响应面法优化微波辅助提取蛤蟆草蛋白工艺研究
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摘要
以蛤蟆草为原料,以蛋白提取量为指标,采用微波辅助提取蛤蟆草中蛋白。分别研究了液料比、微波功率、微波提取时间、乙醇体积分数对蛋白提取量的影响,通过响应面试验优化了提取工艺。结果表明,蛤蟆草蛋白提取的较佳工艺条件是:液料比50︰1 mL/g,微波功率460 W,微波提取时间3 min,乙醇体积分数60%。在该条件下,蛤蟆草蛋白的提取量为1.86 mg/g。
With Bothriospermum chinense as raw material, extraction content of protein as the index, Bothriospermum chinenseprotein was extracted through microwave-assisted method. The effects of liquid-solid ratio, microwave power, microwave extraction time, and ethanol concentration on the extraction of protein were studied. The results showed that the optimum extraction conditions by response surface method were: liquid-solid ratio 50︰1 mL/g, microwave power 460 W, microwave extraction time 3 min, and ethanol concentration 60%. Under these conditions, the extraction of Bothriospermum chinense protein was 1.86 mg/g.
With Bothriospermum chinense as raw material, extraction content of protein as the index, Bothriospermum chinenseprotein was extracted through microwave-assisted method. The effects of liquid-solid ratio, microwave power, microwave extraction time, and ethanol concentration on the extraction of protein were studied. The results showed that the optimum extraction conditions by response surface method were: liquid-solid ratio 50︰1 mL/g, microwave power 460 W, microwave extraction time 3 min, and ethanol concentration 60%. Under these conditions, the extraction of Bothriospermum chinense protein was 1.86 mg/g.
引文
[1]王海桥,王筱婧,王光花,等.蛤蟆草对巨噬细胞炎症因子分泌的影响及免疫调节作用研究[J].免疫学杂志, 2017,33(8):668-677.
[2]余磊,于敬海,彭湘君,等.蛤蟆草化学成分的研究[J].哈尔滨医科大学学报, 2006, 40(5):416-419.
[3]刘慧清,王国凯,林彬彬,等.荔枝草全草乙醇提取物的化学成分分析[J].植物资源与环境学报, 2013, 22(2):111-113.
[4]隋秀竹,李欣.蛤蟆草治疗肺癌的疗效观察[J].中国民间疗法, 2002, 10(9):40-41.
[5]杨梅.蛤蟆草液治宫颈糜烂[J].中国民间疗法, 2008(11):61.
[6]宋云红,李秀芹,刘新霞.蛤蟆草治疗痔疮50例[J].中国民间疗法, 2002(7):44-45.
[7]马瑜红,李玲,欧阳静萍,等.荔枝草抗炎作用的实验研究[J].时珍国医国药, 2008, 19(9):2209-2210.
[8]余梦妮,包婉君,谌馥佳,等.菊芋叶蛋白提取工艺研究及氨基酸分析[J].草业科学, 2015, 32(1):125-131.
[9]朱天明,陈泠伶,杨潇,等.纤维素酶辅助提取桑叶中叶蛋白的工艺[J].西华大学学报(自然科学版), 2016, 35(2):77-81.
[10]王震,乔天磊,霍乃蕊,等.植物叶蛋白提取及应用前景[J].山西农业科学, 2015, 43(12):1727-1731.
[11]施曼,高岩,易能,等.植物叶蛋白提取及脱色研究进展[J].食品工业科技, 2017, 38(21):342-351.
[12]谢正军.苜蓿叶蛋白和酶法制备抗氧化肽的研究[D].无锡:江南大学, 2009.
[13]许英一,江成英,吴红艳.超声波辅助碱法提取紫花苜蓿叶蛋白的工艺研究[J].食品工业, 2013, 34(8):14-16.
[14]刘晓娟.苜蓿总黄酮与叶蛋白联合提取方法探索[D].长沙:湖南农业大学, 2016.
[15]王震,乔天磊,霍乃蕊,等.植物叶蛋白提取方法及研究进展[J].山西农业科学, 2016, 44(1):126-130.
[16] BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry,1976, 72(1/2):248-254.
[17]黄秋伟,彭欣怡,黄惠芳.辣木叶蛋白超声辅助盐提取工艺初步研究[J].农业研究与应用, 2016(3):48-51.
[2]余磊,于敬海,彭湘君,等.蛤蟆草化学成分的研究[J].哈尔滨医科大学学报, 2006, 40(5):416-419.
[3]刘慧清,王国凯,林彬彬,等.荔枝草全草乙醇提取物的化学成分分析[J].植物资源与环境学报, 2013, 22(2):111-113.
[4]隋秀竹,李欣.蛤蟆草治疗肺癌的疗效观察[J].中国民间疗法, 2002, 10(9):40-41.
[5]杨梅.蛤蟆草液治宫颈糜烂[J].中国民间疗法, 2008(11):61.
[6]宋云红,李秀芹,刘新霞.蛤蟆草治疗痔疮50例[J].中国民间疗法, 2002(7):44-45.
[7]马瑜红,李玲,欧阳静萍,等.荔枝草抗炎作用的实验研究[J].时珍国医国药, 2008, 19(9):2209-2210.
[8]余梦妮,包婉君,谌馥佳,等.菊芋叶蛋白提取工艺研究及氨基酸分析[J].草业科学, 2015, 32(1):125-131.
[9]朱天明,陈泠伶,杨潇,等.纤维素酶辅助提取桑叶中叶蛋白的工艺[J].西华大学学报(自然科学版), 2016, 35(2):77-81.
[10]王震,乔天磊,霍乃蕊,等.植物叶蛋白提取及应用前景[J].山西农业科学, 2015, 43(12):1727-1731.
[11]施曼,高岩,易能,等.植物叶蛋白提取及脱色研究进展[J].食品工业科技, 2017, 38(21):342-351.
[12]谢正军.苜蓿叶蛋白和酶法制备抗氧化肽的研究[D].无锡:江南大学, 2009.
[13]许英一,江成英,吴红艳.超声波辅助碱法提取紫花苜蓿叶蛋白的工艺研究[J].食品工业, 2013, 34(8):14-16.
[14]刘晓娟.苜蓿总黄酮与叶蛋白联合提取方法探索[D].长沙:湖南农业大学, 2016.
[15]王震,乔天磊,霍乃蕊,等.植物叶蛋白提取方法及研究进展[J].山西农业科学, 2016, 44(1):126-130.
[16] BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry,1976, 72(1/2):248-254.
[17]黄秋伟,彭欣怡,黄惠芳.辣木叶蛋白超声辅助盐提取工艺初步研究[J].农业研究与应用, 2016(3):48-51.