湿性疗法对创面表皮干细胞影响的研究
|
摘要
目的研究分析湿性疗法对临床常见创面的促进愈合作用,并观察表皮干细胞变化。方法选择我院2010年2月~2012年3月收治的40例患者为研究对象,随机分为两组,对照组给予常规治疗,试验组患者均给予湿性疗法,观察创面愈合情况,计算愈合天数。取试验开始时、试验中期和结束时创面组织,制作HE染色切片,观察创面发生的变化;检测试验开始时、试验中期和结束时β1整合素的变化情况。结果试验组患者愈合天数少,与对照组比较有显著差异(P<0.05);试验组β1整合素明显高于对照组,组间比较有显著差异(P<0.05)。结论湿性疗法对创面愈合具有促进作用,可以促进表皮干细胞的生长,值得应用。
Objective To study and analyze the effect of moist therapy in promoting common clinical wound healing,and observe the changes of epidermal stem cells.Methods The 40 cases in our hospital from February 2010 to March2012 were selected as study objects and randomly divided into two groups,the control group was given conventional treatment,the test group was given moist therapy.Observe the wound healing situation and calculated healing days.Taken the test beginning,middle and end of the test wound tissue,made HE staining to observe the wound changes.Teste the changes of β1 integrin at the beginning,the middle and end of the test.Results The healed days of the test group was shorter,compared with the control group,it had significant difference(P<0.05);the β1 integrin of the test group was obviously higher than the control group,there were significant differences between the two groups(P<0.05).Conclusion Moist therapy has a role in promoting wound healing,it can promote the growth of epidermal stem cells,it is worthy for application.
Objective To study and analyze the effect of moist therapy in promoting common clinical wound healing,and observe the changes of epidermal stem cells.Methods The 40 cases in our hospital from February 2010 to March2012 were selected as study objects and randomly divided into two groups,the control group was given conventional treatment,the test group was given moist therapy.Observe the wound healing situation and calculated healing days.Taken the test beginning,middle and end of the test wound tissue,made HE staining to observe the wound changes.Teste the changes of β1 integrin at the beginning,the middle and end of the test.Results The healed days of the test group was shorter,compared with the control group,it had significant difference(P<0.05);the β1 integrin of the test group was obviously higher than the control group,there were significant differences between the two groups(P<0.05).Conclusion Moist therapy has a role in promoting wound healing,it can promote the growth of epidermal stem cells,it is worthy for application.
引文
[1]李永涛,王喜梅,乔晓俊,等.糖尿病创面表皮干细胞增殖分化相关蛋白的研究[J].中国美容医学,2010,19(8):1171-1172.
[2]柴琳琳,曹川,赵树文,等.Rac1蛋白调控表皮干细胞迁移促进创面愈合的研究[J].中华烧伤杂志,2011,27(3):205-209.
[3]宋宁,富玲,辛畅泰,等.皮肤全层缺损真皮修复不同阶段对表皮再生的影响[J].中国组织工程研究与临床康复,2010,14(41):7631-7634.
[4]毛庆龙,梁自乾.深Ⅱ度烧伤创面磨痂治疗后残留皮肤组织中表皮干细胞标记物角化蛋白19的表达[J].中国组织工程研究与临床康复,2009,13(27):5301-5304.
[5]钟清玲.羊膜负载表皮干细胞促进糖尿病大鼠创面的愈合[J].中国组织工程研究与临床康复,2010,25(32):167-168
[6]李建福,付小兵,盛志勇,等.创面愈合过程中表皮干细胞的分布特征及意义[J].解放军医学杂志,2010,11(7):223.
[7]付小兵.进一步重视体表慢性难愈合创面发生机制与防治研究[J].中华创伤杂志,2004,20(8):449-451.
[8]李建福,付小兵,盛志勇.创面愈合过程中创缘表皮干细胞的异位[J].中国修复重建外科杂志,2006,20(3):264-267.
[9]Oberleya C,Gourronca F,Hakimia S.Murine epidermal side population possesses unique angiogenic properties[J].Experimental Cell Research,2008,314(4):720-728.
[10]田晓红,柏树令,田伟.表皮干细胞在糖尿病创面愈合过程中的动态变化[J].中国修复重建外科杂志,2007,21(7):693-697.
[11]付小兵,李建福,盛志勇.表皮干细胞:实现创面由解剖修复到功能修复飞跃的新策略[J].中华烧伤杂志,2003,19(1):5-7.
[2]柴琳琳,曹川,赵树文,等.Rac1蛋白调控表皮干细胞迁移促进创面愈合的研究[J].中华烧伤杂志,2011,27(3):205-209.
[3]宋宁,富玲,辛畅泰,等.皮肤全层缺损真皮修复不同阶段对表皮再生的影响[J].中国组织工程研究与临床康复,2010,14(41):7631-7634.
[4]毛庆龙,梁自乾.深Ⅱ度烧伤创面磨痂治疗后残留皮肤组织中表皮干细胞标记物角化蛋白19的表达[J].中国组织工程研究与临床康复,2009,13(27):5301-5304.
[5]钟清玲.羊膜负载表皮干细胞促进糖尿病大鼠创面的愈合[J].中国组织工程研究与临床康复,2010,25(32):167-168
[6]李建福,付小兵,盛志勇,等.创面愈合过程中表皮干细胞的分布特征及意义[J].解放军医学杂志,2010,11(7):223.
[7]付小兵.进一步重视体表慢性难愈合创面发生机制与防治研究[J].中华创伤杂志,2004,20(8):449-451.
[8]李建福,付小兵,盛志勇.创面愈合过程中创缘表皮干细胞的异位[J].中国修复重建外科杂志,2006,20(3):264-267.
[9]Oberleya C,Gourronca F,Hakimia S.Murine epidermal side population possesses unique angiogenic properties[J].Experimental Cell Research,2008,314(4):720-728.
[10]田晓红,柏树令,田伟.表皮干细胞在糖尿病创面愈合过程中的动态变化[J].中国修复重建外科杂志,2007,21(7):693-697.
[11]付小兵,李建福,盛志勇.表皮干细胞:实现创面由解剖修复到功能修复飞跃的新策略[J].中华烧伤杂志,2003,19(1):5-7.