摘要
本试验旨在研究柠檬酸对小鼠脂肪细胞甘油三酯(TG)及其代谢产物含量的影响。将小鼠3T3-L1前脂细胞诱导成成熟的脂肪细胞作为研究对象,在诱导培养第14天,加入含0(对照组)、20(试验Ⅰ组)、50(试验Ⅱ组)、200μmol/L的柠檬酸完全培养基(试验Ⅲ组)培养细胞,于0、36、72 h收集细胞检测TG合成和分解代谢中关键限速酶及代谢产物的含量。结果表明:在36和72 h,试验Ⅰ、Ⅱ、Ⅲ组TG含量显著高于对照组(P<0.05);各试验组醛缩酶(FDA)、乙酰辅酶A羧化酶(ACC)和脂肪酸合成酶(FAS)含量均显著或极显著高于对照组(P<0.05或P<0.01);各试验组激素敏感脂肪酶(HSL)、肉碱脂酰转移酶1(CPT1)、游离脂肪酸(FFA)及乙酰辅酶A含量均极显著低于对照组(P<0.01)。结果提示,柠檬酸能够促进小鼠脂肪细胞TG的合成和沉积,能显著或极显著提高TG合成代谢中FDA、ACC、FAS产物的含量,极显著降低TG分解代谢中HSL、CPT1、FFA及乙酰辅酶A产物的含量,20μmol/L为最佳剂量。
The purpose of this study was to investigate the effects of citric acid on contents of triglyceride and its metabolites of mouse adipocytes. Firstly inoculated the mouse 3 T3-L1 cells into mature adipocytes as the test subject,the cells were cultured in complete mediums containing 0( CK group),20( test group Ⅰ),50( test group Ⅱ) and 200 μmol/L citric acid( test group Ⅲ) on the 14 th day,respectively. The cells were collected at 0,36 and 72 h for the detection of key rate-limiting enzyme contents in TG synthesis and catabolism,and TG metabolite contents,respectively. The results showed as follows: at 36 an 72 h,TG content in test groupsⅠ,Ⅱ and Ⅲ was significantly higher than that in CK group( P<0.05). The contents of aldolase( FDA),acetyl-Co A carboxylase( ACC) and fatty acid synthetase( FAS) in test groups were significantly higher than those in CK group( P< 0.05 or P< 0.01). The contents of hormone-sensitive lipase( HSL),carnitine palmityl transferase 1( CPT1),free fatty acids( FFA) and acetyl Co A in test groups were significantly lower than those in control group( P< 0.01). In conclusion,citric acid can promote the synthesis and deposition of TG of mouse adipocytes,significantly increase the contents of FDA,ACC,and FAS in TG synthesis,and significantly reduce the contents of HSL,CPT1,FFA,acetyl Co A in TG disintegration; the optimal dosage of citric acid is 20 μmol/L.
引文
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