlated with IFN-γ. Conclusion: IL-4, IFN-γ and CysLTs channel may be interrelated, Juanxiao Decoction may through the intervention of CysLTs channel and leukotriene inflammation of asthma through the influence of IFN-γ, IL-4 and Th1/Th2 imbalance to treat asthma.
引文
[1]喻强强,薛汉荣,赵英杰,等.哮喘气道重塑与“痰瘀伏肺”相关性研究.中华中医药杂志,2014,29(7):910-913
[2]洪广祥,张燕萍,黄敬耀,等.蠲哮片治疗哮喘的临床及实验研究.中国中西医结合杂志,1999,19(2):12-15
[3]喻强强,薛汉荣,余建玮,等.疏利气机、涤痰行瘀方(蠲哮汤)对哮喘炎症白三烯通道干预作用初探.中医药通报,2016,15(3):44-46
[4]王文建,杨莉,王西华,等.川芎嗪对支气管哮喘模型气道重塑的影响与机制.中华结核和呼吸杂志,2004,27(12):833-836
[5]王丽新,吴银根.实验性哮喘大鼠气道重塑的形态学变化.中西医结合学报,2003,1(1):62-65
[6]Kotani H,Kishi R,Mouri A,et al.Influence of leukotriene pathway polymorphisms on clinical responses to montelukast in Japanese patients with asthma.J Clin Pharm Ther,2012,37(1):112-116
[7]Haneda Y,Hasegawa S,Hirano R,et al.Leukotriene D4 enhance tumor necrosis factor-alpha inducedvas cular endothelial growth factor production in human monocytes/macrophages.Cytokine,2011,55(1):24-28
[8]Bosse Y,Thompson C,McMathon S,et al.Leukotriene D4induced,epithelial cell-derived tronsforming growth factor betal in human bronchial smooth muscle cell proliferation.Clin Exp Allergy,2008,38(1):113-121
[9]Gibbons F K,Israel E,Deykin A,et al.The combined effects of zafirl ukast,prednisone,andinhaledbudesonide on IL-13and IFN-gamma secretion.J Clin Immunol,2005,25(5):437-444
[10]陈杰,袁岳沙,李琦,等.IL-27对哮喘患儿IFN-γ及IL-4的调节作用.中国当代儿科杂志,2010,12(7):521-523
[11]Yassine A,Paul E.M,Rebecca H,et al.Interferonγmodulates cysteinyl leukotriene receptor-1 expression and function in human airway myocytes.American Journal of Respiratory And Critical Care Medicine,2001,164(11):2098-2101
[12]Masaru Fujii,Hiroshi Tanaka,Shosaku Abe.Interferon-[gamma]Up-regulates expression of cysteinyl leukotriene type 2receptors on eosinophils in asthmatic patients.Chest,2005,28(5):3148-3156
[13]欧维琳,朱春江,蒋林彬.干扰素-γ对支气管上皮细胞表达白三烯受体半胱氨酰白三烯受体1的调节.实用儿科临床志,2010,25(21):1619-1621
[14]Kotowicz K,Dixon G L,Klein N J,et al.Biological function of CD40on human endothelial cells:Co-stimulation with CD40 ligand and interleukin-4 selectively induces expression of vascular cell adhesion moleculIe-1 and P-selectin resulting in preferential adhesion of lymphocyte.Immunology,2000,100(4):441-448
[15]马晓晖,崔振泽,黄燕,等.婴幼儿早期喘息干预前后血、尿白三烯E4水平的变化及其临床意义.实用儿科临床杂志,2012,27(4):280-282
[2]洪广祥,张燕萍,黄敬耀,等.蠲哮片治疗哮喘的临床及实验研究.中国中西医结合杂志,1999,19(2):12-15
[3]喻强强,薛汉荣,余建玮,等.疏利气机、涤痰行瘀方(蠲哮汤)对哮喘炎症白三烯通道干预作用初探.中医药通报,2016,15(3):44-46
[4]王文建,杨莉,王西华,等.川芎嗪对支气管哮喘模型气道重塑的影响与机制.中华结核和呼吸杂志,2004,27(12):833-836
[5]王丽新,吴银根.实验性哮喘大鼠气道重塑的形态学变化.中西医结合学报,2003,1(1):62-65
[6]Kotani H,Kishi R,Mouri A,et al.Influence of leukotriene pathway polymorphisms on clinical responses to montelukast in Japanese patients with asthma.J Clin Pharm Ther,2012,37(1):112-116
[7]Haneda Y,Hasegawa S,Hirano R,et al.Leukotriene D4 enhance tumor necrosis factor-alpha inducedvas cular endothelial growth factor production in human monocytes/macrophages.Cytokine,2011,55(1):24-28
[8]Bosse Y,Thompson C,McMathon S,et al.Leukotriene D4induced,epithelial cell-derived tronsforming growth factor betal in human bronchial smooth muscle cell proliferation.Clin Exp Allergy,2008,38(1):113-121
[9]Gibbons F K,Israel E,Deykin A,et al.The combined effects of zafirl ukast,prednisone,andinhaledbudesonide on IL-13and IFN-gamma secretion.J Clin Immunol,2005,25(5):437-444
[10]陈杰,袁岳沙,李琦,等.IL-27对哮喘患儿IFN-γ及IL-4的调节作用.中国当代儿科杂志,2010,12(7):521-523
[11]Yassine A,Paul E.M,Rebecca H,et al.Interferonγmodulates cysteinyl leukotriene receptor-1 expression and function in human airway myocytes.American Journal of Respiratory And Critical Care Medicine,2001,164(11):2098-2101
[12]Masaru Fujii,Hiroshi Tanaka,Shosaku Abe.Interferon-[gamma]Up-regulates expression of cysteinyl leukotriene type 2receptors on eosinophils in asthmatic patients.Chest,2005,28(5):3148-3156
[13]欧维琳,朱春江,蒋林彬.干扰素-γ对支气管上皮细胞表达白三烯受体半胱氨酰白三烯受体1的调节.实用儿科临床志,2010,25(21):1619-1621
[14]Kotowicz K,Dixon G L,Klein N J,et al.Biological function of CD40on human endothelial cells:Co-stimulation with CD40 ligand and interleukin-4 selectively induces expression of vascular cell adhesion moleculIe-1 and P-selectin resulting in preferential adhesion of lymphocyte.Immunology,2000,100(4):441-448
[15]马晓晖,崔振泽,黄燕,等.婴幼儿早期喘息干预前后血、尿白三烯E4水平的变化及其临床意义.实用儿科临床杂志,2012,27(4):280-282