AFP2基因调控拟南芥茉莉酸合成与开花时间的分析
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摘要
拟南芥AFPs (ABI five interaction proteins)是一类与植物激素脱落酸(ABA)信号途径中转录因子ABI5互作的蛋白,以前报道它们可以通过促进ABI5的降解来调控种子萌发,但是AFPs的新功能鲜有报道。我们发现过表达AFPs家族成员之一AFP2可以促进拟南芥小苗茉莉酸(JA)的合成并延迟开花,反之,拟南芥突变体afp2表现为较低的茉莉酸积累与较早的开花时间。突变茉莉酸合成酶LOX2或受体COI1都可以部分缓解AFP2-ox的晚花表型,同时过表达AFP2增强了AFP2类转录因子TOE1结合到成花素FT启动子区域的能力,并抑制FT的转录导致晚花。以上结果揭示了AFP2参与ABA与JA信号调控开花时间的新功能。
The AFPs is a kind of protei n that interact with transcriptional factor ABI5 in plant hormone ABA signal transduction pathway. Previous study showed that AFPs promote the degradation of ABI5 protein to regulate seed germination, but the novel function of AFP2 has been less reported so far. Here we found that the one component of AFPs protein could enhance the jasmonic acid biosynthesis and delayed flowering time, while the AFP2 mutant showed lower JA content and early flowering time. Mutation of the JA biosynthesis enzyme gene LOX2 or JA signal receptor COI1 could assuage the late-flowering phenotype of AFP2-ox. Meanwhile,overexpressing AFP2 enhanced the binding ability of TOE1 to the promoter of FT, and suppressed the transcriptional level of FT, ultimately delayed flowering time. These data might reveal the novel function of AFP2 in regulating ABA and JA mediated flowering time.
The AFPs is a kind of protei n that interact with transcriptional factor ABI5 in plant hormone ABA signal transduction pathway. Previous study showed that AFPs promote the degradation of ABI5 protein to regulate seed germination, but the novel function of AFP2 has been less reported so far. Here we found that the one component of AFPs protein could enhance the jasmonic acid biosynthesis and delayed flowering time, while the AFP2 mutant showed lower JA content and early flowering time. Mutation of the JA biosynthesis enzyme gene LOX2 or JA signal receptor COI1 could assuage the late-flowering phenotype of AFP2-ox. Meanwhile,overexpressing AFP2 enhanced the binding ability of TOE1 to the promoter of FT, and suppressed the transcriptional level of FT, ultimately delayed flowering time. These data might reveal the novel function of AFP2 in regulating ABA and JA mediated flowering time.
引文
Ben-Ari G.,2012,T he ABA signal tra nsduction mechanism in commercial crops:learning from Arabidopsis,Plant Cell Rep.,31(8):1357-1369
Chen R.,Jiang H.L.,Li L.,Zhai Q.Z.,Qi L.L.,Zhou W.K.,Liu X.Q.,Li H.M.,Zheng W.G.,Sun J.Q.,and Li C.Y.,2012,The Arabidopsis mediator subunit MED25 differentially regulates jasmonate and abscisic acid signaling through interacting with the MYC2 and ABI5 transcription factors,Plant Cell,24(7):2898-2916
Chiang G.C.K.,Barua D.,Kramer E.M.,Amasino R.M.,and Donohue K.,2009,Major flowering time gene,FLOWER-ING LOCUS C,regulates seed germination in Arabidopsis thaliana,Proc.Natl.Acad.Sci.USA,106(28):11661-11666
Cutler S.R.,Rodriguez P.L.,Finkelstein R.R.,and Abrams S.R.,2010,Abscisic acid:emergence of a core signaling network,Annu.Rev.Plant Biol.,61(1):651-679
Feng C.Z.,Chen Y.,Wang C.,Kong Y.H.,Wu W.H.,and Chen Y.F.,2014,Arabidopsis RAV1 transcription factor,phosphorylated by SnRK2 kinases,regulates the expression of ABI3,ABI4,and ABI5 during seed germination and early seedling development,Plant J.,80(4):654-668
Finkelstein R.R.,and Lynch T.J.,2000,The Arabidopsis abscisic acid response gene ABI5 encodes a basic leucine zipper transcription factor,Plant Cell,12(4):599-609
Fujii H.,Chinnusamy V.,Rodrigues A.,Rubio S.,Antoni R.,Park S.Y.,Cutler S.R.,Sheen J.,Rodriguez P.L.,and Zhu J.K.,2009,In vitro reconstitution of an abscisic acid signalling pathway,Nature,462(7273):660-664
Garcia M.E.,Lynch T.,Peeters J.,Snowden C.,and Finkelstein R.,2008,A small plant-specific protein family of ABI five binding proteins(AFPs)regulates stress response in germinating Arabidopsis seeds and seedlings,Plant Mol.Biol.,67(6):643-658
Huang X.,Zhang X.,Gong Z.,Yang S.,and Shi Y.,2017,ABI4represses the expression of type-A ARRs to inhibit seed germination in Arabidopsis,Plant J.,89(2):354-365
Jiang S.L.,Kumar S.,Eu Y.J.,Jami S.K.,Stasolla C.,and Hill R.D.,2012,The Arabidopsis mutant,fy-1,has an ABA-insensitive germination phenotype,J.Exp.Bot.,63(7):2693-2703
Lopez-Molina L.,Mongrand S.,Kinoshita N.,and Chua N.H.,2003,AFP is a novel negative regulator of ABA signaling that promotes ABI5 protein degradation,Gene Dev.,17(3):410-418
Lynch T.J.,Erickson B.J.,Miller D.R.,and Finkelstein R.R.,2017,ABI5-binding proteins(AFPs)alter transcription of ABA-induced genes via a variety of interactions with chromatin modifiers,Plant Mol.Biol.,93:403-418
Nishimura N.,Hitomi K.,Arvai A.S.,Rambo R.P.,Hitomi C.,Cutler S.R.,Schroeder J.I.,and Getzoff E.D.,2009,Structural mechanism of abscisic acid binding and signaling by dimeric PYR1,Science,326(5958):1373-1379
Ohnishi N.,Himi E.,Yamasaki Y.,and Noda K.,2008,Differential expression of three ABA-insensitive five binding protein(AFP)-like genes in wheat,Genes Genet.Syst.,83(2):167-177
Piskurewicz U.,Jikumaru Y.,Kinoshita N.,Nambara E.,Kamiya Y.,and Lopez-Molina L.,2008,The gibberellic acid signaling repressor RGL2 inhibits Arabidopsis seed germination by stimulating abscisic acid synthesis and ABI5 activity,Plant Cell,20(10):2729-2745
Shu K.,Chen Q.,Wu Y.R.,Liu R.J.,Zhang H.W.,Wang S.F.,Tang S.Y.,Yang W.Y.,and Xie Q.,2016,ABSCISICACID-INSENSITIVE 4 negatively regulates flowering through directly promoting Arabidopsis FLOWERING LO-CUS C transcription,J.Exp.Bot.,67(1):195-205
Tang N.,Ma S.,Zong W.,Yang N.,Lv Y.,Yan C.,Guo Z.,Li J.,Li X.,Xiang Y.,Song H.,Xiao J.,Li X.,and Xiong L.,2016,MODD mediates deactivation and degradation of OsbZIP46 to negatively regulate ABA signaling and drought resistance in rice,Plant Cell,28(9):2161-2177
Wang Y.P.,Li L.,Ye T.T.,Lu Y.M.,Chen X.,and Wu Y.,2013,The inhibitory effect of ABA on floral transition is mediated by ABI5 in Arabidopsis,J.Exp.Bot.,64(2):675-684
Weiner J.J.,Peterson F.C.,Volkman B.F.,and Cutler S.R.,2010,Structural and functional insights into core ABA signaling,Curr.Opin.Plant Biol.,13(5):495-502
Yang D.L.,Yao J.,Mei C.S.,Tong X.H.,Zeng L.J.,Li Q.,Xiao L.T.,Sun T.P.,Li J.,Deng X.W.,Lee C.M.,Thomashow M.F.,Yang Y.,He Z.,and He S.Y.,2012,Plant hormone jasmonate prioritizes defense over growth by interfering with gibberellin signaling cascade,Proc.Natl.Acad.Sci.USA,109(19):1192-1200
Yang L.,Li J.,Ji J.,Li P.,Yu L.,Abd Allah E.F.,Luo Y.,Hu L.,and Hu X.,2016,High temperature induces expression of tobacco transcription factor Nt MYC2a to regulate nicotine and JA biosynthesis,Front Physiol.,7:465
Zhai Q.,Zhang X.,Wu F.,Feng H.,Deng L.,Xu L.,Zhang M.,Wang Q.,and Li C.,2015,Transcriptional mechanism of jasmonate receptor COI1-mediated delay of flowering time in Arabidopsis,Plant Cell,27(10):2814-2828
Chen R.,Jiang H.L.,Li L.,Zhai Q.Z.,Qi L.L.,Zhou W.K.,Liu X.Q.,Li H.M.,Zheng W.G.,Sun J.Q.,and Li C.Y.,2012,The Arabidopsis mediator subunit MED25 differentially regulates jasmonate and abscisic acid signaling through interacting with the MYC2 and ABI5 transcription factors,Plant Cell,24(7):2898-2916
Chiang G.C.K.,Barua D.,Kramer E.M.,Amasino R.M.,and Donohue K.,2009,Major flowering time gene,FLOWER-ING LOCUS C,regulates seed germination in Arabidopsis thaliana,Proc.Natl.Acad.Sci.USA,106(28):11661-11666
Cutler S.R.,Rodriguez P.L.,Finkelstein R.R.,and Abrams S.R.,2010,Abscisic acid:emergence of a core signaling network,Annu.Rev.Plant Biol.,61(1):651-679
Feng C.Z.,Chen Y.,Wang C.,Kong Y.H.,Wu W.H.,and Chen Y.F.,2014,Arabidopsis RAV1 transcription factor,phosphorylated by SnRK2 kinases,regulates the expression of ABI3,ABI4,and ABI5 during seed germination and early seedling development,Plant J.,80(4):654-668
Finkelstein R.R.,and Lynch T.J.,2000,The Arabidopsis abscisic acid response gene ABI5 encodes a basic leucine zipper transcription factor,Plant Cell,12(4):599-609
Fujii H.,Chinnusamy V.,Rodrigues A.,Rubio S.,Antoni R.,Park S.Y.,Cutler S.R.,Sheen J.,Rodriguez P.L.,and Zhu J.K.,2009,In vitro reconstitution of an abscisic acid signalling pathway,Nature,462(7273):660-664
Garcia M.E.,Lynch T.,Peeters J.,Snowden C.,and Finkelstein R.,2008,A small plant-specific protein family of ABI five binding proteins(AFPs)regulates stress response in germinating Arabidopsis seeds and seedlings,Plant Mol.Biol.,67(6):643-658
Huang X.,Zhang X.,Gong Z.,Yang S.,and Shi Y.,2017,ABI4represses the expression of type-A ARRs to inhibit seed germination in Arabidopsis,Plant J.,89(2):354-365
Jiang S.L.,Kumar S.,Eu Y.J.,Jami S.K.,Stasolla C.,and Hill R.D.,2012,The Arabidopsis mutant,fy-1,has an ABA-insensitive germination phenotype,J.Exp.Bot.,63(7):2693-2703
Lopez-Molina L.,Mongrand S.,Kinoshita N.,and Chua N.H.,2003,AFP is a novel negative regulator of ABA signaling that promotes ABI5 protein degradation,Gene Dev.,17(3):410-418
Lynch T.J.,Erickson B.J.,Miller D.R.,and Finkelstein R.R.,2017,ABI5-binding proteins(AFPs)alter transcription of ABA-induced genes via a variety of interactions with chromatin modifiers,Plant Mol.Biol.,93:403-418
Nishimura N.,Hitomi K.,Arvai A.S.,Rambo R.P.,Hitomi C.,Cutler S.R.,Schroeder J.I.,and Getzoff E.D.,2009,Structural mechanism of abscisic acid binding and signaling by dimeric PYR1,Science,326(5958):1373-1379
Ohnishi N.,Himi E.,Yamasaki Y.,and Noda K.,2008,Differential expression of three ABA-insensitive five binding protein(AFP)-like genes in wheat,Genes Genet.Syst.,83(2):167-177
Piskurewicz U.,Jikumaru Y.,Kinoshita N.,Nambara E.,Kamiya Y.,and Lopez-Molina L.,2008,The gibberellic acid signaling repressor RGL2 inhibits Arabidopsis seed germination by stimulating abscisic acid synthesis and ABI5 activity,Plant Cell,20(10):2729-2745
Shu K.,Chen Q.,Wu Y.R.,Liu R.J.,Zhang H.W.,Wang S.F.,Tang S.Y.,Yang W.Y.,and Xie Q.,2016,ABSCISICACID-INSENSITIVE 4 negatively regulates flowering through directly promoting Arabidopsis FLOWERING LO-CUS C transcription,J.Exp.Bot.,67(1):195-205
Tang N.,Ma S.,Zong W.,Yang N.,Lv Y.,Yan C.,Guo Z.,Li J.,Li X.,Xiang Y.,Song H.,Xiao J.,Li X.,and Xiong L.,2016,MODD mediates deactivation and degradation of OsbZIP46 to negatively regulate ABA signaling and drought resistance in rice,Plant Cell,28(9):2161-2177
Wang Y.P.,Li L.,Ye T.T.,Lu Y.M.,Chen X.,and Wu Y.,2013,The inhibitory effect of ABA on floral transition is mediated by ABI5 in Arabidopsis,J.Exp.Bot.,64(2):675-684
Weiner J.J.,Peterson F.C.,Volkman B.F.,and Cutler S.R.,2010,Structural and functional insights into core ABA signaling,Curr.Opin.Plant Biol.,13(5):495-502
Yang D.L.,Yao J.,Mei C.S.,Tong X.H.,Zeng L.J.,Li Q.,Xiao L.T.,Sun T.P.,Li J.,Deng X.W.,Lee C.M.,Thomashow M.F.,Yang Y.,He Z.,and He S.Y.,2012,Plant hormone jasmonate prioritizes defense over growth by interfering with gibberellin signaling cascade,Proc.Natl.Acad.Sci.USA,109(19):1192-1200
Yang L.,Li J.,Ji J.,Li P.,Yu L.,Abd Allah E.F.,Luo Y.,Hu L.,and Hu X.,2016,High temperature induces expression of tobacco transcription factor Nt MYC2a to regulate nicotine and JA biosynthesis,Front Physiol.,7:465
Zhai Q.,Zhang X.,Wu F.,Feng H.,Deng L.,Xu L.,Zhang M.,Wang Q.,and Li C.,2015,Transcriptional mechanism of jasmonate receptor COI1-mediated delay of flowering time in Arabidopsis,Plant Cell,27(10):2814-2828