新精神活性物质的三维荧光光谱检测方法研究
|
摘要
新精神活性物质的合成和滥用问题日益严峻。本文提出将三维荧光光谱法用于4种常见新精神活性物质快速检测分析。结果表明,2,5-二甲氧基苯乙胺(2C-H)、3,4-亚甲基二氧甲基卡西酮(MDMC)、亚甲基二氧吡咯戊酮(MDPV)和氯胺酮有不同的荧光光谱特征,荧光强度最大处的荧光峰位置(λex/λem)分别为284/326、282/430、282/430、270/392nm。2C-H荧光分析法灵敏度最高,氯胺酮的灵敏度最低。在尿样内源荧光物质干扰下,利用三维荧光光谱法与平行因子算法相结合提取新精神活性物质的荧光信号,解析出的激发光谱和发射光谱与标准品的实测光谱较为一致,样品添加回收率范围为91.3%～111.2%。
New psychoactive substances(NPS) are increasing to be a grim problem with their synthesis and abuse. Here,three-dimensional fluorescent spectroscopy was proposed for rapid determination of four typical new psychoactive substances(2,5-dimethoxyphenethylamine or 2 C-H, 3,4-methylenedioxy-N-methylcathinone or MDMC, methylenedioxypyrovalerone or MDPV, and ketamine). Results showed that the fluorescent characteristics of the interested substances were different, the peak maximal fluorescence intensity(λex/λem) appeared at 284/326, 282/430, 282/430 and 270/392 nm for 2 C-H, MDMC, MDPV and ketamine, respectively, with 2 C-H being most sensitive yet ketamine lowest sensitivity. Deducted of the interference from the fluorescent substance in urine, the fluorescence signals of the interested NPS were extracted with parallel factor analysis(PARAFAC) into each three-dimensional fluorescence. The PARAFAC-resolved fluorescent spectra of the four typical NPS in urine were consistent with those of the standard reference materials, with the recoveries among the range of 91.3%～111.2%.
New psychoactive substances(NPS) are increasing to be a grim problem with their synthesis and abuse. Here,three-dimensional fluorescent spectroscopy was proposed for rapid determination of four typical new psychoactive substances(2,5-dimethoxyphenethylamine or 2 C-H, 3,4-methylenedioxy-N-methylcathinone or MDMC, methylenedioxypyrovalerone or MDPV, and ketamine). Results showed that the fluorescent characteristics of the interested substances were different, the peak maximal fluorescence intensity(λex/λem) appeared at 284/326, 282/430, 282/430 and 270/392 nm for 2 C-H, MDMC, MDPV and ketamine, respectively, with 2 C-H being most sensitive yet ketamine lowest sensitivity. Deducted of the interference from the fluorescent substance in urine, the fluorescence signals of the interested NPS were extracted with parallel factor analysis(PARAFAC) into each three-dimensional fluorescence. The PARAFAC-resolved fluorescent spectra of the four typical NPS in urine were consistent with those of the standard reference materials, with the recoveries among the range of 91.3%～111.2%.
引文
[1]张黎,张拓.新精神活性物质的滥用危害与防控问题研究--以构建我国禁毒防控体系为视角[J].中国人民公安大学学报(社会科学版),2013,29(4):88-96.
[2]WIERGOWSKI M,ASZYK J,KALISZAN M,et al.Identification of novel psychoactive substances 25B-NBOMe and4-CMC in biological material using HPLC-Q-TOF-MS and their quantification in blood using UPLC-MS/MS in case of severe intoxications[J].Journal of Chromatography B,2017,1041-1042(1):1-10.
[3]GUIRGUIS A,GIROTTO S,BERTI B,et al.Identification of new psychoactive substances(NPS)using handheld Raman spectroscopy employing both 785 and 1064 nm laser sources[J].Forensic Science International,2017,273(4):113-123.
[4]孟鑫,刘培培,乔艳玲,等.尿液中甲卡西酮类新精神活性物质的GC-MS检测法[J].警察技术,2017(3):85-87.
[5]MAZINA J,SPILJOVA A,VAHER M,et al.A rapid capillary electrophoresis method with LED-induced native fluorescence detection for the analysis of cannabinoids in oral fluid[J].Analytical Methods,2015,7(18):7741-7747.
[6]王晓燕,陈仁文.典型食品果汁中百菌清农药残留荧光检测建模研究[J].光谱学与光谱分析,2017,27(3):794-798.
[7]WANG X,LI B Q,ZHAI H L,et al.An efficient approach to the quantitative analysis of humic acid in water[J].Food Chemistry,2016,190(1):1033-1039.
[8]ZHU L,WU H L,XIE L X,et al.A chemometrics-assisted excitation-emission matrix fluorescence method for simultaneous determination of arbutin and hydroquinone in cosmetic products[J].Analytical Methods,2016,8(24):4941-4948.
[9]吴文涛,陈宇男,肖雪,等.不同环境条件杂环农药三维荧光光谱发射特性研究[J].光谱学与光谱分析,2017,37(3):788-793.
[10]唐前进,邵杰,武志宗,等.激光诱导荧光传感器设计及其在毒品探测中的应用[J].机电一体化,2012,19(2):62-65.
[11]MAZINA J,ALEKSEJEV V,IVKINA T,et al.Qualitative detection of illegal drugs(cocaine,heroin and MDMA)in seized street samples based on SFS data and ANN:validation of method[J].Journal of Chemo metrics,2012,26(8-9):442-455.
[12]XU B Y,YE Y,LIAO L C.Detection of methamphetamine and morphine in urine and saliva using excitation-emission matrix fluorescence and a second-order calibration algorithm[J].Journal of Applied Spectroscopy,2016,83(3):383-391.
[13]BAHRAM M,BRO R,STEDMON C,et al.Handling of Rayleigh and Raman scatter for PARAFAC modeling of fluorescence data using interpolation[J].Journal of Chemometrics,2006,20(3-4):99-105.
[14]HARSHMAN R A.Foundations of the PARAFAC procedure:Models and conditions for an‘explanatory’multimodal factor analysis[D].UCLA Working Papers in Phonetics,1970,16(1):1-84.
[2]WIERGOWSKI M,ASZYK J,KALISZAN M,et al.Identification of novel psychoactive substances 25B-NBOMe and4-CMC in biological material using HPLC-Q-TOF-MS and their quantification in blood using UPLC-MS/MS in case of severe intoxications[J].Journal of Chromatography B,2017,1041-1042(1):1-10.
[3]GUIRGUIS A,GIROTTO S,BERTI B,et al.Identification of new psychoactive substances(NPS)using handheld Raman spectroscopy employing both 785 and 1064 nm laser sources[J].Forensic Science International,2017,273(4):113-123.
[4]孟鑫,刘培培,乔艳玲,等.尿液中甲卡西酮类新精神活性物质的GC-MS检测法[J].警察技术,2017(3):85-87.
[5]MAZINA J,SPILJOVA A,VAHER M,et al.A rapid capillary electrophoresis method with LED-induced native fluorescence detection for the analysis of cannabinoids in oral fluid[J].Analytical Methods,2015,7(18):7741-7747.
[6]王晓燕,陈仁文.典型食品果汁中百菌清农药残留荧光检测建模研究[J].光谱学与光谱分析,2017,27(3):794-798.
[7]WANG X,LI B Q,ZHAI H L,et al.An efficient approach to the quantitative analysis of humic acid in water[J].Food Chemistry,2016,190(1):1033-1039.
[8]ZHU L,WU H L,XIE L X,et al.A chemometrics-assisted excitation-emission matrix fluorescence method for simultaneous determination of arbutin and hydroquinone in cosmetic products[J].Analytical Methods,2016,8(24):4941-4948.
[9]吴文涛,陈宇男,肖雪,等.不同环境条件杂环农药三维荧光光谱发射特性研究[J].光谱学与光谱分析,2017,37(3):788-793.
[10]唐前进,邵杰,武志宗,等.激光诱导荧光传感器设计及其在毒品探测中的应用[J].机电一体化,2012,19(2):62-65.
[11]MAZINA J,ALEKSEJEV V,IVKINA T,et al.Qualitative detection of illegal drugs(cocaine,heroin and MDMA)in seized street samples based on SFS data and ANN:validation of method[J].Journal of Chemo metrics,2012,26(8-9):442-455.
[12]XU B Y,YE Y,LIAO L C.Detection of methamphetamine and morphine in urine and saliva using excitation-emission matrix fluorescence and a second-order calibration algorithm[J].Journal of Applied Spectroscopy,2016,83(3):383-391.
[13]BAHRAM M,BRO R,STEDMON C,et al.Handling of Rayleigh and Raman scatter for PARAFAC modeling of fluorescence data using interpolation[J].Journal of Chemometrics,2006,20(3-4):99-105.
[14]HARSHMAN R A.Foundations of the PARAFAC procedure:Models and conditions for an‘explanatory’multimodal factor analysis[D].UCLA Working Papers in Phonetics,1970,16(1):1-84.