植物多糖对断奶仔猪小肠黏膜形态及肠黏膜屏障功能的影响
|
摘要
旨在探讨断奶仔猪饲粮中单独或联合添加黄芪多糖、牛膝多糖及白术多糖对其小肠黏膜形态及肠黏膜屏障功能的影响。选取256头35日龄的杜长大三元杂交断奶仔猪,随机分为8个组,每组4个重复,每个重复8头猪,采用三因素两水平(2×2×2)试验设计,饲粮中黄芪多糖、白术多糖和牛膝多糖的添加水平均为0,800mg/kg,试验共进行28d。结果显示:植物多糖能显著提高断奶仔猪十二指肠、空肠和回肠的黏膜绒毛高度,提高绒腺比(P<0.05),3种多糖联用效果最适。与对照组相比,多糖组断奶仔猪回肠V/C比值显著提高(P<0.05),各单一多糖组之间差异不显著(P>0.05),3种多糖联合添加与两两添加及单一添加相比显著提高了V/C比值(P<0.05)。多糖组断奶仔猪血清中二胺氧化酶(DAO)的活性、血清中内毒素(ET)含量及D-乳糖的含量均显著降低(P<0.05),3种多糖联用效果最适。多糖能显著提高断奶仔猪肠道黏膜Occludin、Claudin和ZO-1的基因表达量(P<0.05)。单一添加黄芪多糖、白术多糖及牛膝多糖能显著提高断奶仔猪肠道钠-葡萄糖共转运载体1(SGLT1)的mRNA相对表达量(P<0.05),对葡萄糖转运载体2(GLUT2)mRNA相对表达量有提高的趋势,但差异不显著(P>0.05)。多糖两两联用及3种多糖联合添加能显著提高断奶仔猪肠道SGLT1和GLUT2mRNA相对表达量(P<0.05)。结果表明,在断奶仔猪饲粮中添加植物多糖可显著改善断奶仔猪肠道黏膜形态结构和肠道的通透性,其机制可能与植物多糖改善断奶仔猪肠黏膜屏障功能作用有关。
This experiment was conducted to investigate the effects of Astragalus polysaccharides(APS),atractylodes macrocephala polysaccharides(AMP)and achyranthes bidentata polysaccharides(ABP)on intestinal mucosal morphology and barrier in weaned piglets.A total of two hundred and fifty six 35-day-old Duroc×Landrace×Yorkshire(DLY)crossbred piglets were randomly divided into 8treatments and each treatment had 4replicates with 8piglets per replicate.A 2×2×2factorial trial was conducted and the amount of APS,AMP and ABP were added in 0and800mg/kg for 28 days.The results showed as follows:polysaccharides can significantly increase mucosal villus height in duodenum,jejunum and ileum of weaned piglets,increase flock ratio(P<0.05),and combined three polysaccharides significantly increased weaning piglet V/C ratio of ileum(P<0.05).There was no significant difference in the V/C ratio among the polysaccharides in each single addition and was significantly higher than that of contrl.The single addition of APS,AMP and ABP significantly increased the relative expression of mRNA(P<0.05)in the intestinal SGLT1 of weaned piglets,and the relative expression of GLUT2 mRNA increased,but the difference was not significant(P>0.05).Any two or three polysaccharides combined could significantly increase the relative expression of SGLT1 and GLUT2mRNA in weaned piglets(P<0.05).The experiment showed that the addition of botanlic polysaccharide in weaned piglets can significantly improve the intestinal mucosal morphology and intestinal permeability of weaned piglets and reduce the damage of weaned stress to intestines in piglets,its may be related to the function of plant polysaccharide to enhance the intestinal mucosal barrier function of weanling piglets.
This experiment was conducted to investigate the effects of Astragalus polysaccharides(APS),atractylodes macrocephala polysaccharides(AMP)and achyranthes bidentata polysaccharides(ABP)on intestinal mucosal morphology and barrier in weaned piglets.A total of two hundred and fifty six 35-day-old Duroc×Landrace×Yorkshire(DLY)crossbred piglets were randomly divided into 8treatments and each treatment had 4replicates with 8piglets per replicate.A 2×2×2factorial trial was conducted and the amount of APS,AMP and ABP were added in 0and800mg/kg for 28 days.The results showed as follows:polysaccharides can significantly increase mucosal villus height in duodenum,jejunum and ileum of weaned piglets,increase flock ratio(P<0.05),and combined three polysaccharides significantly increased weaning piglet V/C ratio of ileum(P<0.05).There was no significant difference in the V/C ratio among the polysaccharides in each single addition and was significantly higher than that of contrl.The single addition of APS,AMP and ABP significantly increased the relative expression of mRNA(P<0.05)in the intestinal SGLT1 of weaned piglets,and the relative expression of GLUT2 mRNA increased,but the difference was not significant(P>0.05).Any two or three polysaccharides combined could significantly increase the relative expression of SGLT1 and GLUT2mRNA in weaned piglets(P<0.05).The experiment showed that the addition of botanlic polysaccharide in weaned piglets can significantly improve the intestinal mucosal morphology and intestinal permeability of weaned piglets and reduce the damage of weaned stress to intestines in piglets,its may be related to the function of plant polysaccharide to enhance the intestinal mucosal barrier function of weanling piglets.
引文
[1]PEDRO L G C,FRANZ D G,MAICON S,et al.Effects of dietary supplementation of red pepper(Schinus terebinthifolius Raddi)essential oil on performance,small intestinal morphology and microbial counts of weanling pigs[J].J Sci Food Agric,2018,98(2):541-548.
[2]MAO X F,PIAO X S,LAI C H,et al.Effects of glucan obtained from the Chinese herb Astragalus Membranaceus and lipopolysaccharide challenge on performance,immunological,adrenal and somatotropic responses of weaned pigs[J].J Anim Sci,2005,83(12):2775-2782.
[3]BOUDRY G,PERON V,HLI L.Weaning induces both transient and long-lasting modifications of absorptive,secretory,and barrier properties of piglet intestine[J].J Nutr,2004,134(9):2256-2262.
[4]GROSCHWITZ K R,HOGAN S P.Intestinal barrier function:molecular regulation and disease pathogenesis[J].J Allergy Clin Immunol,2009,124(1):3-20.
[5]WIJTTEN P J,VANDER M J,VERSTEGEN M W.Intestinal barrier function and absorption in pigs after weaning:a review[J].Br J Nutr,2011,105(7):967-981.
[6]MCLAMB B L,GIBSON A J,OVERMAN E L.Early weaning stress in pigs impairs innate mucosal immune responses to enterotoxigenic E.coli challenge and exacerbates intestinal injury and clinical disease[J].PLoS One,2013,8(4):e59838.
[7]COSTA L S,FIDELIS G P,CORDEIRO S L,et al.Biological activities of sulfated polysaccharides from tropical seaweeds[J].Biomedicine Pharmacotherapy,2010,64(1):21-28.
[8]YIN H,WANG Y,WANG Y,et al.Purification,characterization and immuno-modulating properties of polysaccharides isolated from flammulina velutipes mycelium[J].Am J Chin Med,2010,38(1):191-204.
[9]伍婷婷,李茹柳,曾丹,等.白术多糖调控钙离子以促进细胞迁移及E-钙黏蛋白表达的研究[J].中药新药与临床药理,2017,28(2):145-150.
[10] CHEN Q H,LIU Z Y,HE J H,et al.Achyranthes bidentata polysaccharide enhances immune response in weaned piglets[J].Immunopharmacol Immunotoxicol,2009,31(2):253-260.
[11] XU Z R,HU C H,XIA M S,et al.Effects of dietary fructooligosaccharide on digestive enzyme activities,intestinal microflora and morphology of male broilers[J].Poult Sci,2003,82(6):1030-1036.
[12] RAJPUT N,MUHAMMAD N,YAN R.Effect of dietary supplementation of curcumin on growth performance,intestinal morphology and nutrients utilization of broiler chicks[J].J Poult Sci,2013,50(1):44-52.
[13]陶浩.黄芪多糖对肉仔鸡肠道发育及抗氧化能力的影响[D].吉林长春:吉林农业大学,2012.
[14] LALLES J P,BOUDRY G,FAVIER C.Gut function and dysfunction in young pigs:physiology[J].Physiology,2004,53(4):301-316.
[15]赵燕飞,汪以真.白术、微米白术和白术多糖对断奶仔猪生长性能和肠道形态及微生态区系的影响[J].中国畜牧杂志,2015,51(1):65-69.
[16] HU C,WEN P,LIU J.Effects of polysaccharides from rhizoma atractylodis macrocephalae and from radix astragali on polyamine-mediated K+channels in the IEC-6migration[J].Cell Biol Inrernational,2010,34(8):S2.
[17]陈清华,贺建华,刘祝英.牛膝多糖对仔猪肠道微生物及小肠黏膜形态的影响[J].湖南农业大学学报(自然科学版),2007,33(6):556-559.
[18] ZHAO Y,QIN G,SUN Z,et al.Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets[J].Int J Mol Sci,2011,12(12):8502-8512.
[19] SMITH F,CLARK J E,OVERMAN B L,et al.Early weaning stress impairs development of mucosal barrier function in the porcine intestine[J].Am J Physiol Gastrointestinal Liver Physiol,2010,298(3):352-363.
[20]尚庆辉.苜蓿多糖对仔猪生产性能的影响及其机理的研究[D].山东泰安:山东农业大学,2016.
[21] HAN J,XU Y,YANG D,et al.Effect of Polysaccharides from acanthopanax senticosus on intestinal mucosal barrier of Escherichia coli lipopolysaccharide challenged mice[J].Asian-Australasian J Anim Sci,2016,29(1):134-141.
[22] REN W,YIN J,WU M,et al.Serum amino acids profile and the beneficial effects of L-arginine or L-glutamine supplementation in dextran sulfate sodium colitis[J].PLoS One,2014,9(2):e88335.
[23] HU C H,XIAO K,LUAN Z S,et al.Early weaning increases intestinal permeability,alters expression of cytokine and tight junction proteins,and activates mitogen-activated protein kinases in pigs[J].J Anim Sci,2013,91(3):1094-1101.
[24] WALKER W A.Development of the intestinal mucosal barrier[J].J Pediatric Gastroenterol Nutr,2002,34(1):33-39.
[25] LI X T,ZHANG Y K,KUANG H X,et al.Mitochondrial protection and anti-aging activity of Astragalus polysaccharides and their potential mechanism[J].Int J Mol Sci,2012,13(12):1747-1761.
[26] SHEN L,WEBER C R,RALEIGH D R,et al.Tight junction pore and leak pathways:a dynamic duo[J].Annual Rev Physiol,2011,73(1):283-309.
[27] HAN Q,WU Z,HUANG B,et al.Extraction,antioxidant and antibacterial activities of broussonetia papyrifera fruits polysaccharides[J].Int J Biol Macromolecules,2016,92(11):116-124.
[28]江昌盛,周彤,张倩,等.香菇多糖对大肠杆菌攻毒大鼠空肠形态结构、上皮细胞数量和紧密连接蛋白表达的影响[J].动物营养学报,2017,29(10):3592-3597.
[29]孙豪.玉屏风多糖合生元对獭兔免疫功能和肠道菌群结构的影响[D].四川雅安:四川农业大学,2016.
[30] WANG X F,LI Y L,YANG X J,et al.Astragalus polysaccharide reduces inflammatory response by decreasing permeability of LPS-infected Caco2cells[J].Int J Biological Macromolecules,2013,61(10):347-352.
[31]雷新宇.种公鸡日粮添加黄芪多糖调控种公鸡和F1代肉仔鸡肠道功能的研究[D].陕西杨凌:西北农林科技大学,2016.
[32] ABBASI N N,PURSLOW P P,TOSH S M,et al.Oatβ-glucan depresses SGLT1-and GLUT2-mediated glucose transport in intestinal epithelial cells(IEC-6)[J].Nutrition Res,2016,36(6):541-552.
[33] YASUTAKE H,GODA T,TAKASE S.Dietary regulation of sucraseisomaltase gene expression in rat jejunum[J].Biochim Biophys Acta,1995,1243(2):270-276.
[34]吴云鹏.植物乳杆菌调节猪肠上皮细胞屏障功能和转运载体的研究[D].广东广州:华南农业大学,2016.
[35] YANG C,ALBIN D M,WANG Z,et al.Apical Na+-D-glucose cotransporter 1(SGLT1)activity and protein abundance are expressed along the jejunal cryptvillus axis in the neonatal pig[J].Am J Physiol Gastrointest Liver Physiol,2011,300(1):60-70.
[36] KELLETT G L,BROTLAROCHE E,MACE O J,et al.Sugar absorption in the intestine:the role of GLUT2[J].Annu Rev Nutr,2008,33(28):35-54.
[37] SCHEEPERS A,JOOST H G,SCHURMANN A.The glucose transporter families SGLT and GLUT:molecular basis of normal and aberrant function[J].J Parenteral Enteral Nutr,2004,28(5):364-371.
[38]刘磊.黄芪多糖调控肉鸡免疫应激反应研究[D].陕西杨凌:西北农林科技大学,2015.
[39] GARRIGA C,ROVIRA N,MORETM,et al.Expression of Na+-D-glucose cotransporter in brush border membrane of the chicken intestine[J].Am J Physiol,1999,276(2):627-631.
[40]张敏,孙权,周嘉恒,等.维持机体糖稳态及葡萄糖的感受器:小肠葡萄糖转运体2研究进展[J].中国糖尿病杂志,2016,8(9):571-573.
[41]谢红兵,邹云,刘丽莉,等.植物多糖对断奶仔猪生长性能及肠道内环境的影响[J].动物营养学报,2018,30(7):2662-2671.
[2]MAO X F,PIAO X S,LAI C H,et al.Effects of glucan obtained from the Chinese herb Astragalus Membranaceus and lipopolysaccharide challenge on performance,immunological,adrenal and somatotropic responses of weaned pigs[J].J Anim Sci,2005,83(12):2775-2782.
[3]BOUDRY G,PERON V,HLI L.Weaning induces both transient and long-lasting modifications of absorptive,secretory,and barrier properties of piglet intestine[J].J Nutr,2004,134(9):2256-2262.
[4]GROSCHWITZ K R,HOGAN S P.Intestinal barrier function:molecular regulation and disease pathogenesis[J].J Allergy Clin Immunol,2009,124(1):3-20.
[5]WIJTTEN P J,VANDER M J,VERSTEGEN M W.Intestinal barrier function and absorption in pigs after weaning:a review[J].Br J Nutr,2011,105(7):967-981.
[6]MCLAMB B L,GIBSON A J,OVERMAN E L.Early weaning stress in pigs impairs innate mucosal immune responses to enterotoxigenic E.coli challenge and exacerbates intestinal injury and clinical disease[J].PLoS One,2013,8(4):e59838.
[7]COSTA L S,FIDELIS G P,CORDEIRO S L,et al.Biological activities of sulfated polysaccharides from tropical seaweeds[J].Biomedicine Pharmacotherapy,2010,64(1):21-28.
[8]YIN H,WANG Y,WANG Y,et al.Purification,characterization and immuno-modulating properties of polysaccharides isolated from flammulina velutipes mycelium[J].Am J Chin Med,2010,38(1):191-204.
[9]伍婷婷,李茹柳,曾丹,等.白术多糖调控钙离子以促进细胞迁移及E-钙黏蛋白表达的研究[J].中药新药与临床药理,2017,28(2):145-150.
[10] CHEN Q H,LIU Z Y,HE J H,et al.Achyranthes bidentata polysaccharide enhances immune response in weaned piglets[J].Immunopharmacol Immunotoxicol,2009,31(2):253-260.
[11] XU Z R,HU C H,XIA M S,et al.Effects of dietary fructooligosaccharide on digestive enzyme activities,intestinal microflora and morphology of male broilers[J].Poult Sci,2003,82(6):1030-1036.
[12] RAJPUT N,MUHAMMAD N,YAN R.Effect of dietary supplementation of curcumin on growth performance,intestinal morphology and nutrients utilization of broiler chicks[J].J Poult Sci,2013,50(1):44-52.
[13]陶浩.黄芪多糖对肉仔鸡肠道发育及抗氧化能力的影响[D].吉林长春:吉林农业大学,2012.
[14] LALLES J P,BOUDRY G,FAVIER C.Gut function and dysfunction in young pigs:physiology[J].Physiology,2004,53(4):301-316.
[15]赵燕飞,汪以真.白术、微米白术和白术多糖对断奶仔猪生长性能和肠道形态及微生态区系的影响[J].中国畜牧杂志,2015,51(1):65-69.
[16] HU C,WEN P,LIU J.Effects of polysaccharides from rhizoma atractylodis macrocephalae and from radix astragali on polyamine-mediated K+channels in the IEC-6migration[J].Cell Biol Inrernational,2010,34(8):S2.
[17]陈清华,贺建华,刘祝英.牛膝多糖对仔猪肠道微生物及小肠黏膜形态的影响[J].湖南农业大学学报(自然科学版),2007,33(6):556-559.
[18] ZHAO Y,QIN G,SUN Z,et al.Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets[J].Int J Mol Sci,2011,12(12):8502-8512.
[19] SMITH F,CLARK J E,OVERMAN B L,et al.Early weaning stress impairs development of mucosal barrier function in the porcine intestine[J].Am J Physiol Gastrointestinal Liver Physiol,2010,298(3):352-363.
[20]尚庆辉.苜蓿多糖对仔猪生产性能的影响及其机理的研究[D].山东泰安:山东农业大学,2016.
[21] HAN J,XU Y,YANG D,et al.Effect of Polysaccharides from acanthopanax senticosus on intestinal mucosal barrier of Escherichia coli lipopolysaccharide challenged mice[J].Asian-Australasian J Anim Sci,2016,29(1):134-141.
[22] REN W,YIN J,WU M,et al.Serum amino acids profile and the beneficial effects of L-arginine or L-glutamine supplementation in dextran sulfate sodium colitis[J].PLoS One,2014,9(2):e88335.
[23] HU C H,XIAO K,LUAN Z S,et al.Early weaning increases intestinal permeability,alters expression of cytokine and tight junction proteins,and activates mitogen-activated protein kinases in pigs[J].J Anim Sci,2013,91(3):1094-1101.
[24] WALKER W A.Development of the intestinal mucosal barrier[J].J Pediatric Gastroenterol Nutr,2002,34(1):33-39.
[25] LI X T,ZHANG Y K,KUANG H X,et al.Mitochondrial protection and anti-aging activity of Astragalus polysaccharides and their potential mechanism[J].Int J Mol Sci,2012,13(12):1747-1761.
[26] SHEN L,WEBER C R,RALEIGH D R,et al.Tight junction pore and leak pathways:a dynamic duo[J].Annual Rev Physiol,2011,73(1):283-309.
[27] HAN Q,WU Z,HUANG B,et al.Extraction,antioxidant and antibacterial activities of broussonetia papyrifera fruits polysaccharides[J].Int J Biol Macromolecules,2016,92(11):116-124.
[28]江昌盛,周彤,张倩,等.香菇多糖对大肠杆菌攻毒大鼠空肠形态结构、上皮细胞数量和紧密连接蛋白表达的影响[J].动物营养学报,2017,29(10):3592-3597.
[29]孙豪.玉屏风多糖合生元对獭兔免疫功能和肠道菌群结构的影响[D].四川雅安:四川农业大学,2016.
[30] WANG X F,LI Y L,YANG X J,et al.Astragalus polysaccharide reduces inflammatory response by decreasing permeability of LPS-infected Caco2cells[J].Int J Biological Macromolecules,2013,61(10):347-352.
[31]雷新宇.种公鸡日粮添加黄芪多糖调控种公鸡和F1代肉仔鸡肠道功能的研究[D].陕西杨凌:西北农林科技大学,2016.
[32] ABBASI N N,PURSLOW P P,TOSH S M,et al.Oatβ-glucan depresses SGLT1-and GLUT2-mediated glucose transport in intestinal epithelial cells(IEC-6)[J].Nutrition Res,2016,36(6):541-552.
[33] YASUTAKE H,GODA T,TAKASE S.Dietary regulation of sucraseisomaltase gene expression in rat jejunum[J].Biochim Biophys Acta,1995,1243(2):270-276.
[34]吴云鹏.植物乳杆菌调节猪肠上皮细胞屏障功能和转运载体的研究[D].广东广州:华南农业大学,2016.
[35] YANG C,ALBIN D M,WANG Z,et al.Apical Na+-D-glucose cotransporter 1(SGLT1)activity and protein abundance are expressed along the jejunal cryptvillus axis in the neonatal pig[J].Am J Physiol Gastrointest Liver Physiol,2011,300(1):60-70.
[36] KELLETT G L,BROTLAROCHE E,MACE O J,et al.Sugar absorption in the intestine:the role of GLUT2[J].Annu Rev Nutr,2008,33(28):35-54.
[37] SCHEEPERS A,JOOST H G,SCHURMANN A.The glucose transporter families SGLT and GLUT:molecular basis of normal and aberrant function[J].J Parenteral Enteral Nutr,2004,28(5):364-371.
[38]刘磊.黄芪多糖调控肉鸡免疫应激反应研究[D].陕西杨凌:西北农林科技大学,2015.
[39] GARRIGA C,ROVIRA N,MORETM,et al.Expression of Na+-D-glucose cotransporter in brush border membrane of the chicken intestine[J].Am J Physiol,1999,276(2):627-631.
[40]张敏,孙权,周嘉恒,等.维持机体糖稳态及葡萄糖的感受器:小肠葡萄糖转运体2研究进展[J].中国糖尿病杂志,2016,8(9):571-573.
[41]谢红兵,邹云,刘丽莉,等.植物多糖对断奶仔猪生长性能及肠道内环境的影响[J].动物营养学报,2018,30(7):2662-2671.